FastQCFastQC Report
Sat 14 Jan 2017
SRR2935454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124008
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC12120.9773562995935746No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC12020.9692923037223405No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG11380.9176827301464421No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC11060.891877943358493No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.4862589510354171No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC6020.48545255144829363No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT5080.40965099025869295No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT2810.2265982839816786No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT2800.22579188439455516No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC2730.22014708728469135No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT2360.1903103025611251No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT2310.18627830462550804No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA2230.17982710792852075No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC2090.16853751370879297No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC2060.16611831494742274No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC1840.1483775240307077No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1590.12821753435262243No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG1590.12821753435262243No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG1320.1064447455002903No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG253.8706377E-545.0000046
GGAATGC302.1498145E-645.0000048
CGTTGGG253.8706377E-545.0000043
TACGGGA302.1498145E-645.0000044
ACGGGTA253.8706377E-545.0000045
CCTCGAT302.1498145E-645.00000415
ACGGGCT302.1498145E-645.0000045
CAGGGAG502.1827873E-1145.0000045
AGACGGG502.1827873E-1145.0000043
CGGGCAT302.1498145E-645.0000046
TCGTCGG302.1498145E-645.0000042
GCCTCGA302.1498145E-645.00000414
CGGGATA253.8706377E-545.0000046
CTCGATC302.1498145E-645.00000416
CGATCAG302.1498145E-645.00000418
GAGTGGG207.0089404E-445.03
ACTATGG207.0089404E-445.02
AATTTGC207.0089404E-445.011
CGTGCGG207.0089404E-445.02
ACGTTAA207.0089404E-445.021
Produced by FastQC (version 0.11.5)