SRR2935480.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935480.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659402
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10241	1.5530738456965554	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3587	0.5439777252722922	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	2203	0.33409058510589895	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	2190	0.33211910185289095	RNA PCR Primer, Index 41 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	2163	0.3280244827889512	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	1748	0.26508867125061797	Illumina PCR Primer Index 11 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1647	0.24977176290032482	Illumina PCR Primer Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1513	0.2294503201385498	RNA PCR Primer, Index 41 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT	1220	0.18501612066690729	RNA PCR Primer, Index 41 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1114	0.16894094952699568	RNA PCR Primer, Index 41 (96% over 26bp)
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1038	0.1574153551247949	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	967	0.14664802351221257	RNA PCR Primer, Index 41 (96% over 27bp)
CGTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	918	0.13921704817395156	RNA PCR Primer, Index 41 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.031097E-4	45.0	1
AATTGCG	35	1.210883E-7	45.0	1
CGTAAGC	25	3.888991E-5	45.0	43
ATACGGA	20	7.031097E-4	45.0	4
CGTTTAC	20	7.031097E-4	45.0	33
CGAACGG	30	2.164088E-6	44.999996	2
CGTTTTT	4650	0.0	43.887096	1
CGAATAT	75	0.0	42.0	14
CGTAAGG	70	0.0	41.785713	2
TCACGAC	65	0.0	41.53846	25
GCGATGC	55	6.002665E-11	40.909092	9
GCTACGA	80	0.0	39.375	10
TTCGTAG	35	6.2442396E-6	38.571426	1
GCGTAAG	35	6.2442396E-6	38.571426	1
CCGATGA	275	0.0	37.636364	18
TCGCACG	30	1.1393943E-4	37.499996	1
GATCGCG	30	1.1393943E-4	37.499996	9
TACGACG	30	1.1393943E-4	37.499996	1
TTACGGG	120	0.0	37.499996	3
CGATGTA	30	1.1393943E-4	37.499996	10