##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935432.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481021
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.535180376740307	31.0	31.0	34.0	30.0	34.0
2	31.598786747356144	31.0	31.0	34.0	30.0	34.0
3	31.720292877026157	31.0	31.0	34.0	30.0	34.0
4	35.50478877221577	37.0	35.0	37.0	33.0	37.0
5	35.49398674901927	37.0	35.0	37.0	33.0	37.0
6	35.39696395791452	37.0	35.0	37.0	33.0	37.0
7	35.68031541242482	37.0	35.0	37.0	35.0	37.0
8	35.35900511620075	37.0	35.0	37.0	33.0	37.0
9	37.200797886162974	39.0	37.0	39.0	34.0	39.0
10	36.59214878352504	39.0	35.0	39.0	32.0	39.0
11	36.63166888763692	39.0	35.0	39.0	32.0	39.0
12	36.64965354942923	39.0	35.0	39.0	32.0	39.0
13	36.520975175719975	39.0	35.0	39.0	32.0	39.0
14	37.488465160564715	39.0	36.0	41.0	32.0	41.0
15	37.45324424505375	39.0	36.0	41.0	32.0	41.0
16	37.612416089942016	39.0	36.0	41.0	33.0	41.0
17	37.39537982749194	39.0	36.0	41.0	32.0	41.0
18	37.40182029474805	39.0	36.0	41.0	32.0	41.0
19	37.267790803312124	39.0	36.0	40.0	32.0	41.0
20	37.16291596416789	39.0	35.0	41.0	32.0	41.0
21	37.23919953598699	39.0	35.0	40.0	32.0	41.0
22	37.38918259285977	39.0	35.0	41.0	32.0	41.0
23	37.357728664652896	39.0	35.0	41.0	32.0	41.0
24	37.31065379681968	39.0	35.0	41.0	32.0	41.0
25	37.174298003621466	39.0	35.0	41.0	32.0	41.0
26	37.19353001220321	39.0	35.0	41.0	32.0	41.0
27	37.187783901326554	39.0	35.0	41.0	32.0	41.0
28	37.02941659511747	39.0	35.0	41.0	31.0	41.0
29	37.04576931152694	39.0	35.0	41.0	31.0	41.0
30	36.94192353348399	39.0	35.0	41.0	31.0	41.0
31	37.04039948359843	39.0	35.0	41.0	31.0	41.0
32	36.87790969625027	39.0	35.0	41.0	31.0	41.0
33	36.752877732988786	39.0	35.0	41.0	31.0	41.0
34	36.687710099974844	39.0	35.0	41.0	31.0	41.0
35	36.64410285621626	39.0	35.0	41.0	31.0	41.0
36	36.638496032397754	39.0	35.0	41.0	31.0	41.0
37	36.543113502321106	39.0	35.0	41.0	30.0	41.0
38	36.560547252614754	39.0	35.0	41.0	30.0	41.0
39	36.38099999792109	39.0	35.0	41.0	30.0	41.0
40	36.299824747776086	39.0	35.0	41.0	30.0	41.0
41	36.049320092054195	39.0	35.0	40.0	29.0	41.0
42	36.195644265011296	39.0	35.0	41.0	30.0	41.0
43	36.21673066248667	39.0	35.0	40.0	30.0	41.0
44	36.17671161965902	39.0	35.0	40.0	30.0	41.0
45	36.14339082909062	39.0	35.0	40.0	30.0	41.0
46	36.044634225948556	39.0	35.0	40.0	30.0	41.0
47	35.866309371108535	38.0	35.0	40.0	29.0	41.0
48	35.813577785585245	38.0	35.0	40.0	29.0	41.0
49	35.821043156120005	38.0	35.0	40.0	29.0	41.0
50	35.705033667968756	38.0	35.0	40.0	28.0	41.0
51	34.460185729936946	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	8.0
11	9.0
12	8.0
13	7.0
14	11.0
15	17.0
16	38.0
17	70.0
18	154.0
19	378.0
20	678.0
21	1161.0
22	1720.0
23	2386.0
24	3108.0
25	3964.0
26	4899.0
27	5804.0
28	6154.0
29	6960.0
30	8629.0
31	11190.0
32	14976.0
33	21204.0
34	32780.0
35	39545.0
36	40652.0
37	56339.0
38	97323.0
39	120795.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.840487213655955	17.549337762800377	22.450786971878568	11.159388051665104
2	27.74348729057567	36.255173890537	21.806532355136262	14.194806463751064
3	25.47456348059648	35.90716413628511	25.154203246843693	13.464069136274714
4	23.670484240812772	21.0564611524237	39.18623095457371	16.08682365218982
5	31.61462805158195	24.872094981300194	24.65609609559666	18.8571808715212
6	20.649826099068438	40.6125720082907	25.90760070766141	12.83000118497945
7	67.76793528764856	5.198317744963318	22.229590807885728	4.804156159502392
8	67.25049426116531	14.875857810781651	11.9406429241135	5.933005003939537
9	63.819874807960566	5.283137326644783	12.156641809817035	18.740346055577614
10	38.76878556237669	24.199775061795638	20.547543662334906	16.483895713492757
11	28.830757908698374	23.80249510936113	26.99923703954713	20.367509942393365
12	22.732063672895777	19.80911436299039	33.10479168269161	24.354030281422226
13	23.449495967951503	21.878878468923393	38.34302452491679	16.328601038208312
14	19.30497836892776	31.379087399510624	28.722030846886103	20.593903384675514
15	15.055683639591619	24.305591647765898	39.62508913332266	21.01363557931982
16	17.85348248829053	25.84460969479503	28.756540774727092	27.545367042187348
17	17.541853682063778	26.39094758856682	32.00920541930602	24.057993310063384
18	17.963249005760662	25.771224125350034	31.198014223911226	25.06751264497808
19	17.623347005640085	28.342629531766804	28.57941753062756	25.454605931965546
20	20.314913486105596	27.234361909355307	33.506437348889136	18.94428725564996
21	19.51952201671029	32.7239351296513	30.11074360578852	17.645799247849887
22	17.289265957203533	22.92436296960008	32.795657570043716	26.99071350315267
23	17.65952006253365	30.40511744809478	31.502366840532947	20.432995648838617
24	21.19720344849809	26.142725577469587	27.607110708264297	25.05296026576802
25	17.862213915816564	34.66231204043066	25.89221676392507	21.5832572798277
26	17.411298051436425	24.45506537136632	30.309903309834706	27.82373326736255
27	20.949605110795577	27.216691163171667	27.73018225815505	24.1035214678777
28	15.067117651828092	25.44712185122895	33.274638737186116	26.21112175975685
29	18.98170765933296	24.04073834614289	28.97586591853578	28.00168807598837
30	17.40630866427869	27.094451177807205	33.31871165707942	22.180528500834683
31	21.33794574457248	24.997869115901388	24.185638464848726	29.478546674677403
32	22.76303945150004	31.06142975046827	24.205180231216517	21.97035056681517
33	18.167813879227726	25.197444602210716	24.278981582924654	32.35575993563691
34	19.93010700156542	27.223551570513553	25.650023595643432	27.196317832277593
35	20.63319480854266	25.645657881880418	27.253487893459955	26.467659416116966
36	18.597316957055927	28.776082541094876	24.62075460322938	28.005845898619814
37	17.10050080973596	28.69916282241316	32.51500454242123	21.68533182542966
38	18.600643215161085	26.314443652148245	23.662376486681456	31.42253664600922
39	19.52534296839431	31.52398751821646	25.90198764710896	23.048681866280265
40	21.229426573891786	23.346589857823254	29.50349361046607	25.920489957818887
41	17.499443891223045	32.25576430135067	25.517596944831933	24.727194862594356
42	21.84935792824014	24.89142885653641	31.188867014122046	22.070346201101408
43	21.09221842705412	26.625448784980282	25.746692971824515	26.53563981614108
44	20.1165853465857	23.534731331896115	30.036942254080383	26.311741067437804
45	19.244482049640244	21.87181017044994	27.95907039401606	30.924637385893757
46	25.914669006134865	26.07495306857705	25.671020599932227	22.339357325355856
47	16.597196380199616	23.78378490751963	36.07493227946389	23.54408643281686
48	19.74653913238715	27.010255269520457	26.627943478559146	26.615262119533245
49	18.820799923496065	21.86453398084491	34.74900264229628	24.565663453362742
50	20.098706709270488	21.24938412252272	31.259342107725026	27.392567060481767
51	18.636400489791505	21.72462324929681	27.906473937728286	31.732502323183397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2482.0
1	1944.5
2	1407.0
3	3403.0
4	5399.0
5	3370.5
6	1342.0
7	1348.0
8	1354.0
9	1342.0
10	1330.0
11	1345.5
12	1361.0
13	1370.0
14	1379.0
15	1383.5
16	1388.0
17	1376.5
18	1365.0
19	1309.0
20	1253.0
21	1313.0
22	1373.0
23	1708.5
24	2044.0
25	2427.5
26	3192.5
27	3574.0
28	4250.0
29	4926.0
30	5515.0
31	6104.0
32	7042.5
33	7981.0
34	9245.5
35	10510.0
36	12047.5
37	13585.0
38	14574.0
39	15563.0
40	16843.0
41	18123.0
42	20772.0
43	23421.0
44	27250.0
45	31079.0
46	63694.5
47	96310.0
48	74965.0
49	53620.0
50	50705.0
51	47790.0
52	39240.5
53	30691.0
54	26784.0
55	22877.0
56	20942.5
57	19008.0
58	16498.5
59	13989.0
60	12606.0
61	11223.0
62	9707.5
63	8192.0
64	6944.5
65	5697.0
66	4755.0
67	3813.0
68	3089.5
69	2366.0
70	2061.5
71	1757.0
72	1526.5
73	1296.0
74	980.5
75	458.0
76	251.0
77	207.0
78	163.0
79	115.0
80	67.0
81	54.5
82	42.0
83	35.0
84	28.0
85	17.5
86	7.0
87	7.5
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	481021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.185530248531904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56083483100441	23.997800048090838
2	10.391720486292995	6.689260680917437
3	3.820286684959706	3.6887385797050287
4	1.869045868582155	2.4062492935657813
5	1.1499532725112986	1.850592791840533
6	0.8455220265849241	1.6328144857469447
7	0.6580780770438982	1.4826414298214468
8	0.5777192740155136	1.4875360935188966
9	0.47868012659116427	1.3865916324393899
>10	5.482214199499587	34.86090705446318
>50	0.10241927406432687	2.2102695638289984
>100	0.048616744017876676	2.777962926409582
>500	0.006482232535716889	1.400769289366424
>1k	0.006482232535716889	5.242766081693308
>5k	0.0	0.0
>10k+	0.001944669760715067	8.88510004859218
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	14875	3.0923805821367467	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	14434	3.000700593113398	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	13278	2.760378445015914	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3871	0.8047465703160568	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3617	0.7519422228967134	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	3415	0.7099482143191254	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	2884	0.5995580234542774	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	2503	0.5203515023252624	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	2177	0.4525789934327192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2130	0.44280811024882494	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	2101	0.4367792674332306	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	1278	0.26568486614929493	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1153	0.23969847470276767	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	942	0.1958334459410296	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	939	0.19520977254631294	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATG	864	0.17961793767839657	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	704	0.14635535662684165	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACAGATCGT	571	0.11870583612773662	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	569	0.11829005386459218	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTAT	544	0.11309277557528673	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	538	0.11184542878585342	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	530	0.11018229973327569	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	513	0.10664815049654795	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	497	0.10332189239139247	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0789113157221827E-4	0.0	0.0	0.2557060918338285	0.0
2	2.0789113157221827E-4	0.0	0.0	1.5138632201088933	0.0
3	2.0789113157221827E-4	0.0	0.0	2.0294332264079946	0.0
4	2.0789113157221827E-4	0.0	0.0	3.3418499400234087	0.0
5	2.0789113157221827E-4	0.0	0.0	7.689892956856354	0.0
6	2.0789113157221827E-4	0.0	0.0	8.960939335288895	0.0
7	2.0789113157221827E-4	0.0	0.0	10.394140796347768	0.0
8	2.0789113157221827E-4	0.0	0.0	12.080137873398458	0.0
9	2.0789113157221827E-4	0.0	0.0	12.493009660700885	0.0
10	2.0789113157221827E-4	0.0	0.0	16.28182553360456	0.0
11	2.0789113157221827E-4	0.0	0.0	17.60214211021972	0.0
12	2.0789113157221827E-4	0.0	0.0	21.95600607873669	0.0
13	4.1578226314443654E-4	0.0	0.0	22.64765987347746	0.0
14	4.1578226314443654E-4	0.0	0.0	22.972593712124834	0.0
15	4.1578226314443654E-4	0.0	0.0	23.745740830441914	0.0
16	6.236733947166548E-4	0.0	0.0	24.485209585444295	0.0
17	6.236733947166548E-4	0.0	0.0	25.292450849339218	0.0
18	6.236733947166548E-4	0.0	0.0	26.189916864336485	0.0
19	6.236733947166548E-4	0.0	0.0	27.230204086723866	0.0
20	6.236733947166548E-4	0.0	0.0	27.832048912625435	0.0
21	6.236733947166548E-4	0.0	0.0	28.486282303683208	0.0
22	6.236733947166548E-4	0.0	0.0	29.28770261589411	0.0
23	6.236733947166548E-4	0.0	0.0	29.981019539687455	0.0
24	6.236733947166548E-4	0.0	0.0	30.599495656114804	0.0
25	6.236733947166548E-4	0.0	0.0	31.099681718677562	0.0
26	6.236733947166548E-4	0.0	0.0	31.642277572081053	0.0
27	6.236733947166548E-4	0.0	0.0	32.27717708790261	0.0
28	6.236733947166548E-4	0.0	0.0	32.77008696086034	0.0
29	6.236733947166548E-4	0.0	0.0	33.322661588579294	0.0
30	6.236733947166548E-4	0.0	0.0	33.88417553495585	0.0
31	6.236733947166548E-4	0.0	0.0	34.452341997542725	0.0
32	6.236733947166548E-4	0.0	0.0	34.903673644186014	0.0
33	6.236733947166548E-4	0.0	0.0	35.402196577696195	0.0
34	6.236733947166548E-4	0.0	0.0	35.907372027416685	0.0
35	6.236733947166548E-4	0.0	0.0	36.4460179493203	0.0
36	6.236733947166548E-4	0.0	0.0	36.92167285835754	0.0
37	6.236733947166548E-4	0.0	0.0	37.38776477534245	0.0
38	6.236733947166548E-4	0.0	0.0	37.85988553514296	0.0
39	6.236733947166548E-4	0.0	0.0	38.455701518228935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTTCG	35	1.2099554E-7	45.000004	11
CGGTCTA	95	0.0	45.000004	31
CACAACG	60	0.0	45.000004	12
TCACGAC	100	0.0	45.0	25
CATGCGG	40	6.8012014E-9	45.0	2
CGACGCC	50	2.1827873E-11	45.0	45
CGCATCG	40	6.8012014E-9	45.0	21
AAACGCC	25	3.8874117E-5	45.0	23
ACTACGC	25	3.8874117E-5	45.0	23
TCCGTGC	20	7.0291926E-4	45.0	45
TCTCCGT	20	7.0291926E-4	45.0	43
CCGACTC	25	3.8874117E-5	45.0	27
ATAACGC	45	3.8380676E-10	45.0	11
TAGGCGT	25	3.8874117E-5	45.0	1
CCGAACC	25	3.8874117E-5	45.0	18
ATACGCA	20	7.0291926E-4	45.0	38
CGGTAAT	20	7.0291926E-4	45.0	6
CGCCCTA	25	3.8874117E-5	45.0	26
TTTTACG	20	7.0291926E-4	45.0	1
TGCGTAG	20	7.0291926E-4	45.0	1
>>END_MODULE