##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935479.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467051
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151756446298155	31.0	31.0	33.0	28.0	34.0
2	31.152908354762115	31.0	31.0	34.0	28.0	34.0
3	30.94845744897238	31.0	30.0	34.0	27.0	34.0
4	34.81625561234212	35.0	35.0	37.0	32.0	37.0
5	34.81193702614918	35.0	35.0	37.0	32.0	37.0
6	34.99030298618352	36.0	35.0	37.0	32.0	37.0
7	35.444795108028885	37.0	35.0	37.0	33.0	37.0
8	35.12520688318835	37.0	35.0	37.0	32.0	37.0
9	36.97777544636453	39.0	37.0	39.0	32.0	39.0
10	36.26730057317081	37.0	35.0	39.0	32.0	39.0
11	36.600525424418315	38.0	35.0	39.0	32.0	39.0
12	36.741826909695085	39.0	35.0	39.0	33.0	39.0
13	36.80994366782215	39.0	37.0	39.0	33.0	39.0
14	37.86393348906222	40.0	37.0	41.0	33.0	41.0
15	38.03502187127316	40.0	37.0	41.0	33.0	41.0
16	37.98769941612372	39.0	37.0	41.0	33.0	41.0
17	37.891371606098694	39.0	37.0	41.0	33.0	41.0
18	37.86668265350037	39.0	37.0	41.0	33.0	41.0
19	37.76635528025847	39.0	37.0	41.0	33.0	41.0
20	37.82863113450137	39.0	36.0	41.0	33.0	41.0
21	37.803783740961904	39.0	36.0	41.0	33.0	41.0
22	37.922220485557254	40.0	37.0	41.0	33.0	41.0
23	37.95034589370326	40.0	37.0	41.0	34.0	41.0
24	37.95143785154084	40.0	36.0	41.0	33.0	41.0
25	37.53299532599224	39.0	36.0	41.0	33.0	41.0
26	37.65148345683876	39.0	36.0	41.0	33.0	41.0
27	37.677938811821406	40.0	36.0	41.0	33.0	41.0
28	37.560966575384704	40.0	36.0	41.0	33.0	41.0
29	37.527064496168514	40.0	36.0	41.0	33.0	41.0
30	37.272556958447794	39.0	36.0	41.0	32.0	41.0
31	37.27950266673233	39.0	36.0	41.0	32.0	41.0
32	37.30309752039927	39.0	36.0	41.0	32.0	41.0
33	37.282448811799995	40.0	36.0	41.0	32.0	41.0
34	37.20043635491627	40.0	36.0	41.0	32.0	41.0
35	37.245264435789665	40.0	36.0	41.0	32.0	41.0
36	37.1135957315154	40.0	35.0	41.0	32.0	41.0
37	37.113462983699854	40.0	35.0	41.0	32.0	41.0
38	36.954756546929566	39.0	35.0	41.0	31.0	41.0
39	36.85599003106727	39.0	35.0	41.0	31.0	41.0
40	36.79784862895058	39.0	35.0	41.0	31.0	41.0
41	36.579095216582346	39.0	35.0	41.0	30.0	41.0
42	36.63785967699459	39.0	35.0	41.0	31.0	41.0
43	36.58965723229369	39.0	35.0	41.0	31.0	41.0
44	36.58358937246682	39.0	35.0	41.0	31.0	41.0
45	36.568927162130045	39.0	35.0	41.0	31.0	41.0
46	36.41163598836101	39.0	35.0	41.0	30.0	41.0
47	36.347407456573265	39.0	35.0	41.0	30.0	41.0
48	36.267336971765396	39.0	35.0	40.0	30.0	41.0
49	36.21576658651839	39.0	35.0	40.0	30.0	41.0
50	36.044205022577835	38.0	35.0	40.0	30.0	41.0
51	34.79095002472963	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	17.0
9	21.0
10	30.0
11	16.0
12	18.0
13	16.0
14	20.0
15	31.0
16	49.0
17	73.0
18	144.0
19	284.0
20	543.0
21	910.0
22	1364.0
23	1874.0
24	2483.0
25	3236.0
26	3861.0
27	4506.0
28	5018.0
29	5783.0
30	7438.0
31	9518.0
32	12683.0
33	17969.0
34	31208.0
35	34746.0
36	38864.0
37	59461.0
38	105350.0
39	119500.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.259725383309316	19.7751423292103	23.28803492552205	11.67709736195833
2	32.632838812035516	25.153569952746057	24.140190257594995	18.07340097762343
3	32.04789198610002	25.278823939997984	26.00829459737802	16.664989476523978
4	29.013105635144772	24.92425880685407	26.814630522148548	19.248005035852618
5	26.014503769395635	30.08086911279496	23.99802162932956	19.906605488479844
6	24.23418427537892	36.395811164091285	24.651911675598594	14.718092884931197
7	77.16566285052382	6.417286334897046	11.362784792238962	5.0542660223401725
8	77.2050589764287	7.660619504079855	9.450359810812952	5.6839617086784955
9	70.80297440750581	8.210880610468664	11.99890375997482	8.987241222050697
10	38.17634476748792	25.509633851549403	20.848472650738355	15.46554873022432
11	30.80263183249795	25.707256809213554	24.34338005913701	19.146731299151483
12	27.276464454631295	21.752228343371492	29.714313854375646	21.256993347621567
13	25.65351535485418	23.16320915702996	31.10067208934356	20.082603398772296
14	20.408691984387143	28.09885858289566	29.475581895767274	22.016867536949928
15	19.437920055839726	25.668288902068507	34.309529366172	20.58426167591976
16	21.859283033330406	25.68541765246194	30.34850583769224	22.106793476515413
17	22.113216757912948	25.775985920167177	28.361142573294995	23.74965474862488
18	22.630719129174327	26.150035006883616	29.267681687867064	21.95156417607499
19	23.04523488869524	27.612402071722357	27.345193565584914	21.997169473997484
20	25.275826408679137	26.222189867915922	28.25815596155452	20.24382776185042
21	23.570445197633664	28.280423337065976	28.13118909926325	20.017942366037115
22	21.01697673273368	26.039340457466103	28.730481253653238	24.213201556146974
23	21.758865734148948	27.49314314710813	28.64783503300496	22.10015608573796
24	22.85831740002698	25.31393787830451	28.441219481384262	23.38652524028425
25	21.199826143183508	28.436937293785903	26.951232306536117	23.412004256494473
26	21.08420707802788	26.391336278051003	28.7011482686045	23.823308375316614
27	23.457181335657136	26.5354318907357	27.769558356582042	22.23782841702512
28	20.46478864192561	27.540247210690055	29.0831194023779	22.911844745006434
29	23.062149529708748	25.48651003851828	28.000154158753542	23.451186273019434
30	24.148754632791707	25.899955251139595	27.754784809367717	22.19650530670098
31	23.08270403018086	27.903162609650767	25.370890973362652	23.643242386805724
32	23.93464525287388	27.368959706755792	25.8267298432077	22.86966519716262
33	22.96622852750556	26.594954298352857	25.632318526242315	24.806498647899268
34	21.89932148737504	25.380311786079034	29.022098229101317	23.698268497444605
35	22.267161402073864	24.772455256492332	28.584672765929202	24.375710575504602
36	22.68595934919313	29.02274055724107	26.497748639870167	21.793551453695635
37	21.371113647117767	28.173796865866898	28.65297365812299	21.802115828892347
38	20.01023442836007	28.28277854024507	26.482975092655835	25.22401193873902
39	21.892898205977506	25.44582925633389	27.50213574106468	25.159136796623923
40	22.675682098957072	23.480519258068178	30.533710451321163	23.310088191653588
41	20.18173604167425	26.529436828098003	28.90840614836495	24.380420981862795
42	21.873200143025066	26.20677399256184	29.032161369957453	22.887864494455638
43	22.52066690789657	26.255590931183104	27.253554750980086	23.97018740994024
44	21.720326045763738	25.38009767669912	26.992127198100423	25.907449079436724
45	21.764646687406728	23.36297320849329	27.55031035154619	27.32206975255379
46	22.963873324326464	26.00936514427761	27.004545542135656	24.022215989260275
47	20.062905335819856	25.725884325266406	30.891273115783928	23.31993722312981
48	20.675044053004918	24.802644679060744	29.452886301495983	25.06942496643835
49	20.97800882558864	24.268441776165773	30.455988746410988	24.297560651834598
50	20.411475406326076	23.14843560981563	30.856159177477405	25.58392980638089
51	20.160753322442304	23.007123419069867	28.556410327780053	28.27571293070778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	403.0
2	653.0
3	1774.5
4	2896.0
5	1939.5
6	983.0
7	915.5
8	848.0
9	881.0
10	914.0
11	893.5
12	873.0
13	885.0
14	897.0
15	869.5
16	842.0
17	857.0
18	872.0
19	878.0
20	884.0
21	1157.5
22	1431.0
23	1724.0
24	2017.0
25	2200.0
26	3137.0
27	3891.0
28	4441.0
29	4991.0
30	6173.5
31	7356.0
32	8194.5
33	9033.0
34	10440.5
35	11848.0
36	12981.0
37	14114.0
38	15066.5
39	16019.0
40	17921.5
41	19824.0
42	22100.0
43	24376.0
44	27332.0
45	30288.0
46	39008.0
47	47728.0
48	48301.5
49	48875.0
50	48169.0
51	47463.0
52	41499.0
53	35535.0
54	31958.5
55	28382.0
56	25984.5
57	23587.0
58	22285.0
59	20983.0
60	19704.5
61	18426.0
62	16172.5
63	13919.0
64	11672.5
65	9426.0
66	7611.0
67	5796.0
68	4498.5
69	3201.0
70	2668.5
71	2136.0
72	1866.5
73	1597.0
74	1189.5
75	603.0
76	424.0
77	309.0
78	194.0
79	153.5
80	113.0
81	82.5
82	52.0
83	39.0
84	26.0
85	17.5
86	9.0
87	8.5
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	467051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.29593309717847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31689307949951	28.077388322655
2	9.876679559227664	7.564733192449055
3	3.6308140594199996	4.171362369235299
4	2.0534092013844254	3.145488855893945
5	1.492493613641669	2.8578217787993743
6	1.131186070639573	2.599189564900397
7	0.999342833446523	2.6789536403568968
8	0.8553502364712664	2.6205148344447524
9	0.7231852259240591	2.49255477259701
>10	5.8224305975134865	36.881010684211354
>50	0.06321918711019282	1.6601401783062515
>100	0.02935176544401801	2.0325908979965686
>500	0.0022578281110783084	0.5871016568072615
>1k	0.003386742166617462	2.631149251346829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	2762	0.5913701073330322	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	2354	0.5040134803265597	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	2120	0.4539118854257886	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2053	0.4395665569712943	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1766	0.3781171649348786	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1117	0.23916017736821032	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	884	0.18927269184735715	TruSeq Adapter, Index 20 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT	710	0.15201765974165563	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	591	0.12653864353143449	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	531	0.11369208073636497	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACCTTAT	492	0.10534181491956982	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1410937991782483E-4	0.0	0.0	0.1984793951838236	0.0
2	2.1410937991782483E-4	0.0	0.0	1.0660506026108498	0.0
3	2.1410937991782483E-4	0.0	0.0	1.453160361502277	0.0
4	2.1410937991782483E-4	0.0	0.0	2.023975968363198	0.0
5	2.1410937991782483E-4	0.0	0.0	3.824421744092187	0.0
6	2.1410937991782483E-4	0.0	0.0	4.796692438299029	0.0
7	2.1410937991782483E-4	0.0	0.0	5.644779692153533	0.0
8	2.1410937991782483E-4	0.0	0.0	6.9403555500362915	0.0
9	2.1410937991782483E-4	0.0	0.0	7.3824914195666	0.0
10	2.1410937991782483E-4	0.0	0.0	8.841218624946741	0.0
11	2.1410937991782483E-4	0.0	0.0	10.291809673890004	0.0
12	2.1410937991782483E-4	0.0	0.0	11.878360179081085	0.0
13	2.1410937991782483E-4	0.0	0.0	12.435472785627265	0.0
14	2.1410937991782483E-4	0.0	0.0	12.678058713074162	0.0
15	2.1410937991782483E-4	0.0	0.0	13.12618964524217	0.0
16	2.1410937991782483E-4	0.0	0.0	13.94901199226637	0.0
17	2.1410937991782483E-4	0.0	0.0	14.846558512881892	0.0
18	2.1410937991782483E-4	0.0	0.0	15.847305754617803	0.0
19	2.1410937991782483E-4	0.0	0.0	16.519395098179857	0.0
20	2.1410937991782483E-4	0.0	0.0	17.115903830630916	0.0
21	2.1410937991782483E-4	0.0	0.0	17.834454909635138	0.0
22	2.1410937991782483E-4	0.0	0.0	18.602893474160208	0.0
23	2.1410937991782483E-4	0.0	0.0	19.31652003742632	0.0
24	4.2821875983564965E-4	0.0	0.0	19.905534941580257	0.0
25	4.2821875983564965E-4	0.0	0.0	20.44166482889449	0.0
26	4.2821875983564965E-4	0.0	0.0	20.962164731474722	0.0
27	4.2821875983564965E-4	0.0	0.0	21.522917197479504	0.0
28	4.2821875983564965E-4	0.0	0.0	22.023933146487213	0.0
29	4.2821875983564965E-4	0.0	0.0	22.549785783565394	0.0
30	4.2821875983564965E-4	0.0	0.0	23.164922032069303	0.0
31	4.2821875983564965E-4	0.0	0.0	23.69676973178518	0.0
32	4.2821875983564965E-4	0.0	0.0	24.210204024828123	0.0
33	4.2821875983564965E-4	0.0	0.0	24.75361363105956	0.0
34	4.2821875983564965E-4	0.0	0.0	25.28439078387585	0.0
35	4.2821875983564965E-4	0.0	0.0	25.836364765304005	0.0
36	4.2821875983564965E-4	0.0	0.0	26.347872073927686	0.0
37	4.2821875983564965E-4	0.0	0.0	26.879505664263647	0.0
38	6.423281397534745E-4	0.0	0.0	27.3978644730447	0.0
39	6.423281397534745E-4	0.0	0.0	27.92136190694378	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	30	2.1627238E-6	45.000004	8
ACGGGTC	30	2.1627238E-6	45.000004	5
ACGTAAG	30	2.1627238E-6	45.000004	1
TTGGCCG	20	7.028982E-4	45.0	1
TAGCGTG	20	7.028982E-4	45.0	1
AGGCGTC	25	3.887237E-5	45.0	35
TCGATCA	40	6.7993824E-9	45.0	17
TGCGAAG	55	1.8189894E-12	45.0	1
TCCGTAT	20	7.028982E-4	45.0	18
GTATACG	20	7.028982E-4	45.0	1
CGTCGAG	20	7.028982E-4	45.0	2
CCGATGA	225	0.0	41.0	18
CTCGATC	45	1.924127E-8	40.0	16
CGGCGAA	40	3.452351E-7	39.375	31
CGATGAA	235	0.0	39.255318	19
CGTTTTT	855	0.0	38.94737	1
CGTAAGG	110	0.0	38.863636	2
CCCACGA	35	6.2403287E-6	38.571426	39
CGTTAGG	35	6.2403287E-6	38.571426	2
ACGGACG	35	6.2403287E-6	38.571426	30
>>END_MODULE