FastQCFastQC Report
Sat 14 Jan 2017
SRR2935475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518229
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC66881.2905491587695788No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC63701.2291863249644461No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG56711.0943038695248626No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27310.5269871041566566No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC26590.513093632351721TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT14420.27825536587107247No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT12170.2348382664806485No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT11140.21496288320414333No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC9760.18833372891135No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA9030.1742472922202347No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC8560.16517794256979057TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT8110.1564945226917058TruSeq Adapter, Index 14 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT7690.14838999747216No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG6580.12697089510621754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC302.1631859E-645.00000416
CTTCGTC302.1631859E-645.00000436
CACCTCG207.029696E-445.00000416
TCGCTAT207.029696E-445.00000423
GCGTCTA207.029696E-445.00000424
CGACGGT253.887831E-545.028
TTAGCCG253.887831E-545.01
GCGAACA351.21021E-745.033
CGGGGTA551.8189894E-1245.06
TACGGCT7650.041.470597
CGTAAGG603.6379788E-1241.2500042
CCGATGA6700.040.9701518
CGTTTTT12000.040.68751
CGATGAA6750.040.66666819
ACGGGCG850.039.7058835
CGGGAAT1250.039.6000026
GATGAAT7250.039.4137920
CCGATTA403.453315E-739.37500418
TAGCACG403.453315E-739.3750041
CTCGAAT750.039.043
Produced by FastQC (version 0.11.5)