##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935438.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268720
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334236379874962	31.0	31.0	34.0	30.0	34.0
2	31.42394685918428	31.0	31.0	34.0	30.0	34.0
3	31.463352188151237	31.0	31.0	34.0	30.0	34.0
4	35.30692914557904	37.0	35.0	37.0	33.0	37.0
5	35.226328520392975	35.0	35.0	37.0	33.0	37.0
6	35.02465391485561	35.0	35.0	37.0	32.0	37.0
7	35.19707502232807	36.0	35.0	37.0	32.0	37.0
8	34.48874292944328	35.0	35.0	37.0	30.0	37.0
9	36.31433834474546	38.0	35.0	39.0	32.0	39.0
10	36.393502530515036	38.0	35.0	39.0	32.0	39.0
11	36.44048824054778	38.0	35.0	39.0	32.0	39.0
12	36.44166046442393	38.0	35.0	39.0	32.0	39.0
13	36.12880693658827	38.0	35.0	39.0	30.0	39.0
14	37.28099136647812	39.0	36.0	41.0	32.0	41.0
15	36.8828222685323	39.0	36.0	41.0	30.0	41.0
16	37.28501041976779	39.0	36.0	41.0	32.0	41.0
17	36.68891038999702	39.0	35.0	40.0	30.0	41.0
18	36.38070110151831	38.0	35.0	40.0	30.0	41.0
19	35.77931303959512	37.0	35.0	40.0	29.0	41.0
20	35.249039892825245	36.0	34.0	40.0	27.0	41.0
21	35.76643718368562	37.0	34.0	40.0	30.0	41.0
22	35.841682792497764	37.0	35.0	40.0	30.0	41.0
23	35.691187853527836	37.0	35.0	40.0	30.0	41.0
24	35.579324203632034	36.0	35.0	40.0	30.0	41.0
25	35.69358067877344	37.0	35.0	40.0	30.0	41.0
26	35.434619678475734	36.0	34.0	40.0	29.0	41.0
27	35.29805001488538	37.0	35.0	40.0	29.0	41.0
28	35.04788999702292	36.0	34.0	40.0	27.0	41.0
29	34.85380321524263	36.0	34.0	40.0	27.0	41.0
30	34.6767267043763	36.0	34.0	40.0	25.0	41.0
31	34.522435992855016	36.0	33.0	40.0	25.0	41.0
32	34.35376972313188	36.0	34.0	40.0	24.0	41.0
33	33.988259154510274	36.0	33.0	40.0	21.0	41.0
34	33.700517267043764	36.0	33.0	40.0	20.0	41.0
35	33.70145876749032	37.0	33.0	40.0	18.0	41.0
36	33.44992557308723	36.0	33.0	40.0	18.0	41.0
37	33.46110077403989	37.0	33.0	40.0	16.0	41.0
38	33.30874144090503	36.0	33.0	40.0	16.0	41.0
39	33.13130395951176	36.0	33.0	40.0	15.0	41.0
40	32.93112161357547	35.0	33.0	40.0	15.0	41.0
41	32.636037511164034	35.0	32.0	40.0	13.0	41.0
42	32.824036171479605	36.0	33.0	40.0	12.0	41.0
43	32.81089237868413	36.0	33.0	40.0	12.0	41.0
44	32.863266597201545	36.0	33.0	40.0	12.0	41.0
45	32.84592884787139	36.0	32.0	40.0	13.0	41.0
46	32.62612012503721	36.0	31.0	40.0	12.0	41.0
47	32.23117371241441	35.0	31.0	39.0	10.0	41.0
48	32.20385531408157	35.0	31.0	39.0	10.0	41.0
49	32.199914409050315	35.0	31.0	40.0	10.0	41.0
50	31.94950133968443	35.0	31.0	40.0	10.0	41.0
51	30.510282077999406	35.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	11.0
11	12.0
12	18.0
13	25.0
14	31.0
15	47.0
16	61.0
17	123.0
18	259.0
19	569.0
20	1128.0
21	1912.0
22	2835.0
23	3725.0
24	4402.0
25	5384.0
26	6480.0
27	6961.0
28	6647.0
29	6919.0
30	7720.0
31	9581.0
32	12697.0
33	16516.0
34	21774.0
35	31704.0
36	26691.0
37	24687.0
38	36252.0
39	33541.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.15212860970527	16.89453706460256	7.94656147662995	24.006772849062223
2	29.026868115510567	56.891932122655554	7.263694551949985	6.817505209883894
3	8.755209883894016	57.021062816314384	27.12116701399226	7.102560285799345
4	9.515480797856505	18.92192616850253	61.59236379874963	9.970229234891336
5	35.846233998213755	19.848169097945817	28.614170884191726	15.691426019648706
6	11.514587674903245	45.521732658529324	33.683015778505506	9.280663888061923
7	31.0323012801429	13.400565644537066	49.22409943435546	6.343033640964572
8	24.65800833581423	34.863054480500146	30.52247692765704	9.956460256028581
9	22.089163441500446	13.793167609407561	27.56884489431378	36.54882405477821
10	27.360821673116998	19.212191128311996	34.81169991068771	18.615287287883298
11	23.321673116999104	20.8012057159869	37.726629949389704	18.150491217624293
12	13.234965763620124	19.857472462042274	40.525454004167905	26.382107770169693
13	17.352634712712117	23.993376004763324	50.034980649002684	8.619008633521881
14	11.138359630842512	41.27865436141709	31.684653170586486	15.898332837153914
15	5.864468591842811	28.33618636498958	47.411059839237865	18.38828520392974
16	6.582688300089313	33.549047335516526	27.07986007740399	32.78840428699017
17	7.091768383447454	37.3876153617148	35.11498958023221	20.405626674605536
18	6.931006251860674	33.799121762429294	31.655998809169393	27.613873176540636
19	6.968963977374218	34.95459958320929	29.84333134861566	28.233105090800837
20	13.061178922298303	32.29160464423936	40.43874665078893	14.208469782673413
21	9.727597499255731	42.1520541827925	34.51622506698423	13.60412325096755
22	8.839312295325989	25.70779994045847	39.85896100029771	25.593926763917835
23	14.524412027389102	35.23704971717773	36.60315570110152	13.635382554331645
24	19.447752307234296	30.110151830902055	27.16768383447455	23.274412027389104
25	11.884861565942245	47.941351592735934	23.853081274188746	16.320705567133075
26	12.954376302470974	26.187109258707945	35.26756475141411	25.590949687406965
27	23.724694849657634	31.097796963381956	23.156445370646026	22.02106281631438
28	12.160613277761238	31.95184578743674	34.4075617743376	21.479979160464424
29	22.555820184578742	30.94633819589163	28.561699910687704	17.93614170884192
30	22.013620125037214	34.92855016373921	31.03788329860077	12.019946412622804
31	20.63783864245311	22.549121762429294	32.83343256921703	23.979607025900567
32	32.73258410241143	28.278877642155404	26.15064007144984	12.837898183983329
33	19.00937779100923	23.33879130693659	26.740845489729086	30.910985412325097
34	20.14178326883001	29.791976778803214	34.17088419172373	15.89535576064305
35	29.101667162846084	26.341172968145283	25.93629056266746	18.620869306341174
36	14.074501339684431	40.25267936885978	31.862905626674603	13.809913664781185
37	25.103825543316464	25.452515629651685	34.28215242631736	15.161506400714497
38	16.091842810360227	25.369901756475144	29.5690681750521	28.969187258112534
39	25.0293986305448	27.35672819291456	33.752232807383145	13.861640369157488
40	20.33045549270616	26.31251860672819	33.51853230128014	19.8384935992855
41	14.027612384638285	37.268532301280146	32.48474248288181	16.219112831199762
42	21.51570407859482	24.57948794284013	39.13701994641262	14.767788032152426
43	21.67423340279845	29.1764662101816	25.13545698124442	24.01384340577553
44	22.082837153914856	22.670437630247097	30.32077999404585	24.9259452217922
45	16.599806490026793	19.520318547186662	32.71360523965466	31.166269723131883
46	28.656594224471572	27.315793390890146	26.897514141113426	17.13009824352486
47	12.494417981542126	21.596085144388212	46.309913664781185	19.59958320928848
48	17.6659720154808	25.325989877939865	32.469857100327474	24.53818100625186
49	14.320482286394762	19.145579041381364	44.828445966061324	21.70549270616255
50	16.422670437630245	19.822119678475737	38.93569514736529	24.819514736528728
51	18.59333134861566	19.618934206609108	32.751562965168205	29.036171479607027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17512.0
1	11350.0
2	5188.0
3	12468.5
4	19749.0
5	11907.5
6	4066.0
7	3679.0
8	3292.0
9	3238.5
10	3185.0
11	3054.0
12	2923.0
13	2658.0
14	2393.0
15	2099.5
16	1806.0
17	1619.5
18	1433.0
19	1255.0
20	1077.0
21	1008.0
22	939.0
23	907.0
24	875.0
25	858.5
26	861.0
27	880.0
28	977.5
29	1075.0
30	1248.0
31	1421.0
32	1613.0
33	1805.0
34	2034.5
35	2264.0
36	2738.5
37	3213.0
38	3301.5
39	3390.0
40	3967.0
41	4544.0
42	5378.0
43	6212.0
44	8631.5
45	11051.0
46	49408.0
47	87765.0
48	56229.5
49	24694.0
50	23372.5
51	22051.0
52	15170.0
53	8289.0
54	7436.5
55	6584.0
56	6134.5
57	5685.0
58	4809.5
59	3934.0
60	3266.5
61	2599.0
62	2240.0
63	1881.0
64	1593.0
65	1305.0
66	1107.5
67	910.0
68	784.5
69	659.0
70	559.5
71	460.0
72	392.5
73	325.0
74	290.0
75	179.5
76	104.0
77	78.0
78	52.0
79	37.5
80	23.0
81	12.5
82	2.0
83	3.0
84	4.0
85	3.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	268720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.505730872283415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37236962488564	24.82323608216731
2	8.964928331808478	5.469633819589164
3	3.7548032936871	3.4362905626674607
4	1.9444952729490699	2.3727299791604644
5	1.2235437633424824	1.8662548377493302
6	0.7453491918267764	1.3642453111044954
7	0.437938395852394	0.9351741589758856
8	0.32204940530649584	0.7859481988687109
9	0.20860018298261668	0.5727150937779101
>10	0.8539188777066178	4.497618338791307
>50	0.0878316559926807	1.8926763917832687
>100	0.048795364440378165	2.6015927359333135
>500	0.014638609332113448	3.17728490622209
>1k	0.012198841110094541	11.137987496278654
>5k	0.002439768222018908	4.492408454897292
>10k+	0.006099420555047271	30.574203632033342
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	22702	8.448198868710925	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	15845	5.896472164334623	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15435	5.743896993152724	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15422	5.739059243822566	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	12755	4.746576362012504	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	6566	2.4434355462935398	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	5506	2.0489729086037514	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	4716	1.754986603155701	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	4559	1.6965614766299493	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	3934	1.4639773742185174	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	3591	1.3363352188151236	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	3259	1.212786543614171	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	2480	0.922893718368562	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT	2205	0.820556713307532	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	1941	0.7223131884489431	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	1632	0.6073236082167313	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA	1613	0.6002530515034237	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGT	950	0.3535278356653766	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGTGCTGATCG	828	0.3081274188746651	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	819	0.30477820779994047	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	767	0.2854272104793093	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	726	0.2701696933611194	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	711	0.26458767490324503	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	689	0.2564007144983626	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC	684	0.2545400416790711	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTAT	671	0.24970229234891334	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATG	616	0.22923489133670735	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	544	0.20244120273891036	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGT	533	0.19834772253646918	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	436	0.16225066984221495	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTGCTGA	371	0.13806192319142602	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	368	0.13694551949985115	No Hit
GAATGATACGGCGACCACTGAATCTGTCTCTTATACACATCTGACGCAGTG	350	0.1302470973504019	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	339	0.1261536171479607	No Hit
GACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	291	0.10829115808276273	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGT	282	0.1049419470080381	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7213456385829115E-4	0.0	0.0	0.17936885977969633	0.0
2	3.7213456385829115E-4	0.0	0.0	2.1327031854718665	0.0
3	3.7213456385829115E-4	0.0	0.0	4.540785948198868	0.0
4	3.7213456385829115E-4	0.0	0.0	9.198050014885382	0.0
5	3.7213456385829115E-4	0.0	0.0	19.527016969336113	0.0
6	3.7213456385829115E-4	0.0	0.0	23.034385233700505	0.0
7	3.7213456385829115E-4	0.0	0.0	25.507963679666567	0.0
8	3.7213456385829115E-4	0.0	0.0	27.523072342959214	0.0
9	3.7213456385829115E-4	0.0	0.0	28.100625186067283	0.0
10	3.7213456385829115E-4	0.0	0.0	34.80314081571896	0.0
11	3.7213456385829115E-4	0.0	0.0	35.49084548972909	0.0
12	3.7213456385829115E-4	0.0	0.0	41.04197677880322	0.0
13	3.7213456385829115E-4	0.0	0.0	41.60129502828223	0.0
14	3.7213456385829115E-4	0.0	0.0	42.0422744864543	0.0
15	3.7213456385829115E-4	0.0	0.0	42.84980649002679	0.0
16	3.7213456385829115E-4	0.0	0.0	43.407264066686515	0.0
17	3.7213456385829115E-4	0.0	0.0	43.91708841917237	0.0
18	3.7213456385829115E-4	0.0	0.0	44.24121762429294	0.0
19	3.7213456385829115E-4	0.0	0.0	45.38292646621018	0.0
20	3.7213456385829115E-4	0.0	0.0	45.69626376897886	0.0
21	7.442691277165823E-4	0.0	0.0	45.83841917237273	0.0
22	7.442691277165823E-4	0.0	0.0	46.06207204525156	0.0
23	7.442691277165823E-4	0.0	0.0	46.24292944328669	0.0
24	0.0011164036915748735	0.0	0.0	46.653766001786245	0.0
25	0.0011164036915748735	0.0	0.0	46.814900267936885	0.0
26	0.0011164036915748735	0.0	0.0	47.00729383745162	0.0
27	0.0011164036915748735	0.0	0.0	47.33179517713605	0.0
28	0.0011164036915748735	0.0	0.0	47.45646025602858	0.0
29	0.0011164036915748735	0.0	0.0	47.715838047037806	0.0
30	0.0011164036915748735	0.0	0.0	47.843108067877345	0.0
31	0.0014885382554331646	0.0	0.0	48.023593331348614	0.0
32	0.0014885382554331646	0.0	0.0	48.1575617743376	0.0
33	0.0014885382554331646	0.0	0.0	48.27143495087824	0.0
34	0.0018606728192914557	0.0	0.0	48.42103304554927	0.0
35	0.0018606728192914557	0.0	0.0	48.55090800833582	0.0
36	0.0018606728192914557	0.0	0.0	48.66031557011015	0.0
37	0.0018606728192914557	0.0	0.0	48.777537957725514	0.0
38	0.0018606728192914557	0.0	0.0	48.919321226555525	0.0
39	0.0018606728192914557	0.0	0.0	49.06743078297112	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	20	7.0236303E-4	45.0	5
CGGCGGA	20	7.0236303E-4	45.0	31
GATCGGG	20	7.0236303E-4	45.0	3
GCGCCGA	50	2.1827873E-11	45.0	37
AGGGTAA	25	3.882803E-5	45.0	6
TCGATGG	20	7.0236303E-4	45.0	2
ACGGGAG	20	7.0236303E-4	45.0	5
CGGCTAT	25	3.882803E-5	45.0	31
CAATGGG	20	7.0236303E-4	45.0	3
CACCGTA	25	3.882803E-5	45.0	40
CGATCGA	20	7.0236303E-4	45.0	41
CTAGGGT	25	3.882803E-5	45.0	4
GAGGGCG	20	7.0236303E-4	45.0	5
CACCCGC	20	7.0236303E-4	45.0	38
CCGGTGA	55	1.8189894E-12	44.999996	18
CGTTTTT	3745	0.0	44.579437	1
CGTTCTG	275	0.0	44.18182	1
CGTCTGT	480	0.0	44.062504	1
ATGAATG	825	0.0	43.909092	21
GATGAAT	800	0.0	43.875	20
>>END_MODULE