##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935459.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	673573
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0452957585889	31.0	30.0	33.0	28.0	34.0
2	31.12307945835121	31.0	31.0	34.0	28.0	34.0
3	30.86772183564365	31.0	30.0	34.0	27.0	34.0
4	34.62698029760694	35.0	35.0	37.0	32.0	37.0
5	34.687376127012215	35.0	35.0	37.0	32.0	37.0
6	34.863364475713844	35.0	35.0	37.0	32.0	37.0
7	35.12046504239333	36.0	35.0	37.0	32.0	37.0
8	34.94404912310915	37.0	35.0	37.0	32.0	37.0
9	36.85480118710221	39.0	37.0	39.0	32.0	39.0
10	36.36319597133495	38.0	35.0	39.0	32.0	39.0
11	36.651712583491324	39.0	35.0	39.0	32.0	39.0
12	36.74551681851856	39.0	35.0	39.0	33.0	39.0
13	36.73653041318462	39.0	35.0	39.0	33.0	39.0
14	37.72247106104312	40.0	37.0	41.0	33.0	41.0
15	37.87971162739599	40.0	37.0	41.0	33.0	41.0
16	37.93573079681044	40.0	37.0	41.0	33.0	41.0
17	37.763225366812506	40.0	36.0	41.0	33.0	41.0
18	37.60905351016148	39.0	36.0	40.0	33.0	41.0
19	37.3485873097645	39.0	36.0	40.0	33.0	41.0
20	37.114041091314526	39.0	35.0	40.0	32.0	41.0
21	37.130042326518435	39.0	35.0	40.0	32.0	41.0
22	37.23077379883101	39.0	35.0	40.0	33.0	41.0
23	37.28276222473288	39.0	35.0	41.0	33.0	41.0
24	37.23556466782368	39.0	35.0	41.0	33.0	41.0
25	36.88748806736612	39.0	35.0	40.0	32.0	41.0
26	36.867255664939066	39.0	35.0	40.0	32.0	41.0
27	36.90491156860504	39.0	35.0	40.0	32.0	41.0
28	36.738066994965656	39.0	35.0	40.0	32.0	41.0
29	36.65907184521945	39.0	35.0	40.0	31.0	41.0
30	36.31537784323303	38.0	35.0	40.0	31.0	41.0
31	36.15951797355298	38.0	35.0	40.0	30.0	41.0
32	35.87481386575768	38.0	35.0	40.0	29.0	41.0
33	35.541755682012194	38.0	35.0	40.0	26.0	41.0
34	35.26880679599687	38.0	35.0	40.0	24.0	41.0
35	35.12355156753611	38.0	35.0	40.0	23.0	41.0
36	34.92024769401387	38.0	35.0	40.0	22.0	41.0
37	34.92885255198768	38.0	35.0	40.0	22.0	41.0
38	34.753924222021965	38.0	34.0	40.0	21.0	41.0
39	34.60631290149694	38.0	34.0	40.0	20.0	41.0
40	34.46584854202885	38.0	34.0	40.0	20.0	41.0
41	34.34954340509492	38.0	34.0	40.0	19.0	41.0
42	34.375233270929805	38.0	34.0	40.0	18.0	41.0
43	34.375988942549654	38.0	34.0	40.0	18.0	41.0
44	34.348002369453646	38.0	34.0	40.0	18.0	41.0
45	34.338478531651354	38.0	34.0	40.0	20.0	41.0
46	34.20476473967929	38.0	34.0	40.0	20.0	41.0
47	34.11698212368964	37.0	34.0	40.0	20.0	41.0
48	34.06318691515248	37.0	34.0	40.0	20.0	41.0
49	34.051170400238725	37.0	34.0	40.0	19.0	41.0
50	33.94668729298829	37.0	34.0	40.0	18.0	41.0
51	32.8630660670781	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	28.0
10	29.0
11	20.0
12	15.0
13	27.0
14	34.0
15	58.0
16	120.0
17	214.0
18	363.0
19	715.0
20	1425.0
21	2474.0
22	3639.0
23	5536.0
24	7805.0
25	11320.0
26	14527.0
27	15227.0
28	14421.0
29	13580.0
30	14369.0
31	17204.0
32	21526.0
33	29806.0
34	47218.0
35	52957.0
36	54061.0
37	78741.0
38	131200.0
39	134889.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.867325442082745	17.218475206102383	19.108990413808154	21.80520893800672
2	41.100964557664874	24.397652518732194	19.746931661453175	14.75445126214976
3	28.37034738625212	24.227960443782635	33.21377192969433	14.187920240270913
4	24.252011289051076	20.926462313661624	37.530156345340444	17.291370051946856
5	24.853430882769945	24.97561511521394	32.628386232821086	17.542567769195024
6	21.8835968781409	31.53644816523228	33.18645492025363	13.393500036373194
7	66.88064990728547	5.048895962278773	23.26726279111544	4.803191339320311
8	66.45025854658664	7.399049546225873	20.504978673432575	5.6457132337549165
9	60.72541506265839	5.928830282686509	22.394751571099196	10.951003083555904
10	36.00708460701364	19.028524005564357	29.66404532248175	15.300346064940252
11	28.422457551000413	21.457807839684786	32.614133880069424	17.505600729245383
12	24.017441316679854	18.653954359809553	37.077050297443634	20.25155402606696
13	23.770994383682247	18.93350832055323	39.81246279170929	17.48303450405524
14	18.597538796834197	24.06331607709929	38.49055707399198	18.848588052074533
15	17.801040124826855	22.010977280858942	41.69332796890612	18.494654625408085
16	21.448603195199333	23.074410642944418	35.86040414327771	19.616582018578534
17	21.130152188404228	24.054408356629498	35.37211259952522	19.44332685544106
18	21.288264226743056	22.581516776949194	35.99639534244989	20.13382365385786
19	20.466230089388976	24.6674079869591	33.59784314395024	21.26851877970168
20	21.5949867349196	25.79809463859151	34.94780818114741	17.659110445341486
21	20.390662927403564	26.747509178663638	36.04924781723733	16.812580076695475
22	19.1559044082824	21.90675695136236	37.778681746447674	21.158656893907565
23	19.982540867879205	24.261809781567848	36.893105869742406	18.862543480810544
24	21.041223445714124	23.472882671959834	34.78969614280857	20.69619773951747
25	20.717576268644972	26.45236670709782	32.866816217395886	19.96324080686132
26	19.40056979718605	22.810148269007218	34.93756430260714	22.851717631199588
27	21.780861168722616	22.61670227280488	34.034024522954454	21.56841203551805
28	19.320103388942254	24.234492772127147	33.71438582009671	22.73101801883389
29	21.381349905652396	23.58096301365999	32.47398574467801	22.563701336009608
30	21.80342739391276	25.37141482808842	32.62319006254704	20.201967715451776
31	23.965182689923736	22.94094329790535	30.964720973079384	22.12915303909153
32	26.526003862981444	23.79370907088022	30.11922983848818	19.56105722765016
33	23.901344026556885	25.101510897853686	29.183919189159894	21.81322588642953
34	21.836831345674483	24.07207533556125	30.860946029606296	23.23014728915797
35	21.150491483476923	25.885093375179824	31.14079691436563	21.823618226977626
36	21.718952511457555	26.893744256376074	28.563644920446635	22.82365831171974
37	21.538422709936413	25.937500463943774	30.35706597503166	22.167010851088154
38	22.244804943191014	25.5029521670257	29.461543143801784	22.790699745981506
39	22.511442709253487	23.46545957156834	29.53443798964626	24.488659729531914
40	22.01142266688243	23.48743194872716	32.89710246699318	21.604042917397223
41	19.327526489333746	24.586496192691808	30.550511971234002	25.535465346740445
42	20.673928438342987	23.877441643296272	33.442997269783675	22.005632648577066
43	21.981433341300793	23.38009391706615	30.778846539276365	23.859626202356683
44	22.24643802527714	23.099055336244177	30.77335344498666	23.88115319349202
45	21.132082194506015	22.14830463810159	30.599949819841356	26.119663347551047
46	22.94213099396799	23.67018868036575	30.011446420803683	23.376233904862577
47	19.699720742963272	24.058119906825244	33.694046525024014	22.54811282518747
48	20.254671728231386	24.10547928732298	31.682831704952548	23.957017279493094
49	20.859357486122516	22.12974688712285	34.26191370497333	22.748981921781304
50	19.758511698063906	22.987263444348276	33.46155502076241	23.792669836825407
51	19.356773504876234	22.14533539794499	31.922300923582153	26.575590173596623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	279.0
1	561.0
2	843.0
3	14410.0
4	27977.0
5	17636.0
6	7295.0
7	7008.0
8	6721.0
9	6659.5
10	6598.0
11	6377.0
12	6156.0
13	5966.0
14	5776.0
15	5477.5
16	5179.0
17	4878.5
18	4578.0
19	4241.5
20	3905.0
21	3827.0
22	3749.0
23	3687.0
24	3625.0
25	3755.0
26	4541.5
27	5198.0
28	6005.5
29	6813.0
30	7683.5
31	8554.0
32	9754.5
33	10955.0
34	12491.0
35	14027.0
36	15142.0
37	16257.0
38	17270.5
39	18284.0
40	20193.5
41	22103.0
42	23710.5
43	25318.0
44	27538.0
45	29758.0
46	33159.0
47	36560.0
48	57267.5
49	77975.0
50	69727.5
51	61480.0
52	61147.5
53	60815.0
54	53767.0
55	46719.0
56	41198.0
57	35677.0
58	32939.5
59	30202.0
60	26500.5
61	22799.0
62	20543.5
63	18288.0
64	15754.0
65	13220.0
66	11230.0
67	9240.0
68	7569.0
69	5898.0
70	5118.0
71	4338.0
72	3542.5
73	2747.0
74	2263.0
75	1354.5
76	930.0
77	739.0
78	548.0
79	412.5
80	277.0
81	218.5
82	160.0
83	95.0
84	30.0
85	29.5
86	29.0
87	20.0
88	11.0
89	9.5
90	8.0
91	8.0
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	673573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.66609690580468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27860074646705	29.799546790776
2	9.273716696117155	7.5425172368255815
3	4.064548386717974	4.958679557178156
4	2.547510890581229	4.14389298979876
5	1.917014776883575	3.8978754343303494
6	1.5018889183418715	3.664557617702683
7	1.2713792504234773	3.6191422261225306
8	1.0726797815414637	3.4897359956050042
9	0.8862703096138285	3.243703886594411
>10	4.117323418898558	23.608842407964513
>50	0.039888861995705786	1.0584361141657168
>100	0.02031377130370483	1.316924573709467
>500	0.0036934129643099427	0.9807843825641859
>1k	0.004432095557171931	4.165104397073114
>5k	0.0	0.0
>10k+	7.386825928619885E-4	4.510256389589577
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19160	2.8445320700206214	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	10869	1.613633563103034	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	4457	0.6616951688978032	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	4199	0.6233919708776925	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	3696	0.5487155809392598	TruSeq Adapter, Index 8 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT	2799	0.4155451599158517	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2594	0.38511044831072505	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1970	0.292470155424876	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	1715	0.254612343428255	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	1690	0.25090079323250786	TruSeq Adapter, Index 8 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT	1365	0.2026506406877948	No Hit
CGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	1197	0.1777090233723739	TruSeq Adapter, Index 8 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	1045	0.15514279818223117	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	1004	0.14905585586120584	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	788	0.1169880621699504	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC	721	0.10704110764534801	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT	705	0.10466571552006983	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	700	0.10392340548092041	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	685	0.1016964753634721	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09397645095631803	0.0
2	0.0	0.0	0.0	0.6386835576841708	0.0
3	0.0	0.0	0.0	1.0831788091268504	0.0
4	0.0	0.0	0.0	2.083961203908114	0.0
5	0.0	0.0	0.0	4.749002706462402	0.0
6	0.0	0.0	0.0	6.452307322294688	0.0
7	0.0	0.0	0.0	7.760851459307306	0.0
8	0.0	0.0	0.0	9.376118104496468	0.0
9	0.0	0.0	0.0	10.36888355085492	0.0
10	0.0	0.0	0.0	12.243364861715063	0.0
11	1.484620078298863E-4	0.0	0.0	13.336193701350856	0.0
12	1.484620078298863E-4	0.0	0.0	14.859710825701148	0.0
13	1.484620078298863E-4	0.0	0.0	15.430101859783573	0.0
14	1.484620078298863E-4	0.0	0.0	15.827386192736348	0.0
15	1.484620078298863E-4	0.0	0.0	16.20551892667907	0.0
16	1.484620078298863E-4	0.0	0.0	16.59285630510724	0.0
17	1.484620078298863E-4	0.0	0.0	16.976779057355326	0.0
18	1.484620078298863E-4	0.0	0.0	17.41444505643783	0.0
19	1.484620078298863E-4	0.0	0.0	17.831474836431983	0.0
20	2.969240156597726E-4	0.0	0.0	18.152598159368026	0.0
21	2.969240156597726E-4	0.0	0.0	18.484113822852162	0.0
22	2.969240156597726E-4	0.0	0.0	18.877538143601363	0.0
23	4.453860234896589E-4	0.0	0.0	19.225830013970274	0.0
24	4.453860234896589E-4	0.0	0.0	19.53210713612333	0.0
25	4.453860234896589E-4	0.0	0.0	19.81715419115671	0.0
26	4.453860234896589E-4	0.0	0.0	20.098638158002178	0.0
27	5.938480313195452E-4	0.0	0.0	20.439803851995254	0.0
28	5.938480313195452E-4	0.0	0.0	20.74697174619529	0.0
29	5.938480313195452E-4	0.0	0.0	21.081456649836024	0.0
30	5.938480313195452E-4	0.0	0.0	21.5820705402384	0.0
31	5.938480313195452E-4	0.0	0.0	21.939863979108427	0.0
32	5.938480313195452E-4	0.0	0.0	22.36312916343143	0.0
33	5.938480313195452E-4	0.0	0.0	22.73339341095917	0.0
34	5.938480313195452E-4	0.0	0.0	23.09578917207192	0.0
35	5.938480313195452E-4	0.0	0.0	23.480305772351326	0.0
36	5.938480313195452E-4	0.0	0.0	23.836317667127393	0.0
37	8.907720469793177E-4	0.0	0.0	24.228702753821782	0.0
38	8.907720469793177E-4	0.0	0.0	24.701999634783462	0.0
39	8.907720469793177E-4	0.0	0.0	25.380619472573873	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	50	2.1827873E-11	45.0	4
TTACGAG	20	7.031205E-4	45.0	1
TCGACAG	20	7.031205E-4	45.0	1
CGTCATA	25	3.88908E-5	45.0	38
TGCGTTG	25	3.88908E-5	45.0	1
ATCGACG	20	7.031205E-4	45.0	1
CTACGAA	30	2.164159E-6	44.999996	11
CGTTTTT	7905	0.0	44.28843	1
CCGATGA	710	0.0	40.880283	18
ACGGGCG	45	1.9263098E-8	40.0	5
CGATGAA	720	0.0	40.0	19
TAGTGCG	45	1.9263098E-8	40.0	1
TAGCCGT	40	3.4553705E-7	39.375	44
CGGCGAA	120	0.0	39.374996	31
GTTTTTT	9090	0.0	39.034653	2
TCACGAC	35	6.244438E-6	38.571426	25
CACGACG	35	6.244438E-6	38.571426	26
GGTTACG	35	6.244438E-6	38.571426	1
CACGCCG	170	0.0	38.38235	26
TAGTAGG	135	0.0	38.333332	2
>>END_MODULE