##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935482.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	496635
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16109416372185	31.0	31.0	33.0	28.0	34.0
2	31.167253616841343	31.0	31.0	34.0	28.0	34.0
3	30.961482779103367	31.0	30.0	34.0	27.0	34.0
4	34.825630493219364	35.0	35.0	37.0	32.0	37.0
5	34.793172047882244	35.0	35.0	37.0	32.0	37.0
6	34.972953980287336	36.0	35.0	37.0	32.0	37.0
7	35.360961269342674	37.0	35.0	37.0	33.0	37.0
8	35.03740171353207	37.0	35.0	37.0	32.0	37.0
9	36.78599172430457	39.0	37.0	39.0	32.0	39.0
10	36.24085696739054	37.0	35.0	39.0	32.0	39.0
11	36.59278745960313	38.0	35.0	39.0	32.0	39.0
12	36.665410210718136	39.0	35.0	39.0	33.0	39.0
13	36.62148861840184	39.0	35.0	39.0	32.0	39.0
14	37.58860128665922	39.0	36.0	41.0	32.0	41.0
15	37.73365348797407	40.0	37.0	41.0	33.0	41.0
16	37.84424778761062	39.0	37.0	41.0	33.0	41.0
17	37.66480815890946	39.0	36.0	41.0	33.0	41.0
18	37.671491135340844	39.0	36.0	41.0	33.0	41.0
19	37.58073836922488	39.0	36.0	40.0	33.0	41.0
20	37.458048667532495	39.0	36.0	41.0	32.0	41.0
21	37.545273691946804	39.0	36.0	41.0	33.0	41.0
22	37.67036354666908	39.0	36.0	41.0	33.0	41.0
23	37.677652602011534	40.0	36.0	41.0	33.0	41.0
24	37.70340994895648	40.0	36.0	41.0	33.0	41.0
25	37.37852144935415	39.0	35.0	41.0	32.0	41.0
26	37.39990939019602	39.0	35.0	41.0	33.0	41.0
27	37.391971971367305	39.0	35.0	41.0	33.0	41.0
28	37.2386762914414	39.0	35.0	41.0	32.0	41.0
29	37.232675908866675	39.0	35.0	41.0	32.0	41.0
30	36.99516143646743	39.0	35.0	41.0	31.0	41.0
31	37.02703997905907	39.0	35.0	41.0	31.0	41.0
32	36.930174071501206	39.0	35.0	41.0	31.0	41.0
33	36.89217634681406	39.0	35.0	41.0	31.0	41.0
34	36.707489403686814	39.0	35.0	41.0	31.0	41.0
35	36.69077290162796	39.0	35.0	41.0	31.0	41.0
36	36.59987515982563	39.0	35.0	41.0	31.0	41.0
37	36.596967591893446	39.0	35.0	41.0	31.0	41.0
38	36.45452495293324	39.0	35.0	41.0	30.0	41.0
39	36.289117762541906	39.0	35.0	41.0	30.0	41.0
40	36.22257392249841	39.0	35.0	41.0	30.0	41.0
41	35.968185891046744	39.0	35.0	40.0	28.0	41.0
42	36.04807353488981	39.0	35.0	41.0	29.0	41.0
43	36.100778237538634	39.0	35.0	41.0	30.0	41.0
44	36.20053359106789	39.0	35.0	41.0	30.0	41.0
45	36.21832935656971	39.0	35.0	40.0	30.0	41.0
46	36.104358331571476	39.0	35.0	40.0	30.0	41.0
47	35.968624845208254	38.0	35.0	40.0	30.0	41.0
48	35.91929284081871	38.0	35.0	40.0	29.0	41.0
49	35.91399720116383	38.0	35.0	40.0	29.0	41.0
50	35.781292095804766	38.0	35.0	40.0	29.0	41.0
51	34.6146103275041	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	20.0
10	30.0
11	21.0
12	23.0
13	16.0
14	18.0
15	47.0
16	44.0
17	83.0
18	169.0
19	371.0
20	818.0
21	1526.0
22	2421.0
23	3038.0
24	3792.0
25	4472.0
26	4864.0
27	5309.0
28	5519.0
29	6701.0
30	8264.0
31	10924.0
32	14776.0
33	21129.0
34	34984.0
35	36802.0
36	41081.0
37	62028.0
38	106456.0
39	120861.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.14695903430085	19.419090478923152	20.45788154278293	10.976068943993074
2	33.10056681466268	29.091586376312584	21.641446937892013	16.16639987113272
3	30.058896372587512	29.394827186968296	24.983337863823532	15.562938576620658
4	26.882116645020993	22.408811300049333	32.13768663102681	18.571385423902868
5	28.64518207536722	27.245763991663896	24.027907819626083	20.081146113342797
6	22.919447884261075	37.16592668660083	25.533037341306997	14.381588087831105
7	73.17124246176769	5.635124387125353	16.425342555397826	4.768290595709122
8	72.45180061816022	10.342605736607368	11.384215772146545	5.821377873085868
9	66.07850836127136	6.972726449001781	13.374007067564712	13.57475812216215
10	40.30485165161537	21.219003896221572	22.169601417540044	16.306543034623015
11	31.87854259164175	23.860581714941556	24.892526704722783	19.36834898869391
12	25.37759118869995	19.43056772076072	31.379383249267573	23.81245784127176
13	26.57343924612643	21.121346663042274	34.505421486604845	17.799792604226443
14	21.139065913598518	28.004067373423137	30.53953104392562	20.317335669052724
15	18.83999315392592	23.488880163500358	36.45252549659207	21.218601185981655
16	22.13476698178743	24.683922800447007	28.47221802732389	24.709092190441673
17	22.092885116836307	26.072467707672637	28.805259395733284	23.02938777975777
18	22.797426681566947	23.558549035005587	30.091113191780682	23.552911091646784
19	21.758434262587212	26.099650648866874	27.684315442930924	24.457599645614987
20	23.140535805974206	26.701903812659193	30.506307449132663	19.651252932233934
21	22.372567378457013	28.43335648917213	30.676452525496593	18.517623606874263
22	20.381970662559024	22.612179971206217	32.640671720680174	24.36517764555458
23	21.240548894057003	26.218047459401774	30.517381980730313	22.02402166581091
24	22.061071007883054	25.861648897077334	28.299052624160602	23.778227470879017
25	20.778841603994884	29.812437705759763	27.1617989066417	22.24692178360365
26	19.338951141180143	24.026498333786385	30.940227732640675	25.694322792392803
27	22.996768250324685	24.708890835321714	29.107292075669257	23.187048838684348
28	18.668035881482375	25.533037341306997	31.936532866189456	23.862393911021172
29	20.606682976431383	23.46471754910548	32.71295820874485	23.215641265718283
30	20.986237377550918	25.053409445568676	32.75745769025542	21.202895486624985
31	22.580768572492875	23.052342263432905	30.353277557965104	24.013611606109116
32	23.80279279551381	25.593846587534102	27.634782083421428	22.96857853353066
33	22.648423892798533	22.58298347881241	27.52443947768482	27.244153150704243
34	23.047711095673883	22.829844855880072	30.35911685644387	23.763327192002173
35	20.936704018041418	26.38758847040583	29.094405347991987	23.581302163560764
36	21.061544192414953	27.969434292790478	30.23910920494931	20.729912309845258
37	20.02617616559445	25.16053036938597	31.751084800708767	23.062208664310813
38	19.567489202331693	25.777281101815213	27.759823612914918	26.895406082938173
39	22.778096590051046	24.03998912682352	26.988834858598366	26.193079424527067
40	22.66312281655542	23.595195666837817	28.855598175722612	24.88608334088415
41	20.75226272816052	26.678546618744146	27.46161668025814	25.107573972837194
42	19.85482295851078	25.96373594289569	31.398511985663514	22.78292911293002
43	21.093559656488164	24.999043563180205	29.353952097616965	24.55344468271467
44	21.384920515066398	22.579560441773133	30.289045274698722	25.746473768461748
45	20.480433316218146	21.652521469489667	28.543095029548866	29.323950184743325
46	23.983811048355435	24.435249227299728	27.766065621633594	23.814874102711247
47	19.23042073152315	24.121336595286277	33.546971115608045	23.101271557582532
48	20.141149939090074	25.687476718314254	29.013460589769146	25.157912752826523
49	21.154972968075146	22.194166742174836	33.08969363818498	23.56116665156503
50	20.65037703746212	22.46136498635819	31.495967863722857	25.392290112456834
51	20.59037321171484	22.245310942643997	28.58034572674097	28.583970118900197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	357.0
2	559.0
3	2353.0
4	4147.0
5	2653.5
6	1160.0
7	1100.5
8	1041.0
9	1041.0
10	1041.0
11	1047.0
12	1053.0
13	1057.0
14	1061.0
15	1039.0
16	1017.0
17	1069.0
18	1121.0
19	1080.0
20	1039.0
21	1191.0
22	1343.0
23	1544.0
24	1745.0
25	2094.0
26	2719.5
27	2996.0
28	3595.5
29	4195.0
30	5132.5
31	6070.0
32	7599.0
33	9128.0
34	10247.0
35	11366.0
36	12146.0
37	12926.0
38	14907.5
39	16889.0
40	18648.5
41	20408.0
42	24273.5
43	28139.0
44	46954.5
45	65770.0
46	57606.0
47	49442.0
48	50385.0
49	51328.0
50	44947.5
51	38567.0
52	35523.0
53	32479.0
54	30348.5
55	28218.0
56	25688.0
57	23158.0
58	21962.5
59	20767.0
60	18363.0
61	15959.0
62	14627.5
63	13296.0
64	11241.5
65	9187.0
66	7692.0
67	6197.0
68	5340.5
69	4484.0
70	3613.5
71	2743.0
72	2283.0
73	1823.0
74	1436.5
75	837.5
76	625.0
77	435.0
78	245.0
79	189.5
80	134.0
81	96.5
82	59.0
83	53.5
84	48.0
85	28.5
86	9.0
87	5.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	496635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.69927419602407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65073972011221	27.76579431448511
2	9.84895144506234	7.425966421414653
3	3.674876026180449	4.156204768421162
4	2.13002046783879	3.212009026407921
5	1.4721699002580906	2.774986836648158
6	1.1374362714509099	2.572831312675867
7	0.9644323114166558	2.5450878706121243
8	0.8418589066534942	2.5389975805036085
9	0.7113862573231619	2.4136871016698294
>10	5.473137135381677	33.22602939460267
>50	0.05775054966182068	1.5016257096485865
>100	0.029145137212506913	1.9425502235792398
>500	0.0026986238159728624	0.8208073323471932
>1k	0.0037780733423620075	2.754404773917688
>5k	0.0016191742895837174	4.349017333066164
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	8600	1.7316540316328892	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	6845	1.3782757961078056	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	5929	1.1938345062269071	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2945	0.592990828274286	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2796	0.5629889154006463	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	2258	0.45465986086361215	No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	1913	0.3851923444783392	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	1354	0.27263483242220143	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT	1180	0.237599041549629	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC	1091	0.21967843587342817	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA	961	0.1935022702789775	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	908	0.18283044892123995	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	800	0.16108409596585016	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	723	0.1455797517291371	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	642	0.12926998701259476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.013551199573127E-4	0.0	0.0	0.1677288149244415	0.0
2	2.013551199573127E-4	0.0	0.0	1.1034260573660737	0.0
3	2.013551199573127E-4	0.0	0.0	1.4723086371278706	0.0
4	2.013551199573127E-4	0.0	0.0	2.2739033696779325	0.0
5	2.013551199573127E-4	0.0	0.0	5.151469388987889	0.0
6	2.013551199573127E-4	0.0	0.0	6.434302858235927	0.0
7	2.013551199573127E-4	0.0	0.0	7.395974911152053	0.0
8	2.013551199573127E-4	0.0	0.0	8.674378567761032	0.0
9	2.013551199573127E-4	0.0	0.0	9.083129461274376	0.0
10	2.013551199573127E-4	0.0	0.0	11.226353358100013	0.0
11	2.013551199573127E-4	0.0	0.0	12.53334944174293	0.0
12	2.013551199573127E-4	0.0	0.0	14.998137465140395	0.0
13	2.013551199573127E-4	0.0	0.0	15.599383853332931	0.0
14	2.013551199573127E-4	0.0	0.0	15.885509478792272	0.0
15	2.013551199573127E-4	0.0	0.0	16.437021152355353	0.0
16	2.013551199573127E-4	0.0	0.0	17.195727244354504	0.0
17	2.013551199573127E-4	0.0	0.0	18.00839650850222	0.0
18	2.013551199573127E-4	0.0	0.0	18.92476365942795	0.0
19	2.013551199573127E-4	0.0	0.0	19.714277084780573	0.0
20	2.013551199573127E-4	0.0	0.0	20.32297361241153	0.0
21	2.013551199573127E-4	0.0	0.0	20.97697504203288	0.0
22	2.013551199573127E-4	0.0	0.0	21.70910225819767	0.0
23	2.013551199573127E-4	0.0	0.0	22.366124014618382	0.0
24	2.013551199573127E-4	0.0	0.0	22.90132592346492	0.0
25	2.013551199573127E-4	0.0	0.0	23.405720498957987	0.0
26	2.013551199573127E-4	0.0	0.0	23.929646521086916	0.0
27	2.013551199573127E-4	0.0	0.0	24.495253053047005	0.0
28	2.013551199573127E-4	0.0	0.0	24.975686369265155	0.0
29	2.013551199573127E-4	0.0	0.0	25.509680147391947	0.0
30	2.013551199573127E-4	0.0	0.0	26.17717237005044	0.0
31	2.013551199573127E-4	0.0	0.0	26.72485829633433	0.0
32	2.013551199573127E-4	0.0	0.0	27.24093146878492	0.0
33	2.013551199573127E-4	0.0	0.0	27.745527399397947	0.0
34	2.013551199573127E-4	0.0	0.0	28.318785425916417	0.0
35	2.013551199573127E-4	0.0	0.0	28.897480040673734	0.0
36	2.013551199573127E-4	0.0	0.0	29.411539661924753	0.0
37	2.013551199573127E-4	0.0	0.0	29.911907135018676	0.0
38	2.013551199573127E-4	0.0	0.0	30.50107221601377	0.0
39	4.027102399146254E-4	0.0	0.0	31.326024142478882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	50	2.1827873E-11	45.0	1
CAATCGT	20	7.029412E-4	45.0	41
CAATCGA	40	6.8012014E-9	45.0	41
AGTACGG	75	0.0	45.0	2
CCAATCG	60	0.0	44.999996	40
GCGGGTT	60	0.0	44.999996	5
CCGATGA	875	0.0	42.428574	18
GATGAAT	935	0.0	41.14973	20
CGTTTTT	1285	0.0	41.14786	1
CGATGAA	910	0.0	41.043957	19
ACGGGAC	50	1.0786607E-9	40.5	5
ATACGAA	50	1.0786607E-9	40.5	19
GATTACG	45	1.9244908E-8	40.0	1
ATGAATG	970	0.0	39.896908	21
TGCGTAG	40	3.452933E-7	39.375	1
TACGGCT	940	0.0	38.776596	7
GTTGGCG	35	6.2411236E-6	38.57143	1
GTAACGG	35	6.2411236E-6	38.57143	2
ACGACGG	35	6.2411236E-6	38.57143	2
CCACCGA	1160	0.0	38.405174	15
>>END_MODULE