##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935483.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346839
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219398625875407	31.0	31.0	33.0	28.0	34.0
2	31.21495275906112	31.0	31.0	34.0	28.0	34.0
3	31.02857521789649	31.0	31.0	34.0	27.0	34.0
4	34.85690478867717	35.0	35.0	37.0	32.0	37.0
5	34.90554983724437	35.0	35.0	37.0	33.0	37.0
6	35.0861235328207	37.0	35.0	37.0	32.0	37.0
7	35.48820634357728	37.0	35.0	37.0	33.0	37.0
8	35.25518756541219	37.0	35.0	37.0	32.0	37.0
9	37.03829730797286	39.0	37.0	39.0	33.0	39.0
10	36.47813250528343	38.0	35.0	39.0	32.0	39.0
11	36.75685548626308	39.0	37.0	39.0	33.0	39.0
12	36.80817324464665	39.0	35.0	39.0	33.0	39.0
13	36.82564244505376	39.0	36.0	39.0	33.0	39.0
14	37.721816174075	40.0	37.0	41.0	33.0	41.0
15	37.945205123991244	40.0	37.0	41.0	33.0	41.0
16	37.89413531926917	39.0	37.0	41.0	33.0	41.0
17	37.87822592038381	39.0	37.0	41.0	33.0	41.0
18	37.863259322048556	39.0	37.0	41.0	33.0	41.0
19	37.74104411556947	39.0	36.0	41.0	33.0	41.0
20	37.87929269776467	39.0	36.0	41.0	33.0	41.0
21	37.78972952868622	39.0	36.0	41.0	33.0	41.0
22	37.93491216385701	40.0	36.0	41.0	34.0	41.0
23	38.02986976666407	40.0	36.0	41.0	34.0	41.0
24	38.02309140552245	40.0	36.0	41.0	34.0	41.0
25	37.60787281707074	39.0	36.0	41.0	33.0	41.0
26	37.7143516155911	39.0	36.0	41.0	33.0	41.0
27	37.74087689100707	40.0	36.0	41.0	33.0	41.0
28	37.61141624788446	40.0	36.0	41.0	33.0	41.0
29	37.54127131031977	40.0	36.0	41.0	33.0	41.0
30	37.29260261965926	39.0	35.0	41.0	32.0	41.0
31	37.22630096384778	39.0	35.0	41.0	32.0	41.0
32	37.26539114690101	39.0	35.0	41.0	33.0	41.0
33	37.28041252569636	39.0	35.0	41.0	33.0	41.0
34	37.106400952603366	39.0	35.0	41.0	32.0	41.0
35	37.13579211103711	39.0	35.0	41.0	32.0	41.0
36	36.965975567914796	39.0	35.0	41.0	31.0	41.0
37	36.924342418240165	39.0	35.0	41.0	31.0	41.0
38	36.7572216503911	39.0	35.0	41.0	31.0	41.0
39	36.697156317484485	39.0	35.0	41.0	31.0	41.0
40	36.578648883199406	39.0	35.0	41.0	31.0	41.0
41	36.39976473233979	39.0	35.0	40.0	30.0	41.0
42	36.45509588022108	39.0	35.0	40.0	30.0	41.0
43	36.416556384950944	39.0	35.0	40.0	31.0	41.0
44	36.38781105930994	39.0	35.0	40.0	31.0	41.0
45	36.371547029024995	39.0	35.0	40.0	31.0	41.0
46	36.274398207814	38.0	35.0	40.0	30.0	41.0
47	36.271013352016354	38.0	35.0	40.0	31.0	41.0
48	36.19490311066518	38.0	35.0	40.0	31.0	41.0
49	36.13796603034837	38.0	35.0	40.0	30.0	41.0
50	36.00202687702363	38.0	35.0	40.0	30.0	41.0
51	34.75172630528862	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	15.0
10	17.0
11	10.0
12	12.0
13	17.0
14	15.0
15	13.0
16	29.0
17	53.0
18	104.0
19	184.0
20	315.0
21	550.0
22	772.0
23	1102.0
24	1570.0
25	2069.0
26	2708.0
27	3315.0
28	3842.0
29	4525.0
30	5641.0
31	7399.0
32	9900.0
33	14477.0
34	24887.0
35	26416.0
36	29727.0
37	45978.0
38	78390.0
39	82760.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.79997347472458	21.033101813809864	23.00894651408867	13.157978197376883
2	33.434533025409486	23.227491718059387	24.571342899731576	18.766632356799555
3	33.586476722629236	23.04671619973532	26.740072483198258	16.62673459443719
4	29.260261965926553	25.221212147422868	25.3042477922033	20.21427809444728
5	24.967780439915927	30.02488186161302	24.945868255876647	20.0614694425944
6	24.63938599753776	33.73813210163794	25.482428446627974	16.140053454196327
7	76.87457292864988	6.170009716323712	10.80328336778736	6.152133987239036
8	76.90859447755298	5.398758501783249	11.082663714288184	6.609983306375581
9	69.96272045531212	7.79352956270777	14.498081242305508	7.745668739674604
10	39.16255092420403	24.27754664267859	20.872796888469868	15.687105544647517
11	29.66361914317594	25.088585770343013	24.903197160642257	20.34459792583879
12	26.759966439760234	20.814556609839148	31.068017149167193	21.357459801233425
13	25.83907807368837	23.14186120937957	31.48406032770248	19.535000389229584
14	20.41119943258976	25.88492066924423	31.95690219381327	21.74697770435274
15	19.266287816537357	24.56528821729967	35.787497945732746	20.38092602043023
16	22.801934038559676	25.13702322979826	30.454764314278382	21.606278417363676
17	23.203849624753847	24.026133162648954	27.867973324799117	24.902043887798083
18	24.81497178806305	23.504565518871868	30.792673257620972	20.88778943544411
19	24.46437684343456	25.85407062066261	27.556013020450408	22.125539515452413
20	24.40959638333636	25.97112781434614	28.37426010339091	21.245015698926593
21	23.766358454499063	26.710087389249765	29.93953967114425	19.584014485106923
22	22.58973183523191	24.02959298118147	29.468139396088677	23.912535787497944
23	20.93968671343188	26.436761725180848	29.261415238770727	23.362136322616543
24	21.874990990055903	25.293291700183655	29.183281003578028	23.648436306182408
25	21.497582451800405	26.687598568788403	26.73949584677617	25.075323132635024
26	20.675587232116342	25.879154305023366	28.12198166872814	25.32327679413215
27	22.311504761575254	24.959419211795677	29.311294289281193	23.417781737347877
28	19.65378749217937	25.53922713420348	29.95885699128414	24.848128382333012
29	21.159673508457814	26.632529790479154	29.334071427953607	22.873725273109425
30	25.599773958522544	23.92118533382924	28.7669495068317	21.712091200816516
31	24.45630393352535	25.40804234817884	28.447204610784834	21.688449107510976
32	25.027173991390818	26.36929526379675	28.059416616931777	20.544114127880658
33	26.796871170773763	23.161755165941546	27.545345246641812	22.49602841664288
34	21.853943760649756	23.838438007259853	32.614844351413765	21.692773880676626
35	24.08293185022446	24.801997468566107	29.33839620111925	21.776674480090186
36	27.857305550990517	25.51817990479733	27.05606924250156	19.56844530171059
37	23.345990502798127	25.73326529023553	29.980192538901335	20.940551668065012
38	24.308685009471255	25.61130668696427	28.161192945430013	21.918815358134466
39	25.326736612664664	22.071047373565257	29.520324992287488	23.08189102148259
40	26.88048345197628	21.44712676486785	30.762976481883523	20.909413301272348
41	23.698891993114962	23.254593629897446	27.774269906210087	25.272244470777505
42	24.762209555442148	22.795302719705685	29.240368009364577	23.20211971548759
43	24.864562520362472	22.342643128367918	29.93665648903382	22.85613786223579
44	23.464200969325827	22.37839458653727	29.659582688221338	24.49782175591557
45	23.883127329971543	22.11688996912112	28.688238635216916	25.311744065690423
46	24.36404210599154	23.23037490016982	29.067377082738677	23.338205911099962
47	22.054613235535797	23.50110570033935	31.41832377558464	23.025957288540216
48	22.926775823941366	23.301012861875396	30.126081553689176	23.646129760494063
49	25.49482612970283	21.910742448225257	30.49714709130173	22.097284330770183
50	22.618563656336224	22.228757435005868	30.875132265979317	24.27754664267859
51	22.599246336196334	21.4514515380335	28.425292426745553	27.52400969902462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	358.0
2	555.0
3	1398.0
4	2241.0
5	1444.0
6	647.0
7	631.0
8	615.0
9	652.0
10	689.0
11	721.5
12	754.0
13	715.0
14	676.0
15	664.0
16	652.0
17	674.0
18	696.0
19	657.5
20	619.0
21	777.0
22	935.0
23	1042.0
24	1149.0
25	1408.0
26	2033.5
27	2400.0
28	2947.5
29	3495.0
30	4217.0
31	4939.0
32	5481.0
33	6023.0
34	6934.0
35	7845.0
36	8341.0
37	8837.0
38	9743.5
39	10650.0
40	12006.5
41	13363.0
42	15180.5
43	16998.0
44	19047.5
45	21097.0
46	25767.0
47	30437.0
48	34516.0
49	38595.0
50	38999.5
51	39404.0
52	33899.5
53	28395.0
54	24931.0
55	21467.0
56	19996.5
57	18526.0
58	17107.5
59	15689.0
60	14705.0
61	13721.0
62	12111.5
63	10502.0
64	9302.0
65	8102.0
66	6803.0
67	5504.0
68	4438.5
69	3373.0
70	2911.0
71	2449.0
72	1885.0
73	1321.0
74	1068.5
75	641.0
76	466.0
77	344.0
78	222.0
79	169.5
80	117.0
81	69.0
82	21.0
83	12.0
84	3.0
85	2.0
86	1.0
87	2.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	346839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.18696299542227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71023150234403	30.35900577271053
2	10.263038024620009	8.45406734681272
3	3.7813559112005497	4.672276979614149
4	2.030379455196833	3.3450065395143045
5	1.4677324203069297	3.0225720441181543
6	1.1137380046980052	2.752289159165531
7	0.9128070603621203	2.6317025431966385
8	0.8064472962757536	2.6572091959574244
9	0.7251939556235326	2.6881682953293304
>10	5.086902590972817	33.01742323218136
>50	0.05919032679693066	1.633082543051155
>100	0.03875557111703794	2.8462129908837177
>500	0.001409293495165016	0.31373062538444974
>1k	0.002818586990330032	1.6072527320805572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1512	0.4359371350972642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1510	0.4353604986751778	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1496	0.43132404372057354	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1020	0.2940845752640274	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	555	0.1600166071289561	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	526	0.15165537900870432	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	444	0.1280132857031649	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	430	0.12397683074856058	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	408	0.11763383010561096	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	370	0.10667773808597071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.766364220863283E-4	0.0	0.0	0.3024458033842792	0.0
2	5.766364220863283E-4	0.0	0.0	1.4447625555372954	0.0
3	5.766364220863283E-4	0.0	0.0	1.960852153304559	0.0
4	5.766364220863283E-4	0.0	0.0	2.587655944112398	0.0
5	5.766364220863283E-4	0.0	0.0	4.280083842935771	0.0
6	5.766364220863283E-4	0.0	0.0	5.347726178428608	0.0
7	5.766364220863283E-4	0.0	0.0	6.3865366928171285	0.0
8	5.766364220863283E-4	0.0	0.0	8.048114543058883	0.0
9	5.766364220863283E-4	0.0	0.0	8.694235654006613	0.0
10	5.766364220863283E-4	0.0	0.0	10.033185426091068	0.0
11	5.766364220863283E-4	0.0	0.0	11.818163470659297	0.0
12	5.766364220863283E-4	0.0	0.0	13.329815851158607	0.0
13	5.766364220863283E-4	0.0	0.0	14.069640380695366	0.0
14	5.766364220863283E-4	0.0	0.0	14.390538549586408	0.0
15	5.766364220863283E-4	0.0	0.0	14.91095292051932	0.0
16	5.766364220863283E-4	0.0	0.0	15.825498285948235	0.0
17	5.766364220863283E-4	0.0	0.0	16.90582662272697	0.0
18	5.766364220863283E-4	0.0	0.0	18.080723332727864	0.0
19	5.766364220863283E-4	0.0	0.0	18.76749731143268	0.0
20	5.766364220863283E-4	0.0	0.0	19.469552155322788	0.0
21	5.766364220863283E-4	0.0	0.0	20.31115301335778	0.0
22	5.766364220863283E-4	0.0	0.0	21.1328599148308	0.0
23	5.766364220863283E-4	0.0	0.0	21.946782224605652	0.0
24	5.766364220863283E-4	0.0	0.0	22.60068792725155	0.0
25	5.766364220863283E-4	0.0	0.0	23.285731996690107	0.0
26	5.766364220863283E-4	0.0	0.0	23.845645962535933	0.0
27	5.766364220863283E-4	0.0	0.0	24.508489529724166	0.0
28	5.766364220863283E-4	0.0	0.0	25.05283431217366	0.0
29	5.766364220863283E-4	0.0	0.0	25.646481508711535	0.0
30	5.766364220863283E-4	0.0	0.0	26.40418176733297	0.0
31	5.766364220863283E-4	0.0	0.0	26.98254809868556	0.0
32	5.766364220863283E-4	0.0	0.0	27.58052006838908	0.0
33	5.766364220863283E-4	0.0	0.0	28.17820371988156	0.0
34	5.766364220863283E-4	0.0	0.0	28.822018285140945	0.0
35	5.766364220863283E-4	0.0	0.0	29.474482396731624	0.0
36	5.766364220863283E-4	0.0	0.0	30.02978327120076	0.0
37	5.766364220863283E-4	0.0	0.0	30.619682330995072	0.0
38	5.766364220863283E-4	0.0	0.0	31.266956714786975	0.0
39	5.766364220863283E-4	0.0	0.0	32.15872494154348	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	20	7.026466E-4	45.000004	2
TACCCCG	20	7.026466E-4	45.000004	29
CCGATTA	20	7.026466E-4	45.000004	11
TATGCGG	40	6.7921064E-9	45.000004	2
CAGTCTA	20	7.026466E-4	45.000004	27
CCTCTCA	20	7.026466E-4	45.000004	25
CACGACG	25	3.885154E-5	45.0	26
TTCGGCC	35	1.2086457E-7	45.0	12
AGGGCCG	25	3.885154E-5	45.0	6
CGGTCTA	30	2.161103E-6	44.999996	31
CGCCGCA	30	2.161103E-6	44.999996	18
CGTTTTT	695	0.0	42.08633	1
GTGCACG	65	0.0	41.53846	1
TGCATTA	185	0.0	41.351353	15
TGCGGGT	60	3.6379788E-12	41.249996	4
TATGGGC	105	0.0	40.714287	4
CTACGAA	50	1.0768417E-9	40.5	11
TACGAAT	50	1.0768417E-9	40.5	12
CGAATAT	50	1.0768417E-9	40.5	14
GCTACGA	50	1.0768417E-9	40.5	10
>>END_MODULE