##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935460.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538999
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167881573064143	31.0	31.0	33.0	28.0	34.0
2	31.256237952203993	31.0	31.0	34.0	28.0	34.0
3	30.987866396783666	31.0	31.0	34.0	27.0	34.0
4	34.731172042990806	35.0	35.0	37.0	32.0	37.0
5	34.81371579539108	35.0	35.0	37.0	33.0	37.0
6	35.03375330937534	37.0	35.0	37.0	32.0	37.0
7	35.32037350718647	37.0	35.0	37.0	33.0	37.0
8	35.140911207627475	37.0	35.0	37.0	32.0	37.0
9	37.0707997602964	39.0	37.0	39.0	33.0	39.0
10	36.51244436446079	38.0	35.0	39.0	32.0	39.0
11	36.83378447826434	39.0	37.0	39.0	33.0	39.0
12	36.9895250269481	39.0	37.0	39.0	33.0	39.0
13	37.07561424047169	39.0	37.0	39.0	33.0	39.0
14	38.18333800248238	40.0	38.0	41.0	33.0	41.0
15	38.34157948344988	40.0	38.0	41.0	34.0	41.0
16	38.27280941152024	40.0	38.0	41.0	34.0	41.0
17	38.15827858678773	40.0	37.0	41.0	33.0	41.0
18	38.007816341032175	39.0	37.0	41.0	34.0	41.0
19	37.757153538318256	39.0	37.0	41.0	34.0	41.0
20	37.69305508915601	39.0	35.0	41.0	34.0	41.0
21	37.635667227582985	39.0	35.0	41.0	33.0	41.0
22	37.73929821762192	39.0	35.0	41.0	33.0	41.0
23	37.78193280507014	39.0	35.0	41.0	34.0	41.0
24	37.753808448624206	40.0	35.0	41.0	33.0	41.0
25	37.3637260922562	39.0	35.0	41.0	33.0	41.0
26	37.42079484377522	39.0	35.0	41.0	33.0	41.0
27	37.49535342366127	39.0	35.0	41.0	33.0	41.0
28	37.372535013979615	39.0	35.0	41.0	33.0	41.0
29	37.29500240260186	39.0	35.0	41.0	33.0	41.0
30	36.979419256807525	39.0	35.0	41.0	32.0	41.0
31	36.86198675693276	39.0	35.0	41.0	31.0	41.0
32	36.664027205987395	39.0	35.0	41.0	31.0	41.0
33	36.421889465472105	39.0	35.0	41.0	30.0	41.0
34	36.13773865999752	39.0	35.0	41.0	29.0	41.0
35	36.02243603420415	39.0	35.0	41.0	27.0	41.0
36	35.83531323805796	39.0	35.0	41.0	26.0	41.0
37	35.8174542067796	39.0	35.0	41.0	26.0	41.0
38	35.63278967122388	39.0	35.0	41.0	25.0	41.0
39	35.53521249575602	39.0	35.0	41.0	24.0	41.0
40	35.43580043747762	39.0	35.0	41.0	24.0	41.0
41	35.25716745300084	39.0	35.0	41.0	23.0	41.0
42	35.27416006337674	39.0	35.0	41.0	23.0	41.0
43	35.23659413097241	39.0	35.0	41.0	23.0	41.0
44	35.20381299408719	39.0	35.0	41.0	23.0	41.0
45	35.18678142260004	39.0	35.0	41.0	23.0	41.0
46	35.07898530423989	39.0	35.0	40.0	23.0	41.0
47	35.028881315178694	38.0	35.0	40.0	23.0	41.0
48	34.93719468867289	38.0	35.0	40.0	23.0	41.0
49	34.91785513516723	38.0	35.0	40.0	23.0	41.0
50	34.804517262555215	38.0	34.0	40.0	22.0	41.0
51	33.721340855919955	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	16.0
9	33.0
10	28.0
11	22.0
12	17.0
13	17.0
14	21.0
15	44.0
16	69.0
17	120.0
18	243.0
19	415.0
20	778.0
21	1298.0
22	2022.0
23	3059.0
24	4762.0
25	7117.0
26	9912.0
27	10384.0
28	9910.0
29	9411.0
30	9771.0
31	11385.0
32	13995.0
33	19215.0
34	31524.0
35	39277.0
36	42263.0
37	63625.0
38	113644.0
39	134581.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.201185902014664	18.82007202239707	21.401709465138154	20.577032610450114
2	41.16705225798192	21.97054168931668	21.128054040916588	15.734352011784807
3	29.533449969294935	21.723416926562017	33.115831383731695	15.627301720411355
4	25.661272098835063	23.08037677249865	33.48930146438119	17.76904966428509
5	23.393549895268823	27.225096892573085	31.19170907552704	18.18964413663105
6	22.216924335666672	31.999873840211208	31.754789897569385	14.028411926552739
7	70.95634685778637	5.399082373065627	18.815248265766726	4.829322503381268
8	71.42109725621012	5.741569093820211	17.763483791250074	5.07384985871959
9	65.03852511785736	6.9005693888114825	20.140482635403774	7.92042285792738
10	33.20043265386392	24.438635322143455	27.2555236651645	15.105408358828123
11	25.3147037378548	23.993179950241096	32.634383366202904	18.0577329457012
12	22.696888120386124	20.667941870022023	36.4538709719313	20.18129903766055
13	21.96720216549567	21.12972380282709	38.04478301443973	18.85829101723751
14	18.56329974638172	25.06145651476162	36.41453880248386	19.960704936372796
15	18.217659030907292	24.50041651283212	38.74125926022126	18.540665196039324
16	20.76219065341494	24.596891645439044	35.60674509600204	19.03417260514398
17	20.227124725648842	24.749211037497286	34.6989512039911	20.32471303286277
18	21.35477060254286	24.22750320501522	34.77817213018948	19.639554062252433
19	20.392431154788785	26.568509403542496	33.22640672802733	19.8126527136414
20	21.871283620192244	25.25793183289765	33.872419058291385	18.998365488618717
21	20.812283510730076	26.63010506512999	34.521585383275294	18.036026040864638
22	20.34029747736081	23.606908361611058	34.54199358440368	21.510800576624447
23	19.406529511186477	25.481679928905248	34.73846890253971	20.373321657368567
24	19.834545147579124	24.008207807435635	34.76685485501829	21.39039218996696
25	20.155139434395984	26.21656069862838	32.83067315523777	20.79762671173787
26	19.43101935254054	25.302644346278935	33.84922792064549	21.41710838053503
27	20.55365594370305	24.84457299549721	32.954606594817434	21.64716446598231
28	18.81116662554105	25.98446379306826	34.02974773608115	21.174621845309545
29	19.717105226540312	25.61748723095961	33.312863289171226	21.352544253328855
30	19.62879337438474	24.738264820528425	33.922326386505354	21.71061541858148
31	20.141595810010777	24.888357863372658	33.10043246833482	21.869613858281742
32	20.62879522967575	25.316744557967642	33.78855990456383	20.265900307792776
33	20.055510307069216	24.313032120653286	33.91731710077384	21.714140471503658
34	20.40764454108449	24.794294609080907	34.05219675732237	20.74586409251223
35	20.6720235102477	24.635110640279482	32.46722164605129	22.225644203421528
36	21.07425060157811	26.333629561464868	31.257571906441385	21.33454793051564
37	21.926756821441227	24.885203868652816	32.02825979268978	21.159779517216172
38	20.1866793815944	25.315074796057136	32.6445874667671	21.853658355581366
39	22.81562674513311	23.91841172247073	31.677424262382676	21.58853727001349
40	22.683158966899754	23.54438505451773	32.72455050937015	21.047905469212374
41	21.019148458531465	25.34141992842287	31.434752198056024	22.204679414989638
42	21.379445972998095	25.461642785979194	31.19263672103288	21.966274519989835
43	21.656255391939503	24.44735518989831	31.92269373412567	21.97369568403652
44	21.78315729713784	24.317113760878964	31.37686711849187	22.522861823491326
45	20.899482188278643	23.643828652743327	31.604325796522815	23.85236336245522
46	21.606348063725537	24.507095560474138	30.504509284803866	23.38204709099646
47	20.610984435963704	24.356260401225235	32.92455088042835	22.10820428238271
48	20.20040853508077	23.836778917957176	32.67853929228069	23.284273254681363
49	20.948832929189106	23.050135529008404	33.03865127764615	22.962380264156334
50	19.494470305139714	23.02601674585667	34.4481158592131	23.03139708979052
51	19.670166363945015	22.45774110898165	32.50339982077889	25.368692706294443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	460.5
2	676.0
3	9417.0
4	18158.0
5	11758.5
6	5359.0
7	5008.0
8	4657.0
9	4714.0
10	4771.0
11	4603.5
12	4436.0
13	4256.5
14	4077.0
15	3895.5
16	3714.0
17	3508.5
18	3303.0
19	3209.0
20	3115.0
21	3174.0
22	3233.0
23	3283.0
24	3333.0
25	3583.0
26	4489.5
27	5146.0
28	5794.5
29	6443.0
30	7509.5
31	8576.0
32	9737.0
33	10898.0
34	12151.5
35	13405.0
36	14754.5
37	16104.0
38	17193.0
39	18282.0
40	20137.5
41	21993.0
42	24050.0
43	26107.0
44	29228.0
45	32349.0
46	42084.5
47	51820.0
48	51756.5
49	51693.0
50	50419.5
51	49146.0
52	42897.0
53	36648.0
54	32538.5
55	28429.0
56	25566.0
57	22703.0
58	21126.0
59	19549.0
60	18290.5
61	17032.0
62	15182.5
63	13333.0
64	10977.0
65	8621.0
66	7479.5
67	6338.0
68	5420.5
69	4503.0
70	3735.5
71	2968.0
72	2376.5
73	1785.0
74	1407.0
75	840.0
76	651.0
77	470.5
78	290.0
79	209.5
80	129.0
81	101.5
82	74.0
83	48.5
84	23.0
85	15.0
86	7.0
87	4.5
88	2.0
89	4.0
90	6.0
91	4.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.57665605739498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.60727589727895	31.019210493603417
2	8.880392287744506	7.384340316045926
3	3.7577298903941423	4.687015296285294
4	2.27785718741911	3.788227393167578
5	1.6559781802310978	3.442501761900959
6	1.2676247115981973	3.162215798638366
7	1.0874055584389055	3.164748082867973
8	0.9154220616053597	3.044815056217398
9	0.8358207569606322	3.1275568924005355
>10	4.646557332356825	28.657062069058192
>50	0.03959192030267	1.1326287953690322
>100	0.021595592891155185	1.5417219704421892
>500	0.0022495409261619986	0.6726561763783198
>1k	0.004049173667091598	2.92313628149722
>5k	0.0	0.0
>10k+	4.4990818523239973E-4	2.2521636161276337
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12040	2.2337703780526494	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	2705	0.5018562186571774	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	2523	0.46808992224475365	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1947	0.36122515997246746	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1886	0.3499078848012705	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	1553	0.28812669411260505	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	1421	0.26363685275853943	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	1295	0.24026018601147683	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1153	0.21391505364573962	No Hit
CGTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1144	0.21224529173523513	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	799	0.14823775183256369	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	772	0.14322846610105028	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	713	0.13228224913218764	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	710	0.13172566182868614	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	602	0.11168851890263247	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.421164046686543E-4	0.0	0.0	0.13469412744736076	0.0
2	7.421164046686543E-4	0.0	0.0	0.5788507956415504	0.0
3	7.421164046686543E-4	0.0	0.0	1.1248629403765127	0.0
4	7.421164046686543E-4	0.0	0.0	1.7827491331152747	0.0
5	7.421164046686543E-4	0.0	0.0	3.4862773400321707	0.0
6	7.421164046686543E-4	0.0	0.0	5.191475308859571	0.0
7	7.421164046686543E-4	0.0	0.0	6.366245577450051	0.0
8	7.421164046686543E-4	0.0	0.0	7.859198254542216	0.0
9	7.421164046686543E-4	0.0	0.0	8.652335162031841	0.0
10	7.421164046686543E-4	0.0	0.0	10.017643817520998	0.0
11	7.421164046686543E-4	0.0	0.0	11.140651466885839	0.0
12	7.421164046686543E-4	0.0	0.0	12.30150705288878	0.0
13	7.421164046686543E-4	0.0	0.0	12.879615732125663	0.0
14	7.421164046686543E-4	0.0	0.0	13.184996632646813	0.0
15	7.421164046686543E-4	0.0	0.0	13.51486737452203	0.0
16	7.421164046686543E-4	0.0	0.0	14.012456423852363	0.0
17	7.421164046686543E-4	0.0	0.0	14.57590830409704	0.0
18	7.421164046686543E-4	0.0	0.0	15.211345475594575	0.0
19	7.421164046686543E-4	0.0	0.0	15.628971482321859	0.0
20	7.421164046686543E-4	0.0	0.0	16.061625346243684	0.0
21	7.421164046686543E-4	0.0	0.0	16.510234712865888	0.0
22	7.421164046686543E-4	0.0	0.0	17.006710587589215	0.0
23	7.421164046686543E-4	0.0	0.0	17.517657732203585	0.0
24	9.276455058358178E-4	0.0	0.0	17.94177725747172	0.0
25	9.276455058358178E-4	0.0	0.0	18.326379084191252	0.0
26	9.276455058358178E-4	0.0	0.0	18.683151545735708	0.0
27	9.276455058358178E-4	0.0	0.0	19.067011256050566	0.0
28	9.276455058358178E-4	0.0	0.0	19.449201204454926	0.0
29	9.276455058358178E-4	0.0	0.0	19.868868031295047	0.0
30	9.276455058358178E-4	0.0	0.0	20.393544329395787	0.0
31	9.276455058358178E-4	0.0	0.0	20.813582214438245	0.0
32	9.276455058358178E-4	0.0	0.0	21.25272959690092	0.0
33	9.276455058358178E-4	0.0	0.0	21.672953011044548	0.0
34	9.276455058358178E-4	0.0	0.0	22.09150666327767	0.0
35	9.276455058358178E-4	0.0	0.0	22.510987961016625	0.0
36	9.276455058358178E-4	0.0	0.0	22.931025846059082	0.0
37	9.276455058358178E-4	0.0	0.0	23.33232529188366	0.0
38	9.276455058358178E-4	0.0	0.0	23.769431854233495	0.0
39	0.0011131746070029815	0.0	0.0	24.22230839018254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	25	3.888039E-5	45.0	28
TAGCGCC	20	7.029949E-4	45.0	20
TCGTCCC	25	3.888039E-5	45.0	38
AATCGCG	20	7.029949E-4	45.0	1
TGCGTAG	20	7.029949E-4	45.0	1
CGTTTTT	5445	0.0	43.512394	1
TACGGGT	60	3.6379788E-12	41.250004	4
GCTAGCG	50	1.0786607E-9	40.5	1
CGTTTCT	300	0.0	39.75	1
ACACGAC	80	0.0	39.375	26
GCTACGA	40	3.4536606E-7	39.375	10
ACGGGTG	70	0.0	38.571426	5
TACGCGG	35	6.242117E-6	38.571426	2
GTTTTTT	6345	0.0	38.014183	2
CGTTCTG	250	0.0	37.8	1
CTCGTCC	30	1.1391174E-4	37.500004	37
ACCATAG	30	1.1391174E-4	37.500004	21
CCCGTCG	30	1.1391174E-4	37.500004	40
GGACGCA	30	1.1391174E-4	37.500004	8
CGGTCTA	30	1.1391174E-4	37.500004	31
>>END_MODULE