FastQCFastQC Report
Sat 14 Jan 2017
SRR2935468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644106
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC120361.8686365287701092No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96911.504566018636684No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC79251.2303875449072048No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG71991.1176731780172828No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT34690.5385759486792546No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC34230.5314342670305819TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT19740.3064712950973908No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG17660.2741784737294793No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT16230.25197715903904017No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC15400.2390910812816524No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT14780.2294653364508326No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT14690.22806805091087493No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC14230.22092636926220216No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT11730.18211288204115472No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA11470.17807627937016576No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC11170.17341866090364008TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT10190.15820377391298948TruSeq Adapter, Index 22 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT10100.15680648837303177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAG351.2108103E-745.0000041
TACGTAG351.2108103E-745.0000041
TTCGCAC207.030975E-445.022
TACGAAT302.1640099E-644.99999612
TACACGG302.1640099E-644.9999962
TAGTACG302.1640099E-644.9999961
CGTTTTT41800.043.815791
CGATGAA8350.043.38323219
CTCGAAT1250.043.19999743
CCGATGA8550.042.6315818
CGGGATC603.6379788E-1241.2499966
CACCGAT9550.041.23036616
TACGGGT556.002665E-1140.9090924
CTAGCGG501.0804797E-940.5000042
CCCTCGA1500.040.541
ACCGATG9100.040.30219717
TACGGCT9500.040.0263147
GTACGAG451.9261279E-840.01
GATGAAT9250.039.40540320
CCACCGA11650.039.01287515
Produced by FastQC (version 0.11.5)