##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935475.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518229
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.156762743883494	31.0	31.0	33.0	28.0	34.0
2	31.150508751922413	31.0	31.0	34.0	28.0	34.0
3	30.983306607696598	31.0	30.0	34.0	27.0	34.0
4	34.84164915510325	35.0	35.0	37.0	32.0	37.0
5	34.81040235108417	35.0	35.0	37.0	33.0	37.0
6	34.97537768052347	36.0	35.0	37.0	32.0	37.0
7	35.41233508738415	37.0	35.0	37.0	33.0	37.0
8	35.050865543996956	37.0	35.0	37.0	32.0	37.0
9	36.882378253629184	39.0	37.0	39.0	32.0	39.0
10	36.225919429441426	37.0	35.0	39.0	32.0	39.0
11	36.59304091434482	38.0	35.0	39.0	32.0	39.0
12	36.758815118412905	39.0	35.0	39.0	33.0	39.0
13	36.7770290740194	39.0	35.0	39.0	33.0	39.0
14	37.85002962011003	40.0	37.0	41.0	33.0	41.0
15	37.96943050273142	40.0	37.0	41.0	33.0	41.0
16	38.0274974962806	40.0	37.0	41.0	33.0	41.0
17	37.84961667525361	39.0	37.0	41.0	33.0	41.0
18	37.85803573323762	39.0	37.0	41.0	33.0	41.0
19	37.77361552518288	39.0	37.0	41.0	33.0	41.0
20	37.69087025234018	39.0	36.0	41.0	33.0	41.0
21	37.77896837112551	39.0	36.0	41.0	33.0	41.0
22	37.879219418442425	40.0	37.0	41.0	33.0	41.0
23	37.88416510847521	40.0	37.0	41.0	33.0	41.0
24	37.85013961009515	40.0	36.0	41.0	33.0	41.0
25	37.54495985365543	39.0	36.0	41.0	33.0	41.0
26	37.57308448581612	39.0	36.0	41.0	33.0	41.0
27	37.586024711083326	40.0	36.0	41.0	33.0	41.0
28	37.49446287259107	40.0	36.0	41.0	33.0	41.0
29	37.498266210497675	40.0	36.0	41.0	33.0	41.0
30	37.259186575818795	39.0	36.0	41.0	32.0	41.0
31	37.257924585463186	39.0	36.0	41.0	32.0	41.0
32	37.176007131982196	39.0	36.0	41.0	32.0	41.0
33	37.15585387926959	40.0	36.0	41.0	32.0	41.0
34	37.072643561051194	40.0	36.0	41.0	31.0	41.0
35	37.06086112510106	40.0	36.0	41.0	32.0	41.0
36	36.951675803554025	40.0	35.0	41.0	31.0	41.0
37	36.931233103512156	40.0	35.0	41.0	31.0	41.0
38	36.77327590698321	39.0	35.0	41.0	31.0	41.0
39	36.672251070472704	39.0	35.0	41.0	30.0	41.0
40	36.53758859500337	39.0	35.0	41.0	30.0	41.0
41	36.3942600664957	39.0	35.0	41.0	30.0	41.0
42	36.4586350821741	39.0	35.0	41.0	30.0	41.0
43	36.4719998301909	39.0	35.0	41.0	30.0	41.0
44	36.50459738841323	39.0	35.0	41.0	30.0	41.0
45	36.51555586429937	39.0	35.0	41.0	30.0	41.0
46	36.36658311287095	39.0	35.0	41.0	30.0	41.0
47	36.22734351030143	39.0	35.0	41.0	30.0	41.0
48	36.16690304865224	39.0	35.0	40.0	30.0	41.0
49	36.18536785861077	39.0	35.0	40.0	30.0	41.0
50	36.05219893136046	39.0	35.0	40.0	30.0	41.0
51	34.84020963705235	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	31.0
10	30.0
11	18.0
12	27.0
13	15.0
14	18.0
15	21.0
16	43.0
17	91.0
18	177.0
19	362.0
20	818.0
21	1368.0
22	2169.0
23	2780.0
24	3366.0
25	4119.0
26	4685.0
27	5028.0
28	5541.0
29	6351.0
30	8172.0
31	10601.0
32	14081.0
33	19894.0
34	32754.0
35	37597.0
36	42635.0
37	64288.0
38	114884.0
39	136229.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.09153675305704	19.695347037699552	21.856746727797944	11.35636948144546
2	32.46653506461429	28.281512613149783	22.95452396527404	16.297428356961884
3	31.062908482543435	28.040499470311385	24.78672555954993	16.109866487595255
4	27.44693948042275	23.402781395869393	30.57123395255765	18.579045171150206
5	28.185030170059953	28.711245414671893	23.611569402715787	19.492155012552363
6	23.051006408363868	38.047851432474836	24.92816882111962	13.972973338041678
7	74.24748518512087	6.205943704424106	14.899783686362591	4.646787424092437
8	73.91191924805443	10.042278606561963	10.54089987245021	5.5049022729333945
9	67.9651273857696	7.602623550592498	12.319843158140513	12.112405905497377
10	38.637166194867525	23.852003650895647	21.619399917796958	15.891430236439874
11	30.850840072631986	25.166480455551504	24.56886820305309	19.41381126876342
12	25.50436197125209	21.30506011821029	30.17816447940968	23.012413431127936
13	25.346709659243306	22.73685957366338	32.720283889940546	19.196146877152763
14	20.690081025955706	28.94782036512816	28.809078611964985	21.553019996951157
15	18.503595900653956	25.70311580401714	34.98955095141337	20.803737343915525
16	20.913534364151758	25.286890544527612	30.177778549637324	23.62179654168331
17	20.86510017772066	26.337005455117335	29.701734175432097	23.09616019172991
18	20.64762875099618	26.04736516096166	29.98191919016497	23.323086897877193
19	21.84034471247267	27.404680170349398	27.772471243407836	22.982503873770092
20	23.955239865001765	26.262713973938162	30.563129427338108	19.218916733721965
21	22.489478589581054	29.418268757634173	28.977922887372188	19.114329765412588
22	20.382687962271508	24.971778885396226	30.166007691580365	24.47952546075191
23	21.83648541474908	28.174995995978612	28.788045439371395	21.200473149900912
24	22.352280555507313	25.96149578661171	27.994766792286807	23.69145686559417
25	19.97977727992837	29.88215634401008	27.024539344575466	23.11352703148608
26	19.54618518068267	25.736691694212404	30.061999617929523	24.6551235071754
27	23.24493611897443	26.870939295176456	27.626010894797474	22.25811369105164
28	18.701191944101932	27.64627220784634	30.490960559906917	23.161575288144817
29	20.741988580338035	25.02831759704687	29.425215493536644	24.804478329078457
30	20.10211701776628	27.655534522382965	30.905449135420827	21.336899324429933
31	21.81796078567583	27.511968647065295	26.135164184173405	24.53490638308547
32	22.744771133996746	30.346043930385985	26.0014395180509	20.907745417566364
33	21.725723570081954	26.11123653828713	28.476407148191246	23.686632743439677
34	22.476356977320837	26.91069006172947	27.147072047299552	23.465880913650143
35	21.158792734486106	26.93654735647754	28.048411030644754	23.8562488783916
36	20.033807448058678	28.784186141647805	27.00138355823391	24.18062285205961
37	20.290450746677628	28.234043251149586	30.08129610654749	21.394209895625295
38	19.10738302951012	29.674333161594586	26.905094080030256	24.31318972886504
39	22.71273896289092	26.479992435776463	27.624081245935677	23.18318735539694
40	21.40598075368225	25.124607075250516	31.06541702606377	22.403995145003464
41	20.268259784766965	26.743582470297877	27.58124304120379	25.406914703731363
42	20.0554195153108	27.17736753442976	29.11357720235649	23.653635747902953
43	21.049767573794597	25.25312168944617	28.03297383975038	25.66413689700885
44	21.07678265785975	25.472715729918626	29.103735993161322	24.3467656190603
45	20.589353355369923	24.78749741909465	27.763015963985033	26.860133261550395
46	22.8296756839158	27.095550422689584	27.111566508242497	22.963207385152124
47	19.806301847252854	25.463067485609642	32.20024352168636	22.53038714545114
48	20.440770393011583	26.053925967091768	28.430288540394304	25.075015099502345
49	20.091310984140215	24.197796726929603	31.80717404853839	23.903718240391797
50	20.429578429613162	23.870721244855073	30.0546669522547	25.645033373277066
51	19.549272618861547	23.66212620289486	28.320491520158075	28.468109658085517
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	391.0
2	616.0
3	2424.0
4	4232.0
5	2746.0
6	1260.0
7	1247.5
8	1235.0
9	1217.5
10	1200.0
11	1172.5
12	1145.0
13	1121.5
14	1098.0
15	1059.0
16	1020.0
17	1096.5
18	1173.0
19	1293.5
20	1414.0
21	1750.5
22	2087.0
23	2213.0
24	2339.0
25	2642.0
26	3568.0
27	4191.0
28	5283.0
29	6375.0
30	7450.5
31	8526.0
32	9336.0
33	10146.0
34	11237.5
35	12329.0
36	13792.0
37	15255.0
38	17243.5
39	19232.0
40	21966.5
41	24701.0
42	28283.5
43	31866.0
44	48193.5
45	64521.0
46	58056.0
47	51591.0
48	51586.0
49	51581.0
50	45024.0
51	38467.0
52	34645.5
53	30824.0
54	28198.0
55	25572.0
56	24045.0
57	22518.0
58	21328.5
59	20139.0
60	19410.0
61	18681.0
62	17059.5
63	15438.0
64	12141.5
65	8845.0
66	7399.0
67	5953.0
68	4744.5
69	3536.0
70	2978.0
71	2420.0
72	2093.0
73	1766.0
74	1336.5
75	684.0
76	461.0
77	326.5
78	192.0
79	175.5
80	159.0
81	118.0
82	77.0
83	43.5
84	10.0
85	9.5
86	9.0
87	6.5
88	4.0
89	5.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.98682202159809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20956088474097	26.345794378385218
2	9.671649093792615	6.961038291873306
3	3.6405678795196676	3.9303740501346307
4	2.040335892677916	2.9370081853631445
5	1.3915472595199494	2.5038681781493493
6	1.1498477449513815	2.4827619689700766
7	0.9381852940095824	2.363361503916246
8	0.8316593889404746	2.394302272991351
9	0.7431522704793779	2.4069319643419105
>10	6.286430565935827	37.85567449341989
>50	0.05964809942763839	1.4639440569357771
>100	0.02982404971381854	1.941648009398925
>500	0.0032535326960529323	0.9704337236925483
>1k	0.0027112772467107766	1.788072433178126
>5k	0.0016267663480264661	3.6547864892494943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	6688	1.2905491587695788	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	6370	1.2291863249644461	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	5671	1.0943038695248626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2731	0.5269871041566566	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	2659	0.513093632351721	TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	1442	0.27825536587107247	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT	1217	0.2348382664806485	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT	1114	0.21496288320414333	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC	976	0.18833372891135	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA	903	0.1742472922202347	No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	856	0.16517794256979057	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	811	0.1564945226917058	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT	769	0.14838999747216	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	658	0.12697089510621754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9296488617966188E-4	0.0	0.0	0.16421311813889228	0.0
2	1.9296488617966188E-4	0.0	0.0	1.0136445471017639	0.0
3	1.9296488617966188E-4	0.0	0.0	1.3517190276885316	0.0
4	1.9296488617966188E-4	0.0	0.0	2.0353936194230737	0.0
5	1.9296488617966188E-4	0.0	0.0	4.400757194213369	0.0
6	1.9296488617966188E-4	0.0	0.0	5.33625096241237	0.0
7	1.9296488617966188E-4	0.0	0.0	6.2671135733430585	0.0
8	1.9296488617966188E-4	0.0	0.0	7.548979312234553	0.0
9	1.9296488617966188E-4	0.0	0.0	7.970221658764754	0.0
10	1.9296488617966188E-4	0.0	0.0	9.995388139220307	0.0
11	1.9296488617966188E-4	0.0	0.0	11.27185086129877	0.0
12	1.9296488617966188E-4	0.0	0.0	13.527996310511377	0.0
13	1.9296488617966188E-4	0.0	0.0	14.066947237611172	0.0
14	1.9296488617966188E-4	0.0	0.0	14.312012643059342	0.0
15	1.9296488617966188E-4	0.0	0.0	14.816229890646799	0.0
16	1.9296488617966188E-4	0.0	0.0	15.567635157430402	0.0
17	1.9296488617966188E-4	0.0	0.0	16.40664648253957	0.0
18	1.9296488617966188E-4	0.0	0.0	17.284057819998495	0.0
19	1.9296488617966188E-4	0.0	0.0	18.032182683717043	0.0
20	1.9296488617966188E-4	0.0	0.0	18.58734266125593	0.0
21	3.8592977235932377E-4	0.0	0.0	19.218530803949605	0.0
22	3.8592977235932377E-4	0.0	0.0	19.949095863025804	0.0
23	3.8592977235932377E-4	0.0	0.0	20.639910155548996	0.0
24	3.8592977235932377E-4	0.0	0.0	21.1721073116325	0.0
25	3.8592977235932377E-4	0.0	0.0	21.695814012724103	0.0
26	3.8592977235932377E-4	0.0	0.0	22.17861215794562	0.0
27	3.8592977235932377E-4	0.0	0.0	22.697494736882728	0.0
28	5.788946585389856E-4	0.0	0.0	23.161768253030996	0.0
29	5.788946585389856E-4	0.0	0.0	23.651127204382618	0.0
30	5.788946585389856E-4	0.0	0.0	24.216900250661386	0.0
31	5.788946585389856E-4	0.0	0.0	24.714170762346377	0.0
32	5.788946585389856E-4	0.0	0.0	25.218002080161472	0.0
33	5.788946585389856E-4	0.0	0.0	25.718938924683876	0.0
34	5.788946585389856E-4	0.0	0.0	26.1969129477509	0.0
35	5.788946585389856E-4	0.0	0.0	26.69823572204566	0.0
36	5.788946585389856E-4	0.0	0.0	27.19569919861683	0.0
37	5.788946585389856E-4	0.0	0.0	27.692583780529457	0.0
38	5.788946585389856E-4	0.0	0.0	28.18657388914939	0.0
39	5.788946585389856E-4	0.0	0.0	28.681914751972585	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	30	2.1631859E-6	45.000004	16
CTTCGTC	30	2.1631859E-6	45.000004	36
CACCTCG	20	7.029696E-4	45.000004	16
TCGCTAT	20	7.029696E-4	45.000004	23
GCGTCTA	20	7.029696E-4	45.000004	24
CGACGGT	25	3.887831E-5	45.0	28
TTAGCCG	25	3.887831E-5	45.0	1
GCGAACA	35	1.21021E-7	45.0	33
CGGGGTA	55	1.8189894E-12	45.0	6
TACGGCT	765	0.0	41.47059	7
CGTAAGG	60	3.6379788E-12	41.250004	2
CCGATGA	670	0.0	40.97015	18
CGTTTTT	1200	0.0	40.6875	1
CGATGAA	675	0.0	40.666668	19
ACGGGCG	85	0.0	39.705883	5
CGGGAAT	125	0.0	39.600002	6
GATGAAT	725	0.0	39.41379	20
CCGATTA	40	3.453315E-7	39.375004	18
TAGCACG	40	3.453315E-7	39.375004	1
CTCGAAT	75	0.0	39.0	43
>>END_MODULE