##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	437500
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234098285714285	31.0	31.0	33.0	28.0	34.0
2	31.242678857142856	31.0	31.0	34.0	28.0	34.0
3	31.033090285714287	31.0	31.0	34.0	27.0	34.0
4	34.846272	35.0	35.0	37.0	32.0	37.0
5	34.86401828571429	35.0	35.0	37.0	33.0	37.0
6	35.052848	37.0	35.0	37.0	32.0	37.0
7	35.46052114285714	37.0	35.0	37.0	33.0	37.0
8	35.13392685714286	37.0	35.0	37.0	32.0	37.0
9	37.04136457142857	39.0	37.0	39.0	33.0	39.0
10	36.42021942857143	38.0	35.0	39.0	32.0	39.0
11	36.74366628571428	39.0	37.0	39.0	32.0	39.0
12	36.90358857142857	39.0	37.0	39.0	33.0	39.0
13	36.97106514285714	39.0	37.0	39.0	33.0	39.0
14	38.072512	40.0	37.0	41.0	33.0	41.0
15	38.20074057142857	40.0	37.0	41.0	33.0	41.0
16	38.195563428571425	40.0	37.0	41.0	34.0	41.0
17	38.069792	40.0	37.0	41.0	33.0	41.0
18	38.03892571428572	39.0	37.0	41.0	34.0	41.0
19	37.89936914285714	39.0	37.0	41.0	34.0	41.0
20	37.88928457142857	39.0	36.0	41.0	34.0	41.0
21	37.91507657142857	40.0	36.0	41.0	33.0	41.0
22	38.009712	40.0	37.0	41.0	34.0	41.0
23	38.05112914285714	40.0	37.0	41.0	34.0	41.0
24	38.02343771428571	40.0	37.0	41.0	34.0	41.0
25	37.65502857142857	39.0	36.0	41.0	33.0	41.0
26	37.73008228571429	40.0	36.0	41.0	33.0	41.0
27	37.778100571428574	40.0	36.0	41.0	33.0	41.0
28	37.713188571428574	40.0	36.0	41.0	33.0	41.0
29	37.70229257142857	40.0	36.0	41.0	33.0	41.0
30	37.43533942857143	40.0	36.0	41.0	33.0	41.0
31	37.422724571428574	40.0	36.0	41.0	33.0	41.0
32	37.342304	40.0	36.0	41.0	33.0	41.0
33	37.274304	40.0	36.0	41.0	32.0	41.0
34	37.14955428571429	40.0	36.0	41.0	32.0	41.0
35	37.10166857142857	40.0	36.0	41.0	32.0	41.0
36	36.96354514285714	40.0	36.0	41.0	31.0	41.0
37	36.96512	40.0	36.0	41.0	31.0	41.0
38	36.822882285714286	40.0	35.0	41.0	31.0	41.0
39	36.745808	39.0	35.0	41.0	31.0	41.0
40	36.60131657142857	39.0	35.0	41.0	30.0	41.0
41	36.450534857142856	39.0	35.0	41.0	30.0	41.0
42	36.491065142857146	39.0	35.0	41.0	30.0	41.0
43	36.48885257142857	39.0	35.0	41.0	30.0	41.0
44	36.51538057142857	39.0	35.0	41.0	31.0	41.0
45	36.496736	39.0	35.0	41.0	30.0	41.0
46	36.38893028571429	39.0	35.0	41.0	30.0	41.0
47	36.32390857142857	39.0	35.0	41.0	30.0	41.0
48	36.252601142857145	39.0	35.0	41.0	30.0	41.0
49	36.247654857142855	39.0	35.0	41.0	30.0	41.0
50	36.14710171428572	39.0	35.0	41.0	30.0	41.0
51	34.959801142857145	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	22.0
10	21.0
11	17.0
12	14.0
13	15.0
14	17.0
15	21.0
16	38.0
17	63.0
18	119.0
19	206.0
20	471.0
21	837.0
22	1263.0
23	1893.0
24	2450.0
25	3441.0
26	4427.0
27	4759.0
28	5016.0
29	5444.0
30	6337.0
31	8344.0
32	11149.0
33	15552.0
34	26273.0
35	31822.0
36	35443.0
37	54179.0
38	98299.0
39	119524.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.017828571428566	19.65554285714286	22.751314285714287	13.575314285714285
2	34.8144	25.769828571428572	23.03748571428571	16.378285714285713
3	31.068800000000003	25.595885714285714	27.63771428571429	15.697600000000001
4	27.914742857142855	24.032	30.007314285714287	18.045942857142858
5	26.143542857142858	28.958628571428573	25.670628571428573	19.2272
6	23.384457142857144	35.68822857142857	26.58674285714286	14.340571428571428
7	74.98651428571429	5.905371428571429	14.144000000000002	4.9641142857142855
8	74.98354285714287	8.086857142857143	11.5856	5.344
9	68.58537142857143	7.937142857142857	13.8432	9.634285714285715
10	37.06605714285715	24.821714285714286	22.3872	15.72502857142857
11	28.47062857142857	24.976000000000003	26.944228571428567	19.609142857142857
12	24.8704	21.47725714285714	32.04228571428571	21.61005714285714
13	24.15977142857143	23.059885714285713	33.645714285714284	19.13462857142857
14	19.63977142857143	28.130285714285712	30.42674285714286	21.8032
15	18.386514285714288	26.22674285714286	35.082742857142854	20.304
16	20.565485714285714	25.134857142857143	31.99314285714286	22.306514285714286
17	20.019885714285714	26.327542857142856	30.564342857142858	23.08822857142857
18	20.79497142857143	25.743771428571428	31.058285714285716	22.402971428571426
19	21.492800000000003	27.464228571428574	28.566399999999998	22.47657142857143
20	23.378285714285717	26.095085714285716	30.662628571428574	19.864
21	22.573485714285717	27.86994285714286	30.17142857142857	19.385142857142856
22	20.3248	25.0672	30.126399999999997	24.4816
23	20.542628571428573	27.071085714285715	30.519542857142856	21.866742857142857
24	21.1792	25.8528	30.10034285714286	22.86765714285714
25	20.189714285714285	28.68045714285714	28.1872	22.94262857142857
26	19.380114285714285	26.788114285714286	30.83954285714286	22.992228571428573
27	22.110857142857142	27.381028571428573	28.807314285714288	21.7008
28	19.171657142857143	26.60617142857143	31.232914285714287	22.98925714285714
29	19.864228571428573	25.3888	31.278628571428573	23.468342857142858
30	20.599314285714286	28.110400000000002	29.230171428571428	22.060114285714285
31	21.13942857142857	26.543542857142853	27.032	25.28502857142857
32	21.658057142857142	27.479771428571432	27.962285714285713	22.899885714285713
33	20.773485714285712	28.10834285714286	26.61874285714286	24.499428571428574
34	21.2592	26.252571428571432	29.164114285714287	23.324114285714288
35	20.81417142857143	25.803199999999997	29.408228571428573	23.974400000000003
36	19.864228571428573	29.745371428571428	28.12662857142857	22.263771428571427
37	20.162742857142856	27.978285714285718	29.30102857142857	22.55794285714286
38	20.03382857142857	27.66697142857143	27.209371428571426	25.089828571428573
39	22.083657142857145	26.325257142857144	27.84114285714286	23.74994285714286
40	21.688914285714286	25.690057142857142	31.736685714285713	20.884342857142858
41	19.969142857142856	27.26422857142857	29.789714285714286	22.976914285714287
42	19.920914285714286	26.637485714285713	31.186514285714285	22.255085714285713
43	21.216228571428573	26.826285714285714	28.669942857142853	23.287542857142856
44	20.67474285714286	25.57302857142857	28.917942857142858	24.834285714285713
45	20.551771428571428	24.388571428571428	28.33074285714286	26.728914285714282
46	22.3504	26.5152	28.1216	23.0128
47	20.088	25.31657142857143	32.2448	22.350628571428572
48	19.687314285714287	25.257600000000004	30.20937142857143	24.845714285714283
49	19.840914285714288	24.3824	32.009142857142855	23.767542857142857
50	19.560914285714286	24.015771428571426	31.203657142857143	25.219657142857145
51	19.126857142857144	23.834285714285713	29.434057142857146	27.6048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	380.5
2	596.0
3	3429.0
4	6262.0
5	3979.5
6	1697.0
7	1661.5
8	1626.0
9	1594.0
10	1562.0
11	1509.0
12	1456.0
13	1429.0
14	1402.0
15	1393.0
16	1384.0
17	1365.5
18	1347.0
19	1317.0
20	1287.0
21	1438.5
22	1590.0
23	1757.5
24	1925.0
25	2364.0
26	3334.0
27	3865.0
28	4719.0
29	5573.0
30	6482.0
31	7391.0
32	8007.5
33	8624.0
34	10190.5
35	11757.0
36	12626.0
37	13495.0
38	14870.0
39	16245.0
40	18309.5
41	20374.0
42	23384.0
43	26394.0
44	37511.0
45	48628.0
46	46161.0
47	43694.0
48	43448.0
49	43202.0
50	37733.0
51	32264.0
52	28774.5
53	25285.0
54	22882.0
55	20479.0
56	19424.5
57	18370.0
58	17801.0
59	17232.0
60	16164.5
61	15097.0
62	13483.0
63	11869.0
64	9956.5
65	8044.0
66	6603.0
67	5162.0
68	4170.0
69	3178.0
70	2866.0
71	2554.0
72	2087.0
73	1620.0
74	1286.0
75	770.5
76	589.0
77	397.5
78	206.0
79	173.0
80	140.0
81	97.5
82	55.0
83	49.5
84	44.0
85	26.0
86	8.0
87	6.0
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	437500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.36442944494944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55490345108086	28.218919037790602
2	9.35887711188639	7.18095961285835
3	3.7452509722494414	4.31053250035476
4	2.212736356066627	3.395614712503705
5	1.587722364744936	3.0456031320212693
6	1.2720501901262633	2.928088786172013
7	1.0266249858126044	2.7570117284240916
8	0.8932677689555215	2.74157666380332
9	0.7497715021815979	2.5888100305751705
>10	5.515114613716901	34.277385436986165
>50	0.04575346706182684	1.2511172045474632
>100	0.030100965172254506	2.219537813494761
>500	0.00361211582067054	0.9245379674734161
>1k	0.004214135124115631	4.160305372994915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	4393	1.0041142857142857	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4220	0.9645714285714285	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	2954	0.6752	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	2499	0.5712	No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1758	0.4018285714285715	TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	1169	0.2672	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1020	0.23314285714285715	TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	831	0.18994285714285714	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	827	0.18902857142857143	TruSeq Adapter, Index 15 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	626	0.1430857142857143	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	611	0.13965714285714287	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC	565	0.12914285714285714	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	543	0.1241142857142857	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	500	0.1142857142857143	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT	497	0.1136	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	483	0.1104	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA	476	0.1088	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19451428571428572	0.0
2	0.0	0.0	0.0	0.9766857142857143	0.0
3	0.0	0.0	0.0	1.3945142857142858	0.0
4	0.0	0.0	0.0	2.120457142857143	0.0
5	0.0	0.0	0.0	4.2	0.0
6	0.0	0.0	0.0	5.336	0.0
7	0.0	0.0	0.0	6.2708571428571425	0.0
8	0.0	0.0	0.0	7.662171428571429	0.0
9	0.0	0.0	0.0	8.222857142857142	0.0
10	0.0	0.0	0.0	9.830857142857143	0.0
11	0.0	0.0	0.0	11.130285714285714	0.0
12	0.0	0.0	0.0	12.893257142857143	0.0
13	0.0	0.0	0.0	13.495085714285715	0.0
14	0.0	0.0	0.0	13.765942857142857	0.0
15	0.0	0.0	0.0	14.2128	0.0
16	0.0	0.0	0.0	14.904228571428572	0.0
17	0.0	0.0	0.0	15.664685714285714	0.0
18	0.0	0.0	0.0	16.50057142857143	0.0
19	0.0	0.0	0.0	17.135314285714287	0.0
20	0.0	0.0	0.0	17.645942857142856	0.0
21	0.0	0.0	0.0	18.259657142857144	0.0
22	0.0	0.0	0.0	18.927314285714285	0.0
23	0.0	0.0	0.0	19.576228571428572	0.0
24	4.5714285714285713E-4	0.0	0.0	20.10902857142857	0.0
25	6.857142857142857E-4	0.0	0.0	20.62034285714286	0.0
26	6.857142857142857E-4	0.0	0.0	21.052342857142857	0.0
27	6.857142857142857E-4	0.0	0.0	21.520685714285715	0.0
28	6.857142857142857E-4	0.0	0.0	21.978285714285715	0.0
29	6.857142857142857E-4	0.0	0.0	22.463085714285715	0.0
30	6.857142857142857E-4	0.0	0.0	23.04114285714286	0.0
31	6.857142857142857E-4	0.0	0.0	23.567542857142858	0.0
32	6.857142857142857E-4	0.0	0.0	24.04274285714286	0.0
33	6.857142857142857E-4	0.0	0.0	24.516571428571428	0.0
34	6.857142857142857E-4	0.0	0.0	25.0064	0.0
35	6.857142857142857E-4	0.0	0.0	25.515657142857144	0.0
36	6.857142857142857E-4	0.0	0.0	25.999771428571428	0.0
37	6.857142857142857E-4	0.0	0.0	26.477942857142857	0.0
38	6.857142857142857E-4	0.0	0.0	26.976	0.0
39	6.857142857142857E-4	0.0	0.0	27.481828571428572	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCGTC	25	3.8868307E-5	45.000004	30
ATACGCG	25	3.8868307E-5	45.000004	1
CCGCAGG	25	3.8868307E-5	45.000004	2
CTGAACG	20	7.028491E-4	45.0	1
TCCCGTG	20	7.028491E-4	45.0	28
TAGTACG	20	7.028491E-4	45.0	1
GCGCGAG	30	2.1624073E-6	44.999996	1
CGTTTTT	1620	0.0	42.36111	1
GGCGAAC	75	0.0	42.000004	32
TACGAAT	110	0.0	40.909092	12
ATCCTGC	95	0.0	40.263157	20
TCGGGCA	45	1.9235813E-8	40.0	5
CCGATGA	365	0.0	39.452057	18
CGTATGG	40	3.451678E-7	39.375	2
AACGGGA	80	0.0	39.375	4
CTACGAA	115	0.0	39.130436	11
CGAATAT	115	0.0	39.130436	14
GCGAACC	75	0.0	39.000004	33
TACGCGG	75	0.0	39.000004	2
CGTCTGG	35	6.239423E-6	38.571426	2
>>END_MODULE