##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935456.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 476997 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.097587615855026 31.0 31.0 33.0 28.0 34.0 2 31.20837657259899 31.0 31.0 34.0 28.0 34.0 3 30.930051970976756 31.0 31.0 34.0 27.0 34.0 4 34.623618177892105 35.0 35.0 37.0 32.0 37.0 5 34.75613473460001 35.0 35.0 37.0 32.0 37.0 6 34.96355951924226 36.0 35.0 37.0 32.0 37.0 7 35.231338981167596 37.0 35.0 37.0 33.0 37.0 8 35.1355333471699 37.0 35.0 37.0 32.0 37.0 9 37.06898995171878 39.0 37.0 39.0 33.0 39.0 10 36.47258158856345 38.0 35.0 39.0 32.0 39.0 11 36.75228565378818 39.0 37.0 39.0 32.0 39.0 12 36.841231705859784 39.0 37.0 39.0 33.0 39.0 13 36.90363251760493 39.0 37.0 39.0 33.0 39.0 14 37.926309809076365 40.0 37.0 41.0 33.0 41.0 15 38.11662337498978 40.0 37.0 41.0 33.0 41.0 16 38.07141344704474 40.0 37.0 41.0 33.0 41.0 17 37.96360983402411 40.0 37.0 41.0 33.0 41.0 18 37.810799648635104 39.0 37.0 40.0 33.0 41.0 19 37.5376700482393 39.0 37.0 40.0 33.0 41.0 20 37.43401530827238 39.0 35.0 40.0 33.0 41.0 21 37.358989679180375 39.0 35.0 40.0 33.0 41.0 22 37.45630894953218 39.0 35.0 41.0 33.0 41.0 23 37.51938691438311 39.0 35.0 41.0 33.0 41.0 24 37.45088333888892 39.0 35.0 41.0 33.0 41.0 25 37.06716813732581 39.0 35.0 41.0 32.0 41.0 26 37.10961494516737 39.0 35.0 41.0 33.0 41.0 27 37.1802212592532 39.0 35.0 41.0 33.0 41.0 28 37.047692123849835 39.0 35.0 41.0 33.0 41.0 29 36.95467686379579 39.0 35.0 41.0 32.0 41.0 30 36.62264961834141 39.0 35.0 41.0 31.0 41.0 31 36.472992492615255 39.0 35.0 40.0 31.0 41.0 32 36.26157397216335 39.0 35.0 40.0 30.0 41.0 33 36.00624322584838 39.0 35.0 41.0 29.0 41.0 34 35.665633117189415 39.0 35.0 41.0 26.0 41.0 35 35.500334383654405 39.0 35.0 41.0 25.0 41.0 36 35.28426384233025 39.0 35.0 41.0 23.0 41.0 37 35.25377727742522 39.0 35.0 41.0 23.0 41.0 38 35.06172785153785 38.0 35.0 40.0 23.0 41.0 39 34.92525948800517 38.0 35.0 40.0 23.0 41.0 40 34.82517709754988 38.0 34.0 40.0 22.0 41.0 41 34.61875860854471 38.0 34.0 40.0 21.0 41.0 42 34.63004589127395 38.0 34.0 40.0 21.0 41.0 43 34.61318415000514 38.0 34.0 40.0 21.0 41.0 44 34.57326565995174 38.0 34.0 40.0 21.0 41.0 45 34.547923781491285 38.0 34.0 40.0 21.0 41.0 46 34.44146189598677 38.0 34.0 40.0 20.0 41.0 47 34.37664178181414 38.0 34.0 40.0 20.0 41.0 48 34.275167349060894 37.0 34.0 40.0 20.0 41.0 49 34.20243942834022 37.0 34.0 40.0 20.0 41.0 50 34.07906967968352 37.0 34.0 40.0 20.0 41.0 51 32.92680247464869 36.0 32.0 39.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 13.0 9 21.0 10 28.0 11 29.0 12 16.0 13 17.0 14 33.0 15 42.0 16 87.0 17 153.0 18 259.0 19 528.0 20 813.0 21 1329.0 22 2017.0 23 3015.0 24 4632.0 25 7472.0 26 9680.0 27 10325.0 28 9901.0 29 9279.0 30 9797.0 31 11334.0 32 13959.0 33 19528.0 34 31881.0 35 37336.0 36 38947.0 37 56770.0 38 96004.0 39 101738.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.63294737702753 18.333238993117355 20.704113443061487 22.329700186793627 2 42.17028618628629 21.10684134281767 21.536194147971578 15.186678322924463 3 28.66915305546995 21.175814522942492 35.046342010536755 15.108690411050803 4 25.41944708247641 22.139761885294877 35.43774908437579 17.00304194785292 5 22.314605752237433 26.978995675025207 33.32641505082841 17.379983521908944 6 21.968691626991365 30.868537957261786 33.429769998553446 13.7330004171934 7 69.24446065698527 5.226447965081541 20.47706798994543 5.052023387987766 8 70.26752788801606 4.947829860565161 19.410394614641184 5.374247636777589 9 64.23143122493433 6.233162892009803 21.72550351469716 7.809902368358711 10 35.38617643297547 20.19844988542905 29.04462711505523 15.37074656654025 11 26.714633425367456 21.80349142657082 33.08448480808055 18.397390339981172 12 23.170166688679384 18.481038664813408 37.81386465742971 20.5349299890775 13 22.827606882223577 18.74665878401751 40.104864391180655 18.320869942578256 14 18.942886433248006 22.335989534525375 39.15726933293082 19.563854699295803 15 18.197179437187234 22.251292985071185 40.932752197602916 18.618775380138658 16 21.638081581225876 22.715446847674094 37.07801097281534 18.568460598284688 17 21.161558668083867 23.686731782380182 35.30336668784081 19.848342861695148 18 21.292796390752983 22.795321563867276 36.4333528303113 19.478529215068438 19 20.502854315645592 25.54229900816986 34.233338993746294 19.721507682438254 20 20.93870611345564 25.788841439254334 34.08491038727707 19.187542060012955 21 20.636398132483013 25.755507896276075 35.86647295475653 17.74162101648438 22 20.00641513468638 21.956741866301048 36.66102721820053 21.375815780812037 23 19.305991442294186 23.93516101778418 36.25829931844435 20.50054822147728 24 20.11396298089925 23.616291087784617 35.8448795275442 20.424866403771933 25 21.340385788589867 24.0265662048189 33.492034541097745 21.141013465493494 26 19.722975196908994 23.213143898179652 34.78386656519852 22.280014339712828 27 21.302649702199385 22.226135594144196 34.26918827581725 22.20202642783917 28 20.509143663377337 23.27289270163125 34.084071807579505 22.13389182741191 29 22.636201066254085 22.2980438032105 33.46855430956589 21.597200820969523 30 23.312725237265646 21.475187474973637 33.489518802005044 21.722568485755676 31 23.98757224888207 21.197617595079215 32.7331199148003 22.081690241238412 32 24.181284159019867 22.297205223512936 32.62242739472156 20.899083222745634 33 22.534732922848573 21.912506787254426 32.19454210403839 23.35821818585861 34 21.93577737386189 22.87792166407755 33.61488646679119 21.571414495269362 35 20.76532976098382 25.27248599047793 32.64339188716072 21.318792361377533 36 22.091124262836033 26.86620670570255 30.105640077400906 20.93702895406051 37 20.753380000293504 26.52700121803701 32.1769319303895 20.54268685127999 38 20.661136233561216 26.772914714348307 31.119692576682873 21.4462564754076 39 21.09531087197613 25.07793550064256 31.491183382704712 22.335570244676592 40 21.770786818365735 24.3320188596574 32.86142260852793 21.03577171344893 41 20.177485392989894 25.666408803409663 31.797474617240777 22.358631186359663 42 20.58776051002417 26.119661129944216 32.001459128673766 21.29111923135785 43 20.827803948452505 24.5156678134244 32.23269748027766 22.423830757845437 44 21.93032660582771 23.44522082948111 31.13583523586102 23.48861732883016 45 20.908517244343255 22.684838688712926 31.471476759811907 24.935167307131913 46 22.215024413151447 23.53578743681826 31.057428034138578 23.191760115891714 47 20.74730029748615 23.536206726667043 32.958278563596835 22.758214412249973 48 20.059036010708663 23.900569605259573 32.615299467292246 23.425094916739518 49 21.31816342660436 22.43410335914062 33.3911953324654 22.856537881789613 50 19.688593429308778 22.29930167275685 34.37023712937398 23.64186776856039 51 19.799705239236307 22.064709002362697 32.933750107443025 25.20183565095797 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 169.0 1 363.0 2 557.0 3 9843.0 4 19129.0 5 12047.0 6 4965.0 7 4771.0 8 4577.0 9 4620.0 10 4663.0 11 4534.0 12 4405.0 13 4257.5 14 4110.0 15 3863.0 16 3616.0 17 3425.5 18 3235.0 19 3109.0 20 2983.0 21 2868.0 22 2753.0 23 2789.5 24 2826.0 25 2931.5 26 3464.0 27 3891.0 28 4415.5 29 4940.0 30 5549.5 31 6159.0 32 7053.0 33 7947.0 34 9356.5 35 10766.0 36 11645.5 37 12525.0 38 13160.5 39 13796.0 40 14943.5 41 16091.0 42 18009.5 43 19928.0 44 22541.0 45 25154.0 46 34466.0 47 43778.0 48 44257.5 49 44737.0 50 43972.5 51 43208.0 52 38092.5 53 32977.0 54 29605.5 55 26234.0 56 24600.5 57 22967.0 58 21188.0 59 19409.0 60 18161.0 61 16913.0 62 15008.5 63 13104.0 64 11770.0 65 10436.0 66 8877.5 67 7319.0 68 6252.0 69 5185.0 70 4346.0 71 3507.0 72 2791.5 73 2076.0 74 1723.0 75 1088.0 76 806.0 77 588.0 78 370.0 79 285.0 80 200.0 81 153.5 82 107.0 83 70.0 84 33.0 85 23.5 86 14.0 87 14.0 88 14.0 89 9.5 90 5.0 91 5.0 92 5.0 93 3.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 476997.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.842927665075464 #Duplication Level Percentage of deduplicated Percentage of total 1 74.38205346660945 33.35509043182944 2 9.443459445252687 8.4694473762308 3 4.196262526835169 5.645180908636089 4 2.6657113401238686 4.781532032045841 5 1.9074455398860177 4.276772118508976 6 1.4293509286064463 3.8457768179704392 7 1.1448853197431113 3.5938006704632985 8 0.8770159148921205 3.146236898610187 9 0.6735317483460812 2.718282192409362 >10 3.228774332571321 21.041069638473942 >50 0.02740869131899611 0.8465859343145292 >100 0.01653972751989943 1.40878680632674 >500 0.0028353818605541883 0.8798560198358341 >1k 0.0042530727908312825 3.152605493038946 >5k 0.0 0.0 >10k+ 4.7256364342569804E-4 2.8389766613055563 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13397 2.808613052073703 No Hit GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC 2829 0.5930854911037177 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTT 2594 0.5438189338717014 No Hit CGTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC 1802 0.37778015375358753 No Hit CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG 1716 0.35975069025591355 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCT 1522 0.3190795749239513 No Hit CGTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT 1217 0.2551378729845261 No Hit GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC 1109 0.23249622115023785 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG 1079 0.2262068734184911 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC 1009 0.21153172871108206 No Hit GAACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT 892 0.18700327255726976 No Hit CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC 854 0.17903676543039054 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC 680 0.14255854858625946 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTC 605 0.1268351792568926 No Hit CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT 597 0.12515801986176012 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGT 524 0.1098539403811764 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG 483 0.10125849848112252 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0964492439155802E-4 0.0 0.0 0.13186665744228998 0.0 2 2.0964492439155802E-4 0.0 0.0 0.6224357805185358 0.0 3 2.0964492439155802E-4 0.0 0.0 1.170237967953677 0.0 4 2.0964492439155802E-4 0.0 0.0 1.9052530728704793 0.0 5 2.0964492439155802E-4 0.0 0.0 3.7117633863525348 0.0 6 2.0964492439155802E-4 0.0 0.0 5.5622991339568175 0.0 7 2.0964492439155802E-4 0.0 0.0 6.8870454111870725 0.0 8 2.0964492439155802E-4 0.0 0.0 8.626469348863829 0.0 9 2.0964492439155802E-4 0.0 0.0 9.608446174713887 0.0 10 2.0964492439155802E-4 0.0 0.0 11.091055080010985 0.0 11 2.0964492439155802E-4 0.0 0.0 12.310978895045462 0.0 12 2.0964492439155802E-4 0.0 0.0 13.448721899718446 0.0 13 2.0964492439155802E-4 0.0 0.0 14.04537135453682 0.0 14 2.0964492439155802E-4 0.0 0.0 14.394010863799982 0.0 15 2.0964492439155802E-4 0.0 0.0 14.729023452977692 0.0 16 2.0964492439155802E-4 0.0 0.0 15.141814309104669 0.0 17 2.0964492439155802E-4 0.0 0.0 15.601565628295356 0.0 18 2.0964492439155802E-4 0.0 0.0 16.142030243376794 0.0 19 2.0964492439155802E-4 0.0 0.0 16.505344897347364 0.0 20 4.1928984878311605E-4 0.0 0.0 16.852726537064175 0.0 21 4.1928984878311605E-4 0.0 0.0 17.22757166187628 0.0 22 4.1928984878311605E-4 0.0 0.0 17.653150858391143 0.0 23 4.1928984878311605E-4 0.0 0.0 18.074746801342567 0.0 24 6.289347731746741E-4 0.0 0.0 18.42128986136181 0.0 25 6.289347731746741E-4 0.0 0.0 18.75860854470783 0.0 26 6.289347731746741E-4 0.0 0.0 19.078526699329345 0.0 27 6.289347731746741E-4 0.0 0.0 19.43429413602182 0.0 28 6.289347731746741E-4 0.0 0.0 19.766162051333655 0.0 29 6.289347731746741E-4 0.0 0.0 20.130524929926185 0.0 30 6.289347731746741E-4 0.0 0.0 20.617949379136558 0.0 31 8.385796975662321E-4 0.0 0.0 20.997406692285278 0.0 32 8.385796975662321E-4 0.0 0.0 21.396989918175585 0.0 33 0.0010482246219577901 0.0 0.0 21.783155868904835 0.0 34 0.0010482246219577901 0.0 0.0 22.162613182053555 0.0 35 0.0010482246219577901 0.0 0.0 22.541651205353492 0.0 36 0.0010482246219577901 0.0 0.0 22.90182118545819 0.0 37 0.0010482246219577901 0.0 0.0 23.282326723228866 0.0 38 0.0010482246219577901 0.0 0.0 23.747528810453733 0.0 39 0.0010482246219577901 0.0 0.0 24.31231223676459 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTACGA 45 3.8380676E-10 45.000004 10 TCACCGG 20 7.029133E-4 45.0 2 TCGATGG 25 3.8873615E-5 45.0 2 CGGTCTA 35 1.2099372E-7 45.0 31 TTGCACG 20 7.029133E-4 45.0 1 TGATGCG 20 7.029133E-4 45.0 1 CGTTTTT 5660 0.0 44.20495 1 GCGATAT 45 1.9243089E-8 40.000004 9 CGACGGT 40 3.452551E-7 39.375 28 ATGCGAG 40 3.452551E-7 39.375 1 CGTAAGG 75 0.0 39.0 2 GTTTTTT 6445 0.0 38.995342 2 ACCGGGC 35 6.240607E-6 38.571426 4 AGGGCGC 135 0.0 38.333332 6 CGTGCGG 65 9.094947E-12 38.07692 2 CGTTCTG 245 0.0 37.65306 1 CGTTTCT 270 0.0 37.5 1 ATGGGCG 120 0.0 37.499996 5 ATAGCGG 60 1.546141E-10 37.499996 2 AACGGGC 60 1.546141E-10 37.499996 4 >>END_MODULE