##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935485.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231980
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07617467023019	31.0	30.0	33.0	28.0	34.0
2	31.06845417708423	31.0	31.0	34.0	28.0	34.0
3	30.89497801534615	31.0	30.0	34.0	27.0	34.0
4	34.75430209500819	35.0	35.0	37.0	32.0	37.0
5	34.75077161824295	35.0	35.0	37.0	32.0	37.0
6	34.92762738167083	36.0	35.0	37.0	32.0	37.0
7	35.36369945684973	37.0	35.0	37.0	33.0	37.0
8	34.99477110095698	37.0	35.0	37.0	32.0	37.0
9	36.76408311061299	39.0	37.0	39.0	32.0	39.0
10	36.189943098542976	37.0	35.0	39.0	32.0	39.0
11	36.55727217863609	38.0	35.0	39.0	32.0	39.0
12	36.6873954651263	39.0	35.0	39.0	33.0	39.0
13	36.656332442451934	39.0	35.0	39.0	33.0	39.0
14	37.622971807914475	39.0	36.0	41.0	33.0	41.0
15	37.82513147685145	39.0	37.0	41.0	33.0	41.0
16	37.7999051642383	39.0	36.0	41.0	33.0	41.0
17	37.73849900853522	39.0	36.0	41.0	33.0	41.0
18	37.760026726441936	39.0	36.0	41.0	33.0	41.0
19	37.62597206655746	39.0	36.0	40.0	33.0	41.0
20	37.705000431071646	39.0	36.0	41.0	33.0	41.0
21	37.69072333821881	39.0	36.0	41.0	33.0	41.0
22	37.82664453832227	39.0	36.0	41.0	33.0	41.0
23	37.883636520389686	40.0	36.0	41.0	34.0	41.0
24	37.85746616087594	40.0	36.0	41.0	33.0	41.0
25	37.46969135270282	39.0	36.0	41.0	33.0	41.0
26	37.5631821708768	39.0	36.0	41.0	33.0	41.0
27	37.55739287869644	39.0	36.0	41.0	33.0	41.0
28	37.47879127510992	39.0	36.0	41.0	33.0	41.0
29	37.44250797482542	39.0	36.0	41.0	33.0	41.0
30	37.20131045779809	39.0	35.0	41.0	32.0	41.0
31	37.23642124321062	39.0	35.0	41.0	32.0	41.0
32	37.240025002155356	39.0	35.0	41.0	32.0	41.0
33	37.23707647210966	39.0	35.0	41.0	32.0	41.0
34	37.21061298387792	40.0	35.0	41.0	32.0	41.0
35	37.2462324338305	40.0	35.0	41.0	32.0	41.0
36	37.10699198206742	40.0	35.0	41.0	32.0	41.0
37	37.09492628674886	40.0	35.0	41.0	32.0	41.0
38	36.927347185102164	39.0	35.0	41.0	31.0	41.0
39	36.85337098025692	39.0	35.0	41.0	31.0	41.0
40	36.7234890938874	39.0	35.0	41.0	31.0	41.0
41	36.58129149064575	39.0	35.0	41.0	30.0	41.0
42	36.66949737046297	39.0	35.0	41.0	31.0	41.0
43	36.61871713078714	39.0	35.0	41.0	31.0	41.0
44	36.60990602638159	39.0	35.0	41.0	31.0	41.0
45	36.583671006121214	39.0	35.0	41.0	31.0	41.0
46	36.45520734546081	39.0	35.0	40.0	31.0	41.0
47	36.41769118027416	39.0	35.0	40.0	31.0	41.0
48	36.416497111819986	39.0	35.0	40.0	31.0	41.0
49	36.39750409518062	39.0	35.0	40.0	31.0	41.0
50	36.26129838779205	39.0	35.0	40.0	30.0	41.0
51	35.06389774980602	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	17.0
11	7.0
12	7.0
13	8.0
14	12.0
15	6.0
16	19.0
17	33.0
18	71.0
19	105.0
20	252.0
21	458.0
22	688.0
23	905.0
24	1213.0
25	1413.0
26	1773.0
27	2101.0
28	2449.0
29	2975.0
30	3858.0
31	5039.0
32	6646.0
33	9467.0
34	16677.0
35	18378.0
36	19362.0
37	29100.0
38	51805.0
39	57118.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24010690576774	20.30735408224847	22.932580394861628	11.519958617122166
2	32.965341839813775	25.663850331925165	23.796447969652558	17.574359858608503
3	31.955340977670488	25.01379429261143	26.579015432364862	16.45184929735322
4	29.317613587378222	24.415897922234674	27.38943012328649	18.877058367100613
5	26.56004828002414	28.776187602379515	24.650831968273128	20.012932149323216
6	24.054228812828693	35.70092249331839	24.886197085955686	15.358651607897233
7	74.49564617639452	6.5992757996378995	13.18906802310544	5.716010000862144
8	72.51788947323044	8.556772135528925	11.38115354771963	7.544184843520993
9	65.92982153633933	9.076644538322268	14.190016380722476	10.803517544615914
10	37.222174325372876	24.578843003707217	22.327786878179154	15.871195792740753
11	30.195275454780585	24.953444262436417	25.196137598068795	19.6551426847142
12	27.03983101991551	20.37158375722045	31.547116130700925	21.04146909216312
13	24.240020691438918	24.39779291318217	32.03638244676265	19.32580394861626
14	19.215449607724803	29.89136994568497	28.68307612725235	22.210104319337873
15	17.510992326924736	25.659108543839988	36.91180274161566	19.91809638761962
16	19.425812570049143	24.629278386067767	32.24200362100181	23.702905422881283
17	19.218898180877662	25.273730494008106	29.569359427536856	25.93801189757738
18	20.82334684024485	25.41425984998707	31.03974480558669	22.722648504181393
19	21.63979653418398	27.035520303474435	27.827398913699458	23.497284248642124
20	23.36882489869816	26.078110181912233	30.02543322700233	20.527631692387274
21	21.940253470126734	28.916716958358478	28.914130528493835	20.228899043020952
22	19.132252780412102	25.994913354599536	28.503319251659626	26.369514613328736
23	19.622812311406157	27.43253728769722	29.255108198982672	23.68954220191396
24	21.401844986636778	24.66031554444349	29.43055435813432	24.50728511078541
25	18.63307181653591	28.436503146823	27.30623329597379	25.6241917406673
26	18.017501508750755	26.34494352961462	29.280541425984996	26.357013535649624
27	21.23545133201138	27.685145271144062	27.440296577291146	23.639106819553408
28	17.078196396241054	27.71833778774032	29.043020950081903	26.160444865936718
29	19.143029571514784	24.86507457539443	28.198120527631694	27.79377532545909
30	19.812914906457454	28.210621605310806	28.249418053280458	23.72704543495129
31	19.844814208121388	25.62548495559962	26.430726786791965	28.098974049487026
32	20.952237261832916	26.146650573325285	26.126390206052246	26.77472195878955
33	19.936632468316233	24.9344771100957	28.118372273471852	27.01051814811622
34	17.976980774204673	22.493749461160444	31.4863350288818	28.042934735753082
35	17.684714199499957	23.987412707992068	29.82282955427192	28.505043538236052
36	19.039572376929044	25.452194154668504	28.474437451504443	27.03379601689801
37	18.2701094921976	25.991464781446677	30.669885334942666	25.068540391413052
38	17.943357185964306	24.86722993361497	28.6860936287611	28.503319251659626
39	20.549616346236746	21.81524269333563	30.434520217260108	27.200620743167512
40	20.374170187085095	21.464350375032332	34.130959565479785	24.030519872402795
41	16.747995516854903	24.19562031209587	30.47288559358565	28.583498577463573
42	18.875765152168288	23.006724717648073	30.53797741184585	27.57953271833779
43	20.396585912578672	23.58134321924304	28.085179756875593	27.936891111302696
44	19.593499439606862	22.91016466936805	28.8943012328649	28.602034658160186
45	18.844727993792567	22.529097335977237	28.336494525390123	30.28968014484007
46	21.194930597465298	24.373652901112163	29.444348650745756	24.987067850676784
47	17.316579015432364	23.056729028364515	33.89559444779722	25.7310975084059
48	18.42270885421157	22.04931459608587	30.701784636606604	28.826191913095954
49	18.757220450038798	20.817742908871452	33.6563496853177	26.768686955772047
50	18.663677903267523	20.69919820674196	32.30019829295629	28.33692559703423
51	17.521769118027418	21.02551944133115	29.534011552720063	31.91869988792137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	250.5
2	355.0
3	758.0
4	1161.0
5	791.0
6	421.0
7	397.5
8	374.0
9	383.5
10	393.0
11	397.5
12	402.0
13	451.5
14	501.0
15	454.0
16	407.0
17	443.5
18	480.0
19	485.5
20	491.0
21	535.5
22	580.0
23	666.5
24	753.0
25	1077.5
26	1534.0
27	1666.0
28	2134.5
29	2603.0
30	3060.5
31	3518.0
32	4046.5
33	4575.0
34	5246.0
35	5917.0
36	6011.0
37	6105.0
38	6788.5
39	7472.0
40	8686.0
41	9900.0
42	11466.0
43	13032.0
44	14552.0
45	16072.0
46	21682.0
47	27292.0
48	27093.5
49	26895.0
50	26412.5
51	25930.0
52	21633.0
53	17336.0
54	14802.5
55	12269.0
56	11030.5
57	9792.0
58	9174.5
59	8557.0
60	8471.0
61	8385.0
62	7391.0
63	6397.0
64	5180.5
65	3964.0
66	3097.0
67	2230.0
68	2014.0
69	1798.0
70	1395.5
71	993.0
72	813.5
73	634.0
74	529.5
75	314.5
76	204.0
77	137.0
78	70.0
79	48.5
80	27.0
81	34.0
82	41.0
83	25.5
84	10.0
85	6.0
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	231980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.79144753858091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63541446506319	30.444865936718685
2	10.385932282199771	8.473144236572118
3	3.690239675360359	4.515906543667557
4	1.91486663566809	3.1244072764893525
5	1.2607262121148075	2.57134235709975
6	0.9827957898296488	2.4053797741184586
7	0.7883501711966859	2.251056125528063
8	0.7038085978780065	2.2967497198034312
9	0.619267024559327	2.2734718510216396
>10	4.913978949148244	33.49469781877748
>50	0.06763325865494357	1.9527545478058455
>100	0.029589550661537813	2.371756185878093
>500	0.0021135393329669867	0.6043624450383654
>1k	0.005283848332417466	3.2201051814811623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1783	0.7686007414432279	TruSeq Adapter, Index 27 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1688	0.7276489352530391	TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	1549	0.6677299767221312	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	1432	0.6172945943615828	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1018	0.43883093370118115	TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	723	0.31166479868954217	TruSeq Adapter, Index 27 (100% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.29269764634882317	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	422	0.18191223381325977	TruSeq Adapter, Index 27 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	357	0.15389257694628847	No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	333	0.14354685748771445	TruSeq Adapter, Index 27 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	310	0.13363220967324768	TruSeq Adapter, Index 27 (100% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	290	0.12501077679110267	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	283	0.12199327528235192	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	278	0.11983791706181568	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	277	0.11940684541770841	No Hit
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	273	0.11768255884127943	TruSeq Adapter, Index 27 (100% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA	269	0.1159582722648504	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	263	0.11337184240020691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.310716441072506E-4	0.0	0.0	0.32804552116561775	0.0
2	4.310716441072506E-4	0.0	0.0	1.9510302612294164	0.0
3	4.310716441072506E-4	0.0	0.0	2.5653073540822486	0.0
4	4.310716441072506E-4	0.0	0.0	3.459349943960686	0.0
5	4.310716441072506E-4	0.0	0.0	6.1117337701525996	0.0
6	4.310716441072506E-4	0.0	0.0	7.336408311061298	0.0
7	4.310716441072506E-4	0.0	0.0	8.629192171738943	0.0
8	4.310716441072506E-4	0.0	0.0	10.563410638848177	0.0
9	4.310716441072506E-4	0.0	0.0	11.235020260367273	0.0
10	4.310716441072506E-4	0.0	0.0	13.315372014828865	0.0
11	8.621432882145012E-4	0.0	0.0	15.23665833261488	0.0
12	8.621432882145012E-4	0.0	0.0	17.45840158634365	0.0
13	8.621432882145012E-4	0.0	0.0	18.208897318734373	0.0
14	8.621432882145012E-4	0.0	0.0	18.55590999224071	0.0
15	8.621432882145012E-4	0.0	0.0	19.229243900336236	0.0
16	8.621432882145012E-4	0.0	0.0	20.31985515992758	0.0
17	8.621432882145012E-4	0.0	0.0	21.604448659367186	0.0
18	8.621432882145012E-4	0.0	0.0	22.995085783257178	0.0
19	8.621432882145012E-4	0.0	0.0	23.85550478489525	0.0
20	8.621432882145012E-4	0.0	0.0	24.598241227692043	0.0
21	8.621432882145012E-4	0.0	0.0	25.461677730838865	0.0
22	8.621432882145012E-4	0.0	0.0	26.3794292611432	0.0
23	8.621432882145012E-4	0.0	0.0	27.27217863608932	0.0
24	8.621432882145012E-4	0.0	0.0	28.00586257435986	0.0
25	8.621432882145012E-4	0.0	0.0	28.636520389688766	0.0
26	8.621432882145012E-4	0.0	0.0	29.24734890938874	0.0
27	8.621432882145012E-4	0.0	0.0	29.857746357444608	0.0
28	8.621432882145012E-4	0.0	0.0	30.456935942753685	0.0
29	8.621432882145012E-4	0.0	0.0	31.011725148719716	0.0
30	8.621432882145012E-4	0.0	0.0	31.653590826795412	0.0
31	8.621432882145012E-4	0.0	0.0	32.25709112854556	0.0
32	8.621432882145012E-4	0.0	0.0	32.86921286317786	0.0
33	8.621432882145012E-4	0.0	0.0	33.45719458574015	0.0
34	8.621432882145012E-4	0.0	0.0	34.033106302267434	0.0
35	8.621432882145012E-4	0.0	0.0	34.627554099491334	0.0
36	0.001293214932321752	0.0	0.0	35.218553323562375	0.0
37	0.001293214932321752	0.0	0.0	35.76170359513751	0.0
38	0.001293214932321752	0.0	0.0	36.302698508492114	0.0
39	0.001293214932321752	0.0	0.0	36.95663419260281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTAG	30	2.1579872E-6	45.000004	1
GCGAACC	30	2.1579872E-6	45.000004	33
GCGCGAC	30	2.1579872E-6	45.000004	9
CGCGACC	30	2.1579872E-6	45.000004	10
AGGGCGT	30	2.1579872E-6	45.000004	6
CGGGTCC	25	3.8811508E-5	45.0	6
AGGTACG	25	3.8811508E-5	45.0	1
GGTAACT	20	7.0216344E-4	45.0	8
GTTTACG	20	7.0216344E-4	45.0	1
GGGTACT	20	7.0216344E-4	45.0	7
GTTAGCG	20	7.0216344E-4	45.0	1
TACGGGA	55	1.8189894E-12	45.0	4
AACGGGA	35	1.2063356E-7	45.0	4
GGGCGTA	20	7.0216344E-4	45.0	7
TACGAGG	20	7.0216344E-4	45.0	2
TAGGGCG	45	3.8198777E-10	45.0	5
GCGGCTA	20	7.0216344E-4	45.0	24
CGCAAGG	35	1.2063356E-7	45.0	2
CGCCGCA	20	7.0216344E-4	45.0	18
TCAAGTA	20	7.0216344E-4	45.0	24
>>END_MODULE