FastQCFastQC Report
Sat 14 Jan 2017
SRR2935428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312734
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC40331.28959435174941No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG39731.2704087179519974No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC37701.2054973236040851No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31311.001170323661642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14670.4690887463467356No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC11090.354614464688841TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG9470.30281325343582727TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT8960.2865054647080266No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT7670.24525635204358975No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT6620.21168149289811788No Hit
CGTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT5800.18546112670832082No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA5760.18418208445516No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC5280.16883357741722999TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTC5260.16819405629064954No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT4300.13749704221478956TruSeq Adapter, Index 14 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT4270.13653776052491895No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC3150.10072457743641561No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG253.8842725E-545.02
TAACGCG207.025402E-445.01
TATTGCG207.025402E-445.01
CGGTCTA253.8842725E-545.031
ACGTAGG453.8198777E-1045.02
GCTACGA351.2081364E-745.010
ATACGAG253.8842725E-545.01
TATCGCG207.025402E-445.01
TAATGCG207.025402E-445.01
GTTATCG207.025402E-445.045
CTAGCGG351.2081364E-745.02
CGGGTTG302.1604155E-644.9999966
CGGCGAA302.1604155E-644.99999631
TAGATCG302.1604155E-644.9999961
CGTTTTT14800.043.479731
GGCGACT750.042.010
CGACTGG556.002665E-1140.9090922
ACGGCTG5400.040.4166648
TACGGCT5450.040.0458687
CAGTGCG451.920671E-840.01
Produced by FastQC (version 0.11.5)