##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935470.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286874
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0904369165557	31.0	30.0	33.0	28.0	34.0
2	31.10599078341014	31.0	31.0	34.0	28.0	34.0
3	30.921062905665902	31.0	30.0	34.0	27.0	34.0
4	34.75871637025314	35.0	35.0	37.0	32.0	37.0
5	34.745247042255485	35.0	35.0	37.0	32.0	37.0
6	34.92457315755349	36.0	35.0	37.0	32.0	37.0
7	35.29207247781256	37.0	35.0	37.0	33.0	37.0
8	34.93293571393713	37.0	35.0	37.0	32.0	37.0
9	36.70942295223687	39.0	37.0	39.0	32.0	39.0
10	36.17132260156027	37.0	35.0	39.0	32.0	39.0
11	36.551583622077985	38.0	35.0	39.0	32.0	39.0
12	36.68206599412983	39.0	35.0	39.0	32.0	39.0
13	36.67283546086435	39.0	35.0	39.0	32.0	39.0
14	37.75008888919874	39.0	37.0	41.0	33.0	41.0
15	37.88035862434379	40.0	37.0	41.0	33.0	41.0
16	37.92170778808815	39.0	37.0	41.0	33.0	41.0
17	37.72576810725266	39.0	36.0	41.0	33.0	41.0
18	37.720961118818714	39.0	36.0	41.0	33.0	41.0
19	37.58659550882966	39.0	36.0	40.0	33.0	41.0
20	37.414331727518004	39.0	35.0	41.0	32.0	41.0
21	37.51123141169991	39.0	36.0	41.0	33.0	41.0
22	37.62277864149417	39.0	36.0	41.0	33.0	41.0
23	37.591625591723194	39.0	36.0	41.0	33.0	41.0
24	37.649337339738004	40.0	36.0	41.0	33.0	41.0
25	37.32160112104966	39.0	35.0	41.0	32.0	41.0
26	37.28506243159018	39.0	35.0	41.0	32.0	41.0
27	37.24614290594477	39.0	35.0	41.0	32.0	41.0
28	37.11902438004141	39.0	35.0	41.0	32.0	41.0
29	37.06958455628604	39.0	35.0	41.0	31.0	41.0
30	36.830737536339996	39.0	35.0	41.0	31.0	41.0
31	36.82214839964584	39.0	35.0	41.0	31.0	41.0
32	36.709924914770944	39.0	35.0	41.0	31.0	41.0
33	36.54069382376932	39.0	35.0	41.0	30.0	41.0
34	36.312196295237634	39.0	35.0	41.0	30.0	41.0
35	36.35224872243563	39.0	35.0	41.0	30.0	41.0
36	36.18188124403048	39.0	35.0	41.0	29.0	41.0
37	36.14409113408674	39.0	35.0	41.0	29.0	41.0
38	35.985523261083266	39.0	35.0	41.0	28.0	41.0
39	35.86163263314208	39.0	35.0	41.0	27.0	41.0
40	35.71646785696856	39.0	35.0	41.0	26.0	41.0
41	35.579048641563894	39.0	35.0	40.0	26.0	41.0
42	35.668837189846414	39.0	35.0	41.0	26.0	41.0
43	35.65565021577417	39.0	35.0	40.0	27.0	41.0
44	35.71238941137921	39.0	35.0	40.0	27.0	41.0
45	35.76498044437628	39.0	35.0	40.0	27.0	41.0
46	35.61618341153259	39.0	35.0	40.0	27.0	41.0
47	35.438363183836806	38.0	35.0	40.0	26.0	41.0
48	35.463653032341725	38.0	35.0	40.0	26.0	41.0
49	35.44768434922649	38.0	35.0	40.0	26.0	41.0
50	35.29924984487963	38.0	35.0	40.0	26.0	41.0
51	34.11063393685033	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	14.0
11	14.0
12	11.0
13	7.0
14	17.0
15	14.0
16	29.0
17	59.0
18	151.0
19	363.0
20	712.0
21	1192.0
22	1707.0
23	2112.0
24	2599.0
25	3145.0
26	3594.0
27	3849.0
28	3939.0
29	4268.0
30	4909.0
31	6288.0
32	8423.0
33	11338.0
34	18698.0
35	21515.0
36	23665.0
37	34782.0
38	60988.0
39	68450.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.95371487133724	19.110480559409357	21.135062780175268	12.800741789078133
2	33.37249105879236	29.07408827568898	21.892886772590057	15.660533892928601
3	29.902675042004507	28.91548205832526	26.38684579292651	14.794997106743727
4	26.270418371828747	22.775155643244073	33.23096551099089	17.723460473936292
5	28.720274406185293	27.348243479715833	24.766273695071703	19.16520841902717
6	22.276330375007845	37.398997469272224	26.26030940412864	14.064362751591291
7	71.4860879689341	5.947558858592971	17.730083590705327	4.836269581767605
8	71.19362507581725	10.939994562072547	12.325620307173184	5.54076005493701
9	65.77417263328151	7.167258099374639	13.711594637366927	13.346974629976923
10	38.26697435110885	23.00452463450853	23.02962276121224	15.698878253170381
11	31.15235260079338	24.543876405669387	25.31459804652914	18.989172947008093
12	25.08662339563711	20.573143610086657	30.58102163319088	23.759211361085356
13	25.989110201691336	22.32687521350837	33.58164211465661	18.10237247014369
14	19.462900088540614	30.420323905268514	29.428947900471986	20.687828105718886
15	17.81444118323724	25.203050816734873	35.938774514246674	21.043733485781214
16	20.003904153042797	25.32366125894992	30.626337695294797	24.04609689271248
17	20.35946094801202	26.656999240084495	30.0107364208677	22.972803391035786
18	20.93567210691802	25.172375328541452	30.121935065568856	23.770017498971676
19	21.124256642288948	27.211598123217858	27.71007480636098	23.954070428132212
20	24.420128697616374	25.670852011684573	30.789475518868908	19.11954377183014
21	22.141079358882294	29.471823866924158	30.172131318976277	18.21496545521727
22	20.03004803502583	24.2440932255973	31.71740903671995	24.008449702656915
23	21.3386364745498	27.40436567970607	29.72141079358882	21.535587052155304
24	21.805740499313288	25.519217496182993	29.272781778760017	23.402260225743706
25	20.26499438778	30.6165773126878	26.936215899663267	22.18221239986893
26	19.274664138262793	26.63782705996361	30.874878866680145	23.212629935093457
27	24.134637506361678	26.679308686043353	27.484540251120706	21.70151355647427
28	20.174710848665267	27.807330047337857	30.589736260518556	21.428222843478324
29	22.344304468163724	26.218130607862683	29.58894845820813	21.84861646576546
30	23.346486610846572	28.73247488444404	27.736567273437117	20.184471231272266
31	25.19607911487273	26.578219009042297	24.85864874474508	23.36705313133989
32	28.136742960324046	28.22946659509053	24.99215683540509	18.641633609180335
33	25.218039975738478	27.10318815926156	24.933594539763103	22.745177325236863
34	22.994067081715315	29.402804018488954	26.203490033952185	21.39963886584354
35	26.422052887330327	26.267629691083886	25.144837106185992	22.16548031539979
36	23.305702154953046	30.24254550778391	24.518778278965677	21.932974058297372
37	25.267887644052788	26.82153140403104	27.159658944344905	20.75092200757127
38	24.138820527478963	27.42981239150289	24.275814469070045	24.155552611948103
39	24.458124472765046	27.997657508174324	25.28949991982543	22.254718099235205
40	23.561215028200532	26.061964486150714	28.336133633581294	22.04068685206746
41	20.540028026241487	28.53238704100058	25.93403375698042	24.99355117577752
42	24.276163054163153	25.208976763317693	28.170207129262327	22.34465305325683
43	23.937338343663072	25.39058959682648	25.95390310728752	24.71816895222293
44	23.21925305186249	25.144837106185992	27.37717604244372	24.258733799507798
45	22.83720378981713	24.102219092702722	26.440179312171896	26.620397805308254
46	24.833899203134475	27.53403933434191	25.61507839678744	22.016983065736177
47	21.24347274413157	26.190940970600334	31.01291856355055	21.552667721717548
48	22.676854646987877	25.411853287506013	27.273297684697813	24.637994380808298
49	22.490710207268698	23.45524515989598	31.0303478182059	23.023696814629417
50	21.645739941577137	24.007055362284486	29.449863006058408	24.897341690079966
51	21.292971827352776	23.927577961056073	27.226587282221466	27.552862929369688
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	212.5
2	345.0
3	2009.5
4	3674.0
5	2451.0
6	1228.0
7	1143.5
8	1059.0
9	1052.5
10	1046.0
11	1031.5
12	1017.0
13	945.0
14	873.0
15	898.5
16	924.0
17	903.5
18	883.0
19	879.5
20	876.0
21	934.0
22	992.0
23	1019.0
24	1046.0
25	1262.0
26	1835.5
27	2193.0
28	2650.5
29	3108.0
30	3577.5
31	4047.0
32	4868.0
33	5689.0
34	6271.0
35	6853.0
36	7273.5
37	7694.0
38	8678.5
39	9663.0
40	10701.5
41	11740.0
42	12706.5
43	13673.0
44	14892.5
45	16112.0
46	17584.5
47	19057.0
48	28934.5
49	38812.0
50	33734.0
51	28656.0
52	28018.0
53	27380.0
54	23365.0
55	19350.0
56	17034.0
57	14718.0
58	13318.0
59	11918.0
60	10934.5
61	9951.0
62	8880.5
63	7810.0
64	6458.5
65	5107.0
66	3981.5
67	2856.0
68	2367.0
69	1878.0
70	1539.0
71	1200.0
72	1027.5
73	855.0
74	672.5
75	390.0
76	290.0
77	212.5
78	135.0
79	85.0
80	35.0
81	48.0
82	61.0
83	38.5
84	16.0
85	8.5
86	1.0
87	2.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	286874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.75610152318901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78759250119805	33.919571841816975
2	8.972411031243851	8.031402780442615
3	3.6962492236259346	4.962891165228326
4	2.370938890219494	4.24455926703763
5	1.7119463484630542	3.831002228703258
6	1.2587692982921812	3.380256390518295
7	1.0453621751599658	3.275043494797278
8	0.8315288156040576	2.977279047250585
9	0.7051423783960028	2.840348148821501
>10	3.5560979776804036	22.979150311469915
>50	0.039780845926421726	1.252937900567059
>100	0.014040298562266492	1.133543985272009
>500	0.00546011610754808	1.5744636198265356
>1k	0.004680099520755498	5.597549818248042
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	4479	1.561312632026604	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC	3347	1.1667143066293912	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG	3252	1.1335987227842188	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2288	0.7975626930289952	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1633	0.5692394570438589	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT	1035	0.3607855713658261	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	895	0.31198365833083513	Illumina PCR Primer Index 8 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT	735	0.2562100434337026	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGGAAGCT	658	0.22936899126445756	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC	609	0.2122883217022107	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTA	558	0.19451048195374973	No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	543	0.18928170555714355	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	512	0.17847556767082412	Illumina PCR Primer Index 8 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	484	0.16871518506382593	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19939067325724882	0.0
2	0.0	0.0	0.0	1.1304614569462552	0.0
3	3.4858509310707836E-4	0.0	0.0	1.5365630904160015	0.0
4	3.4858509310707836E-4	0.0	0.0	2.290204061713505	0.0
5	3.4858509310707836E-4	0.0	0.0	4.949559737027406	0.0
6	3.4858509310707836E-4	0.0	0.0	6.154966988991683	0.0
7	3.4858509310707836E-4	0.0	0.0	7.099981176404972	0.0
8	3.4858509310707836E-4	0.0	0.0	8.35907053270774	0.0
9	3.4858509310707836E-4	0.0	0.0	8.756108953756701	0.0
10	3.4858509310707836E-4	0.0	0.0	10.800909109922824	0.0
11	3.4858509310707836E-4	0.0	0.0	12.069758848832588	0.0
12	3.4858509310707836E-4	0.0	0.0	14.252250116776006	0.0
13	6.971701862141567E-4	0.0	0.0	14.824626839657828	0.0
14	0.0010457552793212352	0.0	0.0	15.079093957625997	0.0
15	0.0010457552793212352	0.0	0.0	15.61556641591779	0.0
16	0.0010457552793212352	0.0	0.0	16.35874983442208	0.0
17	0.0010457552793212352	0.0	0.0	17.20616019576539	0.0
18	0.0010457552793212352	0.0	0.0	18.06995405648473	0.0
19	0.0010457552793212352	0.0	0.0	18.823595027782233	0.0
20	0.0010457552793212352	0.0	0.0	19.38481702768463	0.0
21	0.0010457552793212352	0.0	0.0	20.02412208844301	0.0
22	0.0010457552793212352	0.0	0.0	20.73418992310213	0.0
23	0.0010457552793212352	0.0	0.0	21.428571428571427	0.0
24	0.0010457552793212352	0.0	0.0	21.951797653325155	0.0
25	0.0010457552793212352	0.0	0.0	22.473280952613344	0.0
26	0.0013943403724283134	0.0	0.0	22.994764251901533	0.0
27	0.0013943403724283134	0.0	0.0	23.519384817027685	0.0
28	0.001742925465535392	0.0	0.0	23.992763373467096	0.0
29	0.001742925465535392	0.0	0.0	24.44801550506494	0.0
30	0.001742925465535392	0.0	0.0	25.00052287763966	0.0
31	0.001742925465535392	0.0	0.0	25.492376444013747	0.0
32	0.0020915105586424704	0.0	0.0	25.989110201691336	0.0
33	0.0020915105586424704	0.0	0.0	26.50048453327942	0.0
34	0.0020915105586424704	0.0	0.0	27.001052726981182	0.0
35	0.0020915105586424704	0.0	0.0	27.47861430453788	0.0
36	0.0020915105586424704	0.0	0.0	27.995914582708785	0.0
37	0.0020915105586424704	0.0	0.0	28.483585127965586	0.0
38	0.0020915105586424704	0.0	0.0	28.959055194963643	0.0
39	0.0020915105586424704	0.0	0.0	29.446377155127337	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	80	0.0	45.000004	31
CGACGGT	35	1.2076634E-7	45.000004	28
CGAATAT	20	7.024425E-4	45.000004	14
GTACGAG	20	7.024425E-4	45.000004	1
CACAACG	45	3.8198777E-10	45.0	12
TACACGG	25	3.8834634E-5	45.0	2
GGCGATA	110	0.0	42.954548	8
CGTTTTT	1135	0.0	42.81938	1
CCGATGA	630	0.0	42.5	18
CGATGAA	640	0.0	42.187504	19
CGGAAGG	55	6.002665E-11	40.909092	2
GGGACCG	105	0.0	40.714287	7
ACAACGA	50	1.0750227E-9	40.5	13
CGGGTAT	50	1.0750227E-9	40.5	6
CGAACAC	50	1.0750227E-9	40.5	34
CGCTCGA	50	1.0750227E-9	40.5	41
ACCGATG	675	0.0	40.333332	17
GATGAAT	680	0.0	40.036762	20
GTGCAAG	45	1.9195795E-8	40.0	1
CTCGAAT	85	0.0	39.705883	43
>>END_MODULE