##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935469.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447675
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06345004746747	31.0	30.0	33.0	28.0	34.0
2	31.053391411179984	31.0	31.0	34.0	28.0	34.0
3	30.93376668341989	31.0	30.0	34.0	28.0	34.0
4	34.86536214888033	35.0	35.0	37.0	32.0	37.0
5	34.746898978053274	35.0	35.0	37.0	32.0	37.0
6	34.865183447813706	35.0	35.0	37.0	32.0	37.0
7	35.26198023119451	36.0	35.0	37.0	33.0	37.0
8	34.67489138325794	37.0	35.0	37.0	31.0	37.0
9	36.3769363935891	39.0	35.0	39.0	32.0	39.0
10	36.035961355894344	37.0	35.0	39.0	32.0	39.0
11	36.417707041938904	37.0	35.0	39.0	32.0	39.0
12	36.54482604568046	38.0	35.0	39.0	32.0	39.0
13	36.43972524711007	39.0	35.0	39.0	32.0	39.0
14	37.567416094264814	39.0	36.0	41.0	32.0	41.0
15	37.564165968615626	39.0	36.0	41.0	32.0	41.0
16	37.79424582565477	39.0	36.0	41.0	33.0	41.0
17	37.45000725973083	39.0	36.0	41.0	32.0	41.0
18	37.552771541855144	39.0	36.0	40.0	32.0	41.0
19	37.46571284972357	39.0	36.0	40.0	32.0	41.0
20	37.08138716702965	39.0	35.0	40.0	31.0	41.0
21	37.48036186965991	39.0	36.0	41.0	32.0	41.0
22	37.506378511196736	39.0	36.0	41.0	32.0	41.0
23	37.407963366281344	39.0	36.0	41.0	32.0	41.0
24	37.40428659183559	39.0	35.0	41.0	32.0	41.0
25	37.31206120511532	39.0	35.0	41.0	32.0	41.0
26	37.11971631205674	39.0	35.0	41.0	31.0	41.0
27	37.024678617300495	39.0	35.0	41.0	31.0	41.0
28	36.93509353883956	39.0	35.0	41.0	31.0	41.0
29	36.84238119171274	39.0	35.0	41.0	30.0	41.0
30	36.63585190149104	39.0	35.0	41.0	30.0	41.0
31	36.59628078405093	39.0	35.0	40.0	30.0	41.0
32	36.28180488077288	39.0	35.0	40.0	30.0	41.0
33	36.339565533031774	39.0	35.0	41.0	30.0	41.0
34	36.18723404255319	39.0	35.0	41.0	29.0	41.0
35	36.113604735578264	39.0	35.0	41.0	29.0	41.0
36	36.17577930418272	39.0	35.0	41.0	29.0	41.0
37	36.056326576199254	39.0	35.0	41.0	28.0	41.0
38	35.75265091863517	39.0	35.0	41.0	27.0	41.0
39	35.72088680404311	39.0	35.0	40.0	27.0	41.0
40	35.653690735466576	39.0	35.0	40.0	26.0	41.0
41	35.71329871000167	39.0	35.0	40.0	26.0	41.0
42	35.76342659295248	39.0	35.0	40.0	27.0	41.0
43	35.7446853185905	39.0	35.0	40.0	27.0	41.0
44	35.78022896074161	39.0	35.0	40.0	27.0	41.0
45	35.840699167923155	39.0	35.0	40.0	27.0	41.0
46	35.69872563801865	39.0	35.0	40.0	27.0	41.0
47	35.37769140559558	38.0	35.0	40.0	26.0	41.0
48	35.46249623052437	38.0	35.0	40.0	26.0	41.0
49	35.56264924331267	38.0	35.0	40.0	27.0	41.0
50	35.36729547104484	38.0	35.0	40.0	26.0	41.0
51	34.269023286982744	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	17.0
10	22.0
11	14.0
12	11.0
13	15.0
14	20.0
15	20.0
16	34.0
17	76.0
18	170.0
19	355.0
20	821.0
21	1789.0
22	3308.0
23	4600.0
24	5294.0
25	5332.0
26	5376.0
27	5292.0
28	5438.0
29	6339.0
30	8003.0
31	10680.0
32	13928.0
33	18868.0
34	29650.0
35	33873.0
36	37506.0
37	54521.0
38	93039.0
39	103234.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.09767130172558	17.375998212989334	18.793991176634837	10.732339308650248
2	28.409225442564363	35.75205227006199	20.335734628916065	15.502987658457586
3	27.287652873178086	35.55637460211091	22.755570447311108	14.4004020773999
4	25.256491874685878	20.652482269503547	37.242865918355946	16.848159937454625
5	32.80259116546602	25.631987490925333	21.693192606243368	19.872228737365276
6	20.817334003462335	42.42050594739487	23.770145753057463	12.99201429608533
7	67.12950242921762	5.6976601329089185	22.32758139275144	4.845256045122019
8	64.1697660132909	17.22588931702686	12.116825822304127	6.487518847378121
9	59.21349193052996	7.4478137041380466	12.650918635170605	20.68777573016139
10	40.33975540291506	20.45278382755347	22.32914502708438	16.878315742447086
11	32.91383257943821	21.884626123862176	24.70296532082426	20.498575975875355
12	24.834087228458145	18.422739710727647	30.0371921594907	26.705980901323507
13	26.08365443681242	20.87340146311498	35.19294130786843	17.850002792204165
14	19.44021890880661	32.12241023063606	26.22237113977774	22.21499972077958
15	16.305355447590326	23.33902942983191	37.31948400067013	23.036131121907633
16	17.934662422516332	24.339308650248505	27.747808119729715	29.978220807505444
17	17.71061596023901	28.277210029597367	30.678282235997095	23.333891774166528
18	18.44842798905456	24.85575473278606	29.57726028927235	27.118556988887025
19	19.252806165186797	26.50628245937343	26.43658904339085	27.80432233204892
20	24.305578823923604	25.275032110347905	32.36767744457475	18.05171162115374
21	20.83542748645781	33.01301167141341	27.85502875970291	18.296532082425866
22	18.291841179427042	22.698386105992068	32.3770592505724	26.63271346400849
23	22.51588764170436	28.275423018931146	28.951583179762103	20.25710615960239
24	22.37538392807282	26.2862567710951	25.941363712514658	25.396995588317417
25	18.31149829675546	35.25660356285252	24.208186742614622	22.223711397777404
26	18.30792427542302	24.655386161836155	31.52934606578433	25.507343496956498
27	24.99558831741777	26.489082481711062	25.02038309041157	23.494946110459598
28	16.508181158206288	30.387446250069804	30.280672362763166	22.823700228960742
29	20.19299715195175	27.498296755458757	28.82381191712738	23.48489417546211
30	18.16272965879265	30.562349919026076	27.85770927570224	23.41721114647903
31	20.374155358239797	28.663204333500865	22.411347517730494	28.551292790528844
32	23.916904004020775	31.693974423409838	23.139107611548557	21.25001396102083
33	21.052996035070084	23.99195845200201	26.194449098118056	28.760596414809854
34	19.82129893337801	27.18311274920422	24.705869213156866	28.289719104260904
35	20.379069637571902	23.406935835148264	23.833808008041547	32.380186519238286
36	17.820740492544815	32.87853911878037	23.334115150499805	25.966605238175017
37	17.47964483163009	27.258837326185294	27.260400960518233	28.001116881666388
38	17.632210867258614	28.32076841458647	25.266543809683363	28.78047690847155
39	20.70117831015804	27.93410398168314	25.144580331713858	26.220137376444963
40	18.594962863684593	21.95007538951248	30.96152342659295	28.493438320209975
41	16.67437315016474	25.4854526162953	24.234321773608087	33.60585245993187
42	18.306360641090077	24.1300050259675	29.09588429105936	28.46775004188306
43	21.418663092645335	24.581671971854583	24.32300217791925	29.676662757580836
44	20.523817501535714	23.10917518288937	28.34757357457977	28.019433740995144
45	18.89875467694198	22.39325403473502	27.028759702909475	31.679231585413525
46	25.377338470988995	27.112079075221978	24.785614564136928	22.724967889652092
47	16.735354889149495	24.769308091807673	34.9541520075948	23.541185011448036
48	19.78310158038756	25.8596079745351	25.90137934885799	28.455911096219356
49	18.68051599932987	21.971072764840567	33.73384709889987	25.61456413692969
50	20.516669458870833	21.741888646897863	30.02736360082649	27.714078293404814
51	19.090411570894066	21.74010163623164	26.595409616351144	32.57407717652315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	361.5
2	579.0
3	2039.0
4	3499.0
5	2310.5
6	1122.0
7	1079.5
8	1037.0
9	978.0
10	919.0
11	913.5
12	908.0
13	872.0
14	836.0
15	890.0
16	944.0
17	903.5
18	863.0
19	943.0
20	1023.0
21	1162.0
22	1301.0
23	1445.0
24	1589.0
25	1768.5
26	2635.0
27	3322.0
28	3923.0
29	4524.0
30	5196.5
31	5869.0
32	7015.5
33	8162.0
34	9068.0
35	9974.0
36	10429.5
37	10885.0
38	12470.0
39	14055.0
40	16078.5
41	18102.0
42	19769.0
43	21436.0
44	23401.0
45	25366.0
46	52892.5
47	80419.0
48	62440.5
49	44462.0
50	43490.0
51	42518.0
52	36164.0
53	29810.0
54	26860.5
55	23911.0
56	22711.5
57	21512.0
58	19607.0
59	17702.0
60	16319.0
61	14936.0
62	13312.0
63	11688.0
64	9765.5
65	7843.0
66	6598.5
67	5354.0
68	4380.5
69	3407.0
70	2878.5
71	2350.0
72	1919.5
73	1489.0
74	1192.0
75	700.5
76	506.0
77	368.0
78	230.0
79	174.0
80	118.0
81	101.0
82	84.0
83	48.0
84	12.0
85	9.0
86	6.0
87	4.0
88	2.0
89	2.5
90	3.0
91	4.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	447675.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.88689328209802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35664424069516	25.59185419157962
2	9.564699143171097	6.673652765663689
3	3.784870377930323	3.9612710688428754
4	2.1480060183143834	2.997490269209527
5	1.5361883405020693	2.6796419348149474
6	1.2021495544034848	2.5163557928157636
7	1.0739212824804814	2.6226044022689274
8	0.9290491890325027	2.5929311929277303
9	0.8705310313822427	2.733311087152832
>10	5.424474337933152	30.115485603650477
>50	0.05959122214359754	1.4229526705717965
>100	0.03951157120390707	2.4400576364672477
>500	0.0032386533773694315	0.8428796984647928
>1k	0.00518184540379109	4.333260347925165
>5k	0.0	0.0
>10k+	0.001943192026421659	8.476251337644605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	13410	2.995476629251131	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC	12160	2.7162562126542693	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG	11940	2.6671134193332215	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	4383	0.9790584687552353	TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	2664	0.5950745518512314	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	2555	0.570726531523985	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT	2239	0.5001396102082984	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2094	0.4677500418830625	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC	2015	0.45010331155414085	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTA	1865	0.4165968615625175	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTCGAATGC	1361	0.30401518959066287	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	914	0.20416596861562517	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	913	0.2039425922823477	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT	823	0.18383872228737366	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATG	549	0.1226336069693416	No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	531	0.11861283297034679	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACAATCCCTCGT	497	0.11101803763891216	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACAATCCC	461	0.10297648964092254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.93505333109957E-4	0.0	0.0	0.21555816161277713	0.0
2	8.93505333109957E-4	0.0	0.0	1.5808343106047915	0.0
3	8.93505333109957E-4	0.0	0.0	1.9337689171832244	0.0
4	8.93505333109957E-4	0.0	0.0	2.9309208689339363	0.0
5	8.93505333109957E-4	0.0	0.0	7.017144133579047	0.0
6	8.93505333109957E-4	0.0	0.0	7.878036522030491	0.0
7	8.93505333109957E-4	0.0	0.0	9.086055732395153	0.0
8	8.93505333109957E-4	0.0	0.0	10.467414977383147	0.0
9	8.93505333109957E-4	0.0	0.0	10.81498855196292	0.0
10	8.93505333109957E-4	0.0	0.0	14.367119003741553	0.0
11	8.93505333109957E-4	0.0	0.0	15.554140838778132	0.0
12	8.93505333109957E-4	0.0	0.0	19.30887362483945	0.0
13	8.93505333109957E-4	0.0	0.0	19.89590662869269	0.0
14	8.93505333109957E-4	0.0	0.0	20.1726699056235	0.0
15	8.93505333109957E-4	0.0	0.0	20.94130786843134	0.0
16	8.93505333109957E-4	0.0	0.0	21.711732841905402	0.0
17	8.93505333109957E-4	0.0	0.0	22.474563020048027	0.0
18	0.0011168816663874462	0.0	0.0	23.26687887418328	0.0
19	0.0011168816663874462	0.0	0.0	24.308035963589656	0.0
20	0.0011168816663874462	0.0	0.0	24.8861339141118	0.0
21	0.0011168816663874462	0.0	0.0	25.457530574635616	0.0
22	0.0011168816663874462	0.0	0.0	26.161612777126262	0.0
23	0.0013402579996649355	0.0	0.0	26.77210029597364	0.0
24	0.0013402579996649355	0.0	0.0	27.26888926118278	0.0
25	0.0013402579996649355	0.0	0.0	27.696878315742445	0.0
26	0.0013402579996649355	0.0	0.0	28.120399843636566	0.0
27	0.0013402579996649355	0.0	0.0	28.637292678840677	0.0
28	0.0015636343329424247	0.0	0.0	29.059473948735132	0.0
29	0.0015636343329424247	0.0	0.0	29.47137990729882	0.0
30	0.0015636343329424247	0.0	0.0	29.95834031384375	0.0
31	0.0015636343329424247	0.0	0.0	30.41715530239571	0.0
32	0.0015636343329424247	0.0	0.0	30.85139889428715	0.0
33	0.0015636343329424247	0.0	0.0	31.270229519182443	0.0
34	0.0015636343329424247	0.0	0.0	31.68414586474563	0.0
35	0.0015636343329424247	0.0	0.0	32.12978164963422	0.0
36	0.0015636343329424247	0.0	0.0	32.53565644719942	0.0
37	0.0015636343329424247	0.0	0.0	32.97794158708885	0.0
38	0.0015636343329424247	0.0	0.0	33.40146311498297	0.0
39	0.0015636343329424247	0.0	0.0	33.809124923214384	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.028667E-4	45.0	25
GTACCGG	20	7.028667E-4	45.0	2
TCCGCAC	20	7.028667E-4	45.0	16
GATCGCC	20	7.028667E-4	45.0	38
ATTACGG	25	3.8869763E-5	45.0	2
TAGCACG	20	7.028667E-4	45.0	1
CCCGTAA	40	6.7993824E-9	45.0	41
CGGCTAT	20	7.028667E-4	45.0	31
CGGTCTA	20	7.028667E-4	45.0	31
CGTCATA	45	3.8380676E-10	45.0	38
CTAGGCG	20	7.028667E-4	45.0	1
GCGTAAG	20	7.028667E-4	45.0	1
GCGAGCC	20	7.028667E-4	45.0	33
CGTTCAT	30	2.162522E-6	44.999996	17
CCCCGTA	60	0.0	44.999996	40
TTCGCGG	30	2.162522E-6	44.999996	2
CGATGAA	1630	0.0	43.757668	19
CCGATGA	1625	0.0	43.47692	18
CTCGAAT	285	0.0	43.421047	43
TCGAATG	255	0.0	43.235294	44
>>END_MODULE