FastQCFastQC Report
Sat 14 Jan 2017
SRR2935472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489786
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159313.252645032728579No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC33240.6786637429407946No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT27120.5537112126520561No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC18250.37261171205383575No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG18040.3683241252302026No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT15930.3252440861927454No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC14150.2889016835924261No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG13950.2848182675699183No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC12050.2460258153560943No Hit
CGTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9890.20192492231301015No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9880.2017207515118848No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC8320.16987010653632403No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC7540.15394478404854364No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC6970.14230704838439645No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG6830.139448657168641No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT6380.13026097111799848No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT5780.11801072305047511Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGT5720.11678569824372276No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG5290.10800635379533102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG351.21001E-745.00000420
ACGCATG207.029316E-445.0000041
ATCGTTG302.1629403E-645.00000423
ACGATAG207.029316E-445.0000041
GTCGATG253.8875165E-545.041
ACTTGCG253.8875165E-545.01
CGTTTTT68150.044.2406461
CGTTTTC1850.041.3513531
TACGCGG451.9244908E-840.02
CGTTCTG1700.039.7058831
CGTTTCT3250.039.461541
TAGGGCG1200.039.3750045
AGTTACG403.4528057E-739.3750041
GTTTTTT76800.039.3457072
AGCGACT1550.039.1935519
TACGGGT750.039.0000044
CGTTGAT356.240947E-638.5714325
ATCACTC356.240947E-638.5714325
TCGACGG356.240947E-638.571432
AATCGTT356.240947E-638.5714322
Produced by FastQC (version 0.11.5)