SRR2935438.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935438.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268720
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	22702	8.448198868710925	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	15845	5.896472164334623	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15435	5.743896993152724	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15422	5.739059243822566	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	12755	4.746576362012504	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	6566	2.4434355462935398	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	5506	2.0489729086037514	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	4716	1.754986603155701	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	4559	1.6965614766299493	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	3934	1.4639773742185174	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	3591	1.3363352188151236	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	3259	1.212786543614171	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	2480	0.922893718368562	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT	2205	0.820556713307532	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	1941	0.7223131884489431	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	1632	0.6073236082167313	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA	1613	0.6002530515034237	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGT	950	0.3535278356653766	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGTGCTGATCG	828	0.3081274188746651	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	819	0.30477820779994047	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	767	0.2854272104793093	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	726	0.2701696933611194	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	711	0.26458767490324503	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	689	0.2564007144983626	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC	684	0.2545400416790711	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTAT	671	0.24970229234891334	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATG	616	0.22923489133670735	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	544	0.20244120273891036	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGT	533	0.19834772253646918	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	436	0.16225066984221495	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTGCTGA	371	0.13806192319142602	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	368	0.13694551949985115	No Hit
GAATGATACGGCGACCACTGAATCTGTCTCTTATACACATCTGACGCAGTG	350	0.1302470973504019	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	339	0.1261536171479607	No Hit
GACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	291	0.10829115808276273	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGT	282	0.1049419470080381	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	20	7.0236303E-4	45.0	5
CGGCGGA	20	7.0236303E-4	45.0	31
GATCGGG	20	7.0236303E-4	45.0	3
GCGCCGA	50	2.1827873E-11	45.0	37
AGGGTAA	25	3.882803E-5	45.0	6
TCGATGG	20	7.0236303E-4	45.0	2
ACGGGAG	20	7.0236303E-4	45.0	5
CGGCTAT	25	3.882803E-5	45.0	31
CAATGGG	20	7.0236303E-4	45.0	3
CACCGTA	25	3.882803E-5	45.0	40
CGATCGA	20	7.0236303E-4	45.0	41
CTAGGGT	25	3.882803E-5	45.0	4
GAGGGCG	20	7.0236303E-4	45.0	5
CACCCGC	20	7.0236303E-4	45.0	38
CCGGTGA	55	1.8189894E-12	44.999996	18
CGTTTTT	3745	0.0	44.579437	1
CGTTCTG	275	0.0	44.18182	1
CGTCTGT	480	0.0	44.062504	1
ATGAATG	825	0.0	43.909092	21
GATGAAT	800	0.0	43.875	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content