FastQCFastQC Report
Sat 14 Jan 2017
SRR2935488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653749
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124111.898435026286847No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT29110.44527792776738473No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC23120.35365254860810497TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG20700.3166352835721355TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC18090.2767117043391271No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT17650.26998129251440534No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC13040.19946493225993464Illumina PCR Primer Index 7 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT12360.18906338671263742TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC11870.18156815536237914Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT10170.15556429149413611TruSeq Adapter, Index 23 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC8530.13047821105653698No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC7740.11839406255305936No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC6870.10508620280872323No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG253.8889524E-545.0000041
TCGCAAG302.164059E-645.0000041
GCGTAAG502.1827873E-1145.0000041
GTACGAG302.164059E-645.0000041
CGACGGT453.8380676E-1045.028
ATATGCG207.031052E-445.01
CGGTCTA453.8380676E-1045.031
CGTTTTT58300.044.1509441
ATACGGA501.0804797E-940.5000044
CGTTTCT2850.039.4736861
CGACAGG800.039.3752
GCGATAT403.4551704E-739.3759
TAGCTAG800.039.3751
TTACGGG1150.039.1304363
CCGATAC356.244156E-638.5714311
TGTTGCG356.244156E-638.571431
ATGCGCG356.244156E-638.571431
CGCCCAA700.038.5714337
CGTGCGG1050.038.5714262
GTTTTTT67750.038.191882
Produced by FastQC (version 0.11.5)