##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935450.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397735
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16279683709002	31.0	31.0	33.0	28.0	34.0
2	31.196364413491395	31.0	31.0	34.0	28.0	34.0
3	30.950366450023257	31.0	30.0	34.0	27.0	34.0
4	34.78485423711768	35.0	35.0	37.0	32.0	37.0
5	34.81593271902146	35.0	35.0	37.0	33.0	37.0
6	35.00378392648371	36.0	35.0	37.0	32.0	37.0
7	35.39219580876714	37.0	35.0	37.0	33.0	37.0
8	35.162935120117666	37.0	35.0	37.0	32.0	37.0
9	36.98315461299609	39.0	37.0	39.0	33.0	39.0
10	36.3753378505789	38.0	35.0	39.0	32.0	39.0
11	36.70549737890806	39.0	35.0	39.0	32.0	39.0
12	36.84214363835217	39.0	37.0	39.0	33.0	39.0
13	36.90053176109721	39.0	37.0	39.0	33.0	39.0
14	37.986428149395955	40.0	37.0	41.0	33.0	41.0
15	38.11718355186242	40.0	37.0	41.0	33.0	41.0
16	38.077767860510136	40.0	37.0	41.0	33.0	41.0
17	37.93408676631425	40.0	37.0	41.0	33.0	41.0
18	37.87295812538499	39.0	37.0	41.0	33.0	41.0
19	37.717108124756436	39.0	37.0	41.0	33.0	41.0
20	37.712225476762164	39.0	36.0	41.0	33.0	41.0
21	37.68019912755981	39.0	36.0	41.0	33.0	41.0
22	37.79850654330144	39.0	36.0	41.0	33.0	41.0
23	37.82614052069845	40.0	36.0	41.0	33.0	41.0
24	37.82818459527072	40.0	36.0	41.0	33.0	41.0
25	37.4614831483274	39.0	35.0	41.0	33.0	41.0
26	37.537739952480926	39.0	35.0	41.0	33.0	41.0
27	37.56163525965781	39.0	36.0	41.0	33.0	41.0
28	37.385153431304765	39.0	35.0	41.0	33.0	41.0
29	37.313570593485615	39.0	35.0	41.0	32.0	41.0
30	37.0272543276302	39.0	35.0	41.0	31.0	41.0
31	36.89323293147447	39.0	35.0	41.0	31.0	41.0
32	36.784695840195106	39.0	35.0	41.0	31.0	41.0
33	36.69048989905339	39.0	35.0	41.0	31.0	41.0
34	36.48989653915295	39.0	35.0	41.0	30.0	41.0
35	36.50777024903516	39.0	35.0	41.0	30.0	41.0
36	36.31150137654468	39.0	35.0	41.0	30.0	41.0
37	36.27192728826983	39.0	35.0	41.0	30.0	41.0
38	36.04971651979333	39.0	35.0	41.0	29.0	41.0
39	36.05995449231272	39.0	35.0	41.0	29.0	41.0
40	36.02161740857606	39.0	35.0	41.0	29.0	41.0
41	35.84127622663331	39.0	35.0	41.0	28.0	41.0
42	35.9006373590456	39.0	35.0	41.0	28.0	41.0
43	35.83708750801413	39.0	35.0	41.0	28.0	41.0
44	35.84022527562322	39.0	35.0	41.0	28.0	41.0
45	35.78830879857191	39.0	35.0	40.0	28.0	41.0
46	35.69712999861717	39.0	35.0	40.0	27.0	41.0
47	35.618467069782646	38.0	35.0	40.0	27.0	41.0
48	35.57259230392095	38.0	35.0	40.0	27.0	41.0
49	35.516409166907614	38.0	35.0	40.0	27.0	41.0
50	35.40820646913146	38.0	35.0	40.0	27.0	41.0
51	34.248564999308584	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	23.0
10	26.0
11	19.0
12	15.0
13	12.0
14	14.0
15	31.0
16	47.0
17	83.0
18	151.0
19	300.0
20	570.0
21	915.0
22	1580.0
23	2122.0
24	2939.0
25	4226.0
26	5209.0
27	5577.0
28	5610.0
29	5939.0
30	6879.0
31	8678.0
32	11034.0
33	15016.0
34	25035.0
35	28994.0
36	33101.0
37	49949.0
38	87972.0
39	95646.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95473116522307	19.65580097300967	22.440569726073896	14.948898135693364
2	35.62045080267012	24.925138597307253	22.659308333438094	16.795102266584536
3	29.96542924308899	25.007102719147174	29.48546142532088	15.542006612442957
4	26.27226671024677	23.7816636705344	31.04629967189209	18.89976994732674
5	25.552189271751292	28.057374885287945	27.33754887047909	19.052886972481677
6	22.707330257583568	35.02256527587464	27.889675286308723	14.38042918023307
7	74.29569939784028	5.489333350094912	15.344136171068676	4.87083108099614
8	74.08500634844809	7.0903993865262045	13.199240700466392	5.625353564559317
9	67.9869762530328	7.203540045507688	15.164116811444806	9.645366890014708
10	35.57519453907753	25.394798043923718	23.018089934252707	16.01191748274605
11	28.057374885287945	24.788364111782972	28.00382164003671	19.150439362892378
12	24.278728298993048	21.613134373389318	32.58476121035363	21.523376117264007
13	23.980036959281932	23.08471721120847	34.749770575885954	18.185475253623647
14	20.166442480546092	27.790865777464894	31.66379624624436	20.378895495744654
15	18.668711579317886	24.582196688750045	36.917294178284536	19.83179755364753
16	21.221667693313385	25.17203665757351	32.408261782342514	21.198033866770587
17	20.698455001445687	25.74528266307969	30.934918978716986	22.621343356757638
18	22.514488289941795	24.114548631626587	31.96525324650835	21.405709831923264
19	22.008120985078	26.67630457465398	29.470627427809976	21.844947012458043
20	24.02101902020189	25.774447810728248	30.372987039108956	19.831546129960902
21	22.37343960174488	28.361094698731566	30.640501841678503	18.62496385784505
22	20.92347920097552	24.429079663595108	30.984700868668835	23.662740266760533
23	20.166191056859468	27.109507586709743	30.633461978452992	22.0908393779778
24	20.939067469546305	25.08604975674758	30.790853206280566	23.184029567425547
25	20.42239179353087	28.0126214690686	28.964260122946182	22.60072661445435
26	20.464630972883953	25.859177593121046	30.544457993387557	23.13173344060744
27	23.876450400392223	25.164745370661368	28.742001583969223	22.216802644977182
28	20.116157743220988	26.278300878725787	31.40457842533345	22.200962952719774
29	23.496800633587693	24.714696971601693	30.40315788150402	21.3853445133066
30	24.432599595207865	25.04355915370787	30.288760104089405	20.235081146994858
31	23.32180974769633	25.008359837580297	29.565162733981172	22.104667680742203
32	23.87242762140621	27.29857819905213	28.788012118621698	20.04098206091996
33	22.24521352156587	25.5542006612443	30.00465133820257	22.195934478987265
34	21.36623631312306	25.01288546393956	32.48519743044992	21.13568079248746
35	21.792650885639937	25.3601015751694	29.72758243554125	23.119665103649414
36	22.00736671401813	27.83536776999761	29.85731705784002	20.299948458144243
37	21.023797251939104	28.38573422002087	30.239229637824177	20.351238890215846
38	19.456673413202257	28.079248746024366	29.71098847222397	22.75308936854941
39	21.56360390712409	26.104567111267553	30.23017838510566	22.101650596502697
40	22.21856261078356	23.45079009893522	32.1943002250242	22.136347065257016
41	19.549700177253698	26.199605264812	30.174110902988172	24.07658365494613
42	21.988509937521215	25.88255999597722	29.365029479427257	22.76390058707431
43	22.49336870026525	25.670861251838534	29.225489333350097	22.610280714546118
44	21.800445019925327	24.8989905338982	29.737136535633024	23.563427910543453
45	21.56083824657121	23.55789658943769	29.88899644235483	24.992268721636265
46	23.079185890102707	25.158962625868988	28.713087860007292	23.048763624021017
47	20.17448803851811	24.59376217833482	32.575458533948485	22.65629124919859
48	20.539806655184986	24.39991451594655	30.553006398732823	24.507272430135643
49	20.95013011175783	23.393465498384604	32.362754095063295	23.293650294794272
50	20.284360189573462	23.292644600047772	32.195557343457324	24.22743786692144
51	20.326599368926544	22.484820294919984	29.57421398669969	27.614366349453785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	311.5
2	477.0
3	3352.5
4	6228.0
5	4134.0
6	2040.0
7	1961.0
8	1882.0
9	1870.0
10	1858.0
11	1860.5
12	1863.0
13	1721.5
14	1580.0
15	1587.0
16	1594.0
17	1529.0
18	1464.0
19	1392.0
20	1320.0
21	1399.5
22	1479.0
23	1588.0
24	1697.0
25	2058.0
26	2789.0
27	3159.0
28	3703.5
29	4248.0
30	5128.5
31	6009.0
32	6583.0
33	7157.0
34	8196.5
35	9236.0
36	9947.0
37	10658.0
38	12304.5
39	13951.0
40	15968.5
41	17986.0
42	20590.5
43	23195.0
44	33077.5
45	42960.0
46	42353.5
47	41747.0
48	41451.5
49	41156.0
50	35919.0
51	30682.0
52	27450.0
53	24218.0
54	22377.5
55	20537.0
56	18852.0
57	17167.0
58	15888.5
59	14610.0
60	13879.5
61	13149.0
62	11484.5
63	9820.0
64	8407.5
65	6995.0
66	5716.5
67	4438.0
68	3687.0
69	2936.0
70	2620.5
71	2305.0
72	1865.5
73	1426.0
74	1114.5
75	670.5
76	538.0
77	399.5
78	261.0
79	211.5
80	162.0
81	145.5
82	129.0
83	79.0
84	29.0
85	18.5
86	8.0
87	8.0
88	8.0
89	4.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	397735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.51396812419338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.613510104953	32.146303583603455
2	9.081497772672371	7.721810136546527
3	3.544308195860023	4.52047816983331
4	2.1101650309242506	3.588459554460045
5	1.4671309931212326	3.118678013778613
6	1.1606764974858115	2.960697816996732
7	0.9656431742719802	2.8737326191240715
8	0.8309391399239894	2.826121608629852
9	0.6895791633282934	2.6385071911962306
>10	4.47034127238414	29.75980094105322
>50	0.03996378279296747	1.1832272532824941
>100	0.01670128236124014	1.210107255178753
>500	0.005964743700442907	1.7740801251530924
>1k	0.0035788462202657437	3.677995731163587
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3827	0.9621984487158536	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	3300	0.8296981658642061	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	2347	0.5900913925100884	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	2295	0.5770173608055614	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1632	0.4103234565728437	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	1103	0.27732032634794523	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	950	0.23885250229424113	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	938	0.23583541805473493	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	900	0.2262813179629653	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	810	0.20365318616666875	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	645	0.16216827787345844	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	631	0.15864834626070123	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	547	0.1375287565841578	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	539	0.13551736709115364	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATTCAT	520	0.13074031704526884	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	516	0.12973462229876676	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTC	462	0.11615774322098886	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16493393842633913	0.0
2	0.0	0.0	0.0	0.9727582435541252	0.0
3	0.0	0.0	0.0	1.5057764592002212	0.0
4	0.0	0.0	0.0	2.2318880661747142	0.0
5	0.0	0.0	0.0	4.358932455026588	0.0
6	0.0	0.0	0.0	5.752573949991829	0.0
7	0.0	0.0	0.0	6.8550668158447206	0.0
8	0.0	0.0	0.0	8.493092134209965	0.0
9	0.0	0.0	0.0	9.21618665694495	0.0
10	2.51423686625517E-4	0.0	0.0	11.059625127283242	0.0
11	2.51423686625517E-4	0.0	0.0	12.534225049341899	0.0
12	2.51423686625517E-4	0.0	0.0	14.318578953323193	0.0
13	2.51423686625517E-4	0.0	0.0	14.997925754585339	0.0
14	2.51423686625517E-4	0.0	0.0	15.30415980489522	0.0
15	2.51423686625517E-4	0.0	0.0	15.74767118810263	0.0
16	2.51423686625517E-4	0.0	0.0	16.462468729178976	0.0
17	2.51423686625517E-4	0.0	0.0	17.288144116057175	0.0
18	2.51423686625517E-4	0.0	0.0	18.229474398783108	0.0
19	5.02847373251034E-4	0.0	0.0	18.902535607879617	0.0
20	7.542710598765509E-4	0.0	0.0	19.46773605541378	0.0
21	7.542710598765509E-4	0.0	0.0	20.110877845801852	0.0
22	7.542710598765509E-4	0.0	0.0	20.777653462732722	0.0
23	7.542710598765509E-4	0.0	0.0	21.472337108879028	0.0
24	0.001005694746502068	0.0	0.0	22.002338240285617	0.0
25	0.001005694746502068	0.0	0.0	22.51700252680805	0.0
26	0.001257118433127585	0.0	0.0	23.011301494713816	0.0
27	0.001257118433127585	0.0	0.0	23.521691578563615	0.0
28	0.0015085421197531018	0.0	0.0	24.005682175317737	0.0
29	0.0015085421197531018	0.0	0.0	24.53492903566445	0.0
30	0.0015085421197531018	0.0	0.0	25.15192276264347	0.0
31	0.0015085421197531018	0.0	0.0	25.699272128427218	0.0
32	0.0015085421197531018	0.0	0.0	26.259192678542245	0.0
33	0.0015085421197531018	0.0	0.0	26.81257621280501	0.0
34	0.0015085421197531018	0.0	0.0	27.341823073151723	0.0
35	0.0015085421197531018	0.0	0.0	27.863024375526418	0.0
36	0.0015085421197531018	0.0	0.0	28.360591851358315	0.0
37	0.0015085421197531018	0.0	0.0	28.879530340553384	0.0
38	0.0017599658063786189	0.0	0.0	29.401988761361206	0.0
39	0.0017599658063786189	0.0	0.0	29.93752121387356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	25	3.8861897E-5	45.0	31
GCACGTG	35	1.209246E-7	45.0	12
CTAACGG	40	6.7957444E-9	45.0	2
GCGATAC	20	7.0277177E-4	45.0	9
TACTCAC	25	3.8861897E-5	45.0	30
CCAGTAT	20	7.0277177E-4	45.0	11
ATGTTAG	25	3.8861897E-5	45.0	1
GGCCTAT	40	6.7957444E-9	45.0	8
CGGAGAT	20	7.0277177E-4	45.0	22
GCATCCG	20	7.0277177E-4	45.0	9
ATCGAAT	25	3.8861897E-5	45.0	43
TACCGGA	20	7.0277177E-4	45.0	19
CGTTTTT	1960	0.0	43.27806	1
TAGGGTA	105	0.0	40.714287	5
CGTTTCT	100	0.0	40.5	1
ATGGGCG	90	0.0	40.0	5
CGTTCTG	90	0.0	40.0	1
GTAGGGT	125	0.0	39.600002	4
TTTAGCG	40	3.450623E-7	39.375	1
CGTGGCA	40	3.450623E-7	39.375	15
>>END_MODULE