FastQCFastQC Report
Sat 14 Jan 2017
SRR2935482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496635
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC86001.7316540316328892No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC68451.3782757961078056No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG59291.1938345062269071No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC29450.592990828274286No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27960.5629889154006463No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT22580.45465986086361215No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT19130.3851923444783392No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT13540.27263483242220143No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT11800.237599041549629No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC10910.21967843587342817No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA9610.1935022702789775No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC9080.18283044892123995No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT8000.16108409596585016No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG7230.1455797517291371No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG6420.12926998701259476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG502.1827873E-1145.01
CAATCGT207.029412E-445.041
CAATCGA406.8012014E-945.041
AGTACGG750.045.02
CCAATCG600.044.99999640
GCGGGTT600.044.9999965
CCGATGA8750.042.42857418
GATGAAT9350.041.1497320
CGTTTTT12850.041.147861
CGATGAA9100.041.04395719
ACGGGAC501.0786607E-940.55
ATACGAA501.0786607E-940.519
GATTACG451.9244908E-840.01
ATGAATG9700.039.89690821
TGCGTAG403.452933E-739.3751
TACGGCT9400.038.7765967
GTTGGCG356.2411236E-638.571431
GTAACGG356.2411236E-638.571432
ACGACGG356.2411236E-638.571432
CCACCGA11600.038.40517415
Produced by FastQC (version 0.11.5)