##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935465.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504397
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193375456237845	31.0	31.0	33.0	28.0	34.0
2	31.21146041709209	31.0	31.0	34.0	28.0	34.0
3	30.992329454774712	31.0	31.0	34.0	27.0	34.0
4	34.82029433164749	35.0	35.0	37.0	32.0	37.0
5	34.841271855304456	35.0	35.0	37.0	33.0	37.0
6	35.02805726441672	36.0	35.0	37.0	32.0	37.0
7	35.44620804643961	37.0	35.0	37.0	33.0	37.0
8	35.189382569682216	37.0	35.0	37.0	32.0	37.0
9	37.07311106132669	39.0	37.0	39.0	33.0	39.0
10	36.334095960126646	37.0	35.0	39.0	32.0	39.0
11	36.64893922842523	38.0	35.0	39.0	32.0	39.0
12	36.75645176319447	39.0	35.0	39.0	33.0	39.0
13	36.79230050932103	39.0	35.0	39.0	33.0	39.0
14	37.775226656780276	40.0	37.0	41.0	33.0	41.0
15	37.970891975963376	40.0	37.0	41.0	33.0	41.0
16	37.961988275108695	40.0	37.0	41.0	33.0	41.0
17	37.868169319008636	39.0	37.0	41.0	33.0	41.0
18	37.84793723991221	39.0	37.0	41.0	33.0	41.0
19	37.70842808343428	39.0	37.0	41.0	33.0	41.0
20	37.71435397117747	39.0	36.0	41.0	33.0	41.0
21	37.70122542362464	39.0	36.0	41.0	33.0	41.0
22	37.818914466184374	39.0	36.0	41.0	33.0	41.0
23	37.87891284048081	40.0	36.0	41.0	34.0	41.0
24	37.863177219531444	40.0	36.0	41.0	33.0	41.0
25	37.4784742970319	39.0	35.0	41.0	33.0	41.0
26	37.55291367712338	39.0	35.0	41.0	33.0	41.0
27	37.598114183867075	39.0	36.0	41.0	33.0	41.0
28	37.49522300886009	39.0	35.0	41.0	33.0	41.0
29	37.47743939793456	40.0	35.0	41.0	33.0	41.0
30	37.19350234041836	39.0	35.0	41.0	32.0	41.0
31	37.16845857528891	39.0	35.0	41.0	32.0	41.0
32	37.116977301609644	39.0	35.0	41.0	32.0	41.0
33	37.067595564604865	39.0	35.0	41.0	32.0	41.0
34	36.88856198589603	39.0	35.0	41.0	31.0	41.0
35	36.90719611734408	40.0	35.0	41.0	31.0	41.0
36	36.74722688675785	39.0	35.0	41.0	31.0	41.0
37	36.75529592761257	39.0	35.0	41.0	31.0	41.0
38	36.59042976068454	39.0	35.0	41.0	31.0	41.0
39	36.48604373142584	39.0	35.0	41.0	30.0	41.0
40	36.37731984924573	39.0	35.0	41.0	30.0	41.0
41	36.20301468882646	39.0	35.0	41.0	30.0	41.0
42	36.26241829352673	39.0	35.0	41.0	30.0	41.0
43	36.246407095997796	39.0	35.0	41.0	30.0	41.0
44	36.28371699276562	39.0	35.0	41.0	30.0	41.0
45	36.27879230050932	39.0	35.0	41.0	30.0	41.0
46	36.15175942759374	39.0	35.0	41.0	30.0	41.0
47	36.088485855387724	39.0	35.0	41.0	30.0	41.0
48	36.03429044978459	39.0	35.0	40.0	29.0	41.0
49	36.02468690337175	39.0	35.0	40.0	29.0	41.0
50	35.90738446104953	39.0	35.0	40.0	29.0	41.0
51	34.679694764243244	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	15.0
9	25.0
10	25.0
11	25.0
12	21.0
13	18.0
14	22.0
15	20.0
16	57.0
17	101.0
18	179.0
19	334.0
20	638.0
21	1088.0
22	1671.0
23	2322.0
24	3197.0
25	4202.0
26	5324.0
27	5830.0
28	5905.0
29	6754.0
30	8071.0
31	10528.0
32	14245.0
33	20102.0
34	34177.0
35	36490.0
36	40502.0
37	62577.0
38	111353.0
39	128557.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.073418358951386	20.144053196192683	23.418458079647582	12.364070365208358
2	35.439941157461284	24.316163656802082	23.618102407429067	16.625792778307563
3	31.881038150504466	24.741225661532486	27.4569436376505	15.920792550312552
4	27.668285100823358	24.37207199884218	29.149261395289823	18.81038150504464
5	25.80923359972403	29.04160809838282	25.9581242552989	19.19103404659425
6	23.848079984615293	35.16733842588279	26.670459974980027	14.314121614521893
7	76.48697355456119	5.8497572348764955	13.091275324793763	4.571993885768552
8	76.95565199634416	7.065664545982629	11.01215907311106	4.9665243845621605
9	70.95839190161718	7.284341500841599	12.881916426941476	8.875350170599745
10	38.956813779622	23.521749732849322	22.03205015097235	15.489386336556324
11	30.261084027065984	24.391501138983777	26.044564103275793	19.302850730674447
12	26.443654502306714	21.21701754768565	31.091382383321076	21.247945566686557
13	25.569739708999062	22.75945336708981	33.21272727633194	18.45807964757919
14	21.268365989488437	26.258086388301276	32.279533779939214	20.19401384227107
15	19.55344698719461	24.74281171378894	35.38125722397238	20.322484075044063
16	22.9115161271776	24.818347452502692	30.817986625614346	21.45214979470536
17	22.909930074921142	25.47239575175903	29.494822530665328	22.122851642654496
18	23.48408099175848	23.95375071620172	31.442494701594182	21.119673590445622
19	22.977139039288495	26.65043606524226	28.753739613835926	21.618685281633315
20	23.021945015533397	26.81459247378553	30.50652561375266	19.65693689692841
21	23.00430018418032	27.539814868050367	30.798755742004808	18.65712920576451
22	20.946992151023895	24.025123067742275	31.785676758585	23.24220802264883
23	21.003098749596052	26.08005202251401	31.25335797001172	21.66349125787822
24	21.922017775680665	25.591746184057403	30.099306696907398	22.386929343354538
25	21.536210564297566	27.885772516489986	28.134386207689577	22.443630711522868
26	19.52073465940519	25.965261490452956	30.830080274069832	23.683923576072022
27	22.46048251674772	25.532665737504384	29.819764986706897	22.187086759040994
28	19.514786963443477	26.355430345541308	31.12349994151432	23.00628274950089
29	21.398620531049946	24.930957162711117	30.848716388083197	22.82170591815574
30	23.58479531004348	25.54178553797901	30.07392986080409	20.79948929117342
31	23.03225435520037	26.20971179447935	28.606633267049563	22.151400583270718
32	24.350858549912076	25.597297366954997	27.776533167326527	22.275310915806397
33	24.874652307606905	24.364339994091956	27.055275903702835	23.705731794598304
34	21.760636958586193	24.558234882443788	31.13856743795066	22.542560721019356
35	23.704145742341844	24.02413178508199	28.918292535443314	23.35342993713285
36	23.868896920481287	26.480926730333447	28.16075432645317	21.489422022732093
37	22.24596894906195	26.346310545066682	28.626458920255278	22.781261585616093
38	22.667858849279437	26.852657727940493	26.413916022498153	24.06556740028192
39	22.45830169489509	23.774923324286227	28.772970497445467	24.993804483373218
40	24.32805904872551	22.697597329088	30.26722997955975	22.70711364262674
41	21.3720541557543	23.907160431168307	29.491650426152415	25.229134986924983
42	22.27094927210114	24.383372621169435	29.750375200486918	23.595302906242505
43	21.5707072008755	24.172031157996578	28.785262402433005	25.471999238694913
44	21.74596597521397	24.231706374145762	28.634587438069616	25.38774021257065
45	21.8405343410052	23.051485238809903	28.782090297920092	26.325890122264806
46	24.06556740028192	24.145663039232986	28.221817338326755	23.56695222215834
47	20.900401865990478	24.03900102498627	31.645311133888583	23.415285975134665
48	20.827245205661413	24.738450070083683	29.93475377530001	24.49955094895489
49	22.193629224598876	22.987646635487522	31.165926839374542	23.65279730053906
50	21.14108529590779	22.767780141436212	30.87508450684679	25.216050055809212
51	20.711265134408016	22.709492721011426	29.07967335253778	27.499568792042776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	450.0
2	732.0
3	3158.0
4	5584.0
5	3647.5
6	1711.0
7	1676.5
8	1642.0
9	1594.0
10	1546.0
11	1536.0
12	1526.0
13	1514.5
14	1503.0
15	1496.0
16	1489.0
17	1457.5
18	1426.0
19	1359.5
20	1293.0
21	1432.0
22	1571.0
23	1787.5
24	2004.0
25	2319.5
26	3115.5
27	3596.0
28	4646.5
29	5697.0
30	6682.0
31	7667.0
32	8770.5
33	9874.0
34	11139.0
35	12404.0
36	13930.0
37	15456.0
38	16847.0
39	18238.0
40	19531.5
41	20825.0
42	23054.0
43	25283.0
44	27626.5
45	29970.0
46	32873.0
47	35776.0
48	45514.0
49	55252.0
50	53219.5
51	51187.0
52	49620.5
53	48054.0
54	41387.5
55	34721.0
56	30931.5
57	27142.0
58	24909.5
59	22677.0
60	19705.5
61	16734.0
62	14545.5
63	12357.0
64	10888.0
65	9419.0
66	7766.5
67	6114.0
68	5126.5
69	4139.0
70	3368.5
71	2598.0
72	2368.0
73	2138.0
74	1631.0
75	855.0
76	586.0
77	440.5
78	295.0
79	222.0
80	149.0
81	92.5
82	36.0
83	37.0
84	38.0
85	24.5
86	11.0
87	9.0
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.08926376688696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1795208317724	28.996228558944363
2	9.550306955681732	7.466289352907568
3	3.573097600669728	4.190092637322297
4	2.0583368264528783	3.218354845212543
5	1.461312050197819	2.856080608795645
6	1.1496014211937622	2.6962243907898644
7	0.9140857444394282	2.5011657138960817
8	0.7812446886356861	2.443062376044784
9	0.7037820747562376	2.4759290839098167
>10	5.55283906020391	36.36907627736303
>50	0.045626178335667705	1.2173847580674027
>100	0.024607377079904885	1.929179105500044
>500	0.0030759221349881106	0.8875386162106258
>1k	0.0025632684458234255	2.7533936750358996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	3515	0.6968717101806712	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3485	0.6909240142189584	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	2705	0.5362839192144283	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	2343	0.4645150546097617	No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1692	0.3354500522405962	TruSeq Adapter, Index 22 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	905	0.1794221615116664	No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	788	0.15622614726098688	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	770	0.15265752968395926	Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	769	0.15245927315190216	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT	636	0.12609115438830923	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT	561	0.11122191448402746	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15860522564567195	0.0
2	0.0	0.0	0.0	0.7848976104140191	0.0
3	0.0	0.0	0.0	1.1427506507770664	0.0
4	0.0	0.0	0.0	1.7307795248583953	0.0
5	0.0	0.0	0.0	3.4962539428267814	0.0
6	0.0	0.0	0.0	4.515490774132281	0.0
7	0.0	0.0	0.0	5.374536327535652	0.0
8	0.0	0.0	0.0	6.569230189711676	0.0
9	0.0	0.0	0.0	7.02740103529561	0.0
10	0.0	0.0	0.0	8.450089909337288	0.0
11	0.0	0.0	0.0	9.797044788133157	0.0
12	0.0	0.0	0.0	11.386665662166905	0.0
13	0.0	0.0	0.0	11.935836255965043	0.0
14	0.0	0.0	0.0	12.193371491107204	0.0
15	0.0	0.0	0.0	12.62418293526726	0.0
16	0.0	0.0	0.0	13.327597111005815	0.0
17	0.0	0.0	0.0	14.122407548022688	0.0
18	0.0	0.0	0.0	14.991564184560971	0.0
19	0.0	0.0	0.0	15.611710616835548	0.0
20	0.0	0.0	0.0	16.153149205883462	0.0
21	0.0	0.0	0.0	16.81334345763357	0.0
22	0.0	0.0	0.0	17.517550659500355	0.0
23	0.0	0.0	0.0	18.18131352882749	0.0
24	0.0	0.0	0.0	18.744758592933742	0.0
25	0.0	0.0	0.0	19.254079623788403	0.0
26	1.9825653205708996E-4	0.0	0.0	19.742583718777073	0.0
27	1.9825653205708996E-4	0.0	0.0	20.248732645118825	0.0
28	1.9825653205708996E-4	0.0	0.0	20.753493775736175	0.0
29	1.9825653205708996E-4	0.0	0.0	21.271538094001354	0.0
30	1.9825653205708996E-4	0.0	0.0	21.923405571405063	0.0
31	1.9825653205708996E-4	0.0	0.0	22.487048892043372	0.0
32	1.9825653205708996E-4	0.0	0.0	23.023729324321913	0.0
33	1.9825653205708996E-4	0.0	0.0	23.564573143773654	0.0
34	1.9825653205708996E-4	0.0	0.0	24.11949317700145	0.0
35	1.9825653205708996E-4	0.0	0.0	24.696221428755525	0.0
36	1.9825653205708996E-4	0.0	0.0	25.197413941795848	0.0
37	1.9825653205708996E-4	0.0	0.0	25.72180246908685	0.0
38	1.9825653205708996E-4	0.0	0.0	26.302099338417953	0.0
39	1.9825653205708996E-4	0.0	0.0	27.063404421517177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACG	25	3.887681E-5	45.000004	1
CGCTCGA	20	7.029517E-4	45.0	41
GGCGATA	70	0.0	45.0	8
CCGCTCG	30	2.1630694E-6	44.999996	40
CGTTTTT	1650	0.0	42.81818	1
GCTACGA	70	0.0	41.785713	10
CTCACGA	60	3.6379788E-12	41.249996	24
CACGACG	55	6.002665E-11	40.909092	26
CCGATGA	325	0.0	40.846153	18
CGATGAA	335	0.0	39.62686	19
TTCGGGT	35	6.24132E-6	38.571426	4
TCACGAC	65	9.094947E-12	38.076927	25
CGAATAT	65	9.094947E-12	38.076927	14
TAACGGG	95	0.0	37.894737	3
AACGGGA	95	0.0	37.894737	4
TTACGGG	155	0.0	37.741936	3
GCGCGAC	60	1.546141E-10	37.499996	9
TGACCGG	30	1.1390138E-4	37.499996	25
CCCCGAT	30	1.1390138E-4	37.499996	40
CGGTCTA	60	1.546141E-10	37.499996	31
>>END_MODULE