##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935481.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465028
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239286666609324	31.0	31.0	33.0	28.0	34.0
2	31.267932253541723	31.0	31.0	34.0	28.0	34.0
3	31.042543674789474	31.0	31.0	34.0	27.0	34.0
4	34.82422348761795	35.0	35.0	37.0	32.0	37.0
5	34.88160067780865	35.0	35.0	37.0	33.0	37.0
6	35.06477674462613	37.0	35.0	37.0	32.0	37.0
7	35.475745546504726	37.0	35.0	37.0	33.0	37.0
8	35.26227022888944	37.0	35.0	37.0	32.0	37.0
9	37.17961714133342	39.0	37.0	39.0	34.0	39.0
10	36.45818316316437	38.0	35.0	39.0	32.0	39.0
11	36.778897184685654	39.0	37.0	39.0	33.0	39.0
12	36.897711535649464	39.0	37.0	39.0	33.0	39.0
13	36.98821791376003	39.0	37.0	39.0	33.0	39.0
14	38.05422039103022	40.0	37.0	41.0	33.0	41.0
15	38.22686590914956	40.0	37.0	41.0	34.0	41.0
16	38.15913235332066	40.0	37.0	41.0	34.0	41.0
17	38.07327515762492	40.0	37.0	41.0	33.0	41.0
18	37.99290795392965	39.0	37.0	41.0	34.0	41.0
19	37.819636667039404	39.0	37.0	41.0	33.0	41.0
20	37.87397533051773	39.0	36.0	41.0	34.0	41.0
21	37.79657999088227	39.0	36.0	41.0	33.0	41.0
22	37.918174819580756	40.0	36.0	41.0	34.0	41.0
23	37.97657130323336	40.0	36.0	41.0	34.0	41.0
24	37.95105456015552	40.0	36.0	41.0	34.0	41.0
25	37.535651616676844	39.0	35.0	41.0	33.0	41.0
26	37.626499909682856	39.0	35.0	41.0	33.0	41.0
27	37.70466939625141	40.0	36.0	41.0	33.0	41.0
28	37.626076279277804	40.0	35.0	41.0	33.0	41.0
29	37.58639479773261	40.0	35.0	41.0	33.0	41.0
30	37.28136155242265	39.0	35.0	41.0	33.0	41.0
31	37.24630559880265	39.0	35.0	41.0	33.0	41.0
32	37.15602501354757	39.0	35.0	41.0	32.0	41.0
33	37.064916521155716	40.0	35.0	41.0	32.0	41.0
34	36.902053209699204	40.0	35.0	41.0	31.0	41.0
35	36.85487325494379	40.0	35.0	41.0	31.0	41.0
36	36.693966814901465	40.0	35.0	41.0	31.0	41.0
37	36.71365810230782	40.0	35.0	41.0	31.0	41.0
38	36.55051953860843	39.0	35.0	41.0	31.0	41.0
39	36.47108991286546	39.0	35.0	41.0	30.0	41.0
40	36.38242428412913	39.0	35.0	41.0	30.0	41.0
41	36.23896625579535	39.0	35.0	41.0	30.0	41.0
42	36.265936674780875	39.0	35.0	41.0	30.0	41.0
43	36.237630852335776	39.0	35.0	41.0	30.0	41.0
44	36.20647358868713	39.0	35.0	41.0	30.0	41.0
45	36.16536208572387	39.0	35.0	41.0	30.0	41.0
46	36.04987871698048	39.0	35.0	41.0	30.0	41.0
47	36.04541662007449	39.0	35.0	41.0	30.0	41.0
48	35.95416405033676	39.0	35.0	41.0	29.0	41.0
49	35.93728334637914	39.0	35.0	41.0	29.0	41.0
50	35.85663013840027	38.0	35.0	40.0	29.0	41.0
51	34.755281402410176	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	33.0
10	28.0
11	30.0
12	25.0
13	28.0
14	32.0
15	35.0
16	53.0
17	85.0
18	152.0
19	284.0
20	511.0
21	793.0
22	1210.0
23	1769.0
24	2611.0
25	4039.0
26	5216.0
27	6066.0
28	6039.0
29	6383.0
30	7461.0
31	9075.0
32	11889.0
33	17002.0
34	30278.0
35	34392.0
36	37216.0
37	57491.0
38	102419.0
39	122350.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28676122728094	20.27899395305229	22.87346138297049	15.560783436696285
2	37.53515917321108	22.48058181442838	23.357733297779916	16.626525714580627
3	32.38450157839958	22.37800734579423	28.680423544388724	16.557067531417463
4	27.814024101774514	24.53250126874081	28.412267648399663	19.24120698108501
5	24.038552517267778	29.29156953989867	27.39146890079737	19.27840904203618
6	24.219186801654953	33.91989299569058	27.66586098041408	14.195059222240381
7	76.0300455026364	5.985876119287441	13.095555536440816	4.888522841635343
8	76.69516674264776	5.565041244828269	12.533008765063608	5.2067832474603675
9	70.58026613451233	7.077208254126634	14.996086257171612	7.3464393541894255
10	37.26936872618423	23.17709901339274	23.597718847037168	15.955813413385862
11	29.025564052057078	24.487127656829266	27.19578175937793	19.291526531735723
12	24.93527271476126	21.762345493174603	32.6451310458725	20.65725074619163
13	23.688681111674995	21.734820268887034	34.65038664338491	19.926111976053054
14	20.674023929741864	25.234824569703328	33.20789285806446	20.883258642490347
15	19.717092304119323	24.818075470724345	35.37872988293178	20.086102342224553
16	22.515203385602586	24.252948209570178	33.285522592188	19.94632581263924
17	22.446820406513158	24.69098634920908	31.018132241499437	21.84406100277833
18	23.077105034535556	24.869040143819294	32.085164764272264	19.96869005737289
19	22.454561875844036	26.852146537412803	30.17216167628616	20.521129910457006
20	23.673628254642733	25.95435113584558	30.47300377611671	19.899016833394978
21	23.060546891800062	26.960097026415614	30.77599628409472	19.2033597976896
22	21.972655409996815	24.48046139157212	31.174896995449735	22.371986202981326
23	21.41268912839657	26.022734114935016	31.29402960681937	21.270547149849044
24	21.965989144739673	24.727113206086514	31.64562133893013	21.661276310243686
25	22.185760857410735	25.95413609503084	29.839278495058362	22.020824552500066
26	21.03701282503419	25.50986177176428	30.83513250815005	22.61799289505148
27	21.59611894337545	25.60469477106755	30.943298037967605	21.85588824758939
28	20.387159482869848	26.171972440369185	30.928890303379582	22.51197777338139
29	21.734175146442794	26.671082171396133	29.94894931057915	21.645793371581927
30	22.371771162166578	26.939883189829427	29.873685025417824	20.814660622586167
31	24.141987149160908	26.630224416594267	27.900040427673172	21.32774800657165
32	24.33186818858219	27.571458062740305	27.877031060495284	20.21964268818222
33	23.39213982813938	27.139871147543804	27.9430485906225	21.524940433694315
34	23.37579672621864	26.60893537593435	28.922774542608188	21.092493355238826
35	22.888944321632245	26.938807985755698	27.956596161951534	22.21565153066052
36	23.036677361363186	28.799556155758367	27.460066920701546	20.7036995621769
37	23.66524166286761	27.29964647290056	28.112285711828104	20.922826152403726
38	23.244191747593693	27.369749778507963	27.764134632753297	21.621923841145048
39	23.548474500460188	25.662110668604903	28.49161770904118	22.297797121893737
40	23.494929337588275	24.995699183705067	30.144206370369098	21.365165108337564
41	21.25334388466931	26.19820741976827	29.801001230033457	22.747447465528957
42	21.835889451817955	26.23928021538488	29.343179335437863	22.581650997359297
43	21.804063411235454	25.266005487841593	30.221836104492635	22.708094996430322
44	22.875611791117954	25.167516794687632	29.162760091865437	22.794111322328977
45	22.765510893967676	24.911833265953877	28.785363461985085	23.537292378093362
46	23.16699209509965	25.73070868850908	28.13464995656176	22.967649259829514
47	21.63676165736257	25.9949938498327	30.08399494224004	22.2842495505647
48	21.27377276207024	25.3518067729255	30.144206370369098	23.230214094635162
49	22.160386041270634	24.304342964294623	30.5536440816467	22.981626912788048
50	20.61037184857686	24.46325812639239	31.30091091289127	23.625459112139485
51	20.782189459559426	23.87641174294881	29.994537963305433	25.34686083418633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	368.0
2	560.0
3	4770.0
4	8980.0
5	5627.5
6	2275.0
7	2145.5
8	2016.0
9	2109.5
10	2203.0
11	2133.0
12	2063.0
13	2024.5
14	1986.0
15	1943.0
16	1900.0
17	1827.5
18	1755.0
19	1753.5
20	1752.0
21	1852.0
22	1952.0
23	2065.5
24	2179.0
25	2551.5
26	3253.5
27	3583.0
28	4411.0
29	5239.0
30	6129.0
31	7019.0
32	8245.0
33	9471.0
34	10879.5
35	12288.0
36	13512.0
37	14736.0
38	15860.0
39	16984.0
40	18502.5
41	20021.0
42	22132.5
43	24244.0
44	26271.0
45	28298.0
46	34066.5
47	39835.0
48	42023.5
49	44212.0
50	43544.0
51	42876.0
52	38341.0
53	33806.0
54	30130.0
55	26454.0
56	24614.5
57	22775.0
58	20973.0
59	19171.0
60	18217.5
61	17264.0
62	15960.0
63	14656.0
64	12567.0
65	10478.0
66	8686.0
67	6894.0
68	5798.5
69	4703.0
70	3911.5
71	3120.0
72	2567.5
73	2015.0
74	1557.0
75	884.0
76	669.0
77	450.5
78	232.0
79	159.5
80	87.0
81	63.0
82	39.0
83	26.5
84	14.0
85	10.5
86	7.0
87	8.0
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	465028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.74597775436704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.17105876779907	29.40680355061857
2	9.340158476556264	7.611477790160485
3	4.128525659555873	5.046624440477912
4	2.6080057333578823	4.2506297437864795
5	2.0284965486770954	4.132653762360363
6	1.5293053160448704	3.7387824232319726
7	1.3054361516886617	3.723389067751691
8	1.0873603691888354	3.54444491311589
9	0.9280622336185879	3.4033322813122044
>10	4.810677695563099	29.56026756222211
>50	0.03572181536134124	0.935443341226428
>100	0.020793295508836903	1.441399574787853
>500	0.004798452809731593	1.362106305036901
>1k	0.0010663228466070208	0.5144286651239843
>5k	5.331614233035104E-4	1.3282165787871791
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6114	1.3147595413609503	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	1314	0.2825636305770835	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	1054	0.2266530187429574	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	934	0.20084812097336074	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	850	0.18278469253464308	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	790	0.16988224364984475	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	671	0.14429238669499472	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	649	0.13956148877056868	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	648	0.13934644795582202	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	631	0.13569075410512915	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	570	0.12257326440558419	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	527	0.1133265093714787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1260139174415304	0.0
2	2.1504081474663892E-4	0.0	0.0	0.5442683021237431	0.0
3	2.1504081474663892E-4	0.0	0.0	0.9139234626732153	0.0
4	2.1504081474663892E-4	0.0	0.0	1.390023826522274	0.0
5	2.1504081474663892E-4	0.0	0.0	2.622852817464755	0.0
6	2.1504081474663892E-4	0.0	0.0	3.7728910947297796	0.0
7	2.1504081474663892E-4	0.0	0.0	4.647032006674867	0.0
8	2.1504081474663892E-4	0.0	0.0	5.931470793156541	0.0
9	2.1504081474663892E-4	0.0	0.0	6.592506257687709	0.0
10	2.1504081474663892E-4	0.0	0.0	7.723835984069776	0.0
11	2.1504081474663892E-4	0.0	0.0	8.817963649500674	0.0
12	2.1504081474663892E-4	0.0	0.0	9.851449805173022	0.0
13	2.1504081474663892E-4	0.0	0.0	10.320883903764935	0.0
14	2.1504081474663892E-4	0.0	0.0	10.567535718279329	0.0
15	2.1504081474663892E-4	0.0	0.0	10.88235547106841	0.0
16	2.1504081474663892E-4	0.0	0.0	11.41845222223178	0.0
17	2.1504081474663892E-4	0.0	0.0	12.007879095452317	0.0
18	2.1504081474663892E-4	0.0	0.0	12.675365784425884	0.0
19	2.1504081474663892E-4	0.0	0.0	13.105232373104416	0.0
20	2.1504081474663892E-4	0.0	0.0	13.50348796201519	0.0
21	2.1504081474663892E-4	0.0	0.0	13.9955013461555	0.0
22	2.1504081474663892E-4	0.0	0.0	14.531813138133618	0.0
23	2.1504081474663892E-4	0.0	0.0	15.070490379073949	0.0
24	2.1504081474663892E-4	0.0	0.0	15.514549661525757	0.0
25	2.1504081474663892E-4	0.0	0.0	15.929578433986771	0.0
26	2.1504081474663892E-4	0.0	0.0	16.295792941500295	0.0
27	2.1504081474663892E-4	0.0	0.0	16.661147285754836	0.0
28	2.1504081474663892E-4	0.0	0.0	17.049080915557774	0.0
29	2.1504081474663892E-4	0.0	0.0	17.469270667572705	0.0
30	2.1504081474663892E-4	0.0	0.0	17.98214301074344	0.0
31	2.1504081474663892E-4	0.0	0.0	18.405773415794318	0.0
32	2.1504081474663892E-4	0.0	0.0	18.839940820767783	0.0
33	2.1504081474663892E-4	0.0	0.0	19.253679348340317	0.0
34	2.1504081474663892E-4	0.0	0.0	19.667417875912847	0.0
35	2.1504081474663892E-4	0.0	0.0	20.126314974582176	0.0
36	2.1504081474663892E-4	0.0	0.0	20.535967726674524	0.0
37	2.1504081474663892E-4	0.0	0.0	20.956802601133695	0.0
38	2.1504081474663892E-4	0.0	0.0	21.422796046689662	0.0
39	2.1504081474663892E-4	0.0	0.0	21.927496838900023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGAT	35	1.2098462E-7	45.0	5
TGCGACG	20	7.02895E-4	45.0	1
TATTACG	20	7.02895E-4	45.0	1
GGACGTA	20	7.02895E-4	45.0	8
CGTCGGT	20	7.02895E-4	45.0	26
AATAGCG	40	6.7993824E-9	45.0	10
CGTCATA	20	7.02895E-4	45.0	38
GCCGCAT	30	2.1627038E-6	44.999996	19
TACGCGG	30	2.1627038E-6	44.999996	2
CGTTTTT	2475	0.0	43.272728	1
CGTAAGG	70	0.0	41.785713	2
AGCGAAT	45	1.924127E-8	40.0	13
CTCGTAA	35	6.2402687E-6	38.571426	33
ATTGCGG	35	6.2402687E-6	38.571426	2
TACGGGT	65	9.094947E-12	38.07692	4
ACAACGG	30	1.138877E-4	37.499996	2
CGCGGGT	85	0.0	37.058823	4
GTTTTTT	3055	0.0	36.30933	2
TCGTTCA	50	4.8683432E-8	36.0	16
TCCATCG	25	0.0021058216	36.0	30
>>END_MODULE