##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935499.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675889
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11314727714166	31.0	31.0	33.0	28.0	34.0
2	31.15455940250544	31.0	31.0	34.0	28.0	34.0
3	30.945705581833703	31.0	30.0	34.0	27.0	34.0
4	34.73818482028854	35.0	35.0	37.0	32.0	37.0
5	34.72700842889883	35.0	35.0	37.0	32.0	37.0
6	34.905499275768655	35.0	35.0	37.0	32.0	37.0
7	35.21483705164606	36.0	35.0	37.0	33.0	37.0
8	34.95172728066295	37.0	35.0	37.0	32.0	37.0
9	36.70707912097993	39.0	37.0	39.0	32.0	39.0
10	36.249523220528815	37.0	35.0	39.0	32.0	39.0
11	36.58843981778073	38.0	35.0	39.0	32.0	39.0
12	36.69990338650281	39.0	35.0	39.0	33.0	39.0
13	36.682797027322536	39.0	35.0	39.0	32.0	39.0
14	37.727171177515835	40.0	37.0	41.0	33.0	41.0
15	37.814846816563076	40.0	37.0	41.0	33.0	41.0
16	37.924008232120954	40.0	37.0	41.0	33.0	41.0
17	37.699179894923574	39.0	36.0	41.0	33.0	41.0
18	37.639078310195906	39.0	36.0	40.0	33.0	41.0
19	37.442531244035635	39.0	36.0	40.0	33.0	41.0
20	37.15493520385744	39.0	35.0	40.0	32.0	41.0
21	37.330589786192704	39.0	35.0	40.0	33.0	41.0
22	37.42445283175196	39.0	35.0	41.0	33.0	41.0
23	37.4293071791374	39.0	35.0	41.0	33.0	41.0
24	37.419355840973886	39.0	35.0	41.0	33.0	41.0
25	37.176779027325495	39.0	35.0	41.0	32.0	41.0
26	37.121861725815926	39.0	35.0	41.0	32.0	41.0
27	37.11671738998563	39.0	35.0	41.0	32.0	41.0
28	36.97092126073956	39.0	35.0	41.0	32.0	41.0
29	36.96846375662276	39.0	35.0	41.0	32.0	41.0
30	36.63052069200712	39.0	35.0	41.0	31.0	41.0
31	36.53855292806955	39.0	35.0	41.0	30.0	41.0
32	36.257558563610296	39.0	35.0	41.0	30.0	41.0
33	36.01915551222168	39.0	35.0	41.0	29.0	41.0
34	35.674058906122156	39.0	35.0	41.0	26.0	41.0
35	35.66485473206399	39.0	35.0	41.0	26.0	41.0
36	35.38019704418921	39.0	35.0	41.0	24.0	41.0
37	35.330971505676224	39.0	35.0	41.0	24.0	41.0
38	35.15986500741986	38.0	35.0	40.0	24.0	41.0
39	35.061328117486745	38.0	35.0	40.0	23.0	41.0
40	34.90269260189173	38.0	34.0	40.0	23.0	41.0
41	34.701674387362424	38.0	34.0	40.0	21.0	41.0
42	34.798992142200866	38.0	34.0	40.0	22.0	41.0
43	34.80733818718754	38.0	35.0	40.0	22.0	41.0
44	34.88783809175767	38.0	35.0	40.0	22.0	41.0
45	34.96602548643342	38.0	35.0	40.0	23.0	41.0
46	34.879858970925696	38.0	35.0	40.0	23.0	41.0
47	34.723129093682545	38.0	34.0	40.0	23.0	41.0
48	34.76024169649158	38.0	34.0	40.0	23.0	41.0
49	34.76776660072882	38.0	34.0	40.0	23.0	41.0
50	34.58979802896629	38.0	34.0	40.0	22.0	41.0
51	33.43904250550016	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	27.0
10	34.0
11	29.0
12	14.0
13	32.0
14	27.0
15	39.0
16	80.0
17	145.0
18	356.0
19	825.0
20	1778.0
21	2964.0
22	4122.0
23	5614.0
24	7454.0
25	9796.0
26	11985.0
27	12135.0
28	11590.0
29	11697.0
30	12905.0
31	16060.0
32	21105.0
33	28966.0
34	46203.0
35	51290.0
36	53516.0
37	78209.0
38	134612.0
39	152240.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.21202593917049	17.136541651069926	19.404813512277904	17.246618897481685
2	36.327710615204566	29.404976260894912	19.91658393611969	14.350729187780834
3	26.63558661259467	29.578525467939258	29.498630692317825	14.287257227148245
4	23.977161930435322	20.470521047095012	38.86969605956008	16.68262096290959
5	27.56547302885533	24.72713714825955	29.175500710915546	18.53188911196957
6	20.947078588348088	34.442341863826755	31.22435784574094	13.386221702084217
7	66.83316343364073	4.900804717934454	23.463320160558908	4.802711687865907
8	65.78313894737153	10.216914315812211	17.861364809902216	6.138581926914035
9	60.398822883639184	5.794886438453652	18.96805540554736	14.838235272359809
10	37.1592080948203	18.320315909860938	28.105946390605556	16.4145296047132
11	29.30362825848623	21.46905778907483	30.00862567670135	19.218688275737584
12	22.71408470917562	18.15135325475041	35.99392799705277	23.140634039021197
13	24.03811868516872	19.323883063639148	39.927414116815044	16.710584134377093
14	18.492829443887977	26.65437668019453	35.37770255175036	19.475091324167135
15	16.558784060696357	22.864109343398102	40.70875543173509	19.86835116417045
16	19.937445349754178	24.049215181782806	32.296427371950124	23.71691209651289
17	19.45275037765077	25.98281670510986	33.207671673899114	21.35676124334025
18	19.985678121703415	23.646190424759094	33.67668359745461	22.691447856082878
19	19.117636179905283	25.875994431038237	31.073001631924768	23.93336775713172
20	21.0151371009145	26.343970681576412	34.16862828067922	18.472263936829865
21	19.495360924648867	28.808724509497864	34.408460560831735	17.287454005021534
22	18.207575504261793	21.71599182706036	36.51309608530395	23.563336583373896
23	19.948541846368265	25.158568936615332	34.805715139616126	20.087174077400284
24	21.15702430428665	24.86073896749318	31.925952338327747	22.056284389892422
25	19.092336167625156	29.81391914944614	29.982881804556666	21.110862878372043
26	18.369288448251115	22.677392293705033	34.59798872300037	24.355330535043475
27	23.67060271731009	22.988686012052277	31.338725737510153	22.00198553312748
28	17.98534966540364	24.242738082732522	34.88664558825488	22.885266663608967
29	20.676176117676125	21.478378846230665	35.79300743169366	22.05243760439954
30	22.631674727654985	24.902757701338533	33.574299922028615	18.891267648977863
31	21.42126887699016	22.601492256864663	33.231344200009175	22.745894666136007
32	23.264914801098996	24.983244290112726	30.03170638965866	21.72013451912962
33	22.528403332499863	25.909431874168686	28.528796888246443	23.033367905085004
34	19.237922203202004	27.4318712096217	32.03262665911119	21.29757992806511
35	21.798105902004618	28.67512269026423	28.36885938371537	21.157912024015776
36	22.939861426950284	28.173708996595593	30.554425356826343	18.33200421962778
37	21.447012749134846	26.1723448672785	30.97727585446723	21.403366529119427
38	20.294456634151466	27.45731917519001	28.834616334930736	23.41360785572779
39	22.318161709984924	26.171605100837564	28.17311718344284	23.33711600573467
40	21.02889675671597	27.20786993130529	30.911880501088195	20.851352810890546
41	20.674252724929683	27.829421694982457	28.89424151007044	22.602084070017415
42	20.636968496306345	25.53422233532429	32.80168785111165	21.027121317257716
43	24.33861181347825	22.928616977048005	29.83581623609794	22.896954973375806
44	21.746470204427055	22.335176338126526	30.778574588430942	25.139778869015473
45	19.907410832252044	22.09874698360234	30.1018362482597	27.892005935885923
46	24.42679197323821	24.304582557195044	29.237641091954448	22.0309843776123
47	18.962285227308033	23.805388162849226	35.17796561269676	22.05436099714598
48	19.727943493680176	24.814429588290384	31.099485270510396	24.358141647519048
49	19.803251717367793	21.672789466909506	35.57758744409215	22.94637137163055
50	20.327894077281922	22.05036625836491	33.52281217773924	24.098927486613928
51	19.308791828243987	21.75312810239551	31.21059818993947	27.727481879421028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	276.0
1	525.5
2	775.0
3	9638.5
4	18502.0
5	11798.5
6	5095.0
7	4895.5
8	4696.0
9	4618.0
10	4540.0
11	4525.5
12	4511.0
13	4362.5
14	4214.0
15	4007.5
16	3801.0
17	3616.0
18	3431.0
19	3224.5
20	3018.0
21	2948.5
22	2879.0
23	2960.0
24	3041.0
25	3562.5
26	4524.5
27	4965.0
28	5409.0
29	5853.0
30	6978.5
31	8104.0
32	9683.5
33	11263.0
34	12837.5
35	14412.0
36	15774.5
37	17137.0
38	18788.5
39	20440.0
40	23040.5
41	25641.0
42	29844.0
43	34047.0
44	67454.5
45	100862.0
46	82834.0
47	64806.0
48	65521.0
49	66236.0
50	58168.0
51	50100.0
52	44439.5
53	38779.0
54	36097.5
55	33416.0
56	31192.5
57	28969.0
58	26635.0
59	24301.0
60	21727.0
61	19153.0
62	16616.5
63	14080.0
64	12197.5
65	10315.0
66	8831.0
67	7347.0
68	6178.0
69	5009.0
70	4092.5
71	3176.0
72	2551.0
73	1926.0
74	1596.0
75	1041.5
76	817.0
77	561.5
78	306.0
79	217.5
80	129.0
81	107.0
82	85.0
83	64.5
84	44.0
85	35.0
86	26.0
87	16.0
88	6.0
89	6.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	675889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.960345761535585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.12890197736739	30.021768997001157
2	9.386273020518582	7.501574306241911
3	3.7632522566357003	4.51142584089125
4	2.207826340707839	3.5290201582444447
5	1.59491436697934	3.1866664782267544
6	1.2388765973032383	2.970356231046725
7	1.0225134257793047	2.860199302796729
8	0.8421702097759968	2.692273021817103
9	0.7129257406073998	2.5639883187273473
>10	4.027867633985944	24.56827196342294
>50	0.04154324528241312	1.1469524541903025
>100	0.024701388792621952	1.8667701656251792
>500	0.0014970538662195116	0.40185959261615567
>1k	0.004865425065213412	3.9741775044991092
>5k	0.0011227903996646337	3.5974434837324187
>10k+	7.485269331097558E-4	4.607252180920467
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	18315	2.7097644731605337	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12491	1.848084522754476	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	9387	1.3888375162193793	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	9193	1.3601345783109355	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	5474	0.8098962995403091	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	4635	0.6857634907507002	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	3046	0.4506657158202012	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	2366	0.350057479852461	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	2298	0.3399966562556869	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	2137	0.3161761768574426	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	2128	0.3148445972637519	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT	1906	0.28199896728604845	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	1725	0.25521942212404697	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA	1507	0.22296560529909498	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	1442	0.21334864156688452	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	1216	0.17991119843642966	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	1108	0.16393224331214148	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1059	0.15668253219093667	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	828	0.12250532261954256	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	690	0.10208776884961879	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.959065763757067E-4	0.0	0.0	0.17162581429790985	0.0
2	2.959065763757067E-4	0.0	0.0	1.1454543571503606	0.0
3	2.959065763757067E-4	0.0	0.0	1.6933253833099813	0.0
4	2.959065763757067E-4	0.0	0.0	3.025496790153413	0.0
5	2.959065763757067E-4	0.0	0.0	6.955432030999173	0.0
6	2.959065763757067E-4	0.0	0.0	8.835326510714037	0.0
7	2.959065763757067E-4	0.0	0.0	10.2328932709365	0.0
8	2.959065763757067E-4	0.0	0.0	11.851058383847052	0.0
9	2.959065763757067E-4	0.0	0.0	12.568335924981765	0.0
10	2.959065763757067E-4	0.0	0.0	15.059721344777026	0.0
11	2.959065763757067E-4	0.0	0.0	16.374286310326102	0.0
12	2.959065763757067E-4	0.0	0.0	18.83918809153574	0.0
13	2.959065763757067E-4	0.0	0.0	19.475091324167135	0.0
14	2.959065763757067E-4	0.0	0.0	19.86346870566025	0.0
15	2.959065763757067E-4	0.0	0.0	20.4182935363647	0.0
16	2.959065763757067E-4	0.0	0.0	21.03437102837892	0.0
17	2.959065763757067E-4	0.0	0.0	21.674860812944136	0.0
18	2.959065763757067E-4	0.0	0.0	22.37911846471832	0.0
19	2.959065763757067E-4	0.0	0.0	23.124507130608723	0.0
20	2.959065763757067E-4	0.0	0.0	23.61615590725696	0.0
21	2.959065763757067E-4	0.0	0.0	24.14198189347659	0.0
22	2.959065763757067E-4	0.0	0.0	24.740748850772835	0.0
23	2.959065763757067E-4	0.0	0.0	25.277671333606555	0.0
24	2.959065763757067E-4	0.0	0.0	25.714577393625284	0.0
25	2.959065763757067E-4	0.0	0.0	26.132693386044156	0.0
26	2.959065763757067E-4	0.0	0.0	26.580843895965167	0.0
27	5.918131527514134E-4	0.0	0.0	27.03816750975382	0.0
28	5.918131527514134E-4	0.0	0.0	27.447406304881422	0.0
29	5.918131527514134E-4	0.0	0.0	27.893633422055988	0.0
30	5.918131527514134E-4	0.0	0.0	28.49728283786243	0.0
31	5.918131527514134E-4	0.0	0.0	28.970733360063562	0.0
32	5.918131527514134E-4	0.0	0.0	29.44758680789301	0.0
33	7.397664409392666E-4	0.0	0.0	29.930358387249978	0.0
34	7.397664409392666E-4	0.0	0.0	30.3893094872087	0.0
35	7.397664409392666E-4	0.0	0.0	30.873412646159355	0.0
36	7.397664409392666E-4	0.0	0.0	31.317420464011104	0.0
37	7.397664409392666E-4	0.0	0.0	31.775631797528884	0.0
38	7.397664409392666E-4	0.0	0.0	32.30515661595321	0.0
39	7.397664409392666E-4	0.0	0.0	32.97997156337801	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	20	7.0312206E-4	45.000004	1
CGTCGAA	35	1.2109376E-7	45.0	42
TTCGACG	25	3.889094E-5	45.0	1
AGTTACG	30	2.16417E-6	44.999996	1
CGTTTTT	5405	0.0	43.6679	1
CGATGAA	1255	0.0	43.565735	19
CCGATGA	1260	0.0	42.678574	18
GTCGAAT	170	0.0	42.35294	43
CACCGAT	1345	0.0	41.82156	16
CGACGGT	70	0.0	41.785713	28
CGTTTTC	180	0.0	41.25	1
GATGAAT	1375	0.0	40.909092	20
TACGGCT	1160	0.0	40.53879	7
CTCGTAA	50	1.0804797E-9	40.5	33
TCGTAAC	50	1.0804797E-9	40.5	34
GCTCGTA	50	1.0804797E-9	40.5	32
ACCGATG	1340	0.0	40.298504	17
CGGCGAA	185	0.0	40.135136	31
CCGTCGA	45	1.9263098E-8	40.0	41
GCGATCT	45	1.9263098E-8	40.0	9
>>END_MODULE