SRR2935463.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935463.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301815
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1439	0.4767821347514206	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1103	0.3654556599241257	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	872	0.2889187084803605	Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	768	0.2544605138909597	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC	755	0.2501532395672846	No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	694	0.2299421831254245	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG	622	0.20608650994814706	No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	433	0.14346536785779368	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	314	0.10403724135646009	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGT	30	2.1601645E-6	45.000004	4
CAGGGCG	60	0.0	45.000004	5
ACAACGA	35	1.2079545E-7	45.0	13
GAATCGG	20	7.025012E-4	45.0	2
TCTAGCG	20	7.025012E-4	45.0	1
AACGAGC	35	1.2079545E-7	45.0	15
ACTGTCG	45	3.8198777E-10	45.0	26
CGCCACT	40	6.7866495E-9	45.0	22
ACCGCTT	25	3.883949E-5	44.999996	45
ACGGGTA	25	3.883949E-5	44.999996	5
AGCACGC	25	3.883949E-5	44.999996	37
AACGGGA	65	0.0	41.53846	4
TCGATCA	60	3.6379788E-12	41.250004	17
CCTCGAT	60	3.6379788E-12	41.250004	15
CTCGATC	60	3.6379788E-12	41.250004	16
ATTGCGG	60	3.6379788E-12	41.250004	2
CCGCTTG	45	1.9201252E-8	40.0	40
CATACGA	45	1.9201252E-8	40.0	18
ACATACG	45	1.9201252E-8	40.0	17
AGGGAAT	300	0.0	39.750004	6