##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935436.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466787
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.836107260913437	33.0	31.0	34.0	30.0	34.0
2	31.90423040915878	33.0	31.0	34.0	30.0	34.0
3	31.969960603015938	33.0	31.0	34.0	30.0	34.0
4	35.69067690402689	37.0	35.0	37.0	33.0	37.0
5	35.70310441379044	37.0	35.0	37.0	35.0	37.0
6	35.646673964784796	37.0	35.0	37.0	33.0	37.0
7	35.914809109936655	37.0	35.0	37.0	35.0	37.0
8	35.745282109398936	37.0	35.0	37.0	35.0	37.0
9	37.558631667120125	39.0	37.0	39.0	35.0	39.0
10	36.97404169353474	39.0	37.0	39.0	32.0	39.0
11	36.92864411391063	39.0	37.0	39.0	33.0	39.0
12	36.95068628732162	39.0	37.0	39.0	33.0	39.0
13	36.9032877950757	39.0	37.0	39.0	33.0	39.0
14	37.92736515798426	40.0	37.0	41.0	33.0	41.0
15	38.00988030943449	40.0	37.0	41.0	33.0	41.0
16	37.98829658923663	40.0	37.0	41.0	33.0	41.0
17	37.95713033996234	40.0	37.0	41.0	33.0	41.0
18	37.77188739189395	39.0	37.0	41.0	33.0	41.0
19	37.548337892871906	39.0	37.0	41.0	33.0	41.0
20	37.428366685447536	39.0	35.0	41.0	33.0	41.0
21	37.41310490652053	39.0	35.0	41.0	33.0	41.0
22	37.534226531587215	39.0	35.0	41.0	33.0	41.0
23	37.540490630630245	39.0	35.0	41.0	33.0	41.0
24	37.534269377681895	39.0	35.0	41.0	33.0	41.0
25	37.26457891929295	39.0	35.0	41.0	33.0	41.0
26	37.33607833979952	39.0	35.0	41.0	33.0	41.0
27	37.34128199799909	39.0	35.0	41.0	33.0	41.0
28	37.20607900391399	39.0	35.0	41.0	33.0	41.0
29	37.13309925083603	39.0	35.0	41.0	33.0	41.0
30	36.92109463202703	39.0	35.0	41.0	32.0	41.0
31	36.79566268983498	39.0	35.0	41.0	31.0	41.0
32	36.56656676385589	39.0	35.0	41.0	31.0	41.0
33	36.24885226023861	39.0	35.0	41.0	30.0	41.0
34	35.97057330216994	39.0	35.0	41.0	28.0	41.0
35	35.79334900072195	39.0	35.0	41.0	27.0	41.0
36	35.62049928554137	39.0	35.0	41.0	25.0	41.0
37	35.498784242063294	39.0	35.0	41.0	25.0	41.0
38	35.3787616193253	39.0	35.0	41.0	24.0	41.0
39	35.3127036528438	39.0	35.0	41.0	24.0	41.0
40	35.21239237596591	39.0	35.0	41.0	23.0	41.0
41	35.0560148418872	38.0	35.0	41.0	23.0	41.0
42	35.04414004674509	38.0	35.0	41.0	23.0	41.0
43	35.01985059566783	38.0	35.0	41.0	23.0	41.0
44	34.88075503388055	38.0	34.0	40.0	23.0	41.0
45	34.9087249644913	38.0	34.0	40.0	23.0	41.0
46	34.83043443797706	38.0	34.0	40.0	23.0	41.0
47	34.74196582167027	38.0	34.0	40.0	23.0	41.0
48	34.66170651710523	38.0	34.0	40.0	23.0	41.0
49	34.601719842240676	38.0	34.0	40.0	22.0	41.0
50	34.45517120228284	38.0	34.0	40.0	22.0	41.0
51	33.310752013230875	36.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	11.0
11	3.0
12	14.0
13	11.0
14	18.0
15	16.0
16	41.0
17	86.0
18	178.0
19	357.0
20	653.0
21	1101.0
22	1531.0
23	2274.0
24	3355.0
25	5683.0
26	8491.0
27	9609.0
28	9438.0
29	8998.0
30	9304.0
31	10846.0
32	13507.0
33	18398.0
34	29487.0
35	32781.0
36	36753.0
37	50944.0
38	90241.0
39	122621.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.34666775210107	19.072082127394292	22.435714790686117	18.145535329818525
2	37.077510727590955	25.89746501080793	22.319387643614753	14.705636617986361
3	27.972287145957363	25.185791378080367	31.879208289862397	14.96271318609987
4	25.212141726311998	22.376587180020007	35.53826477601133	16.87300631765666
5	25.30211852515173	26.682833926394693	30.256626684119308	17.75842086433427
6	23.350907373170205	33.03369631116548	30.257269375539593	13.358126940124727
7	72.00607557622641	5.113038709304243	18.603345851533998	4.277539862935343
8	73.62780026007579	6.470188758470137	15.450087513148395	4.451923468305672
9	67.88878010741516	6.552881721213316	17.03410763367446	8.524230537697065
10	36.54557646206943	26.657768961003626	23.339553158078523	13.457101418848424
11	26.421686979286058	23.222797550060307	32.20590976183998	18.149605708813656
12	23.9477534721404	20.195506730050322	36.115401671426156	19.741338126383127
13	23.04777125326969	21.27994138654247	38.086322026963046	17.58596533322479
14	19.00331414542393	26.254801440485704	35.73128643256989	19.01059798152048
15	17.906668351946394	24.887796789542126	39.13905057338786	18.06648428512362
16	21.594217491061233	24.35307752786603	34.213035067386194	19.839669913686542
17	21.38105817000045	24.745761985659414	33.48936452814667	20.38381531619347
18	21.70497464582347	24.35607675449402	34.05171952089497	19.88722907878754
19	21.27501408565363	26.8846390323638	31.850072945476203	19.990273936506373
20	21.85493597722302	26.38998086921872	33.47801031305499	18.27707284050327
21	21.923918189666807	26.412903529875507	34.24838309550181	17.414795184955878
22	20.694449502663957	22.60559955611446	35.35338387744303	21.346567063778554
23	19.992202010767222	25.49471172076343	34.880577222587604	19.63250904588174
24	21.267087558136794	24.918860208189177	33.44823227724851	20.365819956425522
25	21.595074412954947	26.967974686527256	31.338276344456894	20.098674556060903
26	19.758262333783932	24.796963068808687	32.56624541814575	22.878529179261633
27	20.13380835370308	24.75925850548537	33.13459886415003	21.972334276661517
28	19.180054286001965	25.3111162050357	34.07100026350348	21.43782924545885
29	21.836512156508213	24.90664907120378	32.06387495795727	21.192963814330735
30	21.108557007800133	25.55255394858897	32.84003196318663	20.498857080424262
31	22.377872562860578	26.081488987482516	30.406159554572	21.13447889508491
32	22.475775889217136	29.21332427852532	29.359215230929735	18.951684601327802
33	22.01839382844852	27.466703228667466	29.25702729510462	21.257875647779397
34	23.178023381113867	26.928770509889947	30.202426374342046	19.690779734654136
35	21.48645956292699	28.770509889949807	28.77715103462607	20.965879512497136
36	24.040301036661262	28.88276665802604	28.267068277394188	18.809864027918515
37	21.856864051483868	29.17969009419714	28.66168080944842	20.301765044870574
38	21.125481215200935	30.308684689162295	27.915301840025535	20.650532255611232
39	23.056768933153666	27.183490542795752	28.792789859186307	20.966950664864275
40	22.246977743596116	26.96261892469156	30.630458860251036	20.159944471461287
41	22.18442244535516	26.618564784366317	29.956489790846792	21.240522979431734
42	21.280369847489325	27.43713942333441	30.4376514341659	20.84483929501036
43	22.13172174889189	25.64960035305182	29.76561043902251	22.453067459033775
44	21.664699316819018	25.61789424298449	29.88622219556243	22.831184244634063
45	20.92003419118356	25.26055781330671	29.307157225886755	24.512250769622977
46	23.005353619530965	26.433255424851165	28.76901027663581	21.792380678982063
47	20.35146651470585	25.71965371786275	32.202696304738566	21.726183462692834
48	20.36367765169124	26.51916184469576	30.555692425024688	22.561468078588305
49	20.628252286374725	24.939426333638252	32.56538849625204	21.86693288373498
50	20.573623515650606	24.591730275264737	32.70206753829905	22.132578670785605
51	20.111742614940002	24.71169934038437	30.784490570645712	24.39206747402991
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	778.0
1	809.0
2	840.0
3	7115.0
4	13390.0
5	8729.0
6	4068.0
7	3951.0
8	3834.0
9	3841.0
10	3848.0
11	3721.0
12	3594.0
13	3453.0
14	3312.0
15	3177.0
16	3042.0
17	2927.5
18	2813.0
19	2589.5
20	2366.0
21	2405.5
22	2445.0
23	2538.5
24	2632.0
25	3059.0
26	3872.5
27	4259.0
28	5045.0
29	5831.0
30	6698.0
31	7565.0
32	8594.0
33	9623.0
34	11195.0
35	12767.0
36	13718.0
37	14669.0
38	16068.0
39	17467.0
40	18698.5
41	19930.0
42	21583.0
43	23236.0
44	25572.5
45	27909.0
46	38869.5
47	49830.0
48	46228.0
49	42626.0
50	40776.5
51	38927.0
52	33918.0
53	28909.0
54	26621.5
55	24334.0
56	22581.5
57	20829.0
58	19425.5
59	18022.0
60	16490.5
61	14959.0
62	13084.0
63	11209.0
64	9869.5
65	8530.0
66	7287.5
67	6045.0
68	4832.5
69	3620.0
70	2942.0
71	2264.0
72	1829.0
73	1394.0
74	1082.0
75	589.0
76	408.0
77	331.0
78	254.0
79	164.5
80	75.0
81	55.0
82	35.0
83	30.5
84	26.0
85	18.0
86	10.0
87	5.5
88	1.0
89	2.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	466787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.74940805770661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8856185747662	28.268877732326224
2	8.011338014023808	6.0484653555920325
3	3.2677113362258132	3.7006250593794587
4	2.047027657448831	3.090963293857887
5	1.5248781874675397	2.8781624468504092
6	1.306174656658852	2.9584392065309895
7	1.04010437882321	2.7484327233163373
8	0.9229371428170565	2.7872264652651926
9	0.8418610534637108	2.860178079158314
>10	6.0778478315242985	35.462163565669044
>50	0.043222050956778994	1.1099220205084377
>100	0.023317159068788635	1.9203583120789094
>500	0.00398097837759806	1.023618606147819
>1k	0.002843555983998614	2.2531202331210904
>5k	0.0011374223935994457	2.8894469001978806
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8312	1.7806836951328981	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	5147	1.1026442467335207	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	3168	0.6786821398196607	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG	3093	0.6626148543125665	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	1901	0.407252129986482	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	1230	0.26350348231634557	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	1103	0.2362962121909993	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	952	0.2039474107033829	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	723	0.1548886322883885	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	688	0.14739056571841117	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	670	0.14353441719670856	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	640	0.13710750299387087	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC	576	0.12339675269448379	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	519	0.11118561570909215	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	486	0.10411601008597068	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGT	483	0.10347331866568692	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	469	0.100474092037696	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1423047342792323E-4	0.0	0.0	0.11075715476223631	0.0
2	2.1423047342792323E-4	0.0	0.0	0.5827068877239512	0.0
3	2.1423047342792323E-4	0.0	0.0	0.9556821419619655	0.0
4	2.1423047342792323E-4	0.0	0.0	1.777684468504907	0.0
5	2.1423047342792323E-4	0.0	0.0	3.652843802419519	0.0
6	2.1423047342792323E-4	0.0	0.0	4.879527493267807	0.0
7	2.1423047342792323E-4	0.0	0.0	5.731522086090658	0.0
8	2.1423047342792323E-4	0.0	0.0	6.766041042274099	0.0
9	2.1423047342792323E-4	0.0	0.0	7.280836869921399	0.0
10	2.1423047342792323E-4	0.0	0.0	8.658124583589517	0.0
11	2.1423047342792323E-4	0.0	0.0	9.45527617521482	0.0
12	2.1423047342792323E-4	0.0	0.0	10.771722434429408	0.0
13	2.1423047342792323E-4	0.0	0.0	11.164192661749363	0.0
14	2.1423047342792323E-4	0.0	0.0	11.403273870094926	0.0
15	2.1423047342792323E-4	0.0	0.0	11.683273098865222	0.0
16	2.1423047342792323E-4	0.0	0.0	12.024756473509331	0.0
17	2.1423047342792323E-4	0.0	0.0	12.403730181003327	0.0
18	4.2846094685584647E-4	0.0	0.0	12.849543796206834	0.0
19	4.2846094685584647E-4	0.0	0.0	13.251011703410763	0.0
20	4.2846094685584647E-4	0.0	0.0	13.560360507040684	0.0
21	4.2846094685584647E-4	0.0	0.0	13.89605965890224	0.0
22	4.2846094685584647E-4	0.0	0.0	14.2870302729082	0.0
23	4.2846094685584647E-4	0.0	0.0	14.66471859756163	0.0
24	4.2846094685584647E-4	0.0	0.0	14.959928189945307	0.0
25	4.2846094685584647E-4	0.0	0.0	15.254280860435273	0.0
26	4.2846094685584647E-4	0.0	0.0	15.578625797205149	0.0
27	4.2846094685584647E-4	0.0	0.0	15.91903801948212	0.0
28	4.2846094685584647E-4	0.0	0.0	16.212319537604944	0.0
29	4.2846094685584647E-4	0.0	0.0	16.52059718886773	0.0
30	4.2846094685584647E-4	0.0	0.0	16.920993943704516	0.0
31	4.2846094685584647E-4	0.0	0.0	17.250266181363234	0.0
32	4.2846094685584647E-4	0.0	0.0	17.57675342286739	0.0
33	4.2846094685584647E-4	0.0	0.0	17.92016487177235	0.0
34	4.2846094685584647E-4	0.0	0.0	18.287141672754384	0.0
35	4.2846094685584647E-4	0.0	0.0	18.67532729060578	0.0
36	6.426914202837697E-4	0.0	0.0	19.00524221968478	0.0
37	6.426914202837697E-4	0.0	0.0	19.327659082193804	0.0
38	6.426914202837697E-4	0.0	0.0	19.72312853614175	0.0
39	6.426914202837697E-4	0.0	0.0	20.258062028291278	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	25	3.887233E-5	45.0	21
TACGGGT	25	3.887233E-5	45.0	4
TAGCCGT	25	3.887233E-5	45.0	44
GGACGTA	25	3.887233E-5	45.0	8
TAGTGCG	35	1.2098462E-7	45.0	1
AGTACGG	50	2.1827873E-11	45.0	2
CGGTCTA	65	0.0	44.999996	31
CGTTTTT	4330	0.0	44.012703	1
CGACGGT	75	0.0	42.000004	28
TCACGAC	70	0.0	41.785713	25
ACGGTCT	70	0.0	41.785713	30
CACGGGC	40	3.4523327E-7	39.375	4
GACGGTC	80	0.0	39.375	29
GACGGGT	40	3.4523327E-7	39.375	4
TGTAGCG	40	3.4523327E-7	39.375	1
CACGACG	75	0.0	39.000004	26
CGGGCTT	35	6.240318E-6	38.571426	6
TTGCGCG	35	6.240318E-6	38.571426	14
GCCTAAC	35	6.240318E-6	38.571426	10
TTTACGG	65	9.094947E-12	38.07692	2
>>END_MODULE