SRR2935478.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935478.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248174
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1435	0.5782233432994592	RNA PCR Primer, Index 27 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	1236	0.49803766712064923	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	1235	0.4976347240242733	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	1213	0.48876997590400284	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.4379991457606357	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	687	0.2768219072102638	RNA PCR Primer, Index 38 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	528	0.2127539548864909	RNA PCR Primer, Index 38 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	367	0.14788011636996623	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	329	0.1325682787076809	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	282	0.1136299531780122	No Hit
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	273	0.11000346531062884	RNA PCR Primer, Index 27 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	20	7.0225855E-4	45.000004	2
CGTTCAT	20	7.0225855E-4	45.000004	17
ACGGGCC	20	7.0225855E-4	45.000004	5
CTGCGAT	20	7.0225855E-4	45.000004	19
TCGGACG	20	7.0225855E-4	45.000004	1
CCCCGAT	20	7.0225855E-4	45.000004	40
CGGGCCC	20	7.0225855E-4	45.000004	6
TGTTACG	20	7.0225855E-4	45.000004	1
GCGATTC	20	7.0225855E-4	45.000004	9
CTCGAGG	20	7.0225855E-4	45.000004	2
GAGCACG	20	7.0225855E-4	45.000004	1
TAGTGCG	20	7.0225855E-4	45.000004	1
TTCCTCG	20	7.0225855E-4	45.000004	12
CCCGATC	20	7.0225855E-4	45.000004	41
CGGGTGC	35	1.2067903E-7	45.0	6
GCGACAG	30	2.158602E-6	44.999996	1
GCGATTG	30	2.158602E-6	44.999996	9
CGTTTTT	555	0.0	42.567566	1
GGCGATT	80	0.0	42.187504	8
GGCGATA	55	6.002665E-11	40.90909	8