##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935495.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250082
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13806271542934	31.0	31.0	33.0	28.0	34.0
2	31.131200966083124	31.0	31.0	34.0	28.0	34.0
3	30.95033628969698	31.0	30.0	34.0	27.0	34.0
4	34.79094057149255	35.0	35.0	37.0	32.0	37.0
5	34.80565574491567	35.0	35.0	37.0	32.0	37.0
6	35.00367079597892	36.0	35.0	37.0	32.0	37.0
7	35.459037435721086	37.0	35.0	37.0	33.0	37.0
8	35.13955822490223	37.0	35.0	37.0	32.0	37.0
9	36.97734343135451	39.0	37.0	39.0	32.0	39.0
10	36.32991978631009	37.0	35.0	39.0	32.0	39.0
11	36.65911980870275	38.0	35.0	39.0	32.0	39.0
12	36.78886125350885	39.0	35.0	39.0	33.0	39.0
13	36.83484217176766	39.0	37.0	39.0	33.0	39.0
14	37.809286554010285	39.0	37.0	41.0	33.0	41.0
15	38.03168560712086	40.0	37.0	41.0	33.0	41.0
16	37.94230692332915	39.0	37.0	41.0	33.0	41.0
17	37.93388968418359	39.0	37.0	41.0	33.0	41.0
18	37.917047208515605	39.0	37.0	41.0	33.0	41.0
19	37.78018809830375	39.0	37.0	41.0	33.0	41.0
20	37.915963563951024	39.0	36.0	41.0	34.0	41.0
21	37.85701090042466	39.0	36.0	41.0	33.0	41.0
22	38.00263913436393	40.0	37.0	41.0	34.0	41.0
23	38.04611687366544	40.0	37.0	41.0	34.0	41.0
24	38.0300621396182	40.0	37.0	41.0	34.0	41.0
25	37.61654977167489	39.0	36.0	41.0	33.0	41.0
26	37.74561143944786	39.0	36.0	41.0	33.0	41.0
27	37.790024871842036	40.0	36.0	41.0	33.0	41.0
28	37.73023648243376	40.0	36.0	41.0	33.0	41.0
29	37.71375788741293	40.0	36.0	41.0	33.0	41.0
30	37.44949656512664	39.0	36.0	41.0	33.0	41.0
31	37.43844019161715	39.0	36.0	41.0	33.0	41.0
32	37.44952455594565	39.0	36.0	41.0	33.0	41.0
33	37.453811149942815	40.0	36.0	41.0	33.0	41.0
34	37.393758847098155	40.0	36.0	41.0	33.0	41.0
35	37.41398021448965	40.0	36.0	41.0	33.0	41.0
36	37.265600882910405	40.0	36.0	41.0	32.0	41.0
37	37.24414392079398	40.0	36.0	41.0	32.0	41.0
38	37.103430074935424	39.0	35.0	41.0	32.0	41.0
39	37.036691965035466	39.0	35.0	41.0	32.0	41.0
40	36.955114722371064	39.0	35.0	41.0	31.0	41.0
41	36.7944034356731	39.0	35.0	41.0	31.0	41.0
42	36.84282755256276	39.0	35.0	41.0	31.0	41.0
43	36.80046944602171	39.0	35.0	41.0	31.0	41.0
44	36.76786014187346	39.0	35.0	41.0	31.0	41.0
45	36.75116961636583	39.0	35.0	41.0	31.0	41.0
46	36.64430466806888	39.0	35.0	41.0	31.0	41.0
47	36.61184331539255	39.0	35.0	41.0	31.0	41.0
48	36.545924936620786	39.0	35.0	41.0	31.0	41.0
49	36.55316256267944	39.0	35.0	41.0	31.0	41.0
50	36.422905287065845	39.0	35.0	40.0	31.0	41.0
51	35.18477139498245	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	13.0
10	12.0
11	12.0
12	7.0
13	13.0
14	6.0
15	5.0
16	13.0
17	34.0
18	53.0
19	131.0
20	212.0
21	348.0
22	555.0
23	794.0
24	1092.0
25	1469.0
26	1834.0
27	2233.0
28	2501.0
29	2979.0
30	3690.0
31	4960.0
32	6653.0
33	9533.0
34	17232.0
35	18904.0
36	20460.0
37	31896.0
38	57562.0
39	64857.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36911892899129	20.23736214521637	22.538607336793532	11.854911588998808
2	34.4582976783615	23.561471837237384	23.575467246743067	18.40476323765805
3	32.83363056917331	23.240377156292734	26.797210514951097	17.128781759582857
4	28.997288889244327	25.222527011140343	26.279780232083876	19.50040386753145
5	24.59113410801257	30.22088754888397	25.387272974464377	19.800705368639086
6	24.7086955478603	35.26163418398765	25.26331363312833	14.766356635023712
7	77.15189417870938	6.418294799305828	11.125950688174278	5.30386033381051
8	75.63359218176439	6.888140689853728	10.617717388696507	6.860549739685383
9	69.15771626906376	8.818307595108804	13.467982501739428	8.555993634088018
10	37.610463767884134	23.096424372805718	22.47742740381155	16.815684455498598
11	29.63028126774418	25.91270063419199	24.666709319343255	19.79030877872058
12	25.039786949880437	22.88609336137747	30.656744587775208	21.417375100966883
13	24.202461592597626	24.877440199614526	31.786374069305268	19.133724138482577
14	20.473284762597867	28.137570876752427	29.791828280324058	21.597316080325655
15	19.560784062827395	25.43925592405691	35.29122447837109	19.708735534744605
16	20.9355331451284	25.38087507297606	31.46407978183156	22.21951200006398
17	20.57525131756784	25.755952047728346	28.84054030278069	24.82825633192313
18	22.075559216576963	24.895434297550402	31.621628105981237	21.407378379891394
19	22.14153757567518	26.636863108900283	29.02088115098248	22.200718164442062
20	22.60338608936269	27.129901392343314	29.849009524875843	20.417702993418157
21	22.36066570164986	27.411009188986014	29.87380139314305	20.354523716221077
22	19.97144936460841	26.17461472636975	29.1916251469518	24.662310762070042
23	19.997440839404675	27.063123295559055	29.119248886365273	23.820186978670996
24	20.993913996209244	26.091442007021698	29.44634159995522	23.468302396813844
25	19.64075783143129	26.514103374093295	28.83174318823426	25.01339560624115
26	19.903871530138115	27.014739165553696	29.767836149742884	23.3135531545653
27	20.888748490495118	28.146367991298852	28.364696379587496	22.600187138618534
28	18.45754592493662	26.585280028150766	30.268471941203284	24.68870210570933
29	21.578122375860715	26.446125670779985	28.776561287897568	23.199190665461728
30	21.695683815708446	27.048328148367336	27.82767252341232	23.428315512511897
31	21.162658647963468	29.464735566734113	24.951016066730112	24.42158971857231
32	22.136339280715926	30.869874681104598	24.903831543253812	22.089954494925664
33	20.081413296438768	29.178429475132155	25.253716780895868	25.48644044753321
34	19.58597579993762	28.465063459185387	27.235066898057436	24.713893842819555
35	20.475683975655983	29.73984533073152	25.475643988771683	24.308826704840815
36	21.374989003606817	30.442015019073743	25.231324125686776	22.951671851632664
37	19.591973832582912	31.631624827056726	26.468918194832096	22.307483145528266
38	18.702265656864547	31.458081749186267	24.72868899001128	25.110963603937908
39	20.513271646899817	28.842939515838808	26.541294455418623	24.102494381842757
40	20.715605281467678	27.768491934645432	27.73650242720388	23.77940035668301
41	18.494733727337433	30.47880295263154	25.61359873961341	25.412864580417622
42	21.37818795435097	28.64420470085812	26.298973936548816	23.678633408242096
43	21.23943346582321	27.675722363064914	27.089114770355323	23.995729400756552
44	20.382514535232442	26.45452291648339	26.662454714853528	26.500507833430635
45	20.58284882558521	26.348557673083228	26.131828760166663	26.936764741164897
46	21.89041994225894	27.655329052070922	26.41013747490823	24.04411353076191
47	19.160115482121864	27.967626618469144	28.82174646715877	24.05051143225022
48	19.130125318895402	27.668924592733585	27.439399876840397	25.761550211530622
49	20.680016954438944	25.639190345566654	29.781831559248566	23.898961140745836
50	20.202573555873673	24.597931878343903	28.920514071384584	26.278980494397835
51	19.086539615006277	25.147351668652686	26.45212370342528	29.31398501291576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	203.0
2	286.0
3	811.5
4	1337.0
5	910.5
6	484.0
7	488.5
8	493.0
9	489.0
10	485.0
11	514.5
12	544.0
13	546.0
14	548.0
15	558.0
16	568.0
17	582.5
18	597.0
19	600.0
20	603.0
21	662.5
22	722.0
23	789.0
24	856.0
25	999.5
26	1383.0
27	1623.0
28	2128.5
29	2634.0
30	3025.0
31	3416.0
32	3870.0
33	4324.0
34	4775.5
35	5227.0
36	5969.0
37	6711.0
38	7708.5
39	8706.0
40	10281.0
41	11856.0
42	14199.0
43	16542.0
44	21076.5
45	25611.0
46	27882.5
47	30154.0
48	30547.0
49	30940.0
50	26593.5
51	22247.0
52	19640.0
53	17033.0
54	15241.0
55	13449.0
56	11781.5
57	10114.0
58	9518.5
59	8923.0
60	7955.0
61	6987.0
62	6226.0
63	5465.0
64	4461.0
65	3457.0
66	2842.0
67	2227.0
68	1765.0
69	1303.0
70	1168.0
71	1033.0
72	861.5
73	690.0
74	504.5
75	241.5
76	164.0
77	99.0
78	34.0
79	45.5
80	57.0
81	47.5
82	38.0
83	21.5
84	5.0
85	3.5
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	250082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.98379765193547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57270036312154	29.743048667367166
2	11.363213610421981	9.31415294570507
3	3.941096530499027	4.845633081981511
4	2.112821340609795	3.463657691929714
5	1.4301689407512677	2.9306877237916416
6	1.0753733435130337	2.6443730106494083
7	0.884484478249636	2.537467301800256
8	0.7305741788683847	2.395336345317663
9	0.6466231100600328	2.3850963629789024
>10	5.158993035524095	34.24372509451919
>50	0.05596737892081259	1.5814626579178495
>100	0.02098776709530472	1.438922123093863
>500	0.004997087403643981	1.3815782297738686
>1k	0.0019988349614575925	1.0948587631738957
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1453	0.5810094289073184	No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1220	0.48783998848377735	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	802	0.32069481210163064	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.31269743524124083	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC	626	0.2503178957302005	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	597	0.23872169928263529	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	566	0.22632576514903113	No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	412	0.16474596332402971	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	327	0.1307571116673731	No Hit
ACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	280	0.11196327604545708	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	266	0.10636511224318423	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3354899592933518	0.0
2	0.0	0.0	0.0	1.734631041018546	0.0
3	0.0	0.0	0.0	2.23286761942083	0.0
4	0.0	0.0	0.0	2.9410353404083462	0.0
5	0.0	0.0	0.0	4.859206180372838	0.0
6	0.0	0.0	0.0	5.845282747258899	0.0
7	0.0	0.0	0.0	6.880143312993338	0.0
8	0.0	0.0	0.0	8.637566878063994	0.0
9	0.0	0.0	0.0	9.248166601354756	0.0
10	0.0	0.0	0.0	10.883230300461449	0.0
11	0.0	0.0	0.0	12.804999960013117	0.0
12	0.0	0.0	0.0	14.581617229548709	0.0
13	0.0	0.0	0.0	15.298582065082654	0.0
14	0.0	0.0	0.0	15.590086451643861	0.0
15	0.0	0.0	0.0	16.156700602202477	0.0
16	0.0	0.0	0.0	17.239545429099255	0.0
17	0.0	0.0	0.0	18.47234107212834	0.0
18	0.0	0.0	0.0	19.880279268399963	0.0
19	0.0	0.0	0.0	20.69161315088651	0.0
20	0.0	0.0	0.0	21.438568149646915	0.0
21	0.0	0.0	0.0	22.357866619748723	0.0
22	0.0	0.0	0.0	23.266368631089	0.0
23	0.0	0.0	0.0	24.22565398549276	0.0
24	0.0	0.0	0.0	24.957413968218425	0.0
25	0.0	0.0	0.0	25.653185755072336	0.0
26	0.0	0.0	0.0	26.296974592333715	0.0
27	0.0	0.0	0.0	26.923169200502237	0.0
28	0.0	0.0	0.0	27.558560792060206	0.0
29	0.0	0.0	0.0	28.17355907262418	0.0
30	3.998688430194896E-4	0.0	0.0	28.84014043393767	0.0
31	3.998688430194896E-4	0.0	0.0	29.52711510624515	0.0
32	3.998688430194896E-4	0.0	0.0	30.143312993338185	0.0
33	3.998688430194896E-4	0.0	0.0	30.802296846634302	0.0
34	3.998688430194896E-4	0.0	0.0	31.445285946209644	0.0
35	3.998688430194896E-4	0.0	0.0	32.085076095040826	0.0
36	3.998688430194896E-4	0.0	0.0	32.73366335841844	0.0
37	3.998688430194896E-4	0.0	0.0	33.314272918482736	0.0
38	3.998688430194896E-4	0.0	0.0	33.934869362848985	0.0
39	7.997376860389792E-4	0.0	0.0	34.582257019697536	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACTC	20	7.0226885E-4	45.000004	8
CCGATGT	20	7.0226885E-4	45.000004	10
CCGATAG	20	7.0226885E-4	45.000004	11
TGGACCG	20	7.0226885E-4	45.000004	1
GGTCATC	20	7.0226885E-4	45.000004	8
TTGTACG	20	7.0226885E-4	45.000004	1
AGGCGGC	20	7.0226885E-4	45.000004	20
TGACGGA	20	7.0226885E-4	45.000004	18
TATGCGG	40	6.7775545E-9	45.000004	2
TATTGCG	20	7.0226885E-4	45.000004	1
GTTCGTG	20	7.0226885E-4	45.000004	1
CGTAGTT	20	7.0226885E-4	45.000004	26
CGGATAT	20	7.0226885E-4	45.000004	21
TGCGAAG	20	7.0226885E-4	45.000004	1
ACGGATA	20	7.0226885E-4	45.000004	20
GACGGAT	20	7.0226885E-4	45.000004	19
CGGTCCT	20	7.0226885E-4	45.000004	21
TGTACGG	40	6.7775545E-9	45.000004	2
GCGCTAG	20	7.0226885E-4	45.000004	1
TCGAAGG	20	7.0226885E-4	45.000004	2
>>END_MODULE