##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935473.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554955
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166456739735654	31.0	31.0	33.0	28.0	34.0
2	31.22247749817553	31.0	31.0	34.0	28.0	34.0
3	30.970208395275293	31.0	31.0	34.0	27.0	34.0
4	34.736039859087676	35.0	35.0	37.0	32.0	37.0
5	34.81502824553342	35.0	35.0	37.0	33.0	37.0
6	35.01519222279284	36.0	35.0	37.0	32.0	37.0
7	35.34091593012046	37.0	35.0	37.0	33.0	37.0
8	35.1612419024966	37.0	35.0	37.0	32.0	37.0
9	37.034972204953554	39.0	37.0	39.0	33.0	39.0
10	36.48338874323143	38.0	35.0	39.0	32.0	39.0
11	36.765854889135156	39.0	37.0	39.0	33.0	39.0
12	36.8481372363525	39.0	37.0	39.0	33.0	39.0
13	36.90333991044319	39.0	37.0	39.0	33.0	39.0
14	37.87662242884558	40.0	37.0	41.0	33.0	41.0
15	38.084412249641865	40.0	37.0	41.0	33.0	41.0
16	38.04558387617014	40.0	37.0	41.0	33.0	41.0
17	37.96622068456001	40.0	37.0	41.0	33.0	41.0
18	37.85668027137336	39.0	37.0	41.0	33.0	41.0
19	37.634458649800436	39.0	37.0	40.0	33.0	41.0
20	37.60944761286951	39.0	35.0	41.0	33.0	41.0
21	37.53534610914399	39.0	35.0	41.0	33.0	41.0
22	37.634334315394945	39.0	35.0	41.0	33.0	41.0
23	37.70199385535764	39.0	35.0	41.0	33.0	41.0
24	37.67679361389662	39.0	35.0	41.0	33.0	41.0
25	37.27080033516231	39.0	35.0	41.0	33.0	41.0
26	37.34861385157355	39.0	35.0	41.0	33.0	41.0
27	37.41551116757215	39.0	35.0	41.0	33.0	41.0
28	37.29903866079232	39.0	35.0	41.0	33.0	41.0
29	37.23553981854385	39.0	35.0	41.0	33.0	41.0
30	36.89817192384968	39.0	35.0	41.0	32.0	41.0
31	36.78217693326486	39.0	35.0	41.0	31.0	41.0
32	36.64887963888964	39.0	35.0	41.0	31.0	41.0
33	36.453404330080815	39.0	35.0	41.0	30.0	41.0
34	36.218677190042435	39.0	35.0	41.0	30.0	41.0
35	36.14235208260129	39.0	35.0	41.0	30.0	41.0
36	35.95247542593544	39.0	35.0	41.0	29.0	41.0
37	35.92803560649062	39.0	35.0	41.0	29.0	41.0
38	35.76485120415169	39.0	35.0	41.0	27.0	41.0
39	35.66571704012037	39.0	35.0	41.0	27.0	41.0
40	35.574713265039506	39.0	35.0	41.0	26.0	41.0
41	35.37399248587723	38.0	35.0	40.0	25.0	41.0
42	35.39235793893199	38.0	35.0	40.0	26.0	41.0
43	35.360638249948195	38.0	35.0	40.0	26.0	41.0
44	35.33494968060474	38.0	35.0	40.0	26.0	41.0
45	35.2969087583678	38.0	35.0	40.0	26.0	41.0
46	35.19408961086935	38.0	35.0	40.0	25.0	41.0
47	35.16224198358425	38.0	35.0	40.0	25.0	41.0
48	35.0618933066645	38.0	34.0	40.0	25.0	41.0
49	35.03104215657125	38.0	34.0	40.0	25.0	41.0
50	34.91162526691353	38.0	34.0	40.0	24.0	41.0
51	33.69972700489229	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	21.0
9	26.0
10	34.0
11	27.0
12	35.0
13	23.0
14	20.0
15	39.0
16	67.0
17	156.0
18	280.0
19	436.0
20	783.0
21	1189.0
22	1865.0
23	2872.0
24	4268.0
25	6614.0
26	8737.0
27	9413.0
28	9459.0
29	9355.0
30	10405.0
31	12356.0
32	15996.0
33	22570.0
34	38119.0
35	42227.0
36	45097.0
37	68210.0
38	117557.0
39	126678.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.97279058662414	19.476173743817064	21.28875314214666	19.262282527412133
2	39.93765260246327	22.106657296537556	22.631384526673333	15.324305574325848
3	30.179564108801614	21.913128091466877	32.17215810290924	15.735149696822265
4	27.076249425629108	23.19413285761909	31.904208449333733	17.82540926741808
5	24.006270778711787	27.176617923975815	30.288942346676755	18.52816895063564
6	23.174311430656537	31.470839978016237	30.87691794830211	14.477930643025111
7	71.85861916731987	5.510897279959636	17.20031353893559	5.430170013784901
8	72.21108017767206	5.464947608364642	16.751628510419764	5.572343703543531
9	65.69613752466417	6.949572487859376	19.160112081159735	8.194177906316728
10	36.24167725311061	22.196394302240723	26.08121379210927	15.480714652539396
11	27.813966898216975	22.724184843816165	30.781594904091325	18.68025335387554
12	24.701282085934896	19.906659098485463	34.99382832842303	20.398230487156617
13	23.883918515915706	20.26092205674334	37.0309304358011	18.824228991539854
14	19.52104224666865	23.932210719788092	36.39159931886369	20.15514771467957
15	18.536277716211224	23.70876917948302	38.392302078546905	19.362651025758844
16	22.10521573821301	23.864998062906	34.61145498283645	19.418331216044542
17	22.030795289708173	23.70462469929994	32.8594210341379	21.40515897685398
18	22.495157265003467	23.57452406050941	34.06023911848708	19.870079556000036
19	21.77672063500644	25.808939463560108	31.87033182870683	20.544008072726616
20	21.807353749403106	26.274923191970522	32.004757142471014	19.912965916155365
21	22.13729041093422	25.6006342856628	34.21772936544404	18.044345937958933
22	20.817003180438054	23.116649097674586	34.16637385013199	21.899973871755368
23	19.937832797253833	24.382517501419034	34.144750475263756	21.534899226063374
24	20.93106648286798	23.580830878179313	33.48433656782983	22.003766071122886
25	21.939256336099323	25.331783658134444	31.2351451919525	21.49381481381373
26	20.203259723761384	24.594066185546577	32.030344802731754	23.172329287960284
27	21.359029110468416	23.442261084232054	32.87689992882306	22.32180987647647
28	20.05045454135921	23.989873052770044	32.52335774972745	23.436314656143292
29	22.608679983061688	23.117009487255725	31.96042922399113	22.313881305691453
30	23.328558171383264	22.22504527394113	33.414240794298635	21.032155760376966
31	22.93393158003802	22.251353713364146	32.466776585488915	22.347938121108918
32	25.365660278761343	22.425782270634556	30.935301060446342	21.273256390157762
33	23.659756196448363	22.744727049940984	31.914299357605568	21.68121739600508
34	21.96934886612428	22.865097170040812	33.796253750304075	21.369300213530824
35	23.348019208764676	23.284590642484527	32.81635447919201	20.551035669558793
36	23.684803272337398	25.19303366939662	30.686452054671097	20.435711003594886
37	22.32595435665955	25.737942716076077	31.50345523510915	20.43264769215522
38	22.410285518645658	25.148345361335604	30.86052022236037	21.58084889765837
39	22.479119928642863	24.168085700642393	30.861781585894356	22.49101278482039
40	23.830941247488536	22.84095106810462	32.89185609644025	20.43625158796659
41	21.438314818318602	24.289897379066772	31.384346478543307	22.887441324071318
42	21.89835211864025	24.680379490228937	31.560216594138264	21.861051796992548
43	21.749511221630584	23.897973709580057	31.753745799208943	22.598769269580416
44	22.552999792775992	23.209089025236278	30.74952023137011	23.488390950617617
45	21.734374859222818	22.53281797623231	30.604283230171813	25.12852393437306
46	22.643097188060292	23.385499725202944	30.46805596850195	23.503347118234814
47	20.672306763611463	23.530736726401237	33.28540151904208	22.51155499094521
48	20.750691497508807	23.32441369120019	32.65616131037652	23.268733500914486
49	21.789514465136815	22.05440080727266	32.697966501788436	23.458118225802092
50	20.226324656954166	22.527051742934113	33.84616770729158	23.400455892820137
51	20.285608743051238	22.105395933003578	32.25432692740853	25.354668396536656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	235.0
1	481.0
2	727.0
3	8489.5
4	16252.0
5	10243.5
6	4235.0
7	3999.5
8	3764.0
9	3802.5
10	3841.0
11	3772.5
12	3704.0
13	3508.0
14	3312.0
15	3120.0
16	2928.0
17	2795.0
18	2662.0
19	2545.0
20	2428.0
21	2417.0
22	2406.0
23	2584.5
24	2763.0
25	3116.5
26	3823.0
27	4176.0
28	4888.5
29	5601.0
30	6473.0
31	7345.0
32	8647.5
33	9950.0
34	11164.5
35	12379.0
36	13571.0
37	14763.0
38	16266.5
39	17770.0
40	19138.5
41	20507.0
42	22724.0
43	24941.0
44	27837.0
45	30733.0
46	41055.5
47	51378.0
48	53548.5
49	55719.0
50	55259.0
51	54799.0
52	47815.5
53	40832.0
54	36585.5
55	32339.0
56	29817.0
57	27295.0
58	25185.0
59	23075.0
60	21174.0
61	19273.0
62	17508.5
63	15744.0
64	13756.5
65	11769.0
66	9658.0
67	7547.0
68	6473.5
69	5400.0
70	4579.0
71	3758.0
72	3050.0
73	2342.0
74	1879.5
75	1055.0
76	693.0
77	508.0
78	323.0
79	247.0
80	171.0
81	137.0
82	103.0
83	76.5
84	50.0
85	35.5
86	21.0
87	13.5
88	6.0
89	5.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	554955.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.161841887459964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32821274774449	31.338143536478892
2	9.008769803014252	7.5965265607042145
3	3.799500083093213	4.805817652643011
4	2.3448206709257904	3.9544783352808386
5	1.6901741636828542	3.5630427925733192
6	1.399236569191524	3.5396634596042977
7	1.155732893203191	3.4109479255158703
8	0.991663795132316	3.3448297708709758
9	0.8277610036349371	3.1409935700275255
>10	4.3885542570395275	26.840962341505524
>50	0.037532494483341644	1.0716932373309556
>100	0.021138991136507513	1.6608334940157354
>500	0.0021570399118885216	0.6382504359326706
>1k	0.004314079823777043	3.0499239982100375
>5k	0.0	0.0
>10k+	4.3140798237770433E-4	2.0438928893061896
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11237	2.0248488616194105	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT	2808	0.5059869719166419	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	2410	0.43426944527033723	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	2174	0.3917434746961465	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	2058	0.37084087899018836	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT	1602	0.28867205449090466	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1276	0.22992855276553953	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1244	0.22416231946734422	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	1120	0.20181816543683723	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	1047	0.18866394572532907	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC	1029	0.18542043949509418	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	961	0.17316719373642908	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCC	703	0.12667693776972908	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC	687	0.1237938211206314	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	617	0.11118018578082907	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6038958113720934E-4	0.0	0.0	0.18055518014974187	0.0
2	3.6038958113720934E-4	0.0	0.0	0.7463668225351605	0.0
3	3.6038958113720934E-4	0.0	0.0	1.3597498896306908	0.0
4	3.6038958113720934E-4	0.0	0.0	1.9989008117775315	0.0
5	3.6038958113720934E-4	0.0	0.0	3.815804885080772	0.0
6	3.6038958113720934E-4	0.0	0.0	5.574686235820923	0.0
7	5.40584371705814E-4	0.0	0.0	6.861817624852465	0.0
8	5.40584371705814E-4	0.0	0.0	8.403744447748016	0.0
9	5.40584371705814E-4	0.0	0.0	9.242010613473164	0.0
10	5.40584371705814E-4	0.0	0.0	10.643205304934634	0.0
11	5.40584371705814E-4	0.0	0.0	11.963852925011938	0.0
12	5.40584371705814E-4	0.0	0.0	13.17998756655945	0.0
13	5.40584371705814E-4	0.0	0.0	13.7611157661432	0.0
14	5.40584371705814E-4	0.0	0.0	14.090692038093179	0.0
15	5.40584371705814E-4	0.0	0.0	14.482075123208189	0.0
16	5.40584371705814E-4	0.0	0.0	15.067888387346722	0.0
17	5.40584371705814E-4	0.0	0.0	15.696588011640584	0.0
18	5.40584371705814E-4	0.0	0.0	16.387274643890045	0.0
19	5.40584371705814E-4	0.0	0.0	16.837761620311557	0.0
20	5.40584371705814E-4	0.0	0.0	17.28896937589534	0.0
21	5.40584371705814E-4	0.0	0.0	17.82018361849159	0.0
22	5.40584371705814E-4	0.0	0.0	18.38076961195052	0.0
23	5.40584371705814E-4	0.0	0.0	18.926579632582822	0.0
24	5.40584371705814E-4	0.0	0.0	19.379228946491157	0.0
25	5.40584371705814E-4	0.0	0.0	19.81151624906524	0.0
26	5.40584371705814E-4	0.0	0.0	20.199836022740584	0.0
27	5.40584371705814E-4	0.0	0.0	20.63122235136182	0.0
28	5.40584371705814E-4	0.0	0.0	21.03053400726185	0.0
29	5.40584371705814E-4	0.0	0.0	21.46714598480958	0.0
30	5.40584371705814E-4	0.0	0.0	22.08143002585795	0.0
31	5.40584371705814E-4	0.0	0.0	22.55930661044589	0.0
32	7.207791622744187E-4	0.0	0.0	23.071240010451298	0.0
33	7.207791622744187E-4	0.0	0.0	23.51361822129722	0.0
34	7.207791622744187E-4	0.0	0.0	23.972754547666028	0.0
35	7.207791622744187E-4	0.0	0.0	24.4659476894523	0.0
36	7.207791622744187E-4	0.0	0.0	24.922020704381435	0.0
37	9.009739528430233E-4	0.0	0.0	25.401519042084495	0.0
38	9.009739528430233E-4	0.0	0.0	25.924444324314585	0.0
39	9.009739528430233E-4	0.0	0.0	26.651350109468336	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	20	7.0301286E-4	45.000004	1
TACTAGT	20	7.0301286E-4	45.000004	2
CCGCAAT	20	7.0301286E-4	45.000004	41
CGATCGA	25	3.8881888E-5	45.0	41
CGTTTTT	4555	0.0	43.863888	1
CCATACG	60	3.6379788E-12	41.249996	2
CGTTCTG	230	0.0	41.08696	1
CGGTCTA	50	1.0786607E-9	40.5	31
CGTTTCT	325	0.0	39.46154	1
TATGGGT	110	0.0	38.86364	4
ACGGGTA	70	0.0	38.571426	5
AACGGGA	175	0.0	38.571426	4
CGATAAG	35	6.2424515E-6	38.571426	10
GTTTTTT	5360	0.0	37.94776	2
AGCACGG	90	0.0	37.5	2
TCGCAAG	30	1.1391613E-4	37.499996	1
CCGATCG	30	1.1391613E-4	37.499996	40
ACGGGTC	30	1.1391613E-4	37.499996	5
GGGCGAT	645	0.0	36.97674	7
TCACGAC	55	2.743036E-9	36.818184	25
>>END_MODULE