SRR2935470.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935470.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286874
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	4479	1.561312632026604	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC	3347	1.1667143066293912	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG	3252	1.1335987227842188	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2288	0.7975626930289952	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1633	0.5692394570438589	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT	1035	0.3607855713658261	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	895	0.31198365833083513	Illumina PCR Primer Index 8 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT	735	0.2562100434337026	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGGAAGCT	658	0.22936899126445756	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC	609	0.2122883217022107	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTA	558	0.19451048195374973	No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	543	0.18928170555714355	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	512	0.17847556767082412	Illumina PCR Primer Index 8 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	484	0.16871518506382593	Illumina PCR Primer Index 8 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	80	0.0	45.000004	31
CGACGGT	35	1.2076634E-7	45.000004	28
CGAATAT	20	7.024425E-4	45.000004	14
GTACGAG	20	7.024425E-4	45.000004	1
CACAACG	45	3.8198777E-10	45.0	12
TACACGG	25	3.8834634E-5	45.0	2
GGCGATA	110	0.0	42.954548	8
CGTTTTT	1135	0.0	42.81938	1
CCGATGA	630	0.0	42.5	18
CGATGAA	640	0.0	42.187504	19
CGGAAGG	55	6.002665E-11	40.909092	2
GGGACCG	105	0.0	40.714287	7
ACAACGA	50	1.0750227E-9	40.5	13
CGGGTAT	50	1.0750227E-9	40.5	6
CGAACAC	50	1.0750227E-9	40.5	34
CGCTCGA	50	1.0750227E-9	40.5	41
ACCGATG	675	0.0	40.333332	17
GATGAAT	680	0.0	40.036762	20
GTGCAAG	45	1.9195795E-8	40.0	1
CTCGAAT	85	0.0	39.705883	43