SRR2935432.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935432.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481021
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	14875	3.0923805821367467	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	14434	3.000700593113398	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	13278	2.760378445015914	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3871	0.8047465703160568	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3617	0.7519422228967134	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	3415	0.7099482143191254	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	2884	0.5995580234542774	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	2503	0.5203515023252624	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	2177	0.4525789934327192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2130	0.44280811024882494	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	2101	0.4367792674332306	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	1278	0.26568486614929493	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1153	0.23969847470276767	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	942	0.1958334459410296	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	939	0.19520977254631294	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATG	864	0.17961793767839657	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	704	0.14635535662684165	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACAGATCGT	571	0.11870583612773662	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	569	0.11829005386459218	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTAT	544	0.11309277557528673	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	538	0.11184542878585342	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	530	0.11018229973327569	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	513	0.10664815049654795	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	497	0.10332189239139247	Illumina Single End Adapter 2 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTTCG	35	1.2099554E-7	45.000004	11
CGGTCTA	95	0.0	45.000004	31
CACAACG	60	0.0	45.000004	12
TCACGAC	100	0.0	45.0	25
CATGCGG	40	6.8012014E-9	45.0	2
CGACGCC	50	2.1827873E-11	45.0	45
CGCATCG	40	6.8012014E-9	45.0	21
AAACGCC	25	3.8874117E-5	45.0	23
ACTACGC	25	3.8874117E-5	45.0	23
TCCGTGC	20	7.0291926E-4	45.0	45
TCTCCGT	20	7.0291926E-4	45.0	43
CCGACTC	25	3.8874117E-5	45.0	27
ATAACGC	45	3.8380676E-10	45.0	11
TAGGCGT	25	3.8874117E-5	45.0	1
CCGAACC	25	3.8874117E-5	45.0	18
ATACGCA	20	7.0291926E-4	45.0	38
CGGTAAT	20	7.0291926E-4	45.0	6
CGCCCTA	25	3.8874117E-5	45.0	26
TTTTACG	20	7.0291926E-4	45.0	1
TGCGTAG	20	7.0291926E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content