##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935491.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211024
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.980675183865344	31.0	30.0	33.0	28.0	34.0
2	30.95470657365987	31.0	30.0	33.0	28.0	34.0
3	30.80955246796573	31.0	30.0	34.0	27.0	34.0
4	34.69451342027447	35.0	35.0	37.0	32.0	37.0
5	34.66191049359315	35.0	35.0	37.0	32.0	37.0
6	34.844742778072636	35.0	35.0	37.0	32.0	37.0
7	35.210838577602544	36.0	35.0	37.0	33.0	37.0
8	34.820195807111986	37.0	35.0	37.0	32.0	37.0
9	36.5476106983092	39.0	35.0	39.0	32.0	39.0
10	36.03540355599363	37.0	35.0	39.0	32.0	39.0
11	36.45130411706725	38.0	35.0	39.0	32.0	39.0
12	36.569845136098266	38.0	35.0	39.0	32.0	39.0
13	36.46442584729699	39.0	35.0	39.0	32.0	39.0
14	37.47122128288726	39.0	36.0	40.0	32.0	41.0
15	37.58383880506483	39.0	36.0	41.0	32.0	41.0
16	37.71753449844567	39.0	36.0	41.0	33.0	41.0
17	37.49663545378725	39.0	36.0	40.0	32.0	41.0
18	37.54408977177951	39.0	36.0	40.0	33.0	41.0
19	37.466169724770644	39.0	36.0	40.0	32.0	41.0
20	37.28932728030935	39.0	35.0	40.0	32.0	41.0
21	37.419464136780654	39.0	36.0	40.0	32.0	41.0
22	37.54118015012511	39.0	36.0	41.0	32.0	41.0
23	37.50387159754341	39.0	36.0	41.0	32.0	41.0
24	37.49335620592918	39.0	35.0	41.0	32.0	41.0
25	37.22348642808401	39.0	35.0	40.0	32.0	41.0
26	37.20209075744939	39.0	35.0	41.0	32.0	41.0
27	37.18228258397149	39.0	35.0	41.0	32.0	41.0
28	37.05738209871863	39.0	35.0	41.0	31.0	41.0
29	37.0477196906513	39.0	35.0	41.0	31.0	41.0
30	36.853286829934035	39.0	35.0	41.0	31.0	41.0
31	36.90078379710365	39.0	35.0	41.0	31.0	41.0
32	36.78700053074532	39.0	35.0	41.0	31.0	41.0
33	36.794729509439684	39.0	35.0	41.0	31.0	41.0
34	36.700882364091285	39.0	35.0	41.0	31.0	41.0
35	36.71793729623171	39.0	35.0	41.0	31.0	41.0
36	36.547549093941925	39.0	35.0	41.0	30.0	41.0
37	36.58385776025476	39.0	35.0	41.0	30.0	41.0
38	36.42022234437789	39.0	35.0	41.0	30.0	41.0
39	36.27449010539085	39.0	35.0	41.0	30.0	41.0
40	36.33577223443779	39.0	35.0	41.0	30.0	41.0
41	36.009719273637124	39.0	35.0	40.0	28.0	41.0
42	36.20141784820684	39.0	35.0	41.0	30.0	41.0
43	36.18786014860869	39.0	35.0	40.0	30.0	41.0
44	36.27541890969748	39.0	35.0	41.0	30.0	41.0
45	36.25170596709379	39.0	35.0	40.0	30.0	41.0
46	36.136794866934565	39.0	35.0	40.0	29.0	41.0
47	35.90591591477747	39.0	35.0	40.0	28.0	41.0
48	35.941603798620065	39.0	35.0	40.0	29.0	41.0
49	35.98038137842141	39.0	35.0	40.0	29.0	41.0
50	35.84570949275912	39.0	35.0	40.0	28.0	41.0
51	34.62951133520358	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	13.0
11	13.0
12	1.0
13	7.0
14	5.0
15	7.0
16	20.0
17	37.0
18	75.0
19	152.0
20	326.0
21	654.0
22	1167.0
23	1543.0
24	1761.0
25	1984.0
26	2140.0
27	2118.0
28	2365.0
29	2830.0
30	3769.0
31	4947.0
32	6537.0
33	9084.0
34	14290.0
35	16774.0
36	17665.0
37	26426.0
38	46106.0
39	48188.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.50822655243006	18.76469027219653	22.323053301994086	10.404029873379331
2	28.253658351656686	33.10334369550383	22.970846917886117	15.672151034953371
3	28.470221396618395	32.203446053529454	25.21277200697551	14.113560542876641
4	25.851561907650318	21.884714534839638	35.24338463871408	17.020338918795964
5	30.796497080900753	26.109826370460233	24.043710667980893	19.049965880658124
6	21.339278944575025	37.83124194404428	26.057699598149973	14.771779513230724
7	69.69539009780878	5.351050117522178	19.858878610963682	5.094681173705361
8	68.49363105618318	12.939760406399273	11.572617332625674	6.993991204791872
9	62.9677193115475	6.866517552505876	13.336397755705512	16.82936538024111
10	37.772954735006444	25.09477594965502	21.47433467283342	15.657934642505117
11	30.203673515808628	22.491754492380014	27.506823868375164	19.797748123436197
12	24.541758283417998	18.751895518993102	32.44844188338767	24.257904314201227
13	24.24605732049435	23.579782394419592	35.840473121540676	16.33368716354538
14	18.66517552505876	31.55754795663053	28.504814618242474	21.272461900068237
15	15.100652058533626	23.945617560087953	39.49977253772083	21.45395784365759
16	17.265334748654183	25.31702555159603	29.841629388126467	27.57601031162332
17	17.671449692925922	26.552903935097426	30.22073318674653	25.554913185230117
18	18.50358253089696	24.10578891500493	31.721036469785428	25.669592084312686
19	18.817764804003335	26.23066570627038	28.11338994616726	26.838179543559026
20	21.43689817271969	25.838293274698614	33.42226476609296	19.30254378648874
21	19.859826370460233	30.95619455606945	30.923496853438472	18.260482220031847
22	17.080995526575176	23.010178936992947	33.00998938509364	26.898836151338234
23	18.558078701948595	28.131397376601715	30.470941693835773	22.83958222761392
24	20.744085980741527	25.62362574873	29.243119266055047	24.389169004473423
25	16.961103950261585	33.57817120327546	26.288479035559938	23.172245810903025
26	15.94273637121844	24.893850936386382	31.21256349988627	27.950849192508908
27	21.732599135643337	25.807964970809007	27.307320494351355	25.1521153991963
28	14.681268481310184	26.23066570627038	34.446318902115394	24.64174691030404
29	17.429771021305633	23.070361665023885	30.38327394040488	29.116593373265598
30	17.48000227462279	26.670899992417922	28.920407157479715	26.928690575479564
31	21.4842861475472	23.659394192129803	27.494029115171735	27.362290545151264
32	21.161100159223594	30.01459549624687	24.062665857911895	24.761638486617635
33	20.05932974448404	24.41096747289408	24.41096747289408	31.118735309727803
34	22.132553643187507	22.53345591022822	27.14714913943438	28.186841307149894
35	18.7424179240276	23.41060732428539	24.170710440518615	33.676264311168396
36	18.914436272651454	26.8196982333763	26.820172113124574	27.445693380847676
37	17.64206914853287	26.0325839714914	30.00606566077792	26.319281219197816
38	16.170672530138752	26.105561452725755	27.249981044810067	30.473784972325426
39	22.16762074455986	27.302581696868604	26.193703085904918	24.336094472666616
40	20.284896504662978	21.417942982788688	27.593069982561225	30.70409052998711
41	19.947494123891122	25.55823034346804	24.599097732959287	29.895177799681555
42	18.051975130790808	23.256122526347713	29.597581317764803	29.09432102509667
43	20.493877473652287	25.52695428008189	26.65668359996967	27.322484646296157
44	19.252786412919857	22.58558268253848	29.908920312381532	28.252710592160135
45	18.824873000227463	21.471965274092046	26.85429145500038	32.848870270680116
46	25.215141405716885	25.171070589127304	25.598036242323147	24.015751762832664
47	16.58057851239669	22.823470316172568	34.68420653574949	25.91174463568125
48	19.021059216013345	24.522329213738722	27.812950185760858	28.643661384487075
49	18.713037379634546	20.414739555690346	34.98227689741451	25.889946167260597
50	20.903783455910226	21.0298354689514	30.353419516263553	27.71296155887482
51	18.329668663280003	20.38109409356282	27.28030934869967	34.0089278944575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	176.0
2	244.0
3	726.0
4	1208.0
5	794.0
6	380.0
7	387.0
8	394.0
9	402.0
10	410.0
11	413.0
12	416.0
13	410.0
14	404.0
15	376.0
16	348.0
17	355.0
18	362.0
19	351.5
20	341.0
21	402.0
22	463.0
23	516.5
24	570.0
25	860.5
26	1205.0
27	1259.0
28	1614.5
29	1970.0
30	2416.0
31	2862.0
32	3414.0
33	3966.0
34	4551.0
35	5136.0
36	5502.0
37	5868.0
38	6433.5
39	6999.0
40	7782.0
41	8565.0
42	9570.0
43	10575.0
44	11743.5
45	12912.0
46	13867.5
47	14823.0
48	26334.0
49	37845.0
50	31265.0
51	24685.0
52	23764.5
53	22844.0
54	18657.5
55	14471.0
56	12081.0
57	9691.0
58	8272.0
59	6853.0
60	5674.0
61	4495.0
62	3739.5
63	2984.0
64	2519.5
65	2055.0
66	1734.5
67	1414.0
68	1131.0
69	848.0
70	662.0
71	476.0
72	384.0
73	292.0
74	219.0
75	127.0
76	108.0
77	78.5
78	49.0
79	37.5
80	26.0
81	14.5
82	3.0
83	3.0
84	3.0
85	1.5
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	211024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.79926453863068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3042173695616	29.97052467965729
2	10.512466363441526	8.367768595041323
3	3.722048912914057	4.444044279323679
4	1.8205415188245662	2.8982485404503753
5	1.2287762246088634	2.445219501099401
6	0.9382516133641321	2.2405034498445673
7	0.7263115281118282	2.023466525134582
8	0.6501083513918987	2.0699067404655396
9	0.5941466434882003	2.128193949503374
>10	4.404305479484676	28.755970884828265
>50	0.060724406448693834	1.647679884752445
>100	0.022622818088729076	1.6808514671317005
>500	0.00952539708999119	3.1413488513154904
>1k	0.004762698544995595	5.786071726438699
>5k	0.0011906746362488987	2.4002009250132685
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	5065	2.4002009250132685	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC	4886	2.31537645007203	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	3913	1.854291455000379	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2331	1.104613693229206	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT	1080	0.5117901281370839	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	981	0.46487603305785125	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT	954	0.4520812798544241	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTACGCAT	887	0.4203313367199939	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTA	836	0.396163469557965	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.39379407081658957	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTC	773	0.36630904541663506	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	729	0.34545833649253166	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	638	0.3023352793994996	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	394	0.18670862082038062	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	262	0.12415649404807036	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	252	0.11941769656531957	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATG	237	0.11230950034119341	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCTACGCATCGT	224	0.10614906361361741	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.738797482750777E-4	0.0	0.0	0.31607779209947684	0.0
2	4.738797482750777E-4	0.0	0.0	2.1514140571688527	0.0
3	4.738797482750777E-4	0.0	0.0	2.665099704299037	0.0
4	4.738797482750777E-4	0.0	0.0	3.7834559102282204	0.0
5	4.738797482750777E-4	0.0	0.0	7.8341799984835845	0.0
6	4.738797482750777E-4	0.0	0.0	9.251080445826068	0.0
7	4.738797482750777E-4	0.0	0.0	10.61111532337554	0.0
8	4.738797482750777E-4	0.0	0.0	12.434604594738039	0.0
9	4.738797482750777E-4	0.0	0.0	12.919383577223444	0.0
10	4.738797482750777E-4	0.0	0.0	15.974960194101145	0.0
11	4.738797482750777E-4	0.0	0.0	17.78518083251194	0.0
12	4.738797482750777E-4	0.0	0.0	21.731177496398512	0.0
13	4.738797482750777E-4	0.0	0.0	22.543407384941997	0.0
14	4.738797482750777E-4	0.0	0.0	22.918720145575858	0.0
15	4.738797482750777E-4	0.0	0.0	23.818143907801957	0.0
16	4.738797482750777E-4	0.0	0.0	24.85641443627265	0.0
17	4.738797482750777E-4	0.0	0.0	26.113617408446434	0.0
18	4.738797482750777E-4	0.0	0.0	27.442376222609752	0.0
19	4.738797482750777E-4	0.0	0.0	28.645556903480173	0.0
20	4.738797482750777E-4	0.0	0.0	29.39191750701342	0.0
21	4.738797482750777E-4	0.0	0.0	30.23352793994996	0.0
22	4.738797482750777E-4	0.0	0.0	31.165649404807038	0.0
23	4.738797482750777E-4	0.0	0.0	32.025741147926304	0.0
24	4.738797482750777E-4	0.0	0.0	32.683012358783834	0.0
25	4.738797482750777E-4	0.0	0.0	33.27157100614148	0.0
26	4.738797482750777E-4	0.0	0.0	33.918416862536965	0.0
27	4.738797482750777E-4	0.0	0.0	34.623076048222	0.0
28	4.738797482750777E-4	0.0	0.0	35.2391197209796	0.0
29	4.738797482750777E-4	0.0	0.0	35.81014481765107	0.0
30	4.738797482750777E-4	0.0	0.0	36.47547198422928	0.0
31	9.477594965501554E-4	0.0	0.0	37.05597467586625	0.0
32	9.477594965501554E-4	0.0	0.0	37.63079081052392	0.0
33	9.477594965501554E-4	0.0	0.0	38.20371142618849	0.0
34	0.0014216392448252332	0.0	0.0	38.77426264311168	0.0
35	0.0014216392448252332	0.0	0.0	39.394097353855486	0.0
36	0.0014216392448252332	0.0	0.0	39.9584881340511	0.0
37	0.0018955189931003109	0.0	0.0	40.491602850860566	0.0
38	0.0018955189931003109	0.0	0.0	41.05741527030101	0.0
39	0.0018955189931003109	0.0	0.0	41.68009325953446	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCT	20	7.0201844E-4	45.000004	11
GTCTGCG	20	7.0201844E-4	45.000004	1
ACTGACG	20	7.0201844E-4	45.000004	43
TTAGCGG	20	7.0201844E-4	45.000004	2
GCGCGAC	20	7.0201844E-4	45.000004	9
CGGTTGG	20	7.0201844E-4	45.000004	2
CGACCTC	20	7.0201844E-4	45.000004	12
ACGGGTG	20	7.0201844E-4	45.000004	5
TGGCCGG	20	7.0201844E-4	45.000004	2
AGTTGCG	20	7.0201844E-4	45.000004	1
TAGGGCG	20	7.0201844E-4	45.000004	5
TAGGCTA	20	7.0201844E-4	45.000004	24
TAAGCAA	20	7.0201844E-4	45.000004	19
ACGAGAG	20	7.0201844E-4	45.000004	3
AAGTGCG	20	7.0201844E-4	45.000004	1
AGAGCGG	20	7.0201844E-4	45.000004	2
TTGGGCA	20	7.0201844E-4	45.000004	5
GGCTAAC	20	7.0201844E-4	45.000004	9
CGCGGGA	70	0.0	45.0	4
TCGGGAC	25	3.8799506E-5	45.0	5
>>END_MODULE