##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935480.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659402
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22390590262086	31.0	31.0	33.0	28.0	34.0
2	31.271198752809365	31.0	31.0	34.0	28.0	34.0
3	31.04183942420557	31.0	31.0	34.0	27.0	34.0
4	34.793141361415344	35.0	35.0	37.0	32.0	37.0
5	34.86155031376914	35.0	35.0	37.0	33.0	37.0
6	35.05004837716598	37.0	35.0	37.0	32.0	37.0
7	35.429727237709315	37.0	35.0	37.0	33.0	37.0
8	35.24860858778105	37.0	35.0	37.0	32.0	37.0
9	37.19002520465513	39.0	37.0	39.0	34.0	39.0
10	36.51051558836643	38.0	35.0	39.0	32.0	39.0
11	36.78823236811535	39.0	37.0	39.0	33.0	39.0
12	36.92531263174816	39.0	37.0	39.0	33.0	39.0
13	37.01009551078098	39.0	37.0	39.0	33.0	39.0
14	38.097195640898875	40.0	37.0	41.0	33.0	41.0
15	38.262534842175185	40.0	38.0	41.0	34.0	41.0
16	38.2121437302283	40.0	37.0	41.0	34.0	41.0
17	38.11210460386835	40.0	37.0	41.0	33.0	41.0
18	38.00592658196366	39.0	37.0	41.0	34.0	41.0
19	37.81516434587702	39.0	37.0	41.0	34.0	41.0
20	37.79636397827122	39.0	36.0	41.0	34.0	41.0
21	37.757137527638676	39.0	36.0	41.0	33.0	41.0
22	37.865320396359124	40.0	36.0	41.0	33.0	41.0
23	37.91416920179193	40.0	36.0	41.0	34.0	41.0
24	37.880700998783745	40.0	36.0	41.0	34.0	41.0
25	37.480755290399486	39.0	35.0	41.0	33.0	41.0
26	37.57480262419586	39.0	35.0	41.0	33.0	41.0
27	37.646428127303224	39.0	35.0	41.0	33.0	41.0
28	37.537673528439406	40.0	35.0	41.0	33.0	41.0
29	37.514014212877726	40.0	35.0	41.0	33.0	41.0
30	37.198895362768084	39.0	35.0	41.0	32.0	41.0
31	37.10518773070145	39.0	35.0	41.0	32.0	41.0
32	36.954531530083315	39.0	35.0	41.0	31.0	41.0
33	36.82354618275347	40.0	35.0	41.0	31.0	41.0
34	36.61080797449811	40.0	35.0	41.0	30.0	41.0
35	36.5707535008993	40.0	35.0	41.0	30.0	41.0
36	36.41613765199378	40.0	35.0	41.0	30.0	41.0
37	36.41369452928563	40.0	35.0	41.0	30.0	41.0
38	36.266292792560535	39.0	35.0	41.0	30.0	41.0
39	36.17597307863792	39.0	35.0	41.0	29.0	41.0
40	36.09597180475643	39.0	35.0	41.0	29.0	41.0
41	35.92465749269793	39.0	35.0	41.0	28.0	41.0
42	35.96991061598236	39.0	35.0	41.0	28.0	41.0
43	35.951563689524754	39.0	35.0	41.0	28.0	41.0
44	35.93130139126057	39.0	35.0	41.0	28.0	41.0
45	35.91329265000713	39.0	35.0	41.0	28.0	41.0
46	35.80429844010179	39.0	35.0	41.0	27.0	41.0
47	35.75407717901978	39.0	35.0	41.0	27.0	41.0
48	35.68152659530908	39.0	35.0	41.0	27.0	41.0
49	35.67108228364488	39.0	35.0	41.0	27.0	41.0
50	35.56846506380023	39.0	35.0	41.0	27.0	41.0
51	34.445059311315404	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	19.0
9	31.0
10	30.0
11	33.0
12	29.0
13	30.0
14	37.0
15	52.0
16	68.0
17	126.0
18	251.0
19	426.0
20	787.0
21	1355.0
22	2047.0
23	3063.0
24	4603.0
25	7296.0
26	9222.0
27	10005.0
28	9703.0
29	9862.0
30	10860.0
31	13200.0
32	16745.0
33	23449.0
34	39880.0
35	47219.0
36	50900.0
37	78310.0
38	143427.0
39	176305.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13606267496914	20.151591896900527	22.576667950658326	17.135677477472015
2	38.59360450832724	23.12534690522625	22.38240102395807	15.898647562488435
3	30.67764428982624	22.79656415964768	30.9110375764708	15.614753974055281
4	26.363735627128822	23.956857880321866	31.418921992957255	18.260484499592057
5	23.886794398561122	28.433944695345176	28.901186226308077	18.778074679785625
6	23.029199183502627	33.20933209180439	29.778041316222883	13.983427408470098
7	74.4031713582913	5.526825820971122	15.501924470960052	4.568078349777525
8	75.52464202413702	5.780995508051234	13.95112541363235	4.743237054179393
9	69.40121504029409	6.842108455843325	16.150390808641767	7.606285695220821
10	35.38842769660996	25.675081361597325	23.896045204594465	15.04044573719825
11	26.8051962232447	24.38103008483444	30.227691150466633	18.58608254145423
12	24.4005932648066	21.218012684219943	33.88919050897631	20.492203541997142
13	23.520098513501626	21.86056457214264	35.415573504478296	19.203763409877432
14	19.50358051689258	26.095765557277655	33.704477693425254	20.69617623240451
15	19.120809460693174	25.40150014710298	36.411172547247354	19.06651784495649
16	21.355409901698813	25.354791159262486	33.400262662230325	19.889536276808382
17	21.4526191913279	25.33932259835427	32.194321521621106	21.013736688696728
18	21.720589261179068	24.942447854267957	32.82549946769952	20.511463416853452
19	21.558927634432408	26.57908225938047	31.15686637286511	20.705123733322008
20	22.5445176083785	25.913934140327143	32.34688399489234	19.194664256402014
21	21.950646191549314	27.173105328767583	32.428776376171136	18.44747210351197
22	20.839336247084482	24.12200751590077	33.14427314445513	21.89438309255962
23	20.475066802951766	25.709809797361853	32.955162404724284	20.8599609949621
24	20.69693449519413	25.030406337863703	32.5667802038817	21.70587896306047
25	21.036181267269434	26.86934525524642	30.823079092875062	21.271394384609085
26	19.979617896215053	25.453820279586655	31.956227005680905	22.610334818517384
27	20.97430702363657	24.91014585943021	31.77105923245607	22.34448788447715
28	19.412285677022513	25.71375276386787	32.572391348524874	22.301570210584742
29	20.2096748265853	24.899530180375553	32.7204042450584	22.170390747980743
30	20.605639655324065	24.857977379504458	32.45213087009138	22.084252095080085
31	21.919557417174957	25.505382149280713	30.029026299586597	22.546034133957736
32	22.037239802123743	26.64414120672973	30.448800579919382	20.869818411227143
33	21.08228364487824	25.654153308603856	28.96897491969997	24.294588126817935
34	20.203760376826278	26.015693006693947	31.30472761684071	22.475818999639067
35	19.310223505539867	26.48354114788854	30.98519567729549	23.221039669276102
36	20.56393520189505	26.734526131252256	29.821413947789054	22.880124719063634
37	20.320077888753747	27.208288722205875	30.024931680522656	22.44670170851772
38	20.140976217845864	27.314748817868313	29.218443377484448	23.325831586801375
39	21.115040597389758	26.64687095277236	29.035247087512627	23.20284136232526
40	21.094870807185906	25.488397062793254	31.42316826457912	21.99356386544172
41	19.202853494529894	26.475958519992354	30.589989111346338	23.731198874131408
42	20.01919921383314	25.75576052241273	31.287590877795335	22.937449385958793
43	21.1447644987428	24.530711159505127	30.32763018613835	23.996894155613724
44	21.2407605679085	24.607750658930364	29.552079004916575	24.599409768244563
45	20.507065492673664	24.494466198161362	29.586959093239024	25.411509215925943
46	21.40985316999342	25.367075016454304	29.467153572479305	23.755918241072973
47	19.66706197433432	25.486122274424407	32.07497095853516	22.77184479270612
48	19.750015923518582	24.825220426992942	31.45486364918517	23.969900000303305
49	20.295206869254265	24.02267509046075	32.03614790370669	23.645970136578292
50	19.36663825708748	23.772296717328732	32.4989915104898	24.36207351509398
51	19.219383623343575	23.575148392027927	30.841732357499673	26.363735627128822
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	270.0
1	523.0
2	776.0
3	8083.0
4	15390.0
5	9833.5
6	4277.0
7	4174.0
8	4071.0
9	4016.0
10	3961.0
11	3890.0
12	3819.0
13	3697.5
14	3576.0
15	3461.0
16	3346.0
17	3207.0
18	3068.0
19	3026.5
20	2985.0
21	2928.5
22	2872.0
23	3067.5
24	3263.0
25	3681.5
26	4785.0
27	5470.0
28	6864.0
29	8258.0
30	9235.0
31	10212.0
32	11524.5
33	12837.0
34	15265.0
35	17693.0
36	18961.0
37	20229.0
38	22758.0
39	25287.0
40	27004.5
41	28722.0
42	31450.0
43	34178.0
44	37340.0
45	40502.0
46	52824.0
47	65146.0
48	64903.5
49	64661.0
50	63640.0
51	62619.0
52	55154.5
53	47690.0
54	42533.5
55	37377.0
56	34153.5
57	30930.0
58	28034.5
59	25139.0
60	22727.5
61	20316.0
62	18228.5
63	16141.0
64	13756.0
65	11371.0
66	9474.5
67	7578.0
68	6138.5
69	4699.0
70	3852.5
71	3006.0
72	2351.0
73	1696.0
74	1309.0
75	750.5
76	579.0
77	393.5
78	208.0
79	150.0
80	92.0
81	57.0
82	22.0
83	26.0
84	30.0
85	18.0
86	6.0
87	6.0
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	659402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.741697515554634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15755171979991	28.34247739707032
2	9.021902461288462	6.990476323401509
3	3.5989364325545385	4.182867199432116
4	2.2497774982425014	3.4864079725684896
5	1.6929089591202946	3.279308340780545
6	1.3997169131167935	3.2536445553226003
7	1.1694490568280582	3.1714509133638304
8	1.002931562425216	3.108421697622425
9	0.9182769389554717	3.201804666407008
>10	5.7162398167475335	33.397273767851885
>50	0.0462301148277604	1.2459161174156423
>100	0.019756459161695085	1.3970058255509221
>500	0.0019756459161695077	0.5550671842480432
>1k	0.003951291832339015	2.820188288858715
>5k	0.0	0.0
>10k+	3.9512918323390155E-4	1.56768975010596
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10241	1.5530738456965554	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	3587	0.5439777252722922	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	2203	0.33409058510589895	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	2190	0.33211910185289095	RNA PCR Primer, Index 41 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	2163	0.3280244827889512	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	1748	0.26508867125061797	Illumina PCR Primer Index 11 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1647	0.24977176290032482	Illumina PCR Primer Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1513	0.2294503201385498	RNA PCR Primer, Index 41 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT	1220	0.18501612066690729	RNA PCR Primer, Index 41 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1114	0.16894094952699568	RNA PCR Primer, Index 41 (96% over 26bp)
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	1038	0.1574153551247949	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	967	0.14664802351221257	RNA PCR Primer, Index 41 (96% over 27bp)
CGTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	918	0.13921704817395156	RNA PCR Primer, Index 41 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.516525579236945E-4	0.0	0.0	0.15347238861877882	0.0
2	1.516525579236945E-4	0.0	0.0	0.6725790943915851	0.0
3	1.516525579236945E-4	0.0	0.0	1.0753682882369178	0.0
4	1.516525579236945E-4	0.0	0.0	1.67682233296229	0.0
5	1.516525579236945E-4	0.0	0.0	3.332413307815263	0.0
6	1.516525579236945E-4	0.0	0.0	4.710176796552028	0.0
7	1.516525579236945E-4	0.0	0.0	5.6493610877734675	0.0
8	1.516525579236945E-4	0.0	0.0	6.8302795563252765	0.0
9	1.516525579236945E-4	0.0	0.0	7.413535294099805	0.0
10	1.516525579236945E-4	0.0	0.0	8.652233387220543	0.0
11	1.516525579236945E-4	0.0	0.0	9.769002823770629	0.0
12	1.516525579236945E-4	0.0	0.0	10.974034049032305	0.0
13	1.516525579236945E-4	0.0	0.0	11.440820622321437	0.0
14	1.516525579236945E-4	0.0	0.0	11.678611833145789	0.0
15	1.516525579236945E-4	0.0	0.0	12.013309028483384	0.0
16	1.516525579236945E-4	0.0	0.0	12.535600437972587	0.0
17	1.516525579236945E-4	0.0	0.0	13.129168549685927	0.0
18	1.516525579236945E-4	0.0	0.0	13.779454718062729	0.0
19	1.516525579236945E-4	0.0	0.0	14.251245825763343	0.0
20	3.03305115847389E-4	0.0	0.0	14.682394047940406	0.0
21	3.03305115847389E-4	0.0	0.0	15.176933039329574	0.0
22	3.03305115847389E-4	0.0	0.0	15.705593856251573	0.0
23	3.03305115847389E-4	0.0	0.0	16.233193105268107	0.0
24	3.03305115847389E-4	0.0	0.0	16.66267314930801	0.0
25	3.03305115847389E-4	0.0	0.0	17.063945817574105	0.0
26	3.03305115847389E-4	0.0	0.0	17.441560686804106	0.0
27	3.03305115847389E-4	0.0	0.0	17.842681702512277	0.0
28	3.03305115847389E-4	0.0	0.0	18.250172125653243	0.0
29	3.03305115847389E-4	0.0	0.0	18.682533568293696	0.0
30	3.03305115847389E-4	0.0	0.0	19.202398536856123	0.0
31	3.03305115847389E-4	0.0	0.0	19.63900625111844	0.0
32	3.03305115847389E-4	0.0	0.0	20.06514993888402	0.0
33	3.03305115847389E-4	0.0	0.0	20.495236593155617	0.0
34	3.03305115847389E-4	0.0	0.0	20.919257145110265	0.0
35	3.03305115847389E-4	0.0	0.0	21.367542106332706	0.0
36	3.03305115847389E-4	0.0	0.0	21.786103166202103	0.0
37	3.03305115847389E-4	0.0	0.0	22.21285346419938	0.0
38	4.549576737710835E-4	0.0	0.0	22.667811137970464	0.0
39	4.549576737710835E-4	0.0	0.0	23.15689063727438	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.031097E-4	45.0	1
AATTGCG	35	1.210883E-7	45.0	1
CGTAAGC	25	3.888991E-5	45.0	43
ATACGGA	20	7.031097E-4	45.0	4
CGTTTAC	20	7.031097E-4	45.0	33
CGAACGG	30	2.164088E-6	44.999996	2
CGTTTTT	4650	0.0	43.887096	1
CGAATAT	75	0.0	42.0	14
CGTAAGG	70	0.0	41.785713	2
TCACGAC	65	0.0	41.53846	25
GCGATGC	55	6.002665E-11	40.909092	9
GCTACGA	80	0.0	39.375	10
TTCGTAG	35	6.2442396E-6	38.571426	1
GCGTAAG	35	6.2442396E-6	38.571426	1
CCGATGA	275	0.0	37.636364	18
TCGCACG	30	1.1393943E-4	37.499996	1
GATCGCG	30	1.1393943E-4	37.499996	9
TACGACG	30	1.1393943E-4	37.499996	1
TTACGGG	120	0.0	37.499996	3
CGATGTA	30	1.1393943E-4	37.499996	10
>>END_MODULE