FastQCFastQC Report
Sat 14 Jan 2017
SRR2935485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231980
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC17830.7686007414432279TruSeq Adapter, Index 27 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC16880.7276489352530391TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC15490.6677299767221312No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG14320.6172945943615828No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC10180.43883093370118115TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT7230.31166479868954217TruSeq Adapter, Index 27 (100% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.29269764634882317No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC4220.18191223381325977TruSeq Adapter, Index 27 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT3570.15389257694628847No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC3330.14354685748771445TruSeq Adapter, Index 27 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG3100.13363220967324768TruSeq Adapter, Index 27 (100% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT2900.12501077679110267No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT2830.12199327528235192No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC2780.11983791706181568No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2770.11940684541770841No Hit
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT2730.11768255884127943TruSeq Adapter, Index 27 (100% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA2690.1159582722648504No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC2630.11337184240020691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTAG302.1579872E-645.0000041
GCGAACC302.1579872E-645.00000433
GCGCGAC302.1579872E-645.0000049
CGCGACC302.1579872E-645.00000410
AGGGCGT302.1579872E-645.0000046
CGGGTCC253.8811508E-545.06
AGGTACG253.8811508E-545.01
GGTAACT207.0216344E-445.08
GTTTACG207.0216344E-445.01
GGGTACT207.0216344E-445.07
GTTAGCG207.0216344E-445.01
TACGGGA551.8189894E-1245.04
AACGGGA351.2063356E-745.04
GGGCGTA207.0216344E-445.07
TACGAGG207.0216344E-445.02
TAGGGCG453.8198777E-1045.05
GCGGCTA207.0216344E-445.024
CGCAAGG351.2063356E-745.02
CGCCGCA207.0216344E-445.018
TCAAGTA207.0216344E-445.024
Produced by FastQC (version 0.11.5)