##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935456.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476997
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.097587615855026	31.0	31.0	33.0	28.0	34.0
2	31.20837657259899	31.0	31.0	34.0	28.0	34.0
3	30.930051970976756	31.0	31.0	34.0	27.0	34.0
4	34.623618177892105	35.0	35.0	37.0	32.0	37.0
5	34.75613473460001	35.0	35.0	37.0	32.0	37.0
6	34.96355951924226	36.0	35.0	37.0	32.0	37.0
7	35.231338981167596	37.0	35.0	37.0	33.0	37.0
8	35.1355333471699	37.0	35.0	37.0	32.0	37.0
9	37.06898995171878	39.0	37.0	39.0	33.0	39.0
10	36.47258158856345	38.0	35.0	39.0	32.0	39.0
11	36.75228565378818	39.0	37.0	39.0	32.0	39.0
12	36.841231705859784	39.0	37.0	39.0	33.0	39.0
13	36.90363251760493	39.0	37.0	39.0	33.0	39.0
14	37.926309809076365	40.0	37.0	41.0	33.0	41.0
15	38.11662337498978	40.0	37.0	41.0	33.0	41.0
16	38.07141344704474	40.0	37.0	41.0	33.0	41.0
17	37.96360983402411	40.0	37.0	41.0	33.0	41.0
18	37.810799648635104	39.0	37.0	40.0	33.0	41.0
19	37.5376700482393	39.0	37.0	40.0	33.0	41.0
20	37.43401530827238	39.0	35.0	40.0	33.0	41.0
21	37.358989679180375	39.0	35.0	40.0	33.0	41.0
22	37.45630894953218	39.0	35.0	41.0	33.0	41.0
23	37.51938691438311	39.0	35.0	41.0	33.0	41.0
24	37.45088333888892	39.0	35.0	41.0	33.0	41.0
25	37.06716813732581	39.0	35.0	41.0	32.0	41.0
26	37.10961494516737	39.0	35.0	41.0	33.0	41.0
27	37.1802212592532	39.0	35.0	41.0	33.0	41.0
28	37.047692123849835	39.0	35.0	41.0	33.0	41.0
29	36.95467686379579	39.0	35.0	41.0	32.0	41.0
30	36.62264961834141	39.0	35.0	41.0	31.0	41.0
31	36.472992492615255	39.0	35.0	40.0	31.0	41.0
32	36.26157397216335	39.0	35.0	40.0	30.0	41.0
33	36.00624322584838	39.0	35.0	41.0	29.0	41.0
34	35.665633117189415	39.0	35.0	41.0	26.0	41.0
35	35.500334383654405	39.0	35.0	41.0	25.0	41.0
36	35.28426384233025	39.0	35.0	41.0	23.0	41.0
37	35.25377727742522	39.0	35.0	41.0	23.0	41.0
38	35.06172785153785	38.0	35.0	40.0	23.0	41.0
39	34.92525948800517	38.0	35.0	40.0	23.0	41.0
40	34.82517709754988	38.0	34.0	40.0	22.0	41.0
41	34.61875860854471	38.0	34.0	40.0	21.0	41.0
42	34.63004589127395	38.0	34.0	40.0	21.0	41.0
43	34.61318415000514	38.0	34.0	40.0	21.0	41.0
44	34.57326565995174	38.0	34.0	40.0	21.0	41.0
45	34.547923781491285	38.0	34.0	40.0	21.0	41.0
46	34.44146189598677	38.0	34.0	40.0	20.0	41.0
47	34.37664178181414	38.0	34.0	40.0	20.0	41.0
48	34.275167349060894	37.0	34.0	40.0	20.0	41.0
49	34.20243942834022	37.0	34.0	40.0	20.0	41.0
50	34.07906967968352	37.0	34.0	40.0	20.0	41.0
51	32.92680247464869	36.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	21.0
10	28.0
11	29.0
12	16.0
13	17.0
14	33.0
15	42.0
16	87.0
17	153.0
18	259.0
19	528.0
20	813.0
21	1329.0
22	2017.0
23	3015.0
24	4632.0
25	7472.0
26	9680.0
27	10325.0
28	9901.0
29	9279.0
30	9797.0
31	11334.0
32	13959.0
33	19528.0
34	31881.0
35	37336.0
36	38947.0
37	56770.0
38	96004.0
39	101738.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.63294737702753	18.333238993117355	20.704113443061487	22.329700186793627
2	42.17028618628629	21.10684134281767	21.536194147971578	15.186678322924463
3	28.66915305546995	21.175814522942492	35.046342010536755	15.108690411050803
4	25.41944708247641	22.139761885294877	35.43774908437579	17.00304194785292
5	22.314605752237433	26.978995675025207	33.32641505082841	17.379983521908944
6	21.968691626991365	30.868537957261786	33.429769998553446	13.7330004171934
7	69.24446065698527	5.226447965081541	20.47706798994543	5.052023387987766
8	70.26752788801606	4.947829860565161	19.410394614641184	5.374247636777589
9	64.23143122493433	6.233162892009803	21.72550351469716	7.809902368358711
10	35.38617643297547	20.19844988542905	29.04462711505523	15.37074656654025
11	26.714633425367456	21.80349142657082	33.08448480808055	18.397390339981172
12	23.170166688679384	18.481038664813408	37.81386465742971	20.5349299890775
13	22.827606882223577	18.74665878401751	40.104864391180655	18.320869942578256
14	18.942886433248006	22.335989534525375	39.15726933293082	19.563854699295803
15	18.197179437187234	22.251292985071185	40.932752197602916	18.618775380138658
16	21.638081581225876	22.715446847674094	37.07801097281534	18.568460598284688
17	21.161558668083867	23.686731782380182	35.30336668784081	19.848342861695148
18	21.292796390752983	22.795321563867276	36.4333528303113	19.478529215068438
19	20.502854315645592	25.54229900816986	34.233338993746294	19.721507682438254
20	20.93870611345564	25.788841439254334	34.08491038727707	19.187542060012955
21	20.636398132483013	25.755507896276075	35.86647295475653	17.74162101648438
22	20.00641513468638	21.956741866301048	36.66102721820053	21.375815780812037
23	19.305991442294186	23.93516101778418	36.25829931844435	20.50054822147728
24	20.11396298089925	23.616291087784617	35.8448795275442	20.424866403771933
25	21.340385788589867	24.0265662048189	33.492034541097745	21.141013465493494
26	19.722975196908994	23.213143898179652	34.78386656519852	22.280014339712828
27	21.302649702199385	22.226135594144196	34.26918827581725	22.20202642783917
28	20.509143663377337	23.27289270163125	34.084071807579505	22.13389182741191
29	22.636201066254085	22.2980438032105	33.46855430956589	21.597200820969523
30	23.312725237265646	21.475187474973637	33.489518802005044	21.722568485755676
31	23.98757224888207	21.197617595079215	32.7331199148003	22.081690241238412
32	24.181284159019867	22.297205223512936	32.62242739472156	20.899083222745634
33	22.534732922848573	21.912506787254426	32.19454210403839	23.35821818585861
34	21.93577737386189	22.87792166407755	33.61488646679119	21.571414495269362
35	20.76532976098382	25.27248599047793	32.64339188716072	21.318792361377533
36	22.091124262836033	26.86620670570255	30.105640077400906	20.93702895406051
37	20.753380000293504	26.52700121803701	32.1769319303895	20.54268685127999
38	20.661136233561216	26.772914714348307	31.119692576682873	21.4462564754076
39	21.09531087197613	25.07793550064256	31.491183382704712	22.335570244676592
40	21.770786818365735	24.3320188596574	32.86142260852793	21.03577171344893
41	20.177485392989894	25.666408803409663	31.797474617240777	22.358631186359663
42	20.58776051002417	26.119661129944216	32.001459128673766	21.29111923135785
43	20.827803948452505	24.5156678134244	32.23269748027766	22.423830757845437
44	21.93032660582771	23.44522082948111	31.13583523586102	23.48861732883016
45	20.908517244343255	22.684838688712926	31.471476759811907	24.935167307131913
46	22.215024413151447	23.53578743681826	31.057428034138578	23.191760115891714
47	20.74730029748615	23.536206726667043	32.958278563596835	22.758214412249973
48	20.059036010708663	23.900569605259573	32.615299467292246	23.425094916739518
49	21.31816342660436	22.43410335914062	33.3911953324654	22.856537881789613
50	19.688593429308778	22.29930167275685	34.37023712937398	23.64186776856039
51	19.799705239236307	22.064709002362697	32.933750107443025	25.20183565095797
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	363.0
2	557.0
3	9843.0
4	19129.0
5	12047.0
6	4965.0
7	4771.0
8	4577.0
9	4620.0
10	4663.0
11	4534.0
12	4405.0
13	4257.5
14	4110.0
15	3863.0
16	3616.0
17	3425.5
18	3235.0
19	3109.0
20	2983.0
21	2868.0
22	2753.0
23	2789.5
24	2826.0
25	2931.5
26	3464.0
27	3891.0
28	4415.5
29	4940.0
30	5549.5
31	6159.0
32	7053.0
33	7947.0
34	9356.5
35	10766.0
36	11645.5
37	12525.0
38	13160.5
39	13796.0
40	14943.5
41	16091.0
42	18009.5
43	19928.0
44	22541.0
45	25154.0
46	34466.0
47	43778.0
48	44257.5
49	44737.0
50	43972.5
51	43208.0
52	38092.5
53	32977.0
54	29605.5
55	26234.0
56	24600.5
57	22967.0
58	21188.0
59	19409.0
60	18161.0
61	16913.0
62	15008.5
63	13104.0
64	11770.0
65	10436.0
66	8877.5
67	7319.0
68	6252.0
69	5185.0
70	4346.0
71	3507.0
72	2791.5
73	2076.0
74	1723.0
75	1088.0
76	806.0
77	588.0
78	370.0
79	285.0
80	200.0
81	153.5
82	107.0
83	70.0
84	33.0
85	23.5
86	14.0
87	14.0
88	14.0
89	9.5
90	5.0
91	5.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	476997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.842927665075464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.38205346660945	33.35509043182944
2	9.443459445252687	8.4694473762308
3	4.196262526835169	5.645180908636089
4	2.6657113401238686	4.781532032045841
5	1.9074455398860177	4.276772118508976
6	1.4293509286064463	3.8457768179704392
7	1.1448853197431113	3.5938006704632985
8	0.8770159148921205	3.146236898610187
9	0.6735317483460812	2.718282192409362
>10	3.228774332571321	21.041069638473942
>50	0.02740869131899611	0.8465859343145292
>100	0.01653972751989943	1.40878680632674
>500	0.0028353818605541883	0.8798560198358341
>1k	0.0042530727908312825	3.152605493038946
>5k	0.0	0.0
>10k+	4.7256364342569804E-4	2.8389766613055563
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13397	2.808613052073703	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC	2829	0.5930854911037177	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTT	2594	0.5438189338717014	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC	1802	0.37778015375358753	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	1716	0.35975069025591355	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCT	1522	0.3190795749239513	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT	1217	0.2551378729845261	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1109	0.23249622115023785	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	1079	0.2262068734184911	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC	1009	0.21153172871108206	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT	892	0.18700327255726976	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	854	0.17903676543039054	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC	680	0.14255854858625946	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTC	605	0.1268351792568926	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	597	0.12515801986176012	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGT	524	0.1098539403811764	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	483	0.10125849848112252	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0964492439155802E-4	0.0	0.0	0.13186665744228998	0.0
2	2.0964492439155802E-4	0.0	0.0	0.6224357805185358	0.0
3	2.0964492439155802E-4	0.0	0.0	1.170237967953677	0.0
4	2.0964492439155802E-4	0.0	0.0	1.9052530728704793	0.0
5	2.0964492439155802E-4	0.0	0.0	3.7117633863525348	0.0
6	2.0964492439155802E-4	0.0	0.0	5.5622991339568175	0.0
7	2.0964492439155802E-4	0.0	0.0	6.8870454111870725	0.0
8	2.0964492439155802E-4	0.0	0.0	8.626469348863829	0.0
9	2.0964492439155802E-4	0.0	0.0	9.608446174713887	0.0
10	2.0964492439155802E-4	0.0	0.0	11.091055080010985	0.0
11	2.0964492439155802E-4	0.0	0.0	12.310978895045462	0.0
12	2.0964492439155802E-4	0.0	0.0	13.448721899718446	0.0
13	2.0964492439155802E-4	0.0	0.0	14.04537135453682	0.0
14	2.0964492439155802E-4	0.0	0.0	14.394010863799982	0.0
15	2.0964492439155802E-4	0.0	0.0	14.729023452977692	0.0
16	2.0964492439155802E-4	0.0	0.0	15.141814309104669	0.0
17	2.0964492439155802E-4	0.0	0.0	15.601565628295356	0.0
18	2.0964492439155802E-4	0.0	0.0	16.142030243376794	0.0
19	2.0964492439155802E-4	0.0	0.0	16.505344897347364	0.0
20	4.1928984878311605E-4	0.0	0.0	16.852726537064175	0.0
21	4.1928984878311605E-4	0.0	0.0	17.22757166187628	0.0
22	4.1928984878311605E-4	0.0	0.0	17.653150858391143	0.0
23	4.1928984878311605E-4	0.0	0.0	18.074746801342567	0.0
24	6.289347731746741E-4	0.0	0.0	18.42128986136181	0.0
25	6.289347731746741E-4	0.0	0.0	18.75860854470783	0.0
26	6.289347731746741E-4	0.0	0.0	19.078526699329345	0.0
27	6.289347731746741E-4	0.0	0.0	19.43429413602182	0.0
28	6.289347731746741E-4	0.0	0.0	19.766162051333655	0.0
29	6.289347731746741E-4	0.0	0.0	20.130524929926185	0.0
30	6.289347731746741E-4	0.0	0.0	20.617949379136558	0.0
31	8.385796975662321E-4	0.0	0.0	20.997406692285278	0.0
32	8.385796975662321E-4	0.0	0.0	21.396989918175585	0.0
33	0.0010482246219577901	0.0	0.0	21.783155868904835	0.0
34	0.0010482246219577901	0.0	0.0	22.162613182053555	0.0
35	0.0010482246219577901	0.0	0.0	22.541651205353492	0.0
36	0.0010482246219577901	0.0	0.0	22.90182118545819	0.0
37	0.0010482246219577901	0.0	0.0	23.282326723228866	0.0
38	0.0010482246219577901	0.0	0.0	23.747528810453733	0.0
39	0.0010482246219577901	0.0	0.0	24.31231223676459	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	45	3.8380676E-10	45.000004	10
TCACCGG	20	7.029133E-4	45.0	2
TCGATGG	25	3.8873615E-5	45.0	2
CGGTCTA	35	1.2099372E-7	45.0	31
TTGCACG	20	7.029133E-4	45.0	1
TGATGCG	20	7.029133E-4	45.0	1
CGTTTTT	5660	0.0	44.20495	1
GCGATAT	45	1.9243089E-8	40.000004	9
CGACGGT	40	3.452551E-7	39.375	28
ATGCGAG	40	3.452551E-7	39.375	1
CGTAAGG	75	0.0	39.0	2
GTTTTTT	6445	0.0	38.995342	2
ACCGGGC	35	6.240607E-6	38.571426	4
AGGGCGC	135	0.0	38.333332	6
CGTGCGG	65	9.094947E-12	38.07692	2
CGTTCTG	245	0.0	37.65306	1
CGTTTCT	270	0.0	37.5	1
ATGGGCG	120	0.0	37.499996	5
ATAGCGG	60	1.546141E-10	37.499996	2
AACGGGC	60	1.546141E-10	37.499996	4
>>END_MODULE