SRR2935429.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935429.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302850
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	24701	8.156182928842663	RNA PCR Primer, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	23056	7.613009740795773	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	15218	5.024929833250784	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8418	2.7795938583457156	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5857	1.9339607066204392	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	4653	1.536404160475483	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	3722	1.2289912497936273	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	3663	1.2095096582466567	No Hit
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	3204	1.0579494799405647	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTC	2933	0.9684662374112597	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCAAAGCT	2790	0.9212481426448738	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	2510	0.8287931319134886	RNA PCR Primer, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTA	2253	0.7439326399207529	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	1780	0.5877497110780915	RNA PCR Primer, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	1112	0.36717847119035824	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	847	0.27967640746244016	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGT	800	0.26415717351824336	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	780	0.25755324418028724	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	757	0.24995872544163777	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATG	735	0.24269440316988608	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTAT	574	0.1895327719993396	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTT	554	0.18292884266138354	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCG	550	0.1816080567937923	No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	522	0.1723625557206538	Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCCAAAGC	381	0.1258048538880634	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTC	372	0.12283308568598317	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAAAGCTCGAATGC	340	0.11226679874525342	No Hit
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	339	0.11193660227835561	RNA PCR Primer, Index 13 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	336	0.1109460128776622	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	155	0.0	45.000004	34
CTATCCC	45	3.8198777E-10	45.000004	34
GCGAAAA	60	0.0	45.000004	33
CGGCCAA	30	2.1601882E-6	45.000004	31
CATTGGG	20	7.0250494E-4	45.000004	3
CGCGCCT	20	7.0250494E-4	45.000004	17
TTACTCC	20	7.0250494E-4	45.000004	28
TACGGTT	40	6.7866495E-9	45.000004	29
AGGAACC	20	7.0250494E-4	45.000004	24
CGACACC	20	7.0250494E-4	45.000004	34
TAGCCGT	20	7.0250494E-4	45.000004	44
CTGCGAT	30	2.1601882E-6	45.000004	31
GTAGCCG	20	7.0250494E-4	45.000004	43
CCCGTAA	30	2.1601882E-6	45.000004	41
GCCGACC	20	7.0250494E-4	45.000004	35
AGCGCCC	20	7.0250494E-4	45.000004	38
CTGTTAT	20	7.0250494E-4	45.000004	31
TACTCCT	45	3.8198777E-10	45.000004	29
CGGTAAA	20	7.0250494E-4	45.000004	31
GGCGAAA	60	0.0	45.000004	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content