##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935454.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124008
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.031933423650088	31.0	30.0	33.0	28.0	34.0
2	31.039199083930068	31.0	31.0	34.0	28.0	34.0
3	30.855751241855366	31.0	30.0	34.0	27.0	34.0
4	34.69117315011935	35.0	35.0	37.0	32.0	37.0
5	34.72618702019225	35.0	35.0	37.0	32.0	37.0
6	34.92598058189794	36.0	35.0	37.0	32.0	37.0
7	35.3135523514612	37.0	35.0	37.0	33.0	37.0
8	35.07277756273789	37.0	35.0	37.0	32.0	37.0
9	36.82597896909877	39.0	37.0	39.0	32.0	39.0
10	36.269168118185924	37.0	35.0	39.0	32.0	39.0
11	36.53228017547255	38.0	35.0	39.0	32.0	39.0
12	36.537078253015935	38.0	35.0	39.0	33.0	39.0
13	36.40800593510096	39.0	35.0	39.0	32.0	39.0
14	37.34749371008322	39.0	36.0	40.0	32.0	41.0
15	37.58977646603445	39.0	36.0	41.0	33.0	41.0
16	37.61811334752596	39.0	36.0	41.0	33.0	41.0
17	37.559665505451264	39.0	36.0	40.0	33.0	41.0
18	37.595711566995675	39.0	36.0	40.0	33.0	41.0
19	37.43225437068576	39.0	36.0	40.0	33.0	41.0
20	37.454478743306886	39.0	35.0	40.0	33.0	41.0
21	37.462994322946905	39.0	35.0	40.0	33.0	41.0
22	37.607549512934646	39.0	35.0	41.0	33.0	41.0
23	37.64055544803561	39.0	35.0	41.0	33.0	41.0
24	37.65475614476485	39.0	35.0	41.0	33.0	41.0
25	37.27095026127347	39.0	35.0	41.0	33.0	41.0
26	37.31284271982453	39.0	35.0	41.0	33.0	41.0
27	37.29943229469067	39.0	35.0	41.0	33.0	41.0
28	37.17715792529514	39.0	35.0	41.0	32.0	41.0
29	37.14348267853687	39.0	35.0	41.0	32.0	41.0
30	36.90670763176569	39.0	35.0	41.0	31.0	41.0
31	36.86191213470099	39.0	35.0	40.0	31.0	41.0
32	36.87177440165151	39.0	35.0	40.0	32.0	41.0
33	36.77351461196052	39.0	35.0	41.0	31.0	41.0
34	36.64025708018838	39.0	35.0	41.0	31.0	41.0
35	36.70386587962067	39.0	35.0	41.0	31.0	41.0
36	36.52175666086059	39.0	35.0	41.0	31.0	41.0
37	36.484355848009805	39.0	35.0	41.0	31.0	41.0
38	36.29302948196891	39.0	35.0	40.0	30.0	41.0
39	36.19926133797819	39.0	35.0	40.0	30.0	41.0
40	36.08084155860912	39.0	35.0	40.0	30.0	41.0
41	35.88720888974905	38.0	35.0	40.0	29.0	41.0
42	36.00754790013548	38.0	35.0	40.0	29.0	41.0
43	35.93332688213663	38.0	35.0	40.0	29.0	41.0
44	35.89623250112896	38.0	35.0	40.0	29.0	41.0
45	35.92985129991614	38.0	35.0	40.0	29.0	41.0
46	35.81882620476098	38.0	35.0	40.0	29.0	41.0
47	35.742169860009035	38.0	35.0	40.0	29.0	41.0
48	35.716768273014644	38.0	35.0	40.0	29.0	41.0
49	35.68577027288562	38.0	35.0	40.0	29.0	41.0
50	35.48653312689504	38.0	35.0	40.0	28.0	41.0
51	34.156989871621185	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	10.0
11	6.0
12	6.0
13	3.0
14	7.0
15	9.0
16	11.0
17	18.0
18	32.0
19	98.0
20	215.0
21	299.0
22	473.0
23	648.0
24	782.0
25	1019.0
26	1182.0
27	1351.0
28	1479.0
29	1803.0
30	2256.0
31	2925.0
32	3991.0
33	5499.0
34	10298.0
35	11219.0
36	10076.0
37	15330.0
38	26311.0
39	26636.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60289658731695	20.63899103283659	24.079091671505065	12.6790207083414
2	31.322979162634667	26.95955099670989	25.417714986129923	16.299754854525514
3	29.981130249661312	26.366847300174186	27.065189342623057	16.586833107541448
4	26.95793819753564	23.583155925424165	30.73027546609896	18.72863041094123
5	25.676569253596544	27.713534610670283	26.454744855170638	20.155151280562546
6	22.997709825172567	34.944519708405906	26.929714211986322	15.128056254435199
7	73.25978969098767	5.933488162054061	15.03209470356751	5.774627443390749
8	72.4098445261596	8.021256693116573	12.04841623121089	7.520482549512934
9	68.0044835817044	6.499580672214695	14.295851880523838	11.20008386555706
10	39.62486291207019	23.249306496355075	21.27685310625121	15.848977485323529
11	30.85042900458035	24.346009934842915	26.552319205212566	18.25124185536417
12	28.29252951422489	19.786626669247145	31.56893103670731	20.35191277982066
13	24.005709309076835	25.081446358299463	33.591381201212826	17.321463131410876
14	17.519031030256112	32.030998000129024	29.183601057996256	21.266369911618604
15	14.93452035352558	24.23633959099413	42.04728727178892	18.781852783691374
16	17.610154183601058	24.075866073156572	33.55589961937939	24.758080123862978
17	17.892394039094253	23.826688600735434	30.818979420682535	27.461937939487775
18	19.919682601122506	23.675085478356237	32.99222630798013	23.413005612541127
19	20.757531772143732	26.301528933617185	28.885233210760596	24.055706083478483
20	21.953422359847753	26.104767434359072	30.782691439261985	21.15911876653119
21	20.26320882523708	28.97313076575705	31.509257467260177	19.254402941745692
22	17.758531707631768	24.468582672085674	29.634378427198243	28.138507193084315
23	17.501290239339397	27.596606670537383	29.570672859815495	25.33143023030772
24	20.973646861492806	23.949261337978196	30.357718856847942	24.719372943681055
25	18.205277078898135	28.272369524546804	28.815882846267982	24.70647055028708
26	18.429456164118445	26.171698600090316	30.848816205406106	24.550029030385137
27	21.004290045803497	28.570737371782467	28.270756725372557	22.154215857041482
28	17.292432746274432	27.569995484162312	31.96164763563641	23.17592413392684
29	20.984130056125412	24.09683246242178	31.742306947938843	23.176730533513968
30	20.361589574866137	29.02393393974582	28.3376878911038	22.276788594284238
31	21.535707373717823	30.45126120895426	24.344397135668665	23.668634281659248
32	23.297690471582477	28.43929423908135	26.085413844268114	22.17760144506806
33	22.48000129023934	27.32404361008967	24.23714599058125	25.958809109089735
34	21.63650732210825	27.076478936842786	27.485323527514353	23.80169021353461
35	20.001935359009096	25.304819043932646	27.032933359138124	27.66031223792013
36	25.041126378943297	28.57396297013096	25.054835171924395	21.330075479001355
37	19.921295400296753	30.238371717953683	29.749693568156893	20.09063931359267
38	19.174569382620476	27.9312624991936	25.424166182826912	27.470001935359008
39	22.670311592800466	24.710502548222696	28.280433520418036	24.338752338558802
40	22.336462163731372	22.92916586026708	28.36994387458874	26.364428101412813
41	20.04709373588801	26.537804012644344	24.72179214244242	28.693310109025223
42	22.852557899490357	23.907328559447777	28.15060318689117	25.089510354170695
43	24.313753951357977	23.69766466679569	28.297367911747628	23.691213470098702
44	20.71398619443907	25.039513579769046	28.35623508160764	25.890265144184244
45	19.74469389071673	23.567027933681697	27.603864266821493	29.08441390878008
46	23.544448745242242	25.34352622411457	26.828914263595898	24.283110767047287
47	18.879427133733305	24.82662408876847	31.881814076511194	24.412134700987036
48	20.52286949229082	23.329946455067414	28.98280756080253	27.16437649183924
49	21.573608154312627	20.356751177343398	32.78175601574092	25.28788465260306
50	21.338139474872587	21.275240307076963	30.26256370556738	27.124056512483065
51	19.589865169989032	21.597800141926328	26.667634346171216	32.14470034191342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	109.0
2	151.0
3	556.5
4	962.0
5	633.0
6	304.0
7	309.0
8	314.0
9	336.5
10	359.0
11	342.5
12	326.0
13	323.5
14	321.0
15	311.0
16	301.0
17	298.0
18	295.0
19	315.5
20	336.0
21	356.0
22	376.0
23	397.5
24	419.0
25	417.0
26	566.0
27	717.0
28	903.5
29	1090.0
30	1241.0
31	1392.0
32	1467.0
33	1542.0
34	1895.5
35	2249.0
36	2354.5
37	2460.0
38	3153.0
39	3846.0
40	4559.0
41	5272.0
42	6184.5
43	7097.0
44	8507.5
45	9918.0
46	13917.5
47	17917.0
48	16972.5
49	16028.0
50	15780.5
51	15533.0
52	12592.0
53	9651.0
54	8119.5
55	6588.0
56	5576.0
57	4564.0
58	4107.0
59	3650.0
60	3377.0
61	3104.0
62	2739.5
63	2375.0
64	2012.0
65	1649.0
66	1267.0
67	885.0
68	673.0
69	461.0
70	447.0
71	433.0
72	353.0
73	273.0
74	231.0
75	130.0
76	71.0
77	62.0
78	53.0
79	37.0
80	21.0
81	11.5
82	2.0
83	11.5
84	21.0
85	12.5
86	4.0
87	3.5
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	124008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.71163150764467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18944558669696	34.06554415844139
2	10.37225408505573	9.275208051093479
3	3.681059048443531	4.937584671956648
4	1.7909317173466075	3.20301916005419
5	1.1885438083901454	2.6570866395716406
6	0.8530822782527144	2.2885620282562416
7	0.705190635934062	2.207115669956777
8	0.5951736825018937	2.128894910005806
9	0.5789416729791148	2.3296884071995354
>10	3.962413880171699	28.231243145603507
>50	0.046892471954694655	1.3749112960454164
>100	0.023446235977347327	2.1635700922521126
>500	0.005410669840926307	1.3813624927424037
>1k	0.00721422645456841	3.7562092768208504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	1212	0.9773562995935746	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	1202	0.9692923037223405	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	1138	0.9176827301464421	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1106	0.891877943358493	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.4862589510354171	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	602	0.48545255144829363	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	508	0.40965099025869295	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT	281	0.2265982839816786	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	280	0.22579188439455516	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	273	0.22014708728469135	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	236	0.1903103025611251	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	231	0.18627830462550804	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	223	0.17982710792852075	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC	209	0.16853751370879297	No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	206	0.16611831494742274	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	184	0.1483775240307077	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	159	0.12821753435262243	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	159	0.12821753435262243	No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	132	0.1064447455002903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42819818076253147	0.0
2	0.0	0.0	0.0	2.285336429907748	0.0
3	0.0	0.0	0.0	2.9610992839171666	0.0
4	0.0	0.0	0.0	4.024740339332946	0.0
5	0.0	0.0	0.0	7.168085929940004	0.0
6	0.0	0.0	0.0	8.60912199212954	0.0
7	0.0	0.0	0.0	10.09128443326237	0.0
8	0.0	0.0	0.0	12.407264047480808	0.0
9	0.0	0.0	0.0	13.194310044513257	0.0
10	0.0	0.0	0.0	15.921553448164634	0.0
11	0.0	0.0	0.0	18.42381136700858	0.0
12	0.0	0.0	0.0	21.395393845558353	0.0
13	0.0	0.0	0.0	22.34371976001548	0.0
14	0.0	0.0	0.0	22.75901554738404	0.0
15	0.0	0.0	0.0	23.488000774143604	0.0
16	0.0	0.0	0.0	24.835494484226825	0.0
17	0.0	0.0	0.0	26.337816915037738	0.0
18	0.0	0.0	0.0	28.161892781110897	0.0
19	0.0	0.0	0.0	29.189245855106122	0.0
20	0.0	0.0	0.0	30.09644539061996	0.0
21	0.0	0.0	0.0	31.12702406296368	0.0
22	0.0	0.0	0.0	32.163247532417266	0.0
23	0.0	0.0	0.0	33.160763821688924	0.0
24	0.0	0.0	0.0	33.93087542739178	0.0
25	0.0	0.0	0.0	34.60825108057545	0.0
26	0.0	0.0	0.0	35.304980323850074	0.0
27	0.0	0.0	0.0	35.950906393135924	0.0
28	0.0	0.0	0.0	36.58312366944068	0.0
29	0.0	0.0	0.0	37.22340494161667	0.0
30	0.0	0.0	0.0	37.95642216631185	0.0
31	0.0	0.0	0.0	38.595897038900716	0.0
32	0.0	0.0	0.0	39.263595897038904	0.0
33	8.063995871234114E-4	0.0	0.0	39.881297980775436	0.0
34	8.063995871234114E-4	0.0	0.0	40.48529127153087	0.0
35	8.063995871234114E-4	0.0	0.0	41.16992452099865	0.0
36	8.063995871234114E-4	0.0	0.0	41.74891942455326	0.0
37	8.063995871234114E-4	0.0	0.0	42.34323592026321	0.0
38	8.063995871234114E-4	0.0	0.0	42.947229211018644	0.0
39	8.063995871234114E-4	0.0	0.0	43.539126507967225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	25	3.8706377E-5	45.000004	6
GGAATGC	30	2.1498145E-6	45.000004	8
CGTTGGG	25	3.8706377E-5	45.000004	3
TACGGGA	30	2.1498145E-6	45.000004	4
ACGGGTA	25	3.8706377E-5	45.000004	5
CCTCGAT	30	2.1498145E-6	45.000004	15
ACGGGCT	30	2.1498145E-6	45.000004	5
CAGGGAG	50	2.1827873E-11	45.000004	5
AGACGGG	50	2.1827873E-11	45.000004	3
CGGGCAT	30	2.1498145E-6	45.000004	6
TCGTCGG	30	2.1498145E-6	45.000004	2
GCCTCGA	30	2.1498145E-6	45.000004	14
CGGGATA	25	3.8706377E-5	45.000004	6
CTCGATC	30	2.1498145E-6	45.000004	16
CGATCAG	30	2.1498145E-6	45.000004	18
GAGTGGG	20	7.0089404E-4	45.0	3
ACTATGG	20	7.0089404E-4	45.0	2
AATTTGC	20	7.0089404E-4	45.0	11
CGTGCGG	20	7.0089404E-4	45.0	2
ACGTTAA	20	7.0089404E-4	45.0	21
>>END_MODULE