##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935488.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	653749
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22967377387958	31.0	31.0	33.0	28.0	34.0
2	31.291821478885627	31.0	31.0	34.0	28.0	34.0
3	31.022184355157712	31.0	31.0	34.0	27.0	34.0
4	34.75837821549249	35.0	35.0	37.0	32.0	37.0
5	34.85166478266124	35.0	35.0	37.0	33.0	37.0
6	35.07864639181093	37.0	35.0	37.0	32.0	37.0
7	35.39957231292132	37.0	35.0	37.0	33.0	37.0
8	35.27600807037563	37.0	35.0	37.0	32.0	37.0
9	37.24036901012468	39.0	37.0	39.0	34.0	39.0
10	36.63040402356256	38.0	35.0	39.0	32.0	39.0
11	36.896426610212785	39.0	37.0	39.0	33.0	39.0
12	37.033044792420334	39.0	37.0	39.0	33.0	39.0
13	37.11790916697387	39.0	37.0	39.0	33.0	39.0
14	38.16539681131444	40.0	38.0	41.0	33.0	41.0
15	38.3527179391479	40.0	38.0	41.0	34.0	41.0
16	38.293728938782316	40.0	38.0	41.0	34.0	41.0
17	38.21224506653165	40.0	37.0	41.0	34.0	41.0
18	38.069569513681856	39.0	37.0	41.0	34.0	41.0
19	37.837004721995754	39.0	37.0	41.0	34.0	41.0
20	37.81104062874284	39.0	35.0	41.0	34.0	41.0
21	37.73791929318439	39.0	35.0	41.0	33.0	41.0
22	37.85541239833636	39.0	35.0	41.0	34.0	41.0
23	37.90883504219509	40.0	35.0	41.0	34.0	41.0
24	37.88301626465203	40.0	35.0	41.0	34.0	41.0
25	37.46629516832913	39.0	35.0	41.0	33.0	41.0
26	37.54934080205094	39.0	35.0	41.0	33.0	41.0
27	37.6419390316467	39.0	35.0	41.0	33.0	41.0
28	37.52774229864979	39.0	35.0	41.0	33.0	41.0
29	37.445935672559344	39.0	35.0	41.0	33.0	41.0
30	37.0894487027896	39.0	35.0	41.0	32.0	41.0
31	36.973583133587965	39.0	35.0	41.0	32.0	41.0
32	36.796769096396325	39.0	35.0	41.0	31.0	41.0
33	36.571122862138225	39.0	35.0	41.0	31.0	41.0
34	36.314198568563775	39.0	35.0	41.0	30.0	41.0
35	36.20886609386783	39.0	35.0	41.0	29.0	41.0
36	36.03459584641812	39.0	35.0	41.0	28.0	41.0
37	36.021190089774514	39.0	35.0	41.0	28.0	41.0
38	35.87850077017326	39.0	35.0	41.0	27.0	41.0
39	35.78459469918883	39.0	35.0	41.0	26.0	41.0
40	35.70744735364796	39.0	35.0	41.0	26.0	41.0
41	35.525743060410036	39.0	35.0	41.0	24.0	41.0
42	35.541118992151425	39.0	35.0	41.0	24.0	41.0
43	35.52903025473079	39.0	35.0	41.0	24.0	41.0
44	35.494968252341494	39.0	35.0	41.0	24.0	41.0
45	35.4552787078833	39.0	35.0	41.0	24.0	41.0
46	35.36713019828711	39.0	35.0	41.0	24.0	41.0
47	35.35924490897883	39.0	35.0	41.0	24.0	41.0
48	35.262050114034594	39.0	35.0	40.0	23.0	41.0
49	35.24085237606482	39.0	35.0	41.0	24.0	41.0
50	35.1448201067994	39.0	35.0	40.0	24.0	41.0
51	34.07044599685812	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	19.0
9	49.0
10	32.0
11	32.0
12	33.0
13	20.0
14	37.0
15	40.0
16	73.0
17	148.0
18	276.0
19	532.0
20	809.0
21	1355.0
22	2005.0
23	3081.0
24	5189.0
25	8403.0
26	11245.0
27	11848.0
28	11139.0
29	10467.0
30	11138.0
31	13035.0
32	16808.0
33	23003.0
34	38077.0
35	45226.0
36	49945.0
37	77241.0
38	142034.0
39	170394.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.899332924409826	19.66840484650837	22.058159935999903	19.3741022930819
2	40.656582266282626	21.179994156778825	22.426955911213632	15.736467665724918
3	30.098707607965746	21.319803166046906	33.32609304182492	15.255396184162423
4	26.56937142542474	23.577856333241044	32.17886375352008	17.67390848781413
5	22.8447003360617	27.69656244216052	31.333432249991972	18.12530497178581
6	22.840417346718695	31.64119562706788	31.17526757211101	14.343119454102416
7	72.79093352341648	5.322532042114022	16.93295133147431	4.953583102995187
8	74.12921472920036	4.566737386978795	16.381057561847133	4.922990321973724
9	67.50679542148438	6.9028021457776605	18.85065980980468	6.739742622933266
10	33.436227053502186	26.918129129069413	25.280803488800746	14.364840328627654
11	25.524016097921375	23.671317279261615	32.922421296246725	17.88224532657029
12	23.105962685984988	21.15307250947994	36.27584898791432	19.465115816620752
13	22.748639003654308	20.7192668745956	38.283653206352895	18.248440915397193
14	19.380679741001515	24.190323809290724	37.218259607280466	19.21073684242729
15	18.648747455063027	24.4931923414032	38.55883527164095	18.299224931892823
16	21.71016705188077	24.278125090822318	35.97374527532738	18.037962581969534
17	21.550625698853842	24.348335523266577	33.939325337400135	20.16171344047945
18	21.52737518527753	24.27843101863253	34.949804894539035	19.244388901550902
19	20.46106380277446	26.724017933488238	33.126016253944556	19.68890200979275
20	21.46297738122735	26.338242964807595	32.993396548216516	19.205383105748535
21	21.44599838776044	26.17961939521131	34.36028200425545	18.014100212772792
22	20.215403771172117	23.53777979010293	35.13152601380652	21.11529042491843
23	19.26595681217103	25.352237632485863	34.75615259067318	20.62565296466993
24	20.375862907629685	24.633460242386604	34.30261461203	20.688062237953712
25	21.31506128498858	25.69502974383135	32.36257340355396	20.62733556762611
26	19.89876848759998	25.89357689266064	32.738099790592415	21.46955482914697
27	20.47115942051154	25.44355708383493	33.145595633798294	20.939687861855237
28	19.86129233084869	25.462524608068232	33.5766479183907	21.09953514269238
29	20.363472831315995	26.006464254629837	32.35400742486795	21.276055489186216
30	21.12324454798401	26.09380664444611	32.62291797004661	20.16003083752327
31	21.811735084872023	26.24937093594025	31.615344727104745	20.323549252082987
32	22.125158126436904	26.203175836597836	31.898174987648165	19.773491049317094
33	20.79911403306162	25.63522085693439	32.15882548195103	21.406839628052968
34	19.965001858511446	25.03728495186991	33.57909534087241	21.418617848746234
35	18.979608381810145	26.167994138423158	33.52922910780743	21.32316837195927
36	20.548865084306055	26.61373095790586	31.34123340915244	21.496170548635636
37	19.948634720664966	26.3803080387121	32.29496335749653	21.376093883126398
38	19.890661400629295	25.6312437954016	32.2753839776428	22.20271082632631
39	20.426035068504884	23.95827756524293	31.722266496774754	23.89342086947743
40	21.342288860097682	23.75407075192467	33.49282369839189	21.410816689585758
41	19.473222903591438	25.01908224716214	32.466282931216725	23.041411918029702
42	20.06672285540781	26.012123919118807	31.963949466844312	21.957203758629078
43	20.714524993537275	24.944435861469767	31.975421759727357	22.365617385265598
44	21.384048006191982	24.57625174187647	31.141615512987403	22.898084738944153
45	20.60270837890383	24.09839250232123	31.052590520214945	24.24630859856
46	21.473837818489972	24.70198807187468	30.390256811100286	23.433917298535064
47	20.189246943398768	24.848068601252162	32.42865381056032	22.53403064478875
48	19.576626503443983	24.7526191244652	32.60364451800309	23.067109854087732
49	20.83720204543334	23.38634552404669	32.41412223957513	23.362330190944842
50	19.422285923190703	23.55261728889834	33.58980281422993	23.43529397368103
51	19.529207692860716	22.67383965405683	32.67767904807503	25.119273605007425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	263.0
1	466.5
2	670.0
3	9596.0
4	18522.0
5	12002.0
6	5482.0
7	5309.0
8	5136.0
9	5181.0
10	5226.0
11	5130.5
12	5035.0
13	4891.0
14	4747.0
15	4601.5
16	4456.0
17	4242.5
18	4029.0
19	3803.5
20	3578.0
21	3545.0
22	3512.0
23	3663.0
24	3814.0
25	3923.5
26	4911.0
27	5789.0
28	6480.5
29	7172.0
30	8344.5
31	9517.0
32	11083.0
33	12649.0
34	14297.0
35	15945.0
36	18037.0
37	20129.0
38	21941.5
39	23754.0
40	27149.0
41	30544.0
42	33979.0
43	37414.0
44	48076.0
45	58738.0
46	61912.0
47	65086.0
48	65393.5
49	65701.0
50	58081.0
51	50461.0
52	44610.0
53	38759.0
54	34781.0
55	30803.0
56	28830.5
57	26858.0
58	25024.0
59	23190.0
60	21523.0
61	19856.0
62	17549.5
63	15243.0
64	12929.0
65	10615.0
66	8668.0
67	6721.0
68	5368.5
69	4016.0
70	3307.5
71	2599.0
72	2110.5
73	1622.0
74	1296.5
75	738.5
76	506.0
77	436.5
78	367.0
79	238.0
80	109.0
81	84.5
82	60.0
83	46.5
84	33.0
85	20.0
86	7.0
87	5.5
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	653749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.54642330433658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8559854292496	31.42308019631768
2	9.363352036342318	7.967542785714844
3	4.15661415222028	5.305471956994804
4	2.5167404114704044	4.283132115742001
5	1.883262058347703	4.006303236372879
6	1.3965540265655905	3.5651027268981195
7	1.0858286042026388	3.2338686401253693
8	0.9146889793019449	3.113339560415368
9	0.7356225675780974	2.8168298237160605
>10	4.033359868969845	26.897785393303568
>50	0.03189552693897284	0.9078293033161761
>100	0.019934703259591558	1.4378461117670622
>500	0.0025371440512207315	0.7206193565301867
>1k	0.003262042351569512	2.4073590359068575
>5k	0.0	0.0
>10k+	3.624491501743902E-4	1.9138897568791242
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12411	1.898435026286847	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2911	0.44527792776738473	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	2312	0.35365254860810497	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	2070	0.3166352835721355	TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	1809	0.2767117043391271	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	1765	0.26998129251440534	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1304	0.19946493225993464	Illumina PCR Primer Index 7 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1236	0.18906338671263742	TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1187	0.18156815536237914	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	1017	0.15556429149413611	TruSeq Adapter, Index 23 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	853	0.13047821105653698	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC	774	0.11839406255305936	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC	687	0.10508620280872323	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.588917153219355E-4	0.0	0.0	0.1630595228443944	0.0
2	4.588917153219355E-4	0.0	0.0	0.6329646393340563	0.0
3	4.588917153219355E-4	0.0	0.0	1.141416659910761	0.0
4	4.588917153219355E-4	0.0	0.0	1.748224471471467	0.0
5	4.588917153219355E-4	0.0	0.0	3.2555307923989174	0.0
6	4.588917153219355E-4	0.0	0.0	4.999778202337595	0.0
7	4.588917153219355E-4	0.0	0.0	6.250411090494976	0.0
8	4.588917153219355E-4	0.0	0.0	7.8401649562752675	0.0
9	4.588917153219355E-4	0.0	0.0	8.7550420727221	0.0
10	4.588917153219355E-4	0.0	0.0	10.05814158033129	0.0
11	4.588917153219355E-4	0.0	0.0	11.278181687467209	0.0
12	4.588917153219355E-4	0.0	0.0	12.346481600736674	0.0
13	4.588917153219355E-4	0.0	0.0	12.928968151385318	0.0
14	4.588917153219355E-4	0.0	0.0	13.299752657365442	0.0
15	4.588917153219355E-4	0.0	0.0	13.633213970499382	0.0
16	4.588917153219355E-4	0.0	0.0	14.152067536623383	0.0
17	4.588917153219355E-4	0.0	0.0	14.719716588476617	0.0
18	4.588917153219355E-4	0.0	0.0	15.377614344343165	0.0
19	4.588917153219355E-4	0.0	0.0	15.809431448461106	0.0
20	4.588917153219355E-4	0.0	0.0	16.22579919816321	0.0
21	4.588917153219355E-4	0.0	0.0	16.718190008703647	0.0
22	4.588917153219355E-4	0.0	0.0	17.250198470666877	0.0
23	6.118556204292473E-4	0.0	0.0	17.78098322138925	0.0
24	6.118556204292473E-4	0.0	0.0	18.234674163937537	0.0
25	6.118556204292473E-4	0.0	0.0	18.629168075209293	0.0
26	6.118556204292473E-4	0.0	0.0	19.020449744473797	0.0
27	6.118556204292473E-4	0.0	0.0	19.43574674684015	0.0
28	6.118556204292473E-4	0.0	0.0	19.857621197126115	0.0
29	6.118556204292473E-4	0.0	0.0	20.28744977047766	0.0
30	6.118556204292473E-4	0.0	0.0	20.826188644265613	0.0
31	6.118556204292473E-4	0.0	0.0	21.27880883947815	0.0
32	6.118556204292473E-4	0.0	0.0	21.746572461296306	0.0
33	6.118556204292473E-4	0.0	0.0	22.206534923953996	0.0
34	6.118556204292473E-4	0.0	0.0	22.653954346392883	0.0
35	6.118556204292473E-4	0.0	0.0	23.134261008429842	0.0
36	6.118556204292473E-4	0.0	0.0	23.574338163423576	0.0
37	6.118556204292473E-4	0.0	0.0	24.016709776993924	0.0
38	6.118556204292473E-4	0.0	0.0	24.538928549030285	0.0
39	6.118556204292473E-4	0.0	0.0	25.161644606722152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	3.8889524E-5	45.000004	1
TCGCAAG	30	2.164059E-6	45.000004	1
GCGTAAG	50	2.1827873E-11	45.000004	1
GTACGAG	30	2.164059E-6	45.000004	1
CGACGGT	45	3.8380676E-10	45.0	28
ATATGCG	20	7.031052E-4	45.0	1
CGGTCTA	45	3.8380676E-10	45.0	31
CGTTTTT	5830	0.0	44.150944	1
ATACGGA	50	1.0804797E-9	40.500004	4
CGTTTCT	285	0.0	39.473686	1
CGACAGG	80	0.0	39.375	2
GCGATAT	40	3.4551704E-7	39.375	9
TAGCTAG	80	0.0	39.375	1
TTACGGG	115	0.0	39.130436	3
CCGATAC	35	6.244156E-6	38.57143	11
TGTTGCG	35	6.244156E-6	38.57143	1
ATGCGCG	35	6.244156E-6	38.57143	1
CGCCCAA	70	0.0	38.57143	37
CGTGCGG	105	0.0	38.571426	2
GTTTTTT	6775	0.0	38.19188	2
>>END_MODULE