##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935477.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427057
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.119487562550198	31.0	31.0	33.0	28.0	34.0
2	31.094781258707854	31.0	31.0	34.0	28.0	34.0
3	30.925059184137012	31.0	30.0	34.0	27.0	34.0
4	34.7822304750888	35.0	35.0	37.0	32.0	37.0
5	34.788939181420744	35.0	35.0	37.0	32.0	37.0
6	34.990600786311894	36.0	35.0	37.0	32.0	37.0
7	35.44062502195257	37.0	35.0	37.0	33.0	37.0
8	35.15721788894691	37.0	35.0	37.0	32.0	37.0
9	37.008158161556885	39.0	37.0	39.0	33.0	39.0
10	36.203331171248806	37.0	35.0	39.0	32.0	39.0
11	36.595264800717466	38.0	35.0	39.0	32.0	39.0
12	36.75529261901807	39.0	35.0	39.0	33.0	39.0
13	36.80139887649658	39.0	37.0	39.0	33.0	39.0
14	37.82950519485689	39.0	37.0	41.0	33.0	41.0
15	37.97381380003138	40.0	37.0	41.0	33.0	41.0
16	37.971027286755636	39.0	37.0	41.0	33.0	41.0
17	37.88297815045767	39.0	37.0	41.0	33.0	41.0
18	37.90237134621374	39.0	37.0	41.0	33.0	41.0
19	37.79254291581686	39.0	37.0	41.0	33.0	41.0
20	37.83597037397818	39.0	37.0	41.0	33.0	41.0
21	37.87713115579419	39.0	37.0	41.0	33.0	41.0
22	38.00928915812175	40.0	37.0	41.0	34.0	41.0
23	38.03446144191525	40.0	37.0	41.0	34.0	41.0
24	38.01084866891305	40.0	37.0	41.0	34.0	41.0
25	37.64545482219001	39.0	36.0	41.0	33.0	41.0
26	37.73399569612487	39.0	36.0	41.0	33.0	41.0
27	37.79622860648578	40.0	37.0	41.0	33.0	41.0
28	37.71974701269386	40.0	36.0	41.0	33.0	41.0
29	37.75335845097961	40.0	36.0	41.0	33.0	41.0
30	37.482108945644256	40.0	36.0	41.0	33.0	41.0
31	37.50161688018227	39.0	36.0	41.0	33.0	41.0
32	37.49600170469048	40.0	36.0	41.0	33.0	41.0
33	37.50898123669674	40.0	36.0	41.0	33.0	41.0
34	37.47311248849685	40.0	36.0	41.0	33.0	41.0
35	37.48367782286674	40.0	36.0	41.0	33.0	41.0
36	37.34351620509674	40.0	36.0	41.0	32.0	41.0
37	37.37921635753541	40.0	36.0	41.0	33.0	41.0
38	37.22604242525003	40.0	36.0	41.0	32.0	41.0
39	37.19653348382066	40.0	36.0	41.0	32.0	41.0
40	37.07417042689852	39.0	36.0	41.0	31.0	41.0
41	36.955294023982745	39.0	35.0	41.0	31.0	41.0
42	37.063836911700314	39.0	36.0	41.0	32.0	41.0
43	37.033892431221126	39.0	35.0	41.0	32.0	41.0
44	37.06777315440328	39.0	35.0	41.0	32.0	41.0
45	37.0711029206874	39.0	35.0	41.0	32.0	41.0
46	36.958359188586066	39.0	35.0	41.0	31.0	41.0
47	36.91799455342027	39.0	35.0	41.0	31.0	41.0
48	36.86335313552992	39.0	35.0	41.0	31.0	41.0
49	36.9102297819729	39.0	35.0	41.0	31.0	41.0
50	36.79837117761797	39.0	35.0	41.0	31.0	41.0
51	35.71391406767715	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	24.0
10	14.0
11	16.0
12	16.0
13	12.0
14	7.0
15	18.0
16	28.0
17	56.0
18	112.0
19	184.0
20	344.0
21	676.0
22	1072.0
23	1577.0
24	2006.0
25	2533.0
26	3066.0
27	3445.0
28	4023.0
29	4796.0
30	6285.0
31	8355.0
32	11154.0
33	15610.0
34	26487.0
35	30506.0
36	34582.0
37	53419.0
38	98859.0
39	117748.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.234476896526694	20.346464289310326	23.807594770721472	10.611464043441508
2	32.35516570387559	27.049550762544577	23.38423208143175	17.211051452148073
3	31.03801131933208	26.17800434134085	26.600430387512674	16.183553951814396
4	27.574773390905666	23.982044551429905	29.402866596262328	19.04031546140211
5	26.6697419782371	29.493252657139447	24.773273825273908	19.063731539349547
6	22.692521138864365	36.69627239455155	25.944780204984347	14.66642626159974
7	76.21488466410807	5.889846086119651	12.990069241342491	4.905200008429788
8	75.93787246198985	7.977155274354478	9.937315159334702	6.1476571043209685
9	70.93198331838607	6.685056093214723	11.942433913974012	10.440526674425193
10	37.09879477446805	24.427418353990216	21.954212201181576	16.519574670360164
11	28.774379064153027	25.395907337896347	25.365934758123622	20.463778839827
12	24.41805192281124	21.375132593541377	31.631843056079166	22.574972427568216
13	24.027471742647936	23.81860032735677	33.914442334395645	18.23948559559965
14	20.11370847451277	28.016166460214915	30.06624408451331	21.803880980759008
15	18.69258670388262	26.067948774987883	35.634587420414604	19.604877100714894
16	20.011380213882457	25.81786506250922	30.52262344370892	23.648131279899403
17	20.571961119944177	26.502785342471846	29.742399726500206	23.182853811083767
18	20.417415005491073	25.597988090582756	32.12100492440119	21.863591979524983
19	21.166729499809158	27.920863022968827	28.64348318842684	22.268924288795173
20	22.148799808924803	27.86747436524867	30.761701599552282	19.222024226274247
21	20.98548905649597	29.55483694214121	29.951271141791374	19.50840285957144
22	19.087381778076463	25.556307471836316	29.919659436562334	25.436651313524894
23	18.813179505311936	28.254542133719855	30.0407205595506	22.89155780141761
24	21.223864730000912	25.51907590789988	29.273609845992453	23.98344951610675
25	18.816457756224576	29.932304118653953	27.44621912297422	23.805019002147255
26	17.601397471531904	26.89640961276832	30.48726516600829	25.014927749691495
27	20.953643190487455	26.522689008727173	29.707509770358527	22.816158030426852
28	16.844355671491158	26.538377780951954	32.5106484614466	24.106618086110284
29	19.19673486209101	24.304718105545632	30.615351112380786	25.883195919982576
30	19.62759069632391	27.32609464310385	31.325794917306123	21.72051974326612
31	19.4931824089056	25.9918465216587	28.974352369824167	25.540618699611528
32	19.868073816844124	27.30291272593588	29.30826564135467	23.520747815865327
33	18.89045256253849	26.240056947901568	29.838873967643664	25.030616521916276
34	17.93156417059081	24.038711460062707	30.6261225082366	27.403601861109877
35	17.934139939165032	23.425444378619247	30.518876871237328	28.121538810978393
36	18.07768049698284	25.450466799513883	31.951004198502776	24.520848505000505
37	17.31314555199891	24.215737009345357	35.4325066677282	23.038610770927534
38	16.905705795713452	27.0411209744835	31.211992778481562	24.841180451321488
39	19.574202038603747	24.81682773025615	31.018575974635702	24.5903942565044
40	18.99746403875829	24.42133017372388	29.930430832418153	26.65077495509967
41	17.1504038102642	23.922567713443403	29.203595772929607	29.723432703362786
42	17.93086168825239	22.729050220462373	33.30047277061376	26.039615320671476
43	19.92005750988744	23.142109835455223	32.4036369852268	24.534195669430545
44	18.95952999248344	23.713462137372762	31.318535933142417	26.00847193700138
45	18.792807517497664	22.936984992635644	29.602839901933464	28.667367587933228
46	21.346330817666026	24.829004090788818	29.038980744959105	24.785684346586052
47	16.101363518218882	24.10685224688976	34.52372868258804	25.26805555230332
48	17.897376696787546	23.22781268074285	31.591567402009566	27.283243220460037
49	18.601263999887603	21.414471604493077	35.261335137932406	24.722929257686914
50	18.623743434717145	21.561290413223528	32.48114420323282	27.333821948826504
51	17.68944192461428	21.13699108081591	29.294215994586203	31.879350999983608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97.0
1	244.0
2	391.0
3	1246.5
4	2102.0
5	1405.5
6	709.0
7	698.5
8	688.0
9	685.0
10	682.0
11	667.5
12	653.0
13	674.5
14	696.0
15	675.5
16	655.0
17	717.0
18	779.0
19	879.0
20	979.0
21	1082.5
22	1186.0
23	1374.0
24	1562.0
25	1790.5
26	2533.0
27	3047.0
28	3825.5
29	4604.0
30	5056.0
31	5508.0
32	7125.5
33	8743.0
34	9652.0
35	10561.0
36	11981.5
37	13402.0
38	14908.5
39	16415.0
40	19333.5
41	22252.0
42	25989.0
43	29726.0
44	42710.0
45	55694.0
46	54469.0
47	53244.0
48	52023.5
49	50803.0
50	42861.0
51	34919.0
52	30977.5
53	27036.0
54	23626.0
55	20216.0
56	18440.0
57	16664.0
58	14559.0
59	12454.0
60	11538.0
61	10622.0
62	9018.5
63	7415.0
64	6065.5
65	4716.0
66	3574.0
67	2432.0
68	1929.0
69	1426.0
70	1087.0
71	748.0
72	634.5
73	521.0
74	436.5
75	276.5
76	201.0
77	130.5
78	60.0
79	37.5
80	15.0
81	30.5
82	46.0
83	26.5
84	7.0
85	5.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	427057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.619618185689724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7286402925368	28.81480959554677
2	11.478620353739451	9.095571114272875
3	4.096473574975423	4.86902156844881
4	2.098291262698825	3.325339946819849
5	1.44903535519454	2.870511375518648
6	1.0606908091530816	2.5214498923029227
7	0.8137725468796474	2.2568950318157546
8	0.6732395604753575	2.1338795466828184
9	0.6054893054363331	2.1590329587215384
>10	4.892560590742159	32.68342796483
>50	0.06322400523611064	1.7305153650430245
>100	0.03220845549764127	2.153278165543089
>500	0.004175170157101647	1.245366103205891
>1k	0.0035787172775156974	4.140901371247974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC	4273	1.0005690106941227	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	3944	0.9235301142470443	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	3831	0.8970699461664369	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2939	0.6881985308752695	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1351	0.31635121306991804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	0.2774805236771672	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	983	0.23018004622333788	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT	934	0.21870616802909215	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTACCTT	739	0.17304481603158361	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	675	0.15805852614522184	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTC	656	0.1536094713352082	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	652	0.15267282821731057	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTA	631	0.1477554518483481	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	466	0.10911892323507166	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	466	0.10911892323507166	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	437	0.10232826063031397	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3416077947440272E-4	0.0	0.0	0.2404831205202116	0.0
2	2.3416077947440272E-4	0.0	0.0	1.5770728497601023	0.0
3	2.3416077947440272E-4	0.0	0.0	2.0587415731389487	0.0
4	2.3416077947440272E-4	0.0	0.0	2.863552172192471	0.0
5	2.3416077947440272E-4	0.0	0.0	5.456648644092007	0.0
6	2.3416077947440272E-4	0.0	0.0	6.522782673038962	0.0
7	2.3416077947440272E-4	0.0	0.0	7.692884088072552	0.0
8	2.3416077947440272E-4	0.0	0.0	9.461266294663242	0.0
9	2.3416077947440272E-4	0.0	0.0	9.968692703784273	0.0
10	2.3416077947440272E-4	0.0	0.0	12.237944817670709	0.0
11	2.3416077947440272E-4	0.0	0.0	14.259454826873228	0.0
12	2.3416077947440272E-4	0.0	0.0	16.753735449834565	0.0
13	2.3416077947440272E-4	0.0	0.0	17.39416518169706	0.0
14	2.3416077947440272E-4	0.0	0.0	17.659703505621028	0.0
15	2.3416077947440272E-4	0.0	0.0	18.261730869649718	0.0
16	2.3416077947440272E-4	0.0	0.0	19.31264444793084	0.0
17	2.3416077947440272E-4	0.0	0.0	20.637760298976485	0.0
18	2.3416077947440272E-4	0.0	0.0	22.005961733445417	0.0
19	4.6832155894880543E-4	0.0	0.0	22.99856927763741	0.0
20	4.6832155894880543E-4	0.0	0.0	23.803379876690933	0.0
21	4.6832155894880543E-4	0.0	0.0	24.7477503003112	0.0
22	4.6832155894880543E-4	0.0	0.0	25.792107376767035	0.0
23	4.6832155894880543E-4	0.0	0.0	26.838103578679192	0.0
24	4.6832155894880543E-4	0.0	0.0	27.652280608911692	0.0
25	7.024823384232081E-4	0.0	0.0	28.365768503970195	0.0
26	7.024823384232081E-4	0.0	0.0	29.016267149350085	0.0
27	7.024823384232081E-4	0.0	0.0	29.712895468286433	0.0
28	7.024823384232081E-4	0.0	0.0	30.39008844252641	0.0
29	7.024823384232081E-4	0.0	0.0	31.07524288326851	0.0
30	7.024823384232081E-4	0.0	0.0	31.83626541656032	0.0
31	7.024823384232081E-4	0.0	0.0	32.55115827629567	0.0
32	7.024823384232081E-4	0.0	0.0	33.22109226637193	0.0
33	7.024823384232081E-4	0.0	0.0	33.900158526847704	0.0
34	7.024823384232081E-4	0.0	0.0	34.596552685004575	0.0
35	7.024823384232081E-4	0.0	0.0	35.2641450672861	0.0
36	7.024823384232081E-4	0.0	0.0	35.91675115968126	0.0
37	9.366431178976109E-4	0.0	0.0	36.55273183673374	0.0
38	9.366431178976109E-4	0.0	0.0	37.229456489414765	0.0
39	9.366431178976109E-4	0.0	0.0	37.89330229922469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	20	7.028301E-4	45.000004	1
CCCTCGA	20	7.028301E-4	45.000004	41
CGAAAGG	40	6.7975634E-9	45.000004	2
GCGTTAG	20	7.028301E-4	45.000004	1
CGATGTC	20	7.028301E-4	45.000004	10
CAGTCGA	20	7.028301E-4	45.000004	21
TAGTACG	20	7.028301E-4	45.000004	1
TCAGTCG	20	7.028301E-4	45.000004	20
CGTGGTT	25	3.8866747E-5	45.0	14
TTTACGG	25	3.8866747E-5	45.0	2
GCGAAGT	25	3.8866747E-5	45.0	9
CCGTGGT	25	3.8866747E-5	45.0	13
GCACGAG	25	3.8866747E-5	45.0	1
AGTAACG	25	3.8866747E-5	45.0	1
AGCGACT	30	2.1622855E-6	44.999996	19
ACGGGTA	75	0.0	42.0	5
CGGGACC	125	0.0	41.399998	6
CGGGTCA	60	3.6379788E-12	41.249996	6
TAAGTAG	55	6.002665E-11	40.909092	1
CGTAAGG	55	6.002665E-11	40.909092	2
>>END_MODULE