##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935462.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 367811 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.196655347447468 31.0 31.0 33.0 28.0 34.0 2 31.21689400262635 31.0 31.0 34.0 28.0 34.0 3 31.0051792904508 31.0 31.0 34.0 27.0 34.0 4 34.82306945686779 35.0 35.0 37.0 32.0 37.0 5 34.85042589808353 35.0 35.0 37.0 33.0 37.0 6 35.01392563028294 36.0 35.0 37.0 32.0 37.0 7 35.397603660575676 37.0 35.0 37.0 33.0 37.0 8 35.14852736867576 37.0 35.0 37.0 32.0 37.0 9 36.996435669406296 39.0 37.0 39.0 33.0 39.0 10 36.408783315344024 38.0 35.0 39.0 32.0 39.0 11 36.62523415558534 39.0 35.0 39.0 32.0 39.0 12 36.689731954726746 39.0 35.0 39.0 33.0 39.0 13 36.681659874228885 39.0 35.0 39.0 33.0 39.0 14 37.631171987787205 40.0 36.0 41.0 33.0 41.0 15 37.83504027883886 40.0 37.0 41.0 33.0 41.0 16 37.84720957230752 40.0 37.0 41.0 33.0 41.0 17 37.724306777121946 39.0 36.0 41.0 33.0 41.0 18 37.661924194763074 39.0 36.0 41.0 33.0 41.0 19 37.486948459942745 39.0 36.0 40.0 33.0 41.0 20 37.451900568498495 39.0 35.0 41.0 33.0 41.0 21 37.414813586325586 39.0 35.0 41.0 33.0 41.0 22 37.536030733175465 39.0 35.0 41.0 33.0 41.0 23 37.56690528559505 39.0 35.0 41.0 33.0 41.0 24 37.56109795519982 39.0 35.0 41.0 33.0 41.0 25 37.159826650100186 39.0 35.0 41.0 32.0 41.0 26 37.22654026116674 39.0 35.0 41.0 32.0 41.0 27 37.25119694625772 39.0 35.0 41.0 33.0 41.0 28 37.108822194007246 39.0 35.0 41.0 32.0 41.0 29 37.057534983999936 39.0 35.0 41.0 32.0 41.0 30 36.7591915413079 39.0 35.0 41.0 31.0 41.0 31 36.67788619698704 39.0 35.0 41.0 31.0 41.0 32 36.55561959810881 39.0 35.0 41.0 31.0 41.0 33 36.47811240011854 39.0 35.0 41.0 30.0 41.0 34 36.286666249785895 39.0 35.0 41.0 30.0 41.0 35 36.242086288882064 39.0 35.0 41.0 30.0 41.0 36 36.04022718189505 39.0 35.0 41.0 29.0 41.0 37 36.04484640209238 39.0 35.0 41.0 29.0 41.0 38 35.84219069032737 39.0 35.0 41.0 29.0 41.0 39 35.775748958024636 39.0 35.0 41.0 28.0 41.0 40 35.65560573229185 38.0 35.0 40.0 27.0 41.0 41 35.49402546416502 38.0 35.0 40.0 26.0 41.0 42 35.50488430199206 38.0 35.0 40.0 27.0 41.0 43 35.495281544053874 38.0 35.0 40.0 27.0 41.0 44 35.49142358439525 38.0 35.0 40.0 27.0 41.0 45 35.471628091601396 38.0 35.0 40.0 27.0 41.0 46 35.348980862453814 38.0 35.0 40.0 27.0 41.0 47 35.29245998624293 38.0 35.0 40.0 27.0 41.0 48 35.20939014874487 38.0 34.0 40.0 26.0 41.0 49 35.17633784742708 38.0 34.0 40.0 27.0 41.0 50 35.03965895527866 37.0 34.0 40.0 26.0 41.0 51 33.914023778516686 36.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 10.0 9 23.0 10 28.0 11 22.0 12 20.0 13 25.0 14 35.0 15 37.0 16 61.0 17 100.0 18 169.0 19 326.0 20 624.0 21 1071.0 22 1612.0 23 2265.0 24 3001.0 25 3921.0 26 4861.0 27 5206.0 28 5485.0 29 6004.0 30 6852.0 31 8444.0 32 11347.0 33 15984.0 34 27511.0 35 28748.0 36 30403.0 37 44941.0 38 75398.0 39 83264.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.58421852527521 21.46102210102471 24.17328464890936 15.78147472479072 2 35.974182392587494 25.170264075843303 21.892221820445826 16.963331711123374 3 30.824798605805697 24.37094051020769 28.40127130509963 16.402989578886984 4 27.668286157836498 23.53409767516469 29.8144971194445 18.983119047554315 5 25.906783647036114 27.81999450804897 26.872497016130563 19.400724828784348 6 24.46963250147494 33.169481065003495 27.641370160218155 14.719516273303409 7 74.91238706835847 5.735010644053603 14.767366935736018 4.585235351851902 8 75.4719135643034 6.993265563020139 12.484400955925734 5.050419916750722 9 68.99331450119762 7.21457487677095 14.86741832082238 8.924692301209044 10 40.40227181895049 23.432415017495398 21.70027541318775 14.465037750366358 11 30.8449176343285 23.499569072159343 27.551106410629373 18.104406882882785 12 27.56062216736313 20.298740385687214 31.508573696817116 20.63206375013254 13 26.30481415727099 21.224215697736064 33.66946611167148 18.80150403332146 14 20.12365046178608 26.428790873573654 32.46096500648431 20.986593658155954 15 19.33493016793951 24.035985873179435 36.175100799051684 20.45398315982937 16 23.822832922343267 23.75459135262404 31.478123275269095 20.944452449763602 17 23.113229348768797 24.709157692401803 30.577117051964187 21.60049590686521 18 24.566693220159266 22.871529127731364 31.116524519386314 21.445253132723057 19 23.9111935205853 25.549806830138305 29.108156091035887 21.430843558240507 20 25.334478849191566 25.950828006775218 29.29466492301753 19.420028221015684 21 24.08573968695879 26.960041978081133 30.517303723923426 18.43691461103665 22 22.550984065185652 22.79322804375073 32.262221630130696 22.393566260932925 23 22.45936092177776 24.62677842696385 31.72390167776385 21.189958973494537 24 22.358222021636113 24.67707599827085 30.88597132766557 22.07873065242747 25 22.887026217269195 26.20503465094845 28.899896957948513 22.008042173833843 26 21.21986563751492 24.44461965520335 30.13558593951785 24.19992876776388 27 22.69535168877494 24.24805130896031 30.09045406472346 22.966142937541292 28 20.556481453790127 25.4165862358657 30.78537618505156 23.241556125292608 29 21.903640728526337 24.180081618004902 30.138848484683713 23.777429168785055 30 24.332605604508835 24.1009648977328 29.764471426901316 21.801958070857044 31 24.230107310548078 23.35764835744445 29.307443224917147 23.104801107090324 32 24.977230153529938 24.217872766176107 28.19165277819315 22.613244302100806 33 26.14059938392272 23.15237989075911 27.53234677592568 23.17467394939249 34 22.1306594963174 23.90303715767065 29.540715204276108 24.425588141735837 35 22.73531786705672 25.59983252268149 28.226181381198494 23.438668229063296 36 22.943305121380277 26.56853655817798 28.211228049188307 22.27693027125344 37 22.360668930510506 25.615329612219316 28.987170041135258 23.036831416134916 38 22.685292174513542 26.12673356696782 27.895576804391386 23.292397454127254 39 23.37776738596725 23.528116342360615 27.765074997757 25.329041273915138 40 24.411450446017113 23.620827000823795 29.36480964408351 22.602912909075584 41 20.936839844376596 24.926660703459113 28.867543385053736 25.268956067110555 42 21.280766480610964 25.044656086957705 29.907479656671498 23.767097775759833 43 22.089333924216515 24.08057399044618 29.80878766540424 24.021304419933063 44 22.662726237116345 23.094741592828928 29.05948979231181 25.183042377742915 45 22.136368950357657 23.006380994586895 28.284635315420147 26.572614739635302 46 22.801384406665377 24.367406086278006 28.06876357694577 24.762445930110847 47 20.83977912569227 24.299980152850242 31.59774993135061 23.262490790106877 48 21.2032810329218 24.73498617496486 30.261737685931088 23.79999510618225 49 22.387041170601204 23.091207168899246 30.898749629565188 23.623002030934366 50 20.78322834281737 23.044444021521922 31.16192827294453 25.01039936271618 51 20.768546889571002 22.86609155245493 29.305811952334217 27.059549605639855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 212.0 1 456.5 2 701.0 3 3902.0 4 7103.0 5 4441.5 6 1780.0 7 1699.0 8 1618.0 9 1609.5 10 1601.0 11 1565.0 12 1529.0 13 1483.0 14 1437.0 15 1415.5 16 1394.0 17 1318.0 18 1242.0 19 1255.5 20 1269.0 21 1462.5 22 1656.0 23 1578.5 24 1501.0 25 1885.0 26 2344.5 27 2420.0 28 3118.5 29 3817.0 30 4667.5 31 5518.0 32 6244.0 33 6970.0 34 8052.5 35 9135.0 36 9738.5 37 10342.0 38 11072.0 39 11802.0 40 12891.5 41 13981.0 42 15487.0 43 16993.0 44 17840.5 45 18688.0 46 20211.5 47 21735.0 48 27819.0 49 33903.0 50 32739.0 51 31575.0 52 31500.0 53 31425.0 54 28101.5 55 24778.0 56 23069.5 57 21361.0 58 19965.5 59 18570.0 60 17583.0 61 16596.0 62 14987.5 63 13379.0 64 11510.5 65 9642.0 66 8195.5 67 6749.0 68 5722.0 69 4695.0 70 3953.0 71 3211.0 72 2612.5 73 2014.0 74 1712.0 75 1154.0 76 898.0 77 698.5 78 499.0 79 359.5 80 220.0 81 145.0 82 70.0 83 57.5 84 45.0 85 34.5 86 24.0 87 19.0 88 14.0 89 14.5 90 15.0 91 9.5 92 4.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 367811.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.69386042601963 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69800247278494 29.253582238311747 2 8.595644375312494 6.823886162107097 3 3.561704700596006 4.241334277924676 4 2.2579722085183667 3.5851053476303734 5 1.7229977198474102 3.4196215502986593 6 1.4208534529688435 3.3839495188784 7 1.1664729229964017 3.241126937730522 8 1.0500066523734208 3.3343054004562145 9 0.9142671514683312 3.266171343223064 >10 5.552016778956936 33.08790233604473 >50 0.033137414718288606 0.9144436123701765 >100 0.02140124700556139 1.9678212707312668 >500 0.0027614512265240502 0.7683847435079337 >1k 0.0027614512265240502 2.712365260785138 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4734 1.287074067931628 No Hit GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC 2187 0.5945988564779194 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC 1516 0.41216820595360115 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG 1461 0.3972148739434111 No Hit GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC 844 0.22946567666546133 No Hit CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG 844 0.22946567666546133 No Hit CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT 559 0.1519802289762949 No Hit CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC 557 0.15143647144865163 No Hit GAATGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT 497 0.13512374561935342 No Hit CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC 494 0.1343081093278885 No Hit GAACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT 477 0.12968617034292068 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 415 0.1128296869859792 No Hit CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT 400 0.10875150552865466 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT 378 0.10277017272457865 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.7187876382163667E-4 0.0 0.0 0.08373865925706409 0.0 2 2.7187876382163667E-4 0.0 0.0 0.5565358295428903 0.0 3 2.7187876382163667E-4 0.0 0.0 0.9521194309033716 0.0 4 2.7187876382163667E-4 0.0 0.0 1.4692328396921246 0.0 5 2.7187876382163667E-4 0.0 0.0 2.9574971928517635 0.0 6 2.7187876382163667E-4 0.0 0.0 3.9879177077357664 0.0 7 2.7187876382163667E-4 0.0 0.0 4.7434687923960945 0.0 8 2.7187876382163667E-4 0.0 0.0 5.748332703480864 0.0 9 2.7187876382163667E-4 0.0 0.0 6.221945510058155 0.0 10 2.7187876382163667E-4 0.0 0.0 7.390208558199728 0.0 11 2.7187876382163667E-4 0.0 0.0 8.43041670858131 0.0 12 2.7187876382163667E-4 0.0 0.0 9.630489572090013 0.0 13 5.437575276432733E-4 0.0 0.0 10.040482747933043 0.0 14 5.437575276432733E-4 0.0 0.0 10.26287957673914 0.0 15 5.437575276432733E-4 0.0 0.0 10.583696518048672 0.0 16 5.437575276432733E-4 0.0 0.0 11.07335017169144 0.0 17 5.437575276432733E-4 0.0 0.0 11.609495093947707 0.0 18 5.437575276432733E-4 0.0 0.0 12.196753223802443 0.0 19 5.437575276432733E-4 0.0 0.0 12.630671730861774 0.0 20 5.437575276432733E-4 0.0 0.0 13.006136303699455 0.0 21 5.437575276432733E-4 0.0 0.0 13.429995296497387 0.0 22 5.437575276432733E-4 0.0 0.0 13.91584264744665 0.0 23 5.437575276432733E-4 0.0 0.0 14.354926851018593 0.0 24 5.437575276432733E-4 0.0 0.0 14.7080973652229 0.0 25 5.437575276432733E-4 0.0 0.0 15.055558425386952 0.0 26 5.437575276432733E-4 0.0 0.0 15.381269184445273 0.0 27 5.437575276432733E-4 0.0 0.0 15.754286848408558 0.0 28 5.437575276432733E-4 0.0 0.0 16.10337918115554 0.0 29 5.437575276432733E-4 0.0 0.0 16.484281329269653 0.0 30 5.437575276432733E-4 0.0 0.0 16.96414734741484 0.0 31 5.437575276432733E-4 0.0 0.0 17.345865131820418 0.0 32 0.0010875150552865467 0.0 0.0 17.737642430487398 0.0 33 0.0010875150552865467 0.0 0.0 18.102231852772213 0.0 34 0.0010875150552865467 0.0 0.0 18.51739072512785 0.0 35 0.0010875150552865467 0.0 0.0 18.93798717275992 0.0 36 0.0010875150552865467 0.0 0.0 19.303664110100023 0.0 37 0.0013593938191081834 0.0 0.0 19.69680080258611 0.0 38 0.0013593938191081834 0.0 0.0 20.142410096489773 0.0 39 0.0013593938191081834 0.0 0.0 20.77643137372183 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGATA 25 3.8856153E-5 45.000004 9 GACGGGT 35 1.2089185E-7 45.000004 4 GCGTAAG 25 3.8856153E-5 45.000004 1 GATAGGC 25 3.8856153E-5 45.000004 3 TCAGTCG 25 3.8856153E-5 45.000004 15 AACACGT 30 2.1614615E-6 45.000004 41 CGAATAT 45 3.8380676E-10 45.0 14 GTTCACG 20 7.027025E-4 45.0 1 TCGACGG 20 7.027025E-4 45.0 2 CCGATGA 330 0.0 43.636364 18 CGTTTTT 1885 0.0 43.448273 1 CGATGAA 335 0.0 42.985077 19 GCGATGT 60 3.6379788E-12 41.250004 9 CGTTAGG 55 6.002665E-11 40.909092 2 CACAACG 105 0.0 40.714287 12 CTACGAA 50 1.0768417E-9 40.500004 11 TATGCGG 45 1.9221261E-8 40.0 2 AATGGGC 85 0.0 39.705883 4 GCGATTG 40 3.449677E-7 39.375 1 TGTAGCG 40 3.449677E-7 39.375 1 >>END_MODULE