##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935449.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299972
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2060725667729	31.0	31.0	33.0	28.0	34.0
2	31.238332244342807	31.0	31.0	34.0	28.0	34.0
3	31.040427106529943	31.0	31.0	34.0	27.0	34.0
4	34.858753483658475	35.0	35.0	37.0	32.0	37.0
5	34.85839678369981	35.0	35.0	37.0	33.0	37.0
6	35.056248583201096	36.0	35.0	37.0	32.0	37.0
7	35.52814596028963	37.0	35.0	37.0	33.0	37.0
8	35.28213966636886	37.0	35.0	37.0	32.0	37.0
9	37.13427586574747	39.0	37.0	39.0	34.0	39.0
10	36.303068286373396	37.0	35.0	39.0	32.0	39.0
11	36.711989785713335	39.0	35.0	39.0	32.0	39.0
12	36.87307815396104	39.0	37.0	39.0	33.0	39.0
13	36.92449628632006	39.0	37.0	39.0	33.0	39.0
14	38.001466803568334	40.0	37.0	41.0	33.0	41.0
15	38.16487538836958	40.0	37.0	41.0	33.0	41.0
16	38.11478071286653	40.0	37.0	41.0	34.0	41.0
17	38.05388169562492	40.0	37.0	41.0	33.0	41.0
18	38.052944941527876	39.0	37.0	41.0	34.0	41.0
19	37.9250563385916	39.0	37.0	41.0	34.0	41.0
20	37.95180550184684	39.0	37.0	41.0	34.0	41.0
21	38.00995092875335	40.0	37.0	41.0	34.0	41.0
22	38.12990212419826	40.0	37.0	41.0	34.0	41.0
23	38.12344485485312	40.0	37.0	41.0	34.0	41.0
24	38.13601269451816	40.0	37.0	41.0	34.0	41.0
25	37.785366634219194	40.0	36.0	41.0	33.0	41.0
26	37.84774578960703	40.0	36.0	41.0	33.0	41.0
27	37.89131985651994	40.0	37.0	41.0	33.0	41.0
28	37.82066326190444	40.0	36.0	41.0	33.0	41.0
29	37.836221380662195	40.0	36.0	41.0	33.0	41.0
30	37.59224527622578	40.0	36.0	41.0	33.0	41.0
31	37.592468630405506	40.0	36.0	41.0	33.0	41.0
32	37.54526755830544	40.0	36.0	41.0	33.0	41.0
33	37.54824116917579	40.0	36.0	41.0	33.0	41.0
34	37.42562305815209	40.0	36.0	41.0	33.0	41.0
35	37.47987812195805	40.0	36.0	41.0	33.0	41.0
36	37.344162121798036	40.0	36.0	41.0	33.0	41.0
37	37.321163308575464	40.0	36.0	41.0	33.0	41.0
38	37.18872761457736	40.0	36.0	41.0	32.0	41.0
39	37.095275559052176	40.0	36.0	41.0	32.0	41.0
40	37.01117770991959	39.0	36.0	41.0	31.0	41.0
41	36.85806341925247	39.0	35.0	41.0	31.0	41.0
42	36.940811142373285	39.0	35.0	41.0	31.0	41.0
43	36.89485685330631	39.0	35.0	41.0	31.0	41.0
44	36.926389796380995	39.0	35.0	41.0	31.0	41.0
45	36.93729414745376	39.0	35.0	41.0	31.0	41.0
46	36.84995599589295	39.0	35.0	41.0	31.0	41.0
47	36.76155774538957	39.0	35.0	41.0	31.0	41.0
48	36.73370181216914	39.0	35.0	41.0	31.0	41.0
49	36.72800794740842	39.0	35.0	41.0	31.0	41.0
50	36.5843978771352	39.0	35.0	41.0	31.0	41.0
51	35.40420439240996	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	18.0
11	11.0
12	10.0
13	10.0
14	3.0
15	14.0
16	16.0
17	41.0
18	73.0
19	132.0
20	341.0
21	578.0
22	797.0
23	1133.0
24	1452.0
25	1793.0
26	2287.0
27	2491.0
28	2730.0
29	3303.0
30	4087.0
31	5389.0
32	7387.0
33	10451.0
34	18245.0
35	21482.0
36	23835.0
37	37112.0
38	69962.0
39	84763.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11621084634566	20.331897643780085	23.876561812435828	10.675329697438428
2	33.639139653034285	25.06533943168029	24.385275959089515	16.91024495619591
3	31.639286333391116	25.720067206272585	26.294454149053912	16.346192311282387
4	27.97261077700585	24.69397143733415	28.634339204992465	18.699078580667532
5	25.83774485618658	29.670769271798704	25.71573346845706	18.775752403557668
6	23.337844865520783	36.94711506407265	25.78073953569	13.934300534716574
7	77.66958249436614	6.31592281946315	11.538743616070834	4.475751070099876
8	77.77859266864907	7.29868121024629	9.359540223754218	5.56318589735042
9	72.16140173082822	6.982651714159989	11.748096489005642	9.107850066006161
10	37.271145306895306	22.316416198845225	23.89089648367181	16.521542010587655
11	29.860120277892605	26.813835958022747	23.668875761737763	19.657168002346886
12	25.09067512967877	22.21607350019335	30.250156681290253	22.443094688837625
13	24.521955382502366	24.525622391423198	32.26067766324857	18.691744562825864
14	21.938714279999466	28.161961783099756	29.298401184110517	20.60092275279026
15	19.65150080674196	26.070766604883122	34.23086154707773	20.046871041297187
16	21.131305588521595	25.50404704439081	30.434173856226582	22.930473510861013
17	21.394996866374193	25.724734308535464	29.310068939767714	23.57019988532263
18	21.978384649233927	25.048671209312868	30.55851879508754	22.41442534636566
19	21.73769551824837	27.484231861640417	28.88002880268825	21.89804381742296
20	23.13115890816476	27.503233635139278	29.6817702985612	19.68383715813476
21	22.58510794340805	28.242969343805424	29.903791020495245	19.26813169229128
22	20.226554478417984	25.789407011321057	29.251063432587042	24.732975077673917
23	20.653927699918658	27.13653274305602	29.648433853826354	22.561105703198965
24	21.729694771512005	26.63248569866521	28.780686197378426	22.857133332444363
25	19.81451602149534	29.176056431933645	27.722920805941886	23.286506740629125
26	19.27646580347499	27.619577827263882	29.923126158441455	23.18083021081968
27	21.318323043484057	27.14886722760791	29.787113463923298	21.745696264984733
28	18.35037936874108	27.684917258944168	31.15557452028856	22.809128852026188
29	20.14988065552785	27.413558598802556	29.640099742642644	22.79646100302695
30	21.61135039270332	27.270211886442734	29.950795407571373	21.167642313282574
31	20.31389596362327	28.377315216086835	28.311309055511845	22.997479764778046
32	21.818036350059337	30.06680623524862	26.183110423639537	21.9320469910525
33	22.84046511007694	26.683157094662167	27.998946568346376	22.47743122691451
34	20.351232781726296	28.325977091195178	28.905697865134076	22.41709226194445
35	20.76927179870121	27.4785646660355	27.603242969343807	24.148920565919486
36	22.763457922739455	27.78626005093809	28.762684517221608	20.68759750910085
37	19.590161748429853	28.659674902990943	30.876548477857934	20.873614870721266
38	21.994719507154002	27.396223647540435	27.002186870774608	23.606869974530955
39	22.948475191017828	28.041283853159626	26.949848652540904	22.06039230328164
40	22.79646100302695	26.047431093568736	28.203965703465656	22.95214219993866
41	20.654594428813354	27.32188337578174	26.806168575733736	25.21735361967117
42	21.77569906524609	25.2843598735882	28.796687690851147	24.143253370314564
43	23.319509820916622	25.23168829090715	28.69467816996253	22.7541237182137
44	21.506673956235918	25.66606216580214	29.223394183457124	23.60386969450482
45	20.897283746483005	25.624058245436242	28.115624124918327	25.363033883162426
46	23.670209219527155	26.63648607203339	27.424559625565053	22.268745082874403
47	19.392476631152242	26.760497646447	31.21591348525862	22.631112237142133
48	20.412571840038403	25.97809128852026	28.87569506487272	24.733641806568613
49	21.548677876601815	23.986238715613457	30.963889963063217	23.50119344472151
50	21.416332191004493	23.763884629232063	29.86112037123465	24.958662808528796
51	20.291227181203578	24.339605029802783	27.461229714773378	27.90793807422026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	87.0
1	174.5
2	262.0
3	945.5
4	1629.0
5	1110.0
6	591.0
7	587.0
8	583.0
9	576.0
10	569.0
11	603.5
12	638.0
13	624.0
14	610.0
15	600.5
16	591.0
17	594.5
18	598.0
19	624.5
20	651.0
21	694.5
22	738.0
23	867.0
24	996.0
25	1298.0
26	1816.5
27	2033.0
28	2443.0
29	2853.0
30	3426.5
31	4000.0
32	4729.5
33	5459.0
34	6460.5
35	7462.0
36	8172.5
37	8883.0
38	10083.0
39	11283.0
40	13393.5
41	15504.0
42	17837.5
43	20171.0
44	27606.5
45	35042.0
46	34981.0
47	34920.0
48	34355.5
49	33791.0
50	30058.5
51	26326.0
52	23088.0
53	19850.0
54	17942.0
55	16034.0
56	14743.0
57	13452.0
58	11703.0
59	9954.0
60	8819.5
61	7685.0
62	6726.0
63	5767.0
64	4876.5
65	3986.0
66	3073.0
67	2160.0
68	1614.0
69	1068.0
70	1005.0
71	942.0
72	768.0
73	594.0
74	429.5
75	221.5
76	178.0
77	132.5
78	87.0
79	57.0
80	27.0
81	29.5
82	32.0
83	21.5
84	11.0
85	8.5
86	6.0
87	3.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	299972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.88981240518512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.62246089750235	24.628472483935106
2	12.084021180602328	8.673865065442023
3	4.431880599405962	4.77178089944578
4	2.4481718242821233	3.5145771003658095
5	1.69423957503863	3.0402970258788486
6	1.2970363503040845	2.793023477707174
7	1.062389705529866	2.6690277062866716
8	0.9401336242746935	2.699297552882043
9	0.8983906209130835	2.901876376603351
>10	6.44077831823096	38.13595152171788
>50	0.05366486927727515	1.3289552428660294
>100	0.01987587751010191	1.5279775256140822
>500	0.0029813816265152867	0.7828923129605301
>1k	0.003975175502020382	2.5320057082946703
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	2133	0.7110663661941782	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGC	1886	0.6287253476991186	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG	1695	0.5650527382555705	No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1385	0.46170975957756055	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.31169575827077195	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	644	0.21468670409238197	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	616	0.2053524995666262	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT	413	0.13767951675489715	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTAGACGT	396	0.132012321149974	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	368	0.12267811662421826	No Hit
GAACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT	366	0.12201138772952143	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTAGACGTCGTA	332	0.11067699651967518	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTC	319	0.10634325870414571	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3336444734841916E-4	0.0	0.0	0.21768698411851772	0.0
2	3.3336444734841916E-4	0.0	0.0	1.1794434147187072	0.0
3	3.3336444734841916E-4	0.0	0.0	1.5464776712493167	0.0
4	3.3336444734841916E-4	0.0	0.0	2.1722027389222993	0.0
5	3.3336444734841916E-4	0.0	0.0	4.180056805301828	0.0
6	3.3336444734841916E-4	0.0	0.0	5.041137172802795	0.0
7	3.3336444734841916E-4	0.0	0.0	5.962889869721174	0.0
8	3.3336444734841916E-4	0.0	0.0	7.468030349499287	0.0
9	3.3336444734841916E-4	0.0	0.0	7.984078513994639	0.0
10	3.3336444734841916E-4	0.0	0.0	9.802581574280266	0.0
11	3.3336444734841916E-4	0.0	0.0	11.504407077993946	0.0
12	3.3336444734841916E-4	0.0	0.0	13.471924046244316	0.0
13	3.3336444734841916E-4	0.0	0.0	14.087981544944196	0.0
14	3.3336444734841916E-4	0.0	0.0	14.312002453562332	0.0
15	3.3336444734841916E-4	0.0	0.0	14.802714920059206	0.0
16	3.3336444734841916E-4	0.0	0.0	15.702132199005241	0.0
17	3.3336444734841916E-4	0.0	0.0	16.76056431933647	0.0
18	3.3336444734841916E-4	0.0	0.0	17.953675676396465	0.0
19	3.3336444734841916E-4	0.0	0.0	18.743749416612218	0.0
20	3.3336444734841916E-4	0.0	0.0	19.39314336004694	0.0
21	3.3336444734841916E-4	0.0	0.0	20.159548224500952	0.0
22	3.3336444734841916E-4	0.0	0.0	20.989959062845866	0.0
23	3.3336444734841916E-4	0.0	0.0	21.847372421425998	0.0
24	3.3336444734841916E-4	0.0	0.0	22.514434680570186	0.0
25	3.3336444734841916E-4	0.0	0.0	23.13382582374355	0.0
26	3.3336444734841916E-4	0.0	0.0	23.68854426413132	0.0
27	3.3336444734841916E-4	0.0	0.0	24.285599989332336	0.0
28	3.3336444734841916E-4	0.0	0.0	24.846652354219728	0.0
29	3.3336444734841916E-4	0.0	0.0	25.44437480831544	0.0
30	3.3336444734841916E-4	0.0	0.0	26.093102022855465	0.0
31	3.3336444734841916E-4	0.0	0.0	26.68715746803035	0.0
32	3.3336444734841916E-4	0.0	0.0	27.263544597495766	0.0
33	3.3336444734841916E-4	0.0	0.0	27.855933220433908	0.0
34	3.3336444734841916E-4	0.0	0.0	28.449321936714092	0.0
35	3.3336444734841916E-4	0.0	0.0	29.023042150600723	0.0
36	3.3336444734841916E-4	0.0	0.0	29.59142853332978	0.0
37	3.3336444734841916E-4	0.0	0.0	30.16214846719027	0.0
38	3.3336444734841916E-4	0.0	0.0	30.736868774418944	0.0
39	3.3336444734841916E-4	0.0	0.0	31.37059458882829	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAT	20	7.024941E-4	45.000004	31
GCGACAG	30	2.160119E-6	45.000004	1
CGCATGG	25	3.88389E-5	45.000004	2
GGCCGTT	20	7.024941E-4	45.000004	13
CGTCTGG	35	1.2079181E-7	45.000004	2
AGGCGAC	25	3.88389E-5	45.000004	9
TAGCACG	25	3.88389E-5	45.000004	1
GCTAGCG	35	1.2079181E-7	45.000004	1
TTACGAG	40	6.7866495E-9	45.000004	1
GGTCTCG	20	7.024941E-4	45.000004	8
CTGGGCG	20	7.024941E-4	45.000004	5
CTAGGCG	20	7.024941E-4	45.000004	1
CCGTTGG	30	2.160119E-6	45.000004	2
TAGTACG	20	7.024941E-4	45.000004	1
ACGGTTA	30	2.160119E-6	45.000004	34
CTAGCGG	50	2.1827873E-11	45.000004	2
GGCGTAG	55	1.8189894E-12	45.0	1
CGTTTTT	595	0.0	43.865547	1
CGTATGG	60	3.6379788E-12	41.250004	2
TAGGGTA	210	0.0	40.714287	5
>>END_MODULE