SRR2935479.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935479.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467051
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	2762	0.5913701073330322	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	2354	0.5040134803265597	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	2120	0.4539118854257886	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2053	0.4395665569712943	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1766	0.3781171649348786	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1117	0.23916017736821032	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	884	0.18927269184735715	TruSeq Adapter, Index 20 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT	710	0.15201765974165563	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	591	0.12653864353143449	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	531	0.11369208073636497	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACCTTAT	492	0.10534181491956982	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	30	2.1627238E-6	45.000004	8
ACGGGTC	30	2.1627238E-6	45.000004	5
ACGTAAG	30	2.1627238E-6	45.000004	1
TTGGCCG	20	7.028982E-4	45.0	1
TAGCGTG	20	7.028982E-4	45.0	1
AGGCGTC	25	3.887237E-5	45.0	35
TCGATCA	40	6.7993824E-9	45.0	17
TGCGAAG	55	1.8189894E-12	45.0	1
TCCGTAT	20	7.028982E-4	45.0	18
GTATACG	20	7.028982E-4	45.0	1
CGTCGAG	20	7.028982E-4	45.0	2
CCGATGA	225	0.0	41.0	18
CTCGATC	45	1.924127E-8	40.0	16
CGGCGAA	40	3.452351E-7	39.375	31
CGATGAA	235	0.0	39.255318	19
CGTTTTT	855	0.0	38.94737	1
CGTAAGG	110	0.0	38.863636	2
CCCACGA	35	6.2403287E-6	38.571426	39
CGTTAGG	35	6.2403287E-6	38.571426	2
ACGGACG	35	6.2403287E-6	38.571426	30