##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935452.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339806
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217656545205205	31.0	31.0	33.0	28.0	34.0
2	31.214010347080393	31.0	31.0	34.0	28.0	34.0
3	31.027389157342718	31.0	31.0	34.0	27.0	34.0
4	34.836774512515966	35.0	35.0	37.0	32.0	37.0
5	34.854761246122784	35.0	35.0	37.0	33.0	37.0
6	35.03945780827884	36.0	35.0	37.0	32.0	37.0
7	35.4852003790398	37.0	35.0	37.0	33.0	37.0
8	35.28430045378834	37.0	35.0	37.0	33.0	37.0
9	37.12123682336392	39.0	37.0	39.0	33.0	39.0
10	36.35718910201703	38.0	35.0	39.0	32.0	39.0
11	36.72102905775648	39.0	35.0	39.0	32.0	39.0
12	36.88025814729581	39.0	37.0	39.0	33.0	39.0
13	36.94237300106531	39.0	37.0	39.0	33.0	39.0
14	37.98173663796401	40.0	37.0	41.0	33.0	41.0
15	38.167395513910876	40.0	37.0	41.0	33.0	41.0
16	38.12124859478644	40.0	37.0	41.0	34.0	41.0
17	38.03490226776455	40.0	37.0	41.0	33.0	41.0
18	38.0060181397621	39.0	37.0	41.0	34.0	41.0
19	37.85679475936269	39.0	37.0	41.0	33.0	41.0
20	37.92488949577112	39.0	37.0	41.0	34.0	41.0
21	37.898580366444385	39.0	37.0	41.0	33.0	41.0
22	38.02554692971872	40.0	37.0	41.0	34.0	41.0
23	38.068756878925036	40.0	37.0	41.0	34.0	41.0
24	38.0599018263362	40.0	37.0	41.0	34.0	41.0
25	37.65872291837107	39.0	36.0	41.0	33.0	41.0
26	37.76042506606711	40.0	36.0	41.0	33.0	41.0
27	37.83044737291278	40.0	36.0	41.0	33.0	41.0
28	37.74881255775354	40.0	36.0	41.0	33.0	41.0
29	37.72976639612014	40.0	36.0	41.0	33.0	41.0
30	37.46370576152275	40.0	36.0	41.0	33.0	41.0
31	37.44784671253598	39.0	36.0	41.0	33.0	41.0
32	37.41677604280089	40.0	36.0	41.0	33.0	41.0
33	37.375167007056966	40.0	36.0	41.0	33.0	41.0
34	37.256558153770094	40.0	36.0	41.0	32.0	41.0
35	37.24471904557306	40.0	36.0	41.0	32.0	41.0
36	37.09130503875741	40.0	36.0	41.0	32.0	41.0
37	37.07281801969359	40.0	36.0	41.0	32.0	41.0
38	36.907582561814685	39.0	35.0	41.0	31.0	41.0
39	36.81768126519249	39.0	35.0	41.0	31.0	41.0
40	36.73777684914334	39.0	35.0	41.0	31.0	41.0
41	36.60025426272638	39.0	35.0	41.0	30.0	41.0
42	36.63947075684361	39.0	35.0	41.0	31.0	41.0
43	36.59472169414313	39.0	35.0	41.0	31.0	41.0
44	36.5938476660212	39.0	35.0	41.0	31.0	41.0
45	36.59757332124801	39.0	35.0	41.0	31.0	41.0
46	36.51539702065296	39.0	35.0	41.0	31.0	41.0
47	36.49392594598094	39.0	35.0	41.0	31.0	41.0
48	36.39681171021112	39.0	35.0	41.0	31.0	41.0
49	36.37419292184364	39.0	35.0	41.0	31.0	41.0
50	36.24859478643697	39.0	35.0	40.0	30.0	41.0
51	35.034516753677096	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	12.0
9	14.0
10	22.0
11	13.0
12	8.0
13	8.0
14	14.0
15	9.0
16	29.0
17	45.0
18	109.0
19	171.0
20	344.0
21	639.0
22	941.0
23	1247.0
24	1715.0
25	2384.0
26	3126.0
27	3395.0
28	3618.0
29	4068.0
30	4811.0
31	6357.0
32	8642.0
33	12453.0
34	21195.0
35	24378.0
36	27645.0
37	43506.0
38	79015.0
39	89864.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72436037032895	20.525535158296204	24.029004785083252	11.72109968629159
2	34.313402353107364	24.403336021141474	24.556364513869678	16.726897111881485
3	32.29430910578389	23.767090634067674	27.731411452416964	16.20718880773147
4	27.98567417879614	24.510455966051218	28.205799779874397	19.298070075278247
5	24.734701565010624	29.913538901608565	26.149037980494754	19.20272155288606
6	23.90746484758951	35.7315644809097	26.089592296781106	14.271378374719692
7	77.43447731941167	5.918965527389157	11.915328157831233	4.731228995367945
8	78.12987410463617	5.9342683766619775	10.62253167984085	5.313325838860997
9	71.72151168607971	7.249430557435713	12.715490603461976	8.313567153022607
10	37.25920083812528	23.927770551432285	22.094960065449108	16.71806854499332
11	28.646345267593865	25.070775677886793	26.269989346862623	20.012889707656722
12	24.418344584851358	21.33982331094801	31.91438644402983	22.327445660170802
13	24.27708751464071	22.75739686762447	33.89845970936358	19.06705590837125
14	20.504346597764606	26.944491857118475	31.25754106755031	21.29362047756661
15	19.891644055725916	25.412441216458802	34.87372206494294	19.822192662872347
16	21.752117384625343	25.149349923191465	31.281083912585416	21.81744877959777
17	22.08936863975327	25.20055561114283	29.2684649476466	23.4416108014573
18	22.970753900755135	24.48691312101611	31.233409651389323	21.308923326839434
19	22.47959129621019	27.185217447602454	28.777302343101653	21.5578889130857
20	23.267393748197502	26.199949382883176	29.805830385572946	20.72682648334638
21	22.432799891702913	28.305856871273612	29.785524681730163	19.475818555293316
22	21.019346332907602	24.23265039463694	29.985638864528585	24.762364407926878
23	20.37221238000506	27.25908312390011	29.6168990541662	22.75180544192863
24	21.58113747255787	25.429804064672197	29.93943603114718	23.049622431622748
25	20.37280095113094	27.97831703972267	28.20344549537089	23.44543651377551
26	19.07058733512651	25.973938070546136	31.16484111522457	23.790633479102784
27	21.408391847112764	25.361824099633317	30.21577017474677	23.014013878507146
28	20.050558259712894	25.23174988081435	31.819037921637637	22.898653937835117
29	21.21180909106961	24.2535446696056	32.25546341147596	22.27918282784883
30	21.82245163416773	25.365061240825646	31.641289441622572	21.17119768338405
31	22.913662501544998	25.1246299359046	28.374425407438363	23.587282155112035
32	22.321559948912025	26.751146242267648	29.828784659482178	21.098509149338152
33	22.40601990547548	25.320624120821883	29.09159932432035	23.181756649382297
34	21.128231991194976	24.641707327121946	30.691335644455954	23.538725037227124
35	20.042318263950605	25.945098085378127	31.335232456166167	22.6773511945051
36	23.787984909036332	26.63254916040329	29.068350764848176	20.5111151657122
37	21.553768915204557	26.071346591878896	30.52094430351436	21.853940189402188
38	21.918388727685798	28.65546812004497	27.706397179567166	21.71974597270207
39	22.570819820721237	25.38948694254957	28.450645368239524	23.58904786848967
40	21.36130615704255	25.873880979146925	29.764630406761505	23.00018245704902
41	20.63971795671648	26.105778002742742	28.24641118755996	25.008092852980816
42	21.838931625692307	25.766172463111303	29.73167042371235	22.663225487484034
43	23.038145294668134	24.069321907205875	30.199290183222192	22.693242614903795
44	21.671483140380097	24.378616033854613	29.94355602902833	24.00634479673696
45	21.25065478537754	23.95690482216323	29.685467590330955	25.106972802128276
46	22.568759821780663	25.48689546388233	28.98124223821828	22.963102476118728
47	20.15414677786737	24.688204446066283	31.82080363501527	23.33684514105107
48	20.878972119385768	24.66613302884587	30.405878648405267	24.049016203363095
49	22.121445766113606	22.94426820009064	31.61333231314338	23.32095372065237
50	20.74242361818214	22.970753900755135	31.158072547276976	25.12874993378575
51	20.56967799273703	22.701776896229024	29.09365932326092	27.634885787773023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	136.0
1	289.0
2	442.0
3	1809.0
4	3176.0
5	2096.0
6	1016.0
7	972.5
8	929.0
9	952.0
10	975.0
11	946.0
12	917.0
13	902.5
14	888.0
15	846.0
16	804.0
17	801.0
18	798.0
19	906.5
20	1015.0
21	1017.0
22	1019.0
23	1139.5
24	1260.0
25	1702.5
26	2190.0
27	2235.0
28	2901.0
29	3567.0
30	4049.5
31	4532.0
32	5275.5
33	6019.0
34	7089.0
35	8159.0
36	9012.0
37	9865.0
38	11032.0
39	12199.0
40	14040.0
41	15881.0
42	19109.5
43	22338.0
44	28696.5
45	35055.0
46	35991.5
47	36928.0
48	37196.5
49	37465.0
50	32581.0
51	27697.0
52	24840.5
53	21984.0
54	19230.5
55	16477.0
56	15887.0
57	15297.0
58	13873.0
59	12449.0
60	11548.0
61	10647.0
62	9442.0
63	8237.0
64	7158.5
65	6080.0
66	5106.0
67	4132.0
68	3633.5
69	3135.0
70	2378.5
71	1622.0
72	1365.0
73	1108.0
74	877.0
75	468.5
76	291.0
77	216.5
78	142.0
79	98.5
80	55.0
81	35.5
82	16.0
83	14.5
84	13.0
85	11.0
86	9.0
87	5.5
88	2.0
89	1.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.371200813292205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01423349705688	31.78443151358988
2	9.73151341605602	8.246718183379134
3	3.6960634231959215	4.698199365686957
4	1.9615147802472483	3.3244694660838756
5	1.4595412134522339	3.0921256925230387
6	1.1064109786320604	2.8127977054590105
7	0.874551445178545	2.5939056433650527
8	0.7528027547830856	2.551772535657095
9	0.6097871792616818	2.3253673523280955
>10	4.720082380997542	32.60392577797488
>50	0.04689931796475813	1.4144572269482951
>100	0.021699684431455254	1.8296889563942196
>500	0.0013999796407390488	0.38053022062794356
>1k	0.003499949101847622	2.341610359982552
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2051	0.6035796895875882	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	1934	0.5691482787237424	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	1348	0.3966969388415743	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	1286	0.3784512339393654	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1276	0.3755083783099769	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	659	0.19393418597670437	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	624	0.1836341912738445	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	488	0.14361135471416042	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	450	0.13242850332248401	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	345	0.10152851921390442	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9428556293885333E-4	0.0	0.0	0.19010847365849926	0.0
2	2.9428556293885333E-4	0.0	0.0	0.9732023566387881	0.0
3	2.9428556293885333E-4	0.0	0.0	1.4110992742918018	0.0
4	2.9428556293885333E-4	0.0	0.0	2.0338075254704155	0.0
5	2.9428556293885333E-4	0.0	0.0	3.802463758732924	0.0
6	2.9428556293885333E-4	0.0	0.0	4.920160326774689	0.0
7	2.9428556293885333E-4	0.0	0.0	5.870408409504247	0.0
8	2.9428556293885333E-4	0.0	0.0	7.48397615109798	0.0
9	2.9428556293885333E-4	0.0	0.0	8.14376438320689	0.0
10	2.9428556293885333E-4	0.0	0.0	9.8450292225564	0.0
11	2.9428556293885333E-4	0.0	0.0	11.435642690240902	0.0
12	2.9428556293885333E-4	0.0	0.0	13.123664679258164	0.0
13	5.885711258777067E-4	0.0	0.0	13.759027209643149	0.0
14	5.885711258777067E-4	0.0	0.0	14.032712783176283	0.0
15	5.885711258777067E-4	0.0	0.0	14.46972684414048	0.0
16	5.885711258777067E-4	0.0	0.0	15.340223539313609	0.0
17	5.885711258777067E-4	0.0	0.0	16.306657328004803	0.0
18	5.885711258777067E-4	0.0	0.0	17.38256534610925	0.0
19	5.885711258777067E-4	0.0	0.0	18.117984967893445	0.0
20	5.885711258777067E-4	0.0	0.0	18.76541320635892	0.0
21	5.885711258777067E-4	0.0	0.0	19.549978517153907	0.0
22	5.885711258777067E-4	0.0	0.0	20.371329523316245	0.0
23	5.885711258777067E-4	0.0	0.0	21.19621195623385	0.0
24	5.885711258777067E-4	0.0	0.0	21.82304020529361	0.0
25	5.885711258777067E-4	0.0	0.0	22.414259901237767	0.0
26	5.885711258777067E-4	0.0	0.0	22.986939606716774	0.0
27	5.885711258777067E-4	0.0	0.0	23.571096449150396	0.0
28	5.885711258777067E-4	0.0	0.0	24.16084471727986	0.0
29	5.885711258777067E-4	0.0	0.0	24.760304408986304	0.0
30	5.885711258777067E-4	0.0	0.0	25.46953261566894	0.0
31	5.885711258777067E-4	0.0	0.0	26.08282372883351	0.0
32	5.885711258777067E-4	0.0	0.0	26.687580560672856	0.0
33	5.885711258777067E-4	0.0	0.0	27.28851168019399	0.0
34	5.885711258777067E-4	0.0	0.0	27.88473423070811	0.0
35	5.885711258777067E-4	0.0	0.0	28.478308211155777	0.0
36	5.885711258777067E-4	0.0	0.0	29.04716220431658	0.0
37	5.885711258777067E-4	0.0	0.0	29.61778191085502	0.0
38	5.885711258777067E-4	0.0	0.0	30.21282731911738	0.0
39	5.885711258777067E-4	0.0	0.0	30.87732412023331	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAG	25	3.8849867E-5	45.000004	1
ACGACTC	25	3.8849867E-5	45.000004	40
GTACGAG	25	3.8849867E-5	45.000004	1
AACGTCT	40	6.7902874E-9	45.0	33
TAACGTC	40	6.7902874E-9	45.0	32
CACGACT	20	7.026267E-4	45.0	39
CGATGTT	20	7.026267E-4	45.0	13
CGTTTTT	965	0.0	42.901554	1
ATAACGT	45	1.9213985E-8	40.0	31
ACGGGCG	40	3.4486402E-7	39.375	5
TACGGGC	75	0.0	39.0	4
CGTCGGG	35	6.2353065E-6	38.57143	3
AATGGGT	65	9.094947E-12	38.07692	4
CGTAAGG	65	9.094947E-12	38.07692	2
ACGTTAG	30	1.13823015E-4	37.500004	1
TGTTCGG	30	1.13823015E-4	37.500004	2
AGCTGCG	30	1.13823015E-4	37.500004	1
CGATGTA	30	1.13823015E-4	37.500004	10
GTAAGCG	30	1.13823015E-4	37.500004	1
CTAATGG	30	1.13823015E-4	37.500004	2
>>END_MODULE