##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935468.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644106
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10749162404946	31.0	31.0	33.0	28.0	34.0
2	31.156171499722095	31.0	31.0	34.0	28.0	34.0
3	30.950756552492912	31.0	30.0	34.0	27.0	34.0
4	34.76678372814413	35.0	35.0	37.0	32.0	37.0
5	34.77232784665878	35.0	35.0	37.0	32.0	37.0
6	34.94305129900979	36.0	35.0	37.0	32.0	37.0
7	35.297578348905304	37.0	35.0	37.0	33.0	37.0
8	35.013264897392666	37.0	35.0	37.0	32.0	37.0
9	36.815339400657656	39.0	37.0	39.0	32.0	39.0
10	36.27093677127678	37.0	35.0	39.0	32.0	39.0
11	36.623475018087085	38.0	35.0	39.0	32.0	39.0
12	36.76307936892375	39.0	35.0	39.0	33.0	39.0
13	36.78054078055475	39.0	35.0	39.0	32.0	39.0
14	37.907970116719916	40.0	37.0	41.0	33.0	41.0
15	37.997244242407305	40.0	37.0	41.0	33.0	41.0
16	38.034753596457726	40.0	37.0	41.0	33.0	41.0
17	37.84684508450472	40.0	37.0	41.0	33.0	41.0
18	37.77716400716652	39.0	37.0	41.0	33.0	41.0
19	37.59202367312213	39.0	37.0	40.0	33.0	41.0
20	37.4154859603854	39.0	35.0	41.0	32.0	41.0
21	37.52362965102017	39.0	35.0	41.0	33.0	41.0
22	37.618730457409185	39.0	35.0	41.0	33.0	41.0
23	37.62865584236135	39.0	35.0	41.0	33.0	41.0
24	37.600495881112735	39.0	35.0	41.0	33.0	41.0
25	37.308244295193646	39.0	35.0	41.0	33.0	41.0
26	37.283458002254285	39.0	35.0	41.0	32.0	41.0
27	37.29488158781319	39.0	35.0	41.0	33.0	41.0
28	37.13214905621125	39.0	35.0	41.0	32.0	41.0
29	37.08830223596737	39.0	35.0	41.0	32.0	41.0
30	36.753307685380975	39.0	35.0	41.0	31.0	41.0
31	36.74416167525221	39.0	35.0	41.0	31.0	41.0
32	36.509052857759436	39.0	35.0	41.0	30.0	41.0
33	36.3031581758282	39.0	35.0	41.0	30.0	41.0
34	36.13917584993774	39.0	35.0	41.0	29.0	41.0
35	36.038631840100855	39.0	35.0	41.0	28.0	41.0
36	35.87543354665226	39.0	35.0	41.0	27.0	41.0
37	35.83535318720832	39.0	35.0	41.0	27.0	41.0
38	35.753141563655674	39.0	35.0	41.0	26.0	41.0
39	35.61841373935346	39.0	35.0	41.0	26.0	41.0
40	35.500034155868754	39.0	35.0	41.0	25.0	41.0
41	35.355571908971505	39.0	35.0	40.0	24.0	41.0
42	35.44815139123064	39.0	35.0	40.0	24.0	41.0
43	35.4386948732041	39.0	35.0	40.0	25.0	41.0
44	35.43177209962335	39.0	35.0	40.0	25.0	41.0
45	35.460046948794144	39.0	35.0	40.0	25.0	41.0
46	35.28173778850065	38.0	35.0	40.0	24.0	41.0
47	35.16208512263509	38.0	35.0	40.0	24.0	41.0
48	35.1398496520759	38.0	35.0	40.0	24.0	41.0
49	35.13404625946661	38.0	35.0	40.0	24.0	41.0
50	35.01092366784349	38.0	34.0	40.0	24.0	41.0
51	33.904034429115704	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	14.0
9	22.0
10	18.0
11	25.0
12	24.0
13	18.0
14	27.0
15	38.0
16	71.0
17	178.0
18	264.0
19	577.0
20	1236.0
21	2134.0
22	3457.0
23	4638.0
24	6104.0
25	8117.0
26	9796.0
27	10421.0
28	9885.0
29	10474.0
30	12068.0
31	14854.0
32	18874.0
33	25597.0
34	40780.0
35	48249.0
36	51497.0
37	76493.0
38	134201.0
39	153936.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74231570579998	18.158036099648196	19.563860606794535	16.535787587757294
2	35.36157092155639	27.910002390910815	21.06190595957808	15.666520727954717
3	28.61827090572049	27.809397832033856	28.826776959071953	14.745554303173702
4	25.260749007150995	22.23655733683586	35.24528571384214	17.25740794217101
5	26.932368274787073	27.027538945453077	27.414587040021367	18.62550573973849
6	22.030380092717657	35.72315736850767	28.704281593402325	13.542180945372346
7	69.69427392385725	5.603736031025949	19.78276867472124	4.919221370395556
8	69.4277028936231	9.63273125851956	15.255253017360495	5.684312830496844
9	63.41906456390718	7.030985583118307	16.734046880482406	12.815902972492104
10	36.71352230843992	21.164528819790533	25.779452450373075	16.342496421396476
11	29.169888186106014	22.935044852865833	28.067429895079382	19.82763706594877
12	23.905537287340902	19.559824004123545	33.39512440498924	23.13951430354631
13	23.745315212092418	20.769252265931385	36.82188956476108	18.663542957215117
14	19.602053078220045	27.369873902742714	32.50334572259845	20.524727296438787
15	17.946269713370157	23.998379148773648	37.851533753761025	20.203817384095167
16	19.72905080840731	24.36151813521377	32.05931942878967	23.85011162758925
17	19.49585937718326	25.974761918069383	32.56358425476552	21.965794449981836
18	20.221671588216847	24.935957746085272	31.90887835232089	22.93349231337699
19	20.60375155642084	26.12132164581606	29.704738040012046	23.570188757751055
20	22.903994063088994	25.327818713068968	32.87083181960733	18.897355404234705
21	21.424889692069318	28.46115390944969	31.70394313979376	18.410013258687236
22	20.136902932126077	23.02679993665639	33.29032798949241	23.54596914172512
23	21.150245456493185	26.208419111140092	32.11350305695026	20.52783237541647
24	21.582627704135653	24.651843019627204	30.994122085495245	22.771407190741897
25	20.26964505842206	29.220811481340025	28.854412161973343	21.65513129826457
26	20.193104861622153	23.996205593489268	32.15417959155791	23.656509953330662
27	23.371618957128174	24.652464035422742	29.707377357143077	22.268539650306003
28	19.28844010147398	25.771689752928868	31.88403772049942	23.055832425097734
29	23.088435754363413	23.78692327039338	31.063831108544242	22.06080986669896
30	20.443839989070124	26.32470431885435	32.82021903227108	20.41123665980444
31	21.48869906506072	24.153167335811187	28.956569260339137	25.40156433878896
32	22.943273312156695	28.367691032221405	27.739223047138207	20.949812608483697
33	20.78027529630216	26.938733686691318	28.178591722480462	24.102399294526055
34	21.409985312976435	24.982378676801645	30.876750100138793	22.730885910083124
35	20.269024042626523	24.470040645483817	30.006706970591797	25.254228341297864
36	20.584810574656967	26.33370904788963	27.809397832033856	25.272082545419543
37	18.755608548903442	26.32097822408113	32.021592719210815	22.901820507804615
38	18.81600233501939	26.95876144609738	27.23775279224227	26.987483426640956
39	21.227251415139744	26.472661332140984	29.239286701257246	23.060800551462027
40	20.85029482724893	24.831161330588444	30.86929791059236	23.44924593157027
41	18.6362182622115	25.257488674224426	30.336311104072934	25.76998195949114
42	20.43343797449488	24.18592591902575	30.418129935134903	24.96250617134447
43	21.962999878901922	23.90802135052305	28.415198740579967	25.713780029995064
44	21.034891772472232	23.642071336084435	30.09970408597343	25.223332805469905
45	20.66383483463902	22.978050196706754	28.99383020807134	27.364284760582887
46	23.481228245040413	25.644071006946064	28.13325756940628	22.74144317860725
47	18.92902720980708	24.702300553014567	33.4632808885494	22.905391348628953
48	20.08613489084095	24.012196750224344	30.412696046923955	25.488972312010755
49	19.612765600693052	22.434506121663205	33.837132397462526	24.11559588018121
50	19.53296507096658	22.70635578615942	32.50381148444511	25.256867658428895
51	19.396496849897378	21.898414236166097	30.460980025026934	28.24410888890959
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278.0
1	513.5
2	749.0
3	7672.5
4	14596.0
5	9297.5
6	3999.0
7	3857.0
8	3715.0
9	3687.5
10	3660.0
11	3541.5
12	3423.0
13	3355.0
14	3287.0
15	3143.0
16	2999.0
17	2900.0
18	2801.0
19	2809.0
20	2817.0
21	2808.0
22	2799.0
23	2801.0
24	2803.0
25	3132.0
26	4021.5
27	4582.0
28	5384.5
29	6187.0
30	7552.0
31	8917.0
32	10184.5
33	11452.0
34	13332.5
35	15213.0
36	16521.0
37	17829.0
38	19249.5
39	20670.0
40	22903.5
41	25137.0
42	27430.5
43	29724.0
44	33154.0
45	36584.0
46	61340.5
47	86097.0
48	73560.5
49	61024.0
50	60221.0
51	59418.0
52	52047.0
53	44676.0
54	40083.0
55	35490.0
56	32920.0
57	30350.0
58	27975.0
59	25600.0
60	24086.5
61	22573.0
62	20556.0
63	18539.0
64	15406.5
65	12274.0
66	10088.0
67	7902.0
68	6373.5
69	4845.0
70	3953.0
71	3061.0
72	2554.0
73	2047.0
74	1642.5
75	955.5
76	673.0
77	500.5
78	328.0
79	238.0
80	148.0
81	112.5
82	77.0
83	61.5
84	46.0
85	27.5
86	9.0
87	6.5
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	644106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.49130142476418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22666255040298	28.57080841981816
2	9.105167263808337	7.009394753482839
3	3.6898482845930114	4.260811876017559
4	2.221230637954221	3.419922320776686
5	1.640018640776787	3.1563225922185674
6	1.2578747594294868	2.905034191188186
7	1.0573087451485657	2.8488032725986825
8	0.9060956324199015	2.79014400857094
9	0.7802772682329459	2.70304987738013
>10	5.041316150696041	30.03740117257404
>50	0.04362311608052368	1.1342626880095161
>100	0.022423097001971933	1.5036669974952712
>500	8.153853455262521E-4	0.169166460373607
>1k	0.005707697418683764	3.7083233998967597
>5k	0.001223078018289378	3.8941240391197196
>10k+	4.0769267276312605E-4	1.8887639304793449
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	12036	1.8686365287701092	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9691	1.504566018636684	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	7925	1.2303875449072048	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	7199	1.1176731780172828	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	3469	0.5385759486792546	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	3423	0.5314342670305819	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	1974	0.3064712950973908	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	1766	0.2741784737294793	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	1623	0.25197715903904017	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	1540	0.2390910812816524	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT	1478	0.2294653364508326	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	1469	0.22806805091087493	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC	1423	0.22092636926220216	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	1173	0.18211288204115472	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA	1147	0.17807627937016576	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1117	0.17341866090364008	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	1019	0.15820377391298948	TruSeq Adapter, Index 22 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	1010	0.15680648837303177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.105078977683797E-4	0.0	0.0	0.16612172530608316	0.0
2	3.105078977683797E-4	0.0	0.0	0.9839995280279954	0.0
3	3.105078977683797E-4	0.0	0.0	1.4565925484314695	0.0
4	3.105078977683797E-4	0.0	0.0	2.450838837085821	0.0
5	3.105078977683797E-4	0.0	0.0	5.405942500147491	0.0
6	3.105078977683797E-4	0.0	0.0	6.846233383946121	0.0
7	3.105078977683797E-4	0.0	0.0	7.9741533225897605	0.0
8	3.105078977683797E-4	0.0	0.0	9.292104094667648	0.0
9	3.105078977683797E-4	0.0	0.0	9.85691796070833	0.0
10	3.105078977683797E-4	0.0	0.0	11.91931141768591	0.0
11	3.105078977683797E-4	0.0	0.0	12.9779880951272	0.0
12	3.105078977683797E-4	0.0	0.0	15.150456601863668	0.0
13	3.105078977683797E-4	0.0	0.0	15.679562059660988	0.0
14	4.657618466525696E-4	0.0	0.0	15.960571707141371	0.0
15	4.657618466525696E-4	0.0	0.0	16.444343011864508	0.0
16	4.657618466525696E-4	0.0	0.0	17.01769584509382	0.0
17	4.657618466525696E-4	0.0	0.0	17.643058751199337	0.0
18	4.657618466525696E-4	0.0	0.0	18.30707989057702	0.0
19	4.657618466525696E-4	0.0	0.0	18.987713202485306	0.0
20	4.657618466525696E-4	0.0	0.0	19.448506922773582	0.0
21	4.657618466525696E-4	0.0	0.0	19.95277174874943	0.0
22	4.657618466525696E-4	0.0	0.0	20.54677335718034	0.0
23	4.657618466525696E-4	0.0	0.0	21.09637233623037	0.0
24	4.657618466525696E-4	0.0	0.0	21.54909285117667	0.0
25	4.657618466525696E-4	0.0	0.0	21.95259786432668	0.0
26	4.657618466525696E-4	0.0	0.0	22.367591669694118	0.0
27	4.657618466525696E-4	0.0	0.0	22.816120328020542	0.0
28	6.210157955367595E-4	0.0	0.0	23.232666672876825	0.0
29	6.210157955367595E-4	0.0	0.0	23.66085706389942	0.0
30	6.210157955367595E-4	0.0	0.0	24.170555778086214	0.0
31	6.210157955367595E-4	0.0	0.0	24.584773313709235	0.0
32	6.210157955367595E-4	0.0	0.0	25.00194067436105	0.0
33	6.210157955367595E-4	0.0	0.0	25.427181240354848	0.0
34	6.210157955367595E-4	0.0	0.0	25.847608933933234	0.0
35	6.210157955367595E-4	0.0	0.0	26.28061219737124	0.0
36	6.210157955367595E-4	0.0	0.0	26.698711081716365	0.0
37	6.210157955367595E-4	0.0	0.0	27.118673013448095	0.0
38	6.210157955367595E-4	0.0	0.0	27.572480306036585	0.0
39	6.210157955367595E-4	0.0	0.0	28.015264568254292	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACAG	35	1.2108103E-7	45.000004	1
TACGTAG	35	1.2108103E-7	45.000004	1
TTCGCAC	20	7.030975E-4	45.0	22
TACGAAT	30	2.1640099E-6	44.999996	12
TACACGG	30	2.1640099E-6	44.999996	2
TAGTACG	30	2.1640099E-6	44.999996	1
CGTTTTT	4180	0.0	43.81579	1
CGATGAA	835	0.0	43.383232	19
CTCGAAT	125	0.0	43.199997	43
CCGATGA	855	0.0	42.63158	18
CGGGATC	60	3.6379788E-12	41.249996	6
CACCGAT	955	0.0	41.230366	16
TACGGGT	55	6.002665E-11	40.909092	4
CTAGCGG	50	1.0804797E-9	40.500004	2
CCCTCGA	150	0.0	40.5	41
ACCGATG	910	0.0	40.302197	17
TACGGCT	950	0.0	40.026314	7
GTACGAG	45	1.9261279E-8	40.0	1
GATGAAT	925	0.0	39.405403	20
CCACCGA	1165	0.0	39.012875	15
>>END_MODULE