##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935492.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551988
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14184547490163	31.0	31.0	33.0	28.0	34.0
2	31.202783393841894	31.0	31.0	34.0	28.0	34.0
3	30.972917889519337	31.0	31.0	34.0	27.0	34.0
4	34.733294926701305	35.0	35.0	37.0	32.0	37.0
5	34.790826974499446	35.0	35.0	37.0	32.0	37.0
6	34.982827525236054	36.0	35.0	37.0	32.0	37.0
7	35.34651659094038	37.0	35.0	37.0	33.0	37.0
8	35.16005963897766	37.0	35.0	37.0	32.0	37.0
9	37.07979340130583	39.0	37.0	39.0	33.0	39.0
10	36.42659079545208	38.0	35.0	39.0	32.0	39.0
11	36.72281462640492	39.0	37.0	39.0	32.0	39.0
12	36.860815814836556	39.0	37.0	39.0	33.0	39.0
13	36.9103042095118	39.0	37.0	39.0	33.0	39.0
14	37.974742204540675	40.0	37.0	41.0	33.0	41.0
15	38.1151673587107	40.0	37.0	41.0	33.0	41.0
16	38.129761878881425	40.0	37.0	41.0	33.0	41.0
17	37.953683775734255	40.0	37.0	41.0	33.0	41.0
18	37.871542497300666	39.0	37.0	41.0	33.0	41.0
19	37.6508655985275	39.0	37.0	41.0	33.0	41.0
20	37.541457422987456	39.0	35.0	41.0	33.0	41.0
21	37.54451546048102	39.0	35.0	41.0	33.0	41.0
22	37.647648137278345	39.0	35.0	41.0	33.0	41.0
23	37.67231896345573	39.0	35.0	41.0	33.0	41.0
24	37.65048153220722	39.0	35.0	41.0	33.0	41.0
25	37.302653318550405	39.0	35.0	41.0	33.0	41.0
26	37.35551316332964	39.0	35.0	41.0	33.0	41.0
27	37.395988680913355	39.0	35.0	41.0	33.0	41.0
28	37.26756559925216	39.0	35.0	41.0	33.0	41.0
29	37.217107980608276	39.0	35.0	41.0	32.0	41.0
30	36.90672079827822	39.0	35.0	41.0	31.0	41.0
31	36.8016931527497	39.0	35.0	41.0	31.0	41.0
32	36.62670746465503	39.0	35.0	41.0	31.0	41.0
33	36.45185946071292	39.0	35.0	41.0	30.0	41.0
34	36.20395733240578	39.0	35.0	41.0	29.0	41.0
35	36.1257889664268	39.0	35.0	41.0	29.0	41.0
36	35.9163460075219	39.0	35.0	41.0	27.0	41.0
37	35.91756523692544	39.0	35.0	41.0	27.0	41.0
38	35.80025471568222	39.0	35.0	41.0	27.0	41.0
39	35.69691551265607	39.0	35.0	41.0	26.0	41.0
40	35.6724548359747	39.0	35.0	41.0	26.0	41.0
41	35.43266701450032	39.0	35.0	41.0	24.0	41.0
42	35.4881138720407	39.0	35.0	41.0	25.0	41.0
43	35.441371913882186	39.0	35.0	41.0	24.0	41.0
44	35.48177496612245	39.0	35.0	41.0	25.0	41.0
45	35.468343514714086	39.0	35.0	41.0	25.0	41.0
46	35.386200424646916	39.0	35.0	40.0	24.0	41.0
47	35.32250157612122	39.0	35.0	40.0	24.0	41.0
48	35.28092639695066	38.0	35.0	40.0	24.0	41.0
49	35.27640999442017	39.0	35.0	40.0	24.0	41.0
50	35.13749936592824	38.0	35.0	40.0	24.0	41.0
51	34.142965064457925	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	23.0
10	29.0
11	24.0
12	24.0
13	25.0
14	32.0
15	32.0
16	67.0
17	149.0
18	208.0
19	435.0
20	910.0
21	1542.0
22	2380.0
23	3408.0
24	4869.0
25	7013.0
26	9018.0
27	9268.0
28	9135.0
29	8974.0
30	9875.0
31	11953.0
32	15006.0
33	20887.0
34	34670.0
35	40073.0
36	42377.0
37	65077.0
38	116667.0
39	137803.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.740059566512315	18.41887867127546	21.61097705022573	17.230084711986493
2	37.4046174916846	24.394370892120843	22.320593925954913	15.880417690239643
3	29.737059501293505	24.55560628129597	30.590520083769935	15.116814133640585
4	25.307977709660356	22.948324963586163	33.727001311622715	18.016696015130766
5	25.46848844540099	27.611288651202564	28.804974021174374	18.115248882222076
6	22.64215888751205	33.89240345804619	29.69213098835482	13.773306666086944
7	72.52929411508946	5.1499307955970055	17.735168155829477	4.585606933484061
8	73.20068552214903	7.105045761864388	14.845974912498097	4.848293803488481
9	67.59404189946159	6.332927527410017	16.62373095067284	9.44929962245556
10	35.56961383218476	25.33696384704015	24.084762712232873	15.008659608542215
11	27.346790147611905	23.80051740255223	31.11516917034428	17.737523279491583
12	23.52587375087864	21.60137539221867	34.373935665268085	20.498815191634602
13	23.489641079153895	21.10444429951376	37.020913498119526	18.385001123212824
14	19.54209149474264	26.06976963267317	34.41904534156539	19.96909353101879
15	18.522141785690994	24.24110669072516	37.70444285020689	19.532308673376956
16	21.706087813503192	24.60216526446227	33.6246440140003	20.067102908034233
17	21.24991847648862	25.726283904722568	33.393660731755034	19.630136887033775
18	21.70445734327558	24.348355399030414	33.40163191953448	20.545555338159524
19	20.777082110480663	26.580650303992115	31.593621600469575	21.048645985057647
20	22.897418059812892	26.401660905671864	32.987854808437866	17.71306622607738
21	21.790691101980478	27.819988840337107	32.98767364507924	17.40164641260317
22	20.860779582164827	23.2881874243643	34.76669782676435	21.084335166706524
23	20.625086052595346	26.135713095212214	33.906171873301595	19.333028978890844
24	20.812046638695044	24.678616201801486	33.15814836554418	21.351188793959288
25	20.28015101777575	27.631216620651173	31.538910266165203	20.549722095407873
26	19.694993369421073	24.615209026283182	33.36087016384414	22.328927440451604
27	21.24756335282651	25.55834547127836	31.90739653760589	21.286694638289237
28	18.31126763625296	27.068523228765844	32.744371254447564	21.875837880533634
29	20.133046370573275	25.60472329108604	31.856851960549864	22.40537837779082
30	20.266382602520345	27.826691884606188	31.280933643485003	20.625991869388464
31	20.991760690449794	27.2895425262868	29.40770451531555	22.310992267947853
32	22.143053834503647	28.714935831938377	29.008782799626076	20.133227533931898
33	20.9620498996355	27.388095393378116	28.743197315883677	22.906657391102705
34	19.69861663659355	27.838467502916732	30.273303042819773	22.18961281766995
35	20.261310028478878	29.22635999333319	29.038131263723127	21.474198714464805
36	19.871446480720596	30.06550867047834	28.957513569135564	21.1055312796655
37	19.80332905787807	30.483814865540555	29.49375711066183	20.21909896591955
38	20.27054935976869	29.022370051522863	28.38938527649152	22.31769531221693
39	21.06676232092002	28.156771524018637	29.100270295731068	21.676195859330274
40	21.96768770335587	25.357435306564636	30.52856221512062	22.146314774958874
41	19.55767154358428	27.57541830619506	30.142140771176184	22.72476937904447
42	21.348471343579934	26.363254273643633	30.708638593592614	21.579635789183822
43	20.627260012898834	25.785524322992533	30.753385943172677	22.833829720935963
44	20.657695457147618	26.00708711058936	29.93507105226925	23.40014637999377
45	20.447364797785458	25.375008152351135	29.61115096705001	24.566476082813395
46	22.365884765610847	26.324122988180903	28.960049856156296	22.349942390051957
47	19.46564055740342	26.309992246208253	32.02189178025609	22.202475416132234
48	19.679594483938057	26.536989934563792	30.820054059146212	22.963361522351935
49	20.995927447698136	24.68169597889809	32.17732994195526	22.14504663144851
50	19.75785705486351	24.71213142314688	31.99272447951767	23.537287042471938
51	19.424516474995833	24.079146648115536	30.73110285006196	25.765234026826672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	448.0
2	694.0
3	7256.0
4	13818.0
5	8922.0
6	4026.0
7	3949.0
8	3872.0
9	3795.0
10	3718.0
11	3686.0
12	3654.0
13	3496.5
14	3339.0
15	3137.5
16	2936.0
17	2859.0
18	2782.0
19	2745.5
20	2709.0
21	2819.0
22	2929.0
23	3010.0
24	3091.0
25	3503.0
26	4437.5
27	4960.0
28	5835.0
29	6710.0
30	7877.0
31	9044.0
32	9862.0
33	10680.0
34	12628.0
35	14576.0
36	16237.0
37	17898.0
38	19355.5
39	20813.0
40	22715.5
41	24618.0
42	27927.5
43	31237.0
44	44759.5
45	58282.0
46	54326.5
47	50371.0
48	50954.0
49	51537.0
50	46179.5
51	40822.0
52	37233.5
53	33645.0
54	30915.0
55	28185.0
56	25994.5
57	23804.0
58	21909.0
59	20014.0
60	18312.0
61	16610.0
62	14792.0
63	12974.0
64	10959.0
65	8944.0
66	7317.5
67	5691.0
68	4640.0
69	3589.0
70	2844.5
71	2100.0
72	1769.5
73	1439.0
74	1161.0
75	672.0
76	461.0
77	322.0
78	183.0
79	146.5
80	110.0
81	91.5
82	73.0
83	47.5
84	22.0
85	20.0
86	18.0
87	10.0
88	2.0
89	2.0
90	2.0
91	3.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	551988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.26767144034588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80387071084628	29.316423453697787
2	8.854364504130654	7.130892813307886
3	4.07797644771749	4.926318472144701
4	2.5753748420022116	4.14817391893911
5	1.9144567828902805	3.85453583600837
6	1.5936155944197377	3.85027134949833
7	1.3254197583327516	3.736009714436012
8	1.1165821494642134	3.5969730504624096
9	0.9588059142800578	3.4747993378160826
>10	4.722257823434816	27.841026895852817
>50	0.033637975903036375	0.9479830294430552
>100	0.015455286224373732	0.99118132923331
>500	0.004545672418933451	1.2388393788090568
>1k	0.0027274034513600706	2.330145581004624
>5k	9.091344837866901E-4	2.616425839346433
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9064	1.6420646825655631	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	5230	0.9474843656021508	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC	3884	0.7036384848945991	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG	3408	0.6174047261896998	No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1638	0.29674558142568314	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	1449	0.26250570664579664	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT	1306	0.23659934636260208	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG	1045	0.18931570976180642	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	957	0.17337333420291745	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT	827	0.1498220975818315	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT	746	0.1351478655333087	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC	659	0.11938665333304348	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAACAGTT	649	0.11757501974680609	No Hit
CGTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	643	0.11648803959506365	No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	609	0.11032848540185657	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	597	0.1081545250983717	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTA	580	0.10507474800176815	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.811633586237382E-4	0.0	0.0	0.1143140792915788	0.0
2	1.811633586237382E-4	0.0	0.0	0.5969332666652174	0.0
3	1.811633586237382E-4	0.0	0.0	0.9598034739885649	0.0
4	1.811633586237382E-4	0.0	0.0	1.5985854764958658	0.0
5	1.811633586237382E-4	0.0	0.0	3.427973071878374	0.0
6	1.811633586237382E-4	0.0	0.0	4.652456212816221	0.0
7	1.811633586237382E-4	0.0	0.0	5.649760502039899	0.0
8	1.811633586237382E-4	0.0	0.0	6.995985419972898	0.0
9	1.811633586237382E-4	0.0	0.0	7.682232222439619	0.0
10	3.623267172474764E-4	0.0	0.0	9.249838764610825	0.0
11	3.623267172474764E-4	0.0	0.0	10.258737508786423	0.0
12	3.623267172474764E-4	0.0	0.0	11.801343507467553	3.623267172474764E-4
13	3.623267172474764E-4	0.0	0.0	12.294107842924122	3.623267172474764E-4
14	3.623267172474764E-4	0.0	0.0	12.556070059494047	3.623267172474764E-4
15	3.623267172474764E-4	0.0	0.0	12.921657717196751	3.623267172474764E-4
16	3.623267172474764E-4	0.0	0.0	13.368587722921513	3.623267172474764E-4
17	3.623267172474764E-4	0.0	0.0	13.87439582019899	3.623267172474764E-4
18	3.623267172474764E-4	0.0	0.0	14.414624955614977	3.623267172474764E-4
19	3.623267172474764E-4	0.0	0.0	14.901954390312833	3.623267172474764E-4
20	3.623267172474764E-4	0.0	0.0	15.262469473974072	3.623267172474764E-4
21	3.623267172474764E-4	0.0	0.0	15.679688688884541	3.623267172474764E-4
22	3.623267172474764E-4	0.0	0.0	16.15596715870635	3.623267172474764E-4
23	3.623267172474764E-4	0.0	0.0	16.578802437734154	3.623267172474764E-4
24	3.623267172474764E-4	0.0	0.0	16.951998956499054	3.623267172474764E-4
25	3.623267172474764E-4	0.0	0.0	17.298926788263515	3.623267172474764E-4
26	3.623267172474764E-4	0.0	0.0	17.636434125379537	3.623267172474764E-4
27	3.623267172474764E-4	0.0	0.0	17.998760842627014	3.623267172474764E-4
28	3.623267172474764E-4	0.0	0.0	18.34460169423973	3.623267172474764E-4
29	5.434900758712146E-4	0.0	0.0	18.712725638963168	3.623267172474764E-4
30	5.434900758712146E-4	0.0	0.0	19.1563947042327	3.623267172474764E-4
31	5.434900758712146E-4	0.0	0.0	19.551149662673826	3.623267172474764E-4
32	5.434900758712146E-4	0.0	0.0	19.945723457756326	3.623267172474764E-4
33	5.434900758712146E-4	0.0	0.0	20.323630223845445	3.623267172474764E-4
34	5.434900758712146E-4	0.0	0.0	20.71874750900382	3.623267172474764E-4
35	5.434900758712146E-4	0.0	0.0	21.125096922396864	3.623267172474764E-4
36	5.434900758712146E-4	0.0	0.0	21.50626462894121	3.623267172474764E-4
37	5.434900758712146E-4	0.0	0.0	21.903918201120316	3.623267172474764E-4
38	7.246534344949528E-4	0.0	0.0	22.36226149843837	3.623267172474764E-4
39	7.246534344949528E-4	0.0	0.0	22.885642441502352	3.623267172474764E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	20	7.0300983E-4	45.0	33
TGGACGA	20	7.0300983E-4	45.0	5
GTTTACG	20	7.0300983E-4	45.0	1
CACTCGT	20	7.0300983E-4	45.0	38
TCGTGCG	35	1.2103919E-7	45.0	1
CGGATTG	20	7.0300983E-4	45.0	2
GCGATAC	25	3.8881622E-5	45.0	9
ATCGAAT	55	1.8189894E-12	45.0	43
CCCCTCG	30	2.1634442E-6	44.999996	40
CGTTTTT	4030	0.0	43.492558	1
GGCGAAC	115	0.0	43.043476	32
CCGATGA	585	0.0	41.923073	18
CGATGAA	615	0.0	40.609756	19
CGGCGAA	150	0.0	40.5	31
TACGGGT	50	1.0786607E-9	40.5	4
GCGTAAG	50	1.0786607E-9	40.5	1
CCGATCG	45	1.9252184E-8	40.0	40
GCGCGAC	45	1.9252184E-8	40.0	9
CGCGACC	45	1.9252184E-8	40.0	10
GGCGCGA	45	1.9252184E-8	40.0	8
>>END_MODULE