SRR2935442.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935442.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401593
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	13875	3.454990500332426	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9813	2.4435186868297007	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	6845	1.704461980163997	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	6785	1.6895214807031	No Hit
GAACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCT	6124	1.5249269783088848	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4985	1.2413064968761907	No Hit
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2748	0.6842748753090816	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	2441	0.6078293197341587	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT	1870	0.4656455665312891	No Hit
CGTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCT	1791	0.4459739089077748	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCT	1392	0.34661958749280986	No Hit
GACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	1318	0.32819297149103693	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTC	1173	0.29208676446053594	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT	1116	0.27789328997268375	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGGCTTATCGTA	955	0.2378029497526102	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGGCTTAT	937	0.23332079991434115	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCT	851	0.2119060840203888	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	717	0.1785389685577189	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	579	0.14417581979765584	No Hit
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	550	0.13695457839155564	TruSeq Adapter, Index 20 (95% over 22bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	508	0.12649622876892774	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	488	0.12151606228196209	No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	452	0.11255176260542389	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC	417	0.10383647125323399	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	35	1.2092823E-7	45.0	16
CGGCCTA	20	7.0278E-4	45.0	37
ATGGACG	20	7.0278E-4	45.0	1
CACGACG	70	0.0	45.0	26
GCGGGTC	35	1.2092823E-7	45.0	5
GCGATTC	20	7.0278E-4	45.0	9
ACGCTTA	20	7.0278E-4	45.0	30
AGACACG	20	7.0278E-4	45.0	1
CGGTCTA	70	0.0	45.0	31
CCTATCG	20	7.0278E-4	45.0	40
CCAGTCG	35	1.2092823E-7	45.0	28
CGTTTTC	110	0.0	45.0	1
TATCGAA	25	3.886257E-5	45.0	42
ACAAGTC	20	7.0278E-4	45.0	18
GTACGAG	20	7.0278E-4	45.0	1
CCATACG	30	2.1619617E-6	44.999996	2
ATCGGTA	30	2.1619617E-6	44.999996	27
AATCGGT	30	2.1619617E-6	44.999996	26
CGTTTTT	3865	0.0	44.476067	1
CGACGGT	75	0.0	42.0	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content