##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935471.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173813
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.042706817096533	31.0	30.0	33.0	28.0	34.0
2	31.032045934423778	31.0	31.0	34.0	28.0	34.0
3	30.844844746940677	31.0	30.0	34.0	27.0	34.0
4	34.799318808144385	35.0	35.0	37.0	32.0	37.0
5	34.71467036412697	35.0	35.0	37.0	32.0	37.0
6	34.88522147365272	35.0	35.0	37.0	32.0	37.0
7	35.31119076248612	36.0	35.0	37.0	33.0	37.0
8	34.95111988171195	37.0	35.0	37.0	32.0	37.0
9	36.6638111073395	39.0	37.0	39.0	32.0	39.0
10	36.15334871384764	37.0	35.0	39.0	32.0	39.0
11	36.44845322271637	38.0	35.0	39.0	32.0	39.0
12	36.49124058614718	38.0	35.0	39.0	32.0	39.0
13	36.364943933998035	39.0	35.0	39.0	32.0	39.0
14	37.332840466478345	39.0	36.0	40.0	32.0	41.0
15	37.501533257006095	39.0	36.0	41.0	32.0	41.0
16	37.629417822602456	39.0	36.0	41.0	33.0	41.0
17	37.4070984333738	39.0	36.0	40.0	32.0	41.0
18	37.49307589190682	39.0	36.0	40.0	33.0	41.0
19	37.38865907613354	39.0	36.0	40.0	32.0	41.0
20	37.208062688061304	39.0	35.0	40.0	32.0	41.0
21	37.40719623963685	39.0	35.0	40.0	33.0	41.0
22	37.50480113685398	39.0	35.0	41.0	33.0	41.0
23	37.466133143090566	39.0	35.0	41.0	33.0	41.0
24	37.45273943836192	39.0	35.0	41.0	33.0	41.0
25	37.2022173255165	39.0	35.0	40.0	32.0	41.0
26	37.16863525743184	39.0	35.0	41.0	32.0	41.0
27	37.122988499134124	39.0	35.0	41.0	32.0	41.0
28	36.99449983603068	39.0	35.0	41.0	31.0	41.0
29	36.97355778911819	39.0	35.0	41.0	31.0	41.0
30	36.76697370162186	39.0	35.0	40.0	31.0	41.0
31	36.77601790429945	39.0	35.0	40.0	31.0	41.0
32	36.624763395143056	39.0	35.0	40.0	31.0	41.0
33	36.60626650480689	39.0	35.0	41.0	30.0	41.0
34	36.36769401598269	39.0	35.0	41.0	30.0	41.0
35	36.44390235482962	39.0	35.0	41.0	30.0	41.0
36	36.1965675754978	39.0	35.0	41.0	30.0	41.0
37	36.140990604845435	39.0	35.0	41.0	30.0	41.0
38	36.05976538003487	39.0	35.0	40.0	29.0	41.0
39	35.9419203396754	39.0	35.0	40.0	29.0	41.0
40	35.77859538699637	38.0	35.0	40.0	28.0	41.0
41	35.50488168318825	38.0	35.0	40.0	26.0	41.0
42	35.68750898954624	38.0	35.0	40.0	28.0	41.0
43	35.562132866931705	38.0	35.0	40.0	28.0	41.0
44	35.65740192045474	38.0	35.0	40.0	28.0	41.0
45	35.63274898885584	38.0	35.0	40.0	28.0	41.0
46	35.4989385143804	38.0	35.0	40.0	28.0	41.0
47	35.24662712225208	38.0	34.0	40.0	27.0	41.0
48	35.26540592475822	38.0	34.0	40.0	27.0	41.0
49	35.23480982435146	37.0	34.0	40.0	28.0	41.0
50	35.01665583126694	37.0	34.0	40.0	27.0	41.0
51	33.79529149143045	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	8.0
11	10.0
12	3.0
13	8.0
14	4.0
15	4.0
16	10.0
17	43.0
18	68.0
19	143.0
20	378.0
21	649.0
22	943.0
23	1252.0
24	1379.0
25	1550.0
26	1760.0
27	2001.0
28	2274.0
29	2634.0
30	3400.0
31	4406.0
32	5662.0
33	7935.0
34	13690.0
35	15085.0
36	15067.0
37	22325.0
38	36192.0
39	34914.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.316293948093644	19.03137279720159	21.420146939526962	11.232186315177806
2	29.977619625689677	31.237594426193667	22.328019193040795	16.456766755075858
3	28.294201239262883	31.388906468445978	24.88651596831077	15.430376323980369
4	27.31556327777554	22.04380569922848	33.059092242812675	17.5815387801833
5	30.18013612330493	26.967488047499323	23.52298159516262	19.329394234033128
6	22.270486097127375	38.43671071784044	24.56260463831819	14.730198546713996
7	71.35312088278782	5.553094417563704	17.960681882252764	5.133102817395708
8	70.32557978977407	11.904748206405735	11.035423127153896	6.734248876666301
9	65.36852824587343	6.438528763671301	12.629665214914878	15.56327777554038
10	42.59347689758533	21.053085787599315	20.45646758297711	15.89696973183824
11	32.33244924142613	21.904575607117994	25.277741020522054	20.48523413093382
12	25.998055381358125	18.58376531099515	30.87456059097996	24.543618716666764
13	25.08615581113035	22.781955319797714	34.45887246638629	17.673016402685647
14	19.5641292653599	31.189266625626395	27.75051348288103	21.496090626132684
15	15.817574059477716	23.012663034410544	40.126457744817706	21.043305161294036
16	18.7828298228556	23.245672072859914	30.578265147025828	27.39323295725866
17	18.535437510427872	25.52513333294978	30.72670053448246	25.212728622139885
18	19.782179698871776	24.054587401402657	31.354961941857052	24.808270957868515
19	20.98347074154407	25.77310097633664	26.990501285864692	26.252926996254594
20	23.93721988573927	25.437683027161373	31.594299620856898	19.030797466242454
21	21.669840575791223	29.3579881827021	29.847594828925338	19.124576412581337
22	18.39390609448085	23.390655474561743	31.271538952782592	26.94389947817482
23	20.240718473301765	27.624516002830628	29.037528838464326	23.097236685403278
24	22.54952161230748	24.93541909983718	27.974317225984247	24.54074206187109
25	18.59066928250476	31.21860850454224	26.920886239809448	23.26983597314355
26	19.660209535535316	24.9037758970848	30.240545874014025	25.195468693365857
27	24.394607998250994	27.13260803277085	26.39215708836508	22.08062688061307
28	17.182259094544136	26.66083664628077	31.685201912400107	24.471702346774983
29	24.31923964260441	23.0891820519754	29.22911404785603	23.362464257564163
30	23.911905323537365	27.06471897959301	27.98122119749386	21.042154499375766
31	23.594322634095263	25.72477317576936	22.609931363016575	28.0709728271188
32	26.85069586279507	28.6020033023997	22.768722707737627	21.778578127067597
33	25.760443695235686	23.208275560516185	24.60287780545759	26.42840293879054
34	23.690978235229814	26.510675265946738	25.211002629262484	24.587343869560964
35	24.853146772680983	23.34060168111706	24.665588880003224	27.14066266619873
36	26.55842773555488	24.082778618400237	26.595248916939468	22.763544729105416
37	23.057538849223018	27.19877109307129	28.223435531289375	21.52025452641632
38	21.652005316058062	25.87378389418513	23.04488156812206	29.42932922163475
39	23.19791960325177	28.771725935344307	26.44738886044197	21.582965600961952
40	24.513701506791783	22.623739306035798	27.240770253088087	25.621788934084332
41	20.149816181758556	25.74260843550252	26.68902786327835	27.418547519460567
42	21.669840575791223	21.883288361630026	29.74748724203598	26.699383820542767
43	25.00273282205589	22.55527492189882	27.09751284426366	25.344479411781627
44	22.711764942783336	23.648403744253883	28.073274150955335	25.56655716200745
45	20.70385989540483	23.224384827371946	26.17525731677147	29.89649796045175
46	27.34663114956879	25.739731780706855	25.422724422223887	21.490912647500473
47	19.425474504208545	23.848043587073462	33.45146795694223	23.275013951775758
48	21.38562708197891	24.875009349128085	27.1234027374247	26.6159608314683
49	21.187137901077595	20.375921248698315	34.01701828977119	24.4199225604529
50	22.654231846869912	21.813673315574782	29.52253283701449	26.00956200054081
51	20.39720849418628	22.153693912423122	26.51815456841548	30.930943024975114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	161.5
2	215.0
3	611.5
4	1008.0
5	701.5
6	395.0
7	377.0
8	359.0
9	369.5
10	380.0
11	374.0
12	368.0
13	361.0
14	354.0
15	355.0
16	356.0
17	338.5
18	321.0
19	352.0
20	383.0
21	374.0
22	365.0
23	489.5
24	614.0
25	660.5
26	948.5
27	1190.0
28	1177.5
29	1165.0
30	1533.0
31	1901.0
32	2357.0
33	2813.0
34	2815.0
35	2817.0
36	3244.0
37	3671.0
38	4158.0
39	4645.0
40	5556.0
41	6467.0
42	7554.0
43	8641.0
44	9087.5
45	9534.0
46	10868.0
47	12202.0
48	20182.0
49	28162.0
50	24145.0
51	20128.0
52	19398.0
53	18668.0
54	15622.5
55	12577.0
56	10802.0
57	9027.0
58	7927.5
59	6828.0
60	6243.5
61	5659.0
62	4974.5
63	4290.0
64	3483.5
65	2677.0
66	2279.5
67	1882.0
68	1468.0
69	1054.0
70	893.0
71	732.0
72	592.0
73	452.0
74	406.5
75	282.0
76	203.0
77	137.0
78	71.0
79	53.5
80	36.0
81	24.5
82	13.0
83	8.5
84	4.0
85	3.5
86	3.0
87	4.0
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	173813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.08173151605461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41508664319923	28.71937081806309
2	9.681074466317172	7.373441572264445
3	3.4249369249595865	3.9128258530719795
4	1.8446616609508846	2.809916404411638
5	1.2116450877007447	2.3070771461283104
6	0.8747412790258493	1.9986997520323564
7	0.7372603526159146	1.9653305564025707
8	0.6632321614721035	2.020562328479458
9	0.5907147497393905	2.0245896451933976
>10	5.473553806408726	35.29712967384488
>50	0.046834161744043756	1.2801113840736884
>100	0.021150911755374598	1.5729548422730177
>500	0.009064676466589114	2.0671641361693314
>1k	0.006043117644392742	6.650825887591837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	3436	1.9768371755852554	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	3206	1.8445110549843795	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	3172	1.8249498023738158	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1746	1.0045278546483865	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCT	699	0.4021563404348351	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCAGACTT	694	0.3992796856391639	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTA	575	0.33081530150218913	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	572	0.3290893086247864	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTC	541	0.3112540488916249	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	512	0.2945694510767319	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	470	0.2704055507930937	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	469	0.2698302198339595	No Hit
TCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	274	0.1576406828027823	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATG	174	0.10010758688935809	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28593948668971825	0.0
2	0.0	0.0	0.0	1.9417419870780666	0.0
3	0.0	0.0	0.0	2.3772675231426876	0.0
4	0.0	0.0	0.0	3.239113299925782	0.0
5	0.0	0.0	0.0	6.6364426136134815	0.0
6	0.0	0.0	0.0	7.451686582706702	0.0
7	0.0	0.0	0.0	8.672538877989563	0.0
8	0.0	0.0	0.0	10.328916709337046	0.0
9	0.0	0.0	0.0	10.773072209788682	0.0
10	0.0	0.0	0.0	13.717040727678597	0.0
11	0.0	0.0	0.0	15.223832509651176	0.0
12	0.0	0.0	0.0	18.557300086874974	0.0
13	0.0	0.0	0.0	19.231012640021174	0.0
14	0.0	0.0	0.0	19.539390034117126	0.0
15	0.0	0.0	0.0	20.260855056871467	0.0
16	0.0	0.0	0.0	21.156070029284347	0.0
17	0.0	0.0	0.0	22.27278742096391	0.0
18	0.0	0.00862996438701363	0.0	23.388354150725206	0.0
19	0.0	0.00862996438701363	0.0	24.500468894731693	0.0
20	0.0	0.00862996438701363	0.0	25.16842813828655	0.0
21	0.0	0.00862996438701363	0.0	25.85594863445197	0.0
22	0.0	0.00862996438701363	0.0	26.641850724629343	0.0
23	0.0	0.00862996438701363	0.0	27.40358891452308	0.0
24	0.0	0.00862996438701363	0.0	28.00193311202269	0.0
25	0.0	0.00862996438701363	0.0	28.49671773687814	0.0
26	0.0	0.00862996438701363	0.0	28.9782697496735	0.0
27	0.0	0.00862996438701363	0.0	29.474205036447216	0.0
28	0.0	0.00862996438701363	0.0	29.96553767554786	0.0
29	0.0	0.00862996438701363	0.0	30.47182891958599	0.0
30	0.0	0.00862996438701363	0.0	31.048885871597637	0.0
31	0.0	0.00862996438701363	0.0	31.562656418104513	0.0
32	0.0	0.00862996438701363	0.0	32.0413317761042	0.0
33	0.0	0.00862996438701363	0.0	32.543020372469265	0.0
34	0.0	0.00862996438701363	0.0	33.064270221444886	0.0
35	0.0	0.00862996438701363	0.0	33.57171212740129	0.0
36	0.0	0.00862996438701363	0.0	34.05383947115578	0.0
37	0.0	0.00862996438701363	0.0	34.52503552668673	0.0
38	0.0	0.00862996438701363	0.0	35.03247743264313	0.0
39	0.0	0.00862996438701363	0.0	35.59802776547209	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGTC	25	3.8771068E-5	45.000004	6
AAGCATA	25	3.8771068E-5	45.000004	25
GTCCGGG	25	3.8771068E-5	45.000004	3
CGAGCAC	50	2.1827873E-11	45.000004	34
GAACCGT	25	3.8771068E-5	45.000004	36
CGTCCTA	25	3.8771068E-5	45.000004	40
TCACGGG	40	6.7575456E-9	45.0	3
TCACGAC	20	7.0167525E-4	45.0	25
ACAACGG	20	7.0167525E-4	45.0	2
CTCACGA	20	7.0167525E-4	45.0	24
TCGGGGT	20	7.0167525E-4	45.0	5
AACGGGC	20	7.0167525E-4	45.0	4
CCATCGA	40	6.7575456E-9	45.0	41
CGAATGC	40	6.7575456E-9	45.0	45
CCCATCG	45	3.8016879E-10	45.0	40
ATCGAGG	20	7.0167525E-4	45.0	2
ATCGAAT	40	6.7575456E-9	45.0	43
CCCTCGA	30	2.154844E-6	44.999996	41
GGATCCT	30	2.154844E-6	44.999996	8
GCATTCT	30	2.154844E-6	44.999996	9
>>END_MODULE