FastQCFastQC Report
Sat 14 Jan 2017
SRR2935459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673573
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191602.8445320700206214No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC108691.613633563103034No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC44570.6616951688978032No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG41990.6233919708776925No Hit
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT36960.5487155809392598TruSeq Adapter, Index 8 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT27990.4155451599158517No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC25940.38511044831072505TruSeq Adapter, Index 13 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC19700.292470155424876No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT17150.254612343428255No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG16900.25090079323250786TruSeq Adapter, Index 8 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT13650.2026506406877948No Hit
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11970.1777090233723739TruSeq Adapter, Index 8 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT10450.15514279818223117No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC10040.14905585586120584No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC7880.1169880621699504No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC7210.10704110764534801No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT7050.10466571552006983No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG7000.10392340548092041No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC6850.1016964753634721TruSeq Adapter, Index 13 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT502.1827873E-1145.04
TTACGAG207.031205E-445.01
TCGACAG207.031205E-445.01
CGTCATA253.88908E-545.038
TGCGTTG253.88908E-545.01
ATCGACG207.031205E-445.01
CTACGAA302.164159E-644.99999611
CGTTTTT79050.044.288431
CCGATGA7100.040.88028318
ACGGGCG451.9263098E-840.05
CGATGAA7200.040.019
TAGTGCG451.9263098E-840.01
TAGCCGT403.4553705E-739.37544
CGGCGAA1200.039.37499631
GTTTTTT90900.039.0346532
TCACGAC356.244438E-638.57142625
CACGACG356.244438E-638.57142626
GGTTACG356.244438E-638.5714261
CACGCCG1700.038.3823526
TAGTAGG1350.038.3333322
Produced by FastQC (version 0.11.5)