##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935455.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573441
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197326316046464	31.0	31.0	33.0	28.0	34.0
2	31.247451786670293	31.0	31.0	34.0	28.0	34.0
3	31.007451507652924	31.0	31.0	34.0	27.0	34.0
4	34.751006642357275	35.0	35.0	37.0	32.0	37.0
5	34.83041149830584	35.0	35.0	37.0	33.0	37.0
6	35.05245875338527	37.0	35.0	37.0	32.0	37.0
7	35.410795530839266	37.0	35.0	37.0	33.0	37.0
8	35.245934629717794	37.0	35.0	37.0	32.0	37.0
9	37.21330354822902	39.0	37.0	39.0	34.0	39.0
10	36.49957362658059	38.0	35.0	39.0	32.0	39.0
11	36.86992035797929	39.0	37.0	39.0	33.0	39.0
12	37.001440427175595	39.0	37.0	39.0	33.0	39.0
13	37.075768910838256	39.0	37.0	39.0	33.0	39.0
14	38.129931414042595	40.0	38.0	41.0	33.0	41.0
15	38.31658008408886	40.0	38.0	41.0	34.0	41.0
16	38.24621539094693	40.0	37.0	41.0	34.0	41.0
17	38.173191313491714	40.0	37.0	41.0	34.0	41.0
18	38.07351758942943	39.0	37.0	41.0	34.0	41.0
19	37.852410274117126	39.0	37.0	41.0	34.0	41.0
20	37.87409864310365	39.0	36.0	41.0	34.0	41.0
21	37.78480436522676	39.0	36.0	41.0	33.0	41.0
22	37.90736623296904	40.0	36.0	41.0	34.0	41.0
23	37.954422512516544	40.0	36.0	41.0	34.0	41.0
24	37.94177779405379	40.0	36.0	41.0	34.0	41.0
25	37.51166728573646	39.0	35.0	41.0	33.0	41.0
26	37.64031347601584	39.0	35.0	41.0	33.0	41.0
27	37.72242828817612	40.0	36.0	41.0	33.0	41.0
28	37.59499931117587	40.0	35.0	41.0	33.0	41.0
29	37.53725666633533	40.0	35.0	41.0	33.0	41.0
30	37.23079619350552	39.0	35.0	41.0	33.0	41.0
31	37.14497219417516	39.0	35.0	41.0	32.0	41.0
32	37.030393710948466	39.0	35.0	41.0	32.0	41.0
33	36.87373417666333	40.0	35.0	41.0	31.0	41.0
34	36.65154915675719	40.0	35.0	41.0	31.0	41.0
35	36.581320484583415	40.0	35.0	41.0	30.0	41.0
36	36.40690498237831	40.0	35.0	41.0	30.0	41.0
37	36.3967731641093	40.0	35.0	41.0	30.0	41.0
38	36.230053309756364	39.0	35.0	41.0	29.0	41.0
39	36.14063347406272	39.0	35.0	41.0	29.0	41.0
40	36.05573720748952	39.0	35.0	41.0	29.0	41.0
41	35.88310567259753	39.0	35.0	41.0	27.0	41.0
42	35.91321687845829	39.0	35.0	41.0	28.0	41.0
43	35.88857790077793	39.0	35.0	41.0	28.0	41.0
44	35.87224143373076	39.0	35.0	41.0	28.0	41.0
45	35.8677387909131	39.0	35.0	41.0	28.0	41.0
46	35.75079737932935	39.0	35.0	41.0	27.0	41.0
47	35.75881215329912	39.0	35.0	41.0	27.0	41.0
48	35.66968877356171	39.0	35.0	41.0	27.0	41.0
49	35.6383202456748	39.0	35.0	41.0	27.0	41.0
50	35.53308884436237	39.0	35.0	41.0	27.0	41.0
51	34.47145565106088	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	9.0
9	20.0
10	25.0
11	14.0
12	14.0
13	14.0
14	14.0
15	36.0
16	57.0
17	133.0
18	221.0
19	412.0
20	684.0
21	1114.0
22	1619.0
23	2568.0
24	3931.0
25	6193.0
26	8353.0
27	8993.0
28	8760.0
29	8575.0
30	9636.0
31	11247.0
32	14313.0
33	20100.0
34	33492.0
35	40108.0
36	45237.0
37	68817.0
38	125227.0
39	153485.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.84444955976291	20.13180083042545	23.063401465887512	17.960348143924136
2	38.70877736332072	21.390343557576106	23.716476498890035	16.184402580213135
3	30.857228555335247	21.339771659159357	31.530532347704472	16.272467437800923
4	26.909655919266324	24.269454050198714	30.46451160625069	18.356378424284276
5	22.964524685189932	28.753611967055026	29.74203100231759	18.539832345437457
6	22.730498865620003	33.24474531817572	29.65291982959014	14.371835986614142
7	75.0007411398906	5.522625692965797	14.83674868033503	4.639884486808582
8	76.10704501422117	4.913844667542084	14.201112232993454	4.777998085243294
9	69.47410457222277	7.691113819904751	16.740170305227565	6.0946113026449105
10	31.264593916375006	29.08668895317914	24.886082439169854	14.762634691276
11	27.64120458774312	24.875793673629897	29.522479208846246	17.960522529780746
12	24.463022351035242	21.16974544896511	34.313905005048476	20.05332719495118
13	23.269351162543312	21.258507849979335	36.39886230667148	19.07327868080587
14	19.807791908845022	24.529463362403455	35.53007196904302	20.1326727597085
15	18.889301602082863	24.633571718799317	37.16511376061356	19.312012918504255
16	21.766145078569547	24.437736401826864	34.48951156265422	19.306606956949363
17	22.076028745764603	24.06385312525613	32.54371417460558	21.316403954373683
18	22.07323857205885	23.751179284355324	33.895030177472485	20.280551966113343
19	21.939310234182766	25.84206570510305	32.17767826158227	20.040945799131908
20	21.95936460769286	26.115328342410116	31.935281920895086	19.990025129001936
21	21.75219421004079	25.93204880711355	33.652110679215475	18.66364630363019
22	20.555732847843107	23.99008790791032	33.55480337122738	21.899375873019196
23	19.16221546767671	25.37924564166148	33.84550459419539	21.61303429646642
24	20.01600862163675	24.624154882542406	33.408668023388636	21.95116847243221
25	20.43348836235986	26.07016240554826	31.644406312070466	21.851942920021415
26	19.522322261575297	25.485272242480043	32.3914753217855	22.600930174159156
27	20.40959750000436	24.516384423157746	32.4962812216078	22.577736855230093
28	19.423794252590938	25.428771224938572	32.96380970317783	22.183624819292653
29	20.91078245190002	24.41663571317712	31.97905277090407	22.69352906401879
30	21.943321108884785	23.759375419615967	32.48651561363767	21.810787857861577
31	21.196600870882968	26.29302753029518	30.653545874815368	21.85682572400648
32	22.261400911340488	26.24873352271637	30.27390786497652	21.21595770096662
33	22.64051576360951	25.141383333246143	30.698711811677228	21.519389091467126
34	21.26373942567762	26.543271234529797	30.470266339518798	21.722723000273785
35	21.03302693738327	26.997546390997506	30.373830960813752	21.59559571080547
36	21.599780971364098	28.370486240083988	29.92757755375008	20.102155234801835
37	21.054999555316066	28.635203970417184	30.35273027216401	19.957066202102745
38	21.871474135961677	28.208307393437167	29.635132472215975	20.28508599838519
39	22.671905217799214	25.92088811229054	29.698783309878436	21.70842336003181
40	22.57512106738095	25.929956176834235	31.028824238238983	20.466098517545834
41	19.87998765348135	26.855770689573994	30.959941824878236	22.30429983206642
42	20.489640608188115	26.585646997685902	31.579883545124954	21.34482884900103
43	20.900319300503455	25.539331858028984	32.14960213866814	21.410746702799415
44	22.003484229415058	25.18637488425139	30.30372784645674	22.50641303987681
45	21.67651074827227	24.231961091027674	29.912754755938277	24.17877340476178
46	21.68034723711768	25.670818793912538	29.874564253340797	22.774269715628982
47	20.16842186031344	25.922631970856635	31.613714401307195	22.29523176752273
48	20.56497529824341	25.06221215434543	31.600809847918093	22.77200269949306
49	21.56141608291001	24.221497939631103	31.726018892963705	22.491067084495178
50	20.167026773460563	23.967766518264302	32.19930210780185	23.665904600473283
51	20.07094016646874	23.80279749791173	30.963080770297207	25.163181565322322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	263.0
1	491.5
2	720.0
3	7864.5
4	15009.0
5	9410.0
6	3811.0
7	3695.0
8	3579.0
9	3552.5
10	3526.0
11	3547.0
12	3568.0
13	3394.5
14	3221.0
15	3109.0
16	2997.0
17	2895.5
18	2794.0
19	2709.5
20	2625.0
21	2667.0
22	2709.0
23	2865.0
24	3021.0
25	3401.0
26	4383.0
27	4985.0
28	5729.5
29	6474.0
30	7429.0
31	8384.0
32	10006.5
33	11629.0
34	12952.5
35	14276.0
36	15951.0
37	17626.0
38	19040.5
39	20455.0
40	23436.0
41	26417.0
42	29765.5
43	33114.0
44	40734.5
45	48355.0
46	52647.0
47	56939.0
48	56700.5
49	56462.0
50	50306.5
51	44151.0
52	40274.0
53	36397.0
54	32641.0
55	28885.0
56	27356.5
57	25828.0
58	23883.0
59	21938.0
60	19971.0
61	18004.0
62	16301.5
63	14599.0
64	12262.5
65	9926.0
66	7912.0
67	5898.0
68	5027.5
69	4157.0
70	3376.0
71	2595.0
72	2217.5
73	1840.0
74	1502.5
75	941.0
76	717.0
77	527.5
78	338.0
79	246.5
80	155.0
81	104.5
82	54.0
83	38.0
84	22.0
85	20.5
86	19.0
87	12.5
88	6.0
89	4.5
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	573441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.24768557865233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9755899173461	30.51321873406015
2	9.363461697564231	7.7244224805776724
3	3.7904303879710564	4.690394425523979
4	2.3402471002669474	3.8611910627265558
5	1.6808199552982017	3.4664966515232347
6	1.3079684988030909	3.2370404031247064
7	1.0589185970993564	3.057455894257932
8	0.9122414055273398	3.0102277333615666
9	0.7491852441785374	2.781194165283796
>10	4.749037052140439	31.083786183758395
>50	0.04650245962040834	1.2951701477186848
>100	0.020478147347441085	1.4994761990662955
>500	0.00213314034869178	0.6284038853263398
>1k	0.0025597684184301356	1.330013039847795
>5k	0.0	0.0
>10k+	4.266280697383559E-4	1.8215089938428852
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10351	1.8050680017647849	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT	1691	0.29488648352664004	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG	1459	0.25442896479323945	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	1228	0.21414583191644823	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	1120	0.1953121594026238	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	1059	0.1846746221494452	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	1001	0.17456024246609503	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	903	0.15747042851836543	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	819	0.14282201656316865	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	668	0.11648975221513634	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC	650	0.11335080679616562	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.487717132189711E-4	0.0	0.0	0.15014622254076707	0.0
2	3.487717132189711E-4	0.0	0.0	0.645402055311706	0.0
3	3.487717132189711E-4	0.0	0.0	1.1167670257271454	0.0
4	3.487717132189711E-4	0.0	0.0	1.66102528420535	0.0
5	3.487717132189711E-4	0.0	0.0	2.9288104617563095	0.0
6	3.487717132189711E-4	0.0	0.0	4.336453096308077	0.0
7	3.487717132189711E-4	0.0	0.0	5.370212454289107	0.0
8	3.487717132189711E-4	0.0	0.0	6.897309400618372	0.0
9	3.487717132189711E-4	0.0	0.0	7.659724365715043	0.0
10	3.487717132189711E-4	0.0	0.0	8.96587443172009	0.0
11	3.487717132189711E-4	0.0	0.0	10.35189322005228	0.0
12	3.487717132189711E-4	0.0	0.0	11.48139041331192	0.0
13	3.487717132189711E-4	0.0	0.0	12.070117065225542	0.0
14	3.487717132189711E-4	0.0	0.0	12.385581079832102	0.0
15	5.231575698284567E-4	0.0	0.0	12.716739821533514	0.0
16	6.975434264379422E-4	0.0	0.0	13.371384327245524	0.0
17	6.975434264379422E-4	0.0	0.0	14.041026016625947	0.0
18	6.975434264379422E-4	0.0	0.0	14.804312911005665	0.0
19	6.975434264379422E-4	0.0	0.0	15.247776144363588	0.0
20	6.975434264379422E-4	0.0	0.0	15.748961096259249	0.0
21	6.975434264379422E-4	0.0	0.0	16.30403127784724	0.0
22	6.975434264379422E-4	0.0	0.0	16.886131267209706	0.0
23	6.975434264379422E-4	0.0	0.0	17.45079267091122	0.0
24	6.975434264379422E-4	0.0	0.0	17.900010637537253	0.0
25	6.975434264379422E-4	0.0	0.0	18.349228604163287	0.0
26	6.975434264379422E-4	0.0	0.0	18.76897536102232	0.0
27	6.975434264379422E-4	0.0	0.0	19.20511438840264	0.0
28	6.975434264379422E-4	0.0	0.0	19.638288856220605	0.0
29	6.975434264379422E-4	0.0	0.0	20.086460507706985	0.0
30	6.975434264379422E-4	0.0	0.0	20.639263673159054	0.0
31	6.975434264379422E-4	0.0	0.0	21.099642334608095	0.0
32	8.719292830474277E-4	0.0	0.0	21.536478905414857	0.0
33	8.719292830474277E-4	0.0	0.0	21.98552248618428	0.0
34	8.719292830474277E-4	0.0	0.0	22.45026079404856	0.0
35	8.719292830474277E-4	0.0	0.0	22.93069382900769	0.0
36	8.719292830474277E-4	0.0	0.0	23.38601530061506	0.0
37	8.719292830474277E-4	0.0	0.0	23.837325897520408	0.0
38	8.719292830474277E-4	0.0	0.0	24.33484874642727	0.0
39	8.719292830474277E-4	0.0	0.0	24.922703469057847	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	35	1.210501E-7	45.000004	16
AACTCGA	30	2.1635915E-6	45.000004	24
AGTAGCG	25	3.8883525E-5	45.0	1
CGTTTTT	4305	0.0	43.37979	1
TTGTGCG	55	6.002665E-11	40.909092	1
CGTTTCT	220	0.0	40.909092	1
ATTACGG	45	1.9254003E-8	40.000004	2
CGCACGG	45	1.9254003E-8	40.000004	2
TACCGTG	40	3.454188E-7	39.375	1
GGCCGAT	70	0.0	38.57143	8
TCGAGTT	35	6.2428135E-6	38.57143	27
GCGTAAG	65	9.094947E-12	38.07692	1
CGCGGTA	30	1.13920825E-4	37.500004	31
CGAAAGG	60	1.546141E-10	37.500004	2
AACGAAG	30	1.13920825E-4	37.500004	1
CATCGAC	30	1.13920825E-4	37.500004	32
CGTAAGG	165	0.0	36.81818	2
TTTAGCG	50	4.8716174E-8	36.0	1
TCGTTTA	25	0.0021062302	36.0	38
CGCATCG	25	0.0021062302	36.0	21
>>END_MODULE